ES2398984T3 - RT-PCR rápida en una etapa - Google Patents

RT-PCR rápida en una etapa Download PDF

Info

Publication number
ES2398984T3
ES2398984T3 ES08006778T ES08006778T ES2398984T3 ES 2398984 T3 ES2398984 T3 ES 2398984T3 ES 08006778 T ES08006778 T ES 08006778T ES 08006778 T ES08006778 T ES 08006778T ES 2398984 T3 ES2398984 T3 ES 2398984T3
Authority
ES
Spain
Prior art keywords
pcr
temperature
sample
stage
seconds
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES08006778T
Other languages
English (en)
Spanish (es)
Inventor
Waltraud Ankenbauer
Ursula Grepl
Rita Haerteis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Original Assignee
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG, Roche Diagnostics GmbH filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2398984T3 publication Critical patent/ES2398984T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES08006778T 2007-04-05 2008-04-03 RT-PCR rápida en una etapa Active ES2398984T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07007173 2007-04-05
EP07007173 2007-04-05

Publications (1)

Publication Number Publication Date
ES2398984T3 true ES2398984T3 (es) 2013-03-25

Family

ID=38453943

Family Applications (1)

Application Number Title Priority Date Filing Date
ES08006778T Active ES2398984T3 (es) 2007-04-05 2008-04-03 RT-PCR rápida en una etapa

Country Status (4)

Country Link
US (1) US8119353B2 (enExample)
EP (1) EP1978109B1 (enExample)
JP (1) JP5191041B2 (enExample)
ES (1) ES2398984T3 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9175332B2 (en) * 2010-07-29 2015-11-03 Roche Molecular Systems, Inc. Generic PCR
EP2625284B1 (en) 2010-10-04 2015-01-28 Roche Diagniostics GmbH Method for cell lysis in a rt-pcr reaction buffer
US20130171630A1 (en) * 2011-12-29 2013-07-04 QIAGEN Inc. Methods of using telomeres as markers for aging
SG10201810110RA (en) 2012-05-24 2018-12-28 Univ Utah Res Found Extreme pcr
JP7319646B2 (ja) * 2015-11-05 2023-08-02 ユニヴァーシティ・オヴ・ユタ・リサーチ・ファウンデイション エクストリーム逆転写ｐｃｒ
JP2018023360A (ja) * 2016-07-29 2018-02-15 セイコーエプソン株式会社 核酸増幅反応方法、試薬、および試薬の使用方法
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
CN113795593A (zh) * 2019-01-17 2021-12-14 西北大学 快速逆转录定量聚合酶链式反应
EP4269592A4 (en) 2020-12-25 2025-01-01 Nippon Gene Co., Ltd MODIFIED DNA POLYMERASE

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5105599A (en) * 1989-02-24 1992-04-21 Highland Supply Corporation Means for securing a decorative cover about a flower pot
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5455170A (en) * 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5561058A (en) * 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
ES2100945T3 (es) 1989-12-22 1997-07-01 Hoffmann La Roche Transcriptasas reversas de alta temperatura.
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
AU5142393A (en) 1992-09-29 1994-04-26 Cedars-Sinai Medical Center One step rna and combined one step rna and dna polymerase chain reaction for detection of rare rna or rna and dna
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
US6174670B1 (en) * 1996-06-04 2001-01-16 University Of Utah Research Foundation Monitoring amplification of DNA during PCR
WO1997046707A2 (en) 1996-06-04 1997-12-11 University Of Utah Research Foundation System and method for monitoring for dna amplification by fluorescence
US7179590B2 (en) * 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
CA2439402A1 (en) * 2001-03-02 2002-09-12 University Of Pittsburgh Of The Commonwealth System Of Higher Education Pcr method
US20030108898A1 (en) * 2001-07-03 2003-06-12 Paul Choppa Method for detection, quantitation and breakpoint cluster region determination of fusion transcripts
CA2513292C (en) * 2003-01-17 2016-04-05 The Chinese University Of Hong Kong Circulating mrna as diagnostic markers for pregnancy-related disorders

Also Published As

Publication number Publication date
JP2008253263A (ja) 2008-10-23
EP1978109A1 (en) 2008-10-08
JP5191041B2 (ja) 2013-04-24
US20080248535A1 (en) 2008-10-09
EP1978109B1 (en) 2012-12-05
US8119353B2 (en) 2012-02-21

Similar Documents

Publication Publication Date Title
ES2398984T3 (es) RT-PCR rápida en una etapa
ES2880335T3 (es) Métodos y composiciones para la preparación rápida de bibliotecas de ácidos nucleicos
Kiddle et al. GMO detection using a bioluminescent real time reporter (BART) of loop mediated isothermal amplification (LAMP) suitable for field use
ES2759872T3 (es) Reacción de amplificación de extensión y mellado para la amplificación exponencial de los ácidos nucleicos
ES2523140T3 (es) Aumento de la confianza en las identificaciones de alelos con el recuento molecular
BR112020000809A2 (pt) uso da proteína cas, método para detecção de molécula de ácido nucleico, e kit
CA2730761C (en) Improved lysis and reverse transcription for mrna quantification
CN107446919B (zh) 一种恒温条件下合成核酸的方法及试剂盒
KR20120084320A (ko) Ｔｈｄ 프라이머 타겟 검출
CN114507716B (zh) 检测样品中靶核酸的方法
ES2685710T3 (es) Kits que comprenden controles para amplificación de ácido nucleico
CN101680040A (zh) 改良的诺如病毒rna检测方法
CN107922969A (zh) 包含核酸组装体的结构切换、核酸消化和扩增的串联反应的生物传感器
US7919244B2 (en) Nucleic acid detection method involving the direct generation of a measurable signal
Anbazhagan et al. Advances in plant pathogen detection: integrating recombinase polymerase amplification with CRISPR/Cas systems
US20210102237A1 (en) Means and methods for nucleic acid target detection
ES2350525T3 (es) Nuevo formato de detección para las reacciones en cadena de la polimerasa en tiempo real de inicio en caliente.
WO2019051732A1 (zh) 一种恒温条件下合成核酸的方法及试剂盒
JP2020530434A (ja) Ｒｎａおよびｄｎａからの核酸ライブラリーの作製
JP5887078B2 (ja) 合成ｓｉＲＮＡ検出方法
JP7160142B2 (ja) 核酸増幅用試薬及び核酸増幅法
ES3015628T3 (en) Method for amplifying nucleotide sequence, and sequence determination method
Qiao et al. A split crRNA with CRISPR-Cas12a enables highly sensitive, selective, and multiplexed detection of RNA and DNA
US9157128B2 (en) Kit for detecting HIV-2 and method for detecting HIV-2 using the same
JP2019198259A (ja) １ステップｒｔ−ｐｃｒ法