JP5191041B2 - 急速ワンステップｒｔ−ｐｃｒ - Google Patents

急速ワンステップｒｔ−ｐｃｒ Download PDF

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Publication number
JP5191041B2
JP5191041B2 JP2008094907A JP2008094907A JP5191041B2 JP 5191041 B2 JP5191041 B2 JP 5191041B2 JP 2008094907 A JP2008094907 A JP 2008094907A JP 2008094907 A JP2008094907 A JP 2008094907A JP 5191041 B2 JP5191041 B2 JP 5191041B2
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JP
Japan
Prior art keywords
pcr
temperature
polymerase
seconds
reverse transcriptase
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JP2008094907A
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English (en)
Japanese (ja)
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JP2008253263A (ja
JP2008253263A5 (enExample
Inventor
アンケンバウアー ヴァルトラウト
グレープル ウルズラ
ヘールタイス リータ
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of JP2008253263A publication Critical patent/JP2008253263A/ja
Publication of JP2008253263A5 publication Critical patent/JP2008253263A5/ja
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2008094907A 2007-04-05 2008-04-01 急速ワンステップｒｔ−ｐｃｒ Active JP5191041B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07007173.3 2007-04-05
EP07007173 2007-04-05

Publications (3)

Publication Number Publication Date
JP2008253263A JP2008253263A (ja) 2008-10-23
JP2008253263A5 JP2008253263A5 (enExample) 2010-04-08
JP5191041B2 true JP5191041B2 (ja) 2013-04-24

Family

ID=38453943

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2008094907A Active JP5191041B2 (ja) 2007-04-05 2008-04-01 急速ワンステップｒｔ−ｐｃｒ

Country Status (4)

Country Link
US (1) US8119353B2 (enExample)
EP (1) EP1978109B1 (enExample)
JP (1) JP5191041B2 (enExample)
ES (1) ES2398984T3 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022138887A1 (ja) 2020-12-25 2022-06-30 株式会社ニッポンジーン 改変型ｄｎａポリメラーゼ

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9175332B2 (en) * 2010-07-29 2015-11-03 Roche Molecular Systems, Inc. Generic PCR
EP2625284B1 (en) 2010-10-04 2015-01-28 Roche Diagniostics GmbH Method for cell lysis in a rt-pcr reaction buffer
US20130171630A1 (en) * 2011-12-29 2013-07-04 QIAGEN Inc. Methods of using telomeres as markers for aging
SG10201810110RA (en) 2012-05-24 2018-12-28 Univ Utah Res Found Extreme pcr
JP7319646B2 (ja) * 2015-11-05 2023-08-02 ユニヴァーシティ・オヴ・ユタ・リサーチ・ファウンデイション エクストリーム逆転写ｐｃｒ
JP2018023360A (ja) * 2016-07-29 2018-02-15 セイコーエプソン株式会社 核酸増幅反応方法、試薬、および試薬の使用方法
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
CN113795593A (zh) * 2019-01-17 2021-12-14 西北大学 快速逆转录定量聚合酶链式反应

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5105599A (en) * 1989-02-24 1992-04-21 Highland Supply Corporation Means for securing a decorative cover about a flower pot
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5455170A (en) * 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5561058A (en) * 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
ES2100945T3 (es) 1989-12-22 1997-07-01 Hoffmann La Roche Transcriptasas reversas de alta temperatura.
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
AU5142393A (en) 1992-09-29 1994-04-26 Cedars-Sinai Medical Center One step rna and combined one step rna and dna polymerase chain reaction for detection of rare rna or rna and dna
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
US6174670B1 (en) * 1996-06-04 2001-01-16 University Of Utah Research Foundation Monitoring amplification of DNA during PCR
WO1997046707A2 (en) 1996-06-04 1997-12-11 University Of Utah Research Foundation System and method for monitoring for dna amplification by fluorescence
US7179590B2 (en) * 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
CA2439402A1 (en) * 2001-03-02 2002-09-12 University Of Pittsburgh Of The Commonwealth System Of Higher Education Pcr method
US20030108898A1 (en) * 2001-07-03 2003-06-12 Paul Choppa Method for detection, quantitation and breakpoint cluster region determination of fusion transcripts
CA2513292C (en) * 2003-01-17 2016-04-05 The Chinese University Of Hong Kong Circulating mrna as diagnostic markers for pregnancy-related disorders

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022138887A1 (ja) 2020-12-25 2022-06-30 株式会社ニッポンジーン 改変型ｄｎａポリメラーゼ

Also Published As

Publication number Publication date
JP2008253263A (ja) 2008-10-23
ES2398984T3 (es) 2013-03-25
EP1978109A1 (en) 2008-10-08
US20080248535A1 (en) 2008-10-09
EP1978109B1 (en) 2012-12-05
US8119353B2 (en) 2012-02-21

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