ES2439167B1 - Compuestos con funcionalidad magnética, implantes o geles derivados de ellos, y el uso de ambos para determinar la actividad enzimática de una enzima - Google Patents

Compuestos con funcionalidad magnética, implantes o geles derivados de ellos, y el uso de ambos para determinar la actividad enzimática de una enzima Download PDF

Info

Publication number
ES2439167B1
ES2439167B1 ES201230972A ES201230972A ES2439167B1 ES 2439167 B1 ES2439167 B1 ES 2439167B1 ES 201230972 A ES201230972 A ES 201230972A ES 201230972 A ES201230972 A ES 201230972A ES 2439167 B1 ES2439167 B1 ES 2439167B1
Authority
ES
Spain
Prior art keywords
enzyme
enzymatic activity
magnetic
implants
compounds
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES201230972A
Other languages
English (en)
Other versions
ES2439167A1 (es
Inventor
Marisela VÉLEZ TIRADO
Julian Daich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to ES201230972A priority Critical patent/ES2439167B1/es
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to EP13806520.6A priority patent/EP2865394A4/en
Priority to AU2013279221A priority patent/AU2013279221A1/en
Priority to CA 2877058 priority patent/CA2877058A1/en
Priority to JP2015517814A priority patent/JP2015522568A/ja
Priority to CN201380042510.9A priority patent/CN104540529A/zh
Priority to US14/409,584 priority patent/US20150174272A1/en
Priority to PCT/ES2013/070407 priority patent/WO2013190165A1/es
Publication of ES2439167A1 publication Critical patent/ES2439167A1/es
Application granted granted Critical
Publication of ES2439167B1 publication Critical patent/ES2439167B1/es
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/06—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
    • A61K49/18—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes
    • A61K49/1818—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles
    • A61K49/1821—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles coated or functionalised microparticles or nanoparticles
    • A61K49/1824—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles coated or functionalised microparticles or nanoparticles coated or functionalised nanoparticles
    • A61K49/1827—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles coated or functionalised microparticles or nanoparticles coated or functionalised nanoparticles having a (super)(para)magnetic core, being a solid MRI-active material, e.g. magnetite, or composed of a plurality of MRI-active, organic agents, e.g. Gd-chelates, or nuclei, e.g. Eu3+, encapsulated or entrapped in the core of the coated or functionalised nanoparticle
    • A61K49/1851—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles coated or functionalised microparticles or nanoparticles coated or functionalised nanoparticles having a (super)(para)magnetic core, being a solid MRI-active material, e.g. magnetite, or composed of a plurality of MRI-active, organic agents, e.g. Gd-chelates, or nuclei, e.g. Eu3+, encapsulated or entrapped in the core of the coated or functionalised nanoparticle having a (super)(para)magnetic core coated or functionalised with an organic macromolecular compound, i.e. oligomeric, polymeric, dendrimeric organic molecule
    • A61K49/1863—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles coated or functionalised microparticles or nanoparticles coated or functionalised nanoparticles having a (super)(para)magnetic core, being a solid MRI-active material, e.g. magnetite, or composed of a plurality of MRI-active, organic agents, e.g. Gd-chelates, or nuclei, e.g. Eu3+, encapsulated or entrapped in the core of the coated or functionalised nanoparticle having a (super)(para)magnetic core coated or functionalised with an organic macromolecular compound, i.e. oligomeric, polymeric, dendrimeric organic molecule the organic macromolecular compound being a polysaccharide or derivative thereof, e.g. chitosan, chitin, cellulose, pectin, starch
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/06—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
    • A61K49/18—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes
    • A61K49/1818—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles
    • A61K49/1821—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles coated or functionalised microparticles or nanoparticles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/06—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
    • A61K49/08—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by the carrier
    • A61K49/10—Organic compounds
    • A61K49/14—Peptides, e.g. proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/06—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
    • A61K49/08—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by the carrier
    • A61K49/085—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by the carrier conjugated systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/06—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
    • A61K49/08—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by the carrier
    • A61K49/10—Organic compounds
    • A61K49/12—Macromolecular compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/06—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
    • A61K49/18—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/06—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
    • A61K49/18—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes
    • A61K49/1818—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles
    • A61K49/1821—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles coated or functionalised microparticles or nanoparticles
    • A61K49/1824—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles coated or functionalised microparticles or nanoparticles coated or functionalised nanoparticles
    • A61K49/1827—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles coated or functionalised microparticles or nanoparticles coated or functionalised nanoparticles having a (super)(para)magnetic core, being a solid MRI-active material, e.g. magnetite, or composed of a plurality of MRI-active, organic agents, e.g. Gd-chelates, or nuclei, e.g. Eu3+, encapsulated or entrapped in the core of the coated or functionalised nanoparticle
    • A61K49/1866—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles coated or functionalised microparticles or nanoparticles coated or functionalised nanoparticles having a (super)(para)magnetic core, being a solid MRI-active material, e.g. magnetite, or composed of a plurality of MRI-active, organic agents, e.g. Gd-chelates, or nuclei, e.g. Eu3+, encapsulated or entrapped in the core of the coated or functionalised nanoparticle the nanoparticle having a (super)(para)magnetic core coated or functionalised with a peptide, e.g. protein, polyamino acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/10—Musculoskeletal or connective tissue disorders

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Nanotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Radiology & Medical Imaging (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Materials For Medical Uses (AREA)
  • Magnetic Resonance Imaging Apparatus (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Compuestos con funcionalidad magnética, implantes o geles derivados de ellos, y el uso de ambos para determinar la actividad enzimática de una enzima.#Implante o gel que comprende al menos un compuesto con funcionalidad magnética que comprende a su vez un sustrato de al menos una enzima implicada en la regeneración de la matriz extracelular y una pluralidad de partículas magnéticas. Así como, compuesto con funcionalidad magnética que comprende un sustrato de al menos una enzima implicada en la regeneración de la matriz extracelular y una pluralidad de partículas magnéticas, preferiblemente sin recubrimiento o modificación de superficie o funcionalizadas con grupos carboxílicos, para fabricar un implante o gel para monitorizar una actividad enzimática implicada en la regeneración de la matriz extracelular. Preferiblemente el sustrato se selecciona del grupo que consiste en colágeno, condroitín sulfato y ácido hialurónico y las partículas magnéticas son partículas superparamagnéticas.

Description



imagen1
imagen2
imagen3
imagen4
imagen5
imagen6
imagen7
imagen8
imagen9
imagen10
imagen11
imagen12
imagen13
imagen14
imagen15
imagen16
imagen17
imagen18
imagen19
imagen20
imagen21
imagen22
imagen23
imagen24

Claims (1)



  1. imagen1
    imagen2
    imagen3
ES201230972A 2012-06-21 2012-06-21 Compuestos con funcionalidad magnética, implantes o geles derivados de ellos, y el uso de ambos para determinar la actividad enzimática de una enzima Expired - Fee Related ES2439167B1 (es)

Priority Applications (8)

Application Number Priority Date Filing Date Title
ES201230972A ES2439167B1 (es) 2012-06-21 2012-06-21 Compuestos con funcionalidad magnética, implantes o geles derivados de ellos, y el uso de ambos para determinar la actividad enzimática de una enzima
AU2013279221A AU2013279221A1 (en) 2012-06-21 2013-06-21 Compounds having magnetic functionality, implants or gels derived from same, and use of both in order to determine the enzyme activity of an enzyme
CA 2877058 CA2877058A1 (en) 2012-06-21 2013-06-21 Compounds with magnetic functionality, implants or gels derived thereof, and the use of both to measure the enzyme activity of an enzyme
JP2015517814A JP2015522568A (ja) 2012-06-21 2013-06-21 磁気機能性を有する化合物、それから派生したインプラント又はゲル、及び酵素の酵素活性を測定するための両方の使用
EP13806520.6A EP2865394A4 (en) 2012-06-21 2013-06-21 MAGNETICALLY FUNCTIONALITY COMPOUNDS, IMPLANTS OR GELS DERIVED THEREFROM, AND USE THEREOF FOR DETERMINING THE ENZYMATIC ACTIVITY OF AN ENZYME
CN201380042510.9A CN104540529A (zh) 2012-06-21 2013-06-21 具有磁功能性的化合物、得自其的植入物或凝胶以及二者用于测量酶的酶活性的用途
US14/409,584 US20150174272A1 (en) 2012-06-21 2013-06-21 Compounds having magnetic functionality, implants or gels derived from same, and use of both in order to determine the enzyme activity of an enzyme
PCT/ES2013/070407 WO2013190165A1 (es) 2012-06-21 2013-06-21 Compuestos con funcionalidad magnética, implantes o geles derivados de ellos, y el uso de ambos para determinar la actividad enzimática de una enzima

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201230972A ES2439167B1 (es) 2012-06-21 2012-06-21 Compuestos con funcionalidad magnética, implantes o geles derivados de ellos, y el uso de ambos para determinar la actividad enzimática de una enzima

Publications (2)

Publication Number Publication Date
ES2439167A1 ES2439167A1 (es) 2014-01-21
ES2439167B1 true ES2439167B1 (es) 2014-11-17

Family

ID=49768169

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201230972A Expired - Fee Related ES2439167B1 (es) 2012-06-21 2012-06-21 Compuestos con funcionalidad magnética, implantes o geles derivados de ellos, y el uso de ambos para determinar la actividad enzimática de una enzima

Country Status (8)

Country Link
US (1) US20150174272A1 (es)
EP (1) EP2865394A4 (es)
JP (1) JP2015522568A (es)
CN (1) CN104540529A (es)
AU (1) AU2013279221A1 (es)
CA (1) CA2877058A1 (es)
ES (1) ES2439167B1 (es)
WO (1) WO2013190165A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9835569B2 (en) 2013-09-25 2017-12-05 Toyota Jidosha Kabushiki Kaisha Magnetic measurement system and apparatus utilizing X-ray to measure comparatively thick magnetic materials
JP6411722B2 (ja) * 2013-09-25 2018-10-24 トヨタ自動車株式会社 磁気特性測定方法
CN106456663A (zh) * 2014-06-11 2017-02-22 国际科学私人有限公司 治疗或预防关节退化的装置和方法
ES2574655B1 (es) * 2014-11-21 2017-04-20 Universidad De Granada Elaboración de tejidos artificiales que comprenden partículas magnéticas
KR102573045B1 (ko) * 2017-12-04 2023-09-01 에보니크 오퍼레이션즈 게엠베하 두 가지 재료의 조합을 사용한 esr 신호의 마스킹 및 후속 시각화
CN109517781B (zh) * 2018-11-23 2022-07-08 四川大学 具有可洗脱超顺磁凝胶涂层的细胞-微球及其制备方法与应用
CN110246641B (zh) * 2019-05-05 2021-08-27 桂林理工大学 一种制备良好分散性的降解透明质酸修饰的超顺磁性氧化铁纳米粒子的方法
EP3805402A1 (en) * 2019-10-10 2021-04-14 ETH Zurich Non-invasive method for detection of enzyme activity in vivo, substrates and a device therefore

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ507540A (en) * 1998-04-28 2002-07-26 Jagotec Ag Hyaluronic acid based imaging agents containing gadolinium or iron oxide
JP2002517085A (ja) * 1998-05-26 2002-06-11 バー−イラン ユニバーシティ 磁性金属酸化物ナノ粒子の核生成および成長ならびにその使用
WO2001089584A2 (en) * 2000-05-23 2001-11-29 Amersham Health As Contrast agents
WO2004009082A1 (en) * 2002-07-19 2004-01-29 The General Hospital Corporation Oxime conjugates and methods for their formation and use
GB0510461D0 (en) * 2005-05-23 2005-06-29 Namosphere Ltd Assay particles
EP1969372A4 (en) * 2005-12-23 2009-04-22 Perkinelmer Las Inc METHOD AND COMPOSITIONS FOR DETECTING ENZYMATIC ACTIVITY
WO2010120905A2 (en) * 2009-04-15 2010-10-21 Board Of Trustees Of Michigan State University Novel nano-probes for molecular imaging and targeted therapy of diseases
US9823325B2 (en) * 2009-12-14 2017-11-21 Kevin M. Bennett Methods and compositions relating to reporter gels for use in MRI techniques
US20110182815A1 (en) * 2010-01-22 2011-07-28 Julian Daich Method for the detection of enzymatic activity with magnetically functionalized substrates

Also Published As

Publication number Publication date
US20150174272A1 (en) 2015-06-25
CA2877058A1 (en) 2013-12-27
EP2865394A4 (en) 2016-01-27
ES2439167A1 (es) 2014-01-21
WO2013190165A1 (es) 2013-12-27
AU2013279221A1 (en) 2015-01-22
CN104540529A (zh) 2015-04-22
JP2015522568A (ja) 2015-08-06
EP2865394A1 (en) 2015-04-29

Similar Documents

Publication Publication Date Title
ES2439167B1 (es) Compuestos con funcionalidad magnética, implantes o geles derivados de ellos, y el uso de ambos para determinar la actividad enzimática de una enzima
ES2610199T3 (es) Método para la producción de celulosa microfibrilada deshidratada
BR112015031094A2 (pt) células progenitoras renais
BR112012020958A2 (pt) "meio de cultura para a criação e/ou diferenciação de células da linhagem hemapo
IL242289B (en) Interneuronal cells of the cerebral cortex and other neurons produced by direct differentiation of pluripotent and multipotent cells
BR112014032074A2 (pt) construções de tecido conjuntivo tridimensionais engendradas e métodos de fabricar as mesmas
BRPI0814894A2 (pt) Diferenciação de células-tronco embrionárias de ser humano.
UY33970A (es) Derivados de ácido 3-fenilpropiónico ramificados y su uso
BR112014016647A8 (pt) estruturas fibrosas compreendendo partículas e métodos de produção das mesmas
JP2011004745A5 (es)
CR20120162A (es) Capa superior protectora removible para revestimientos de uñas artificiales y métodos para su aplicación
BR112014028413A2 (pt) usos terapêuticos de proteínas do fator de crescimento de fibroblastos 21 .
CL2013003018A1 (es) Emulsion de cera acuosa que comprende agua, hidrocarburo parafinico y un componente de cera con un componente de cera de olefinas sinteticas del grupo que consiste en una cera de alfa olefina normal, una cera con olefina de 20 o mas carbonos y combinaciones de las mismas; composicion de yeso; metodo de fabricacion; y sustituto de cera de lignito.
JP2011519825A5 (es)
Gonnerman et al. The promotion of HL-1 cardiomyocyte beating using anisotropic collagen-GAG scaffolds
ECSP13013092A (es) Ensayo de lisiloxidasa-homólogo 2 y métodos de uso del mismo
BR112015023641A2 (pt) uso de micropartículas e células endotelial com descelularização de órgãos e tecidos
CA2835730C (en) Molecular markers in prostate cancer
CL2014002975A1 (es) Método in vitro para el diagnostico y vigilancia del cáncer, que comprende determinar la razón entre las células treg y otro grupo de células t, seleccionadas de th17, th1 y/o th2.
BR112014026430A2 (pt) estruturas fibrosas e métodos de fabricação das mesmas
EA201991452A1 (ru) Конформное покрытие биологических поверхностей
BR112012033280A2 (pt) sistema e método comunicação, programa, e, meio de armazenamento da informação
CR20130106A (es) Nuevos moduladores de trpv3
JP2014511827A5 (es)
FR2981072B1 (fr) Compose sensible au ph, utilisation, composition et procede de traitement le mettant en œuvre

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 2439167

Country of ref document: ES

Kind code of ref document: B1

Effective date: 20141117

FD2A Announcement of lapse in spain

Effective date: 20210915