ES2374403T3 - Cuantificación de anticuerpos. - Google Patents
Cuantificación de anticuerpos. Download PDFInfo
- Publication number
- ES2374403T3 ES2374403T3 ES07865898T ES07865898T ES2374403T3 ES 2374403 T3 ES2374403 T3 ES 2374403T3 ES 07865898 T ES07865898 T ES 07865898T ES 07865898 T ES07865898 T ES 07865898T ES 2374403 T3 ES2374403 T3 ES 2374403T3
- Authority
- ES
- Spain
- Prior art keywords
- antibody
- interest
- peptides
- sample
- fragments
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000011002 quantification Methods 0.000 title description 16
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 107
- 238000000034 method Methods 0.000 claims abstract description 50
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 47
- 108090000284 Pepsin A Proteins 0.000 claims abstract description 41
- 102000057297 Pepsin A Human genes 0.000 claims abstract description 41
- 229940111202 pepsin Drugs 0.000 claims abstract description 39
- 239000012634 fragment Substances 0.000 claims abstract description 31
- 239000012472 biological sample Substances 0.000 claims abstract description 27
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 27
- 230000029087 digestion Effects 0.000 claims abstract description 26
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 26
- 239000000523 sample Substances 0.000 claims description 56
- 210000002966 serum Anatomy 0.000 claims description 36
- 238000004458 analytical method Methods 0.000 claims description 24
- 108091005804 Peptidases Proteins 0.000 claims description 23
- 239000004365 Protease Substances 0.000 claims description 23
- 102000035195 Peptidases Human genes 0.000 claims description 19
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims description 17
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims description 17
- 238000004949 mass spectrometry Methods 0.000 claims description 11
- 238000000926 separation method Methods 0.000 claims description 11
- 230000000875 corresponding effect Effects 0.000 claims description 10
- 108060003951 Immunoglobulin Proteins 0.000 claims description 9
- 102000018358 immunoglobulin Human genes 0.000 claims description 9
- 150000002500 ions Chemical class 0.000 claims description 9
- 241000894007 species Species 0.000 claims description 4
- 125000000539 amino acid group Chemical group 0.000 claims description 3
- 230000009849 deactivation Effects 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 230000017854 proteolysis Effects 0.000 claims description 3
- 238000004885 tandem mass spectrometry Methods 0.000 claims description 3
- 108010088751 Albumins Proteins 0.000 claims description 2
- 102000009027 Albumins Human genes 0.000 claims description 2
- 238000013375 chromatographic separation Methods 0.000 claims description 2
- 230000002596 correlated effect Effects 0.000 claims description 2
- 238000000338 in vitro Methods 0.000 claims description 2
- 238000010606 normalization Methods 0.000 claims description 2
- 230000001225 therapeutic effect Effects 0.000 claims description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 4
- 241000124008 Mammalia Species 0.000 claims 1
- 238000000589 high-performance liquid chromatography-mass spectrometry Methods 0.000 abstract description 15
- 238000001514 detection method Methods 0.000 abstract description 5
- 238000011992 High performance liquid chromatography with tandem mass spectrometry Methods 0.000 abstract description 2
- 235000018102 proteins Nutrition 0.000 description 21
- 239000000203 mixture Substances 0.000 description 10
- 238000002965 ELISA Methods 0.000 description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 241000700159 Rattus Species 0.000 description 9
- 235000019419 proteases Nutrition 0.000 description 9
- 108090000631 Trypsin Proteins 0.000 description 8
- 102000004142 Trypsin Human genes 0.000 description 8
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 8
- 230000009467 reduction Effects 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 238000004128 high performance liquid chromatography Methods 0.000 description 7
- 239000011159 matrix material Substances 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- 239000012588 trypsin Substances 0.000 description 7
- 238000010200 validation analysis Methods 0.000 description 7
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
- 230000027455 binding Effects 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 238000003556 assay Methods 0.000 description 5
- 238000011088 calibration curve Methods 0.000 description 5
- 229940088598 enzyme Drugs 0.000 description 5
- 238000002414 normal-phase solid-phase extraction Methods 0.000 description 5
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 4
- 239000000427 antigen Substances 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 235000019253 formic acid Nutrition 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 230000007704 transition Effects 0.000 description 3
- 239000004475 Arginine Substances 0.000 description 2
- YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical compound [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 description 2
- 108010051815 Glutamyl endopeptidase Proteins 0.000 description 2
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 2
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 2
- 108010067060 Immunoglobulin Variable Region Proteins 0.000 description 2
- 102000017727 Immunoglobulin Variable Region Human genes 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 2
- 239000012491 analyte Substances 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 239000013592 cell lysate Substances 0.000 description 2
- 150000001793 charged compounds Chemical class 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000001360 collision-induced dissociation Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 229910052805 deuterium Inorganic materials 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000002198 insoluble material Substances 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 229930182817 methionine Natural products 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000013207 serial dilution Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 1
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 1
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 108010004032 Bromelains Proteins 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- YTRPTVLTUWVLKO-ZDUSSCGKSA-N Ficine Natural products CN1CCC[C@H]1C1=C(O)C=C(O)C2=C1OC(C=1C=CC=CC=1)=CC2=O YTRPTVLTUWVLKO-ZDUSSCGKSA-N 0.000 description 1
- 239000007987 MES buffer Substances 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 108010067372 Pancreatic elastase Proteins 0.000 description 1
- 102000016387 Pancreatic elastase Human genes 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 102000012479 Serine Proteases Human genes 0.000 description 1
- 108010022999 Serine Proteases Proteins 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 108010006886 Vitrogen Proteins 0.000 description 1
- 108010046377 Whey Proteins Proteins 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 1
- 239000001099 ammonium carbonate Substances 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- 239000000611 antibody drug conjugate Substances 0.000 description 1
- 229940049595 antibody-drug conjugate Drugs 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 235000019835 bromelain Nutrition 0.000 description 1
- 238000004422 calculation algorithm Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 230000000779 depleting effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- YTRPTVLTUWVLKO-UHFFFAOYSA-N ficine Chemical compound CN1CCCC1C1=C(O)C=C(O)C2=C1OC(C=1C=CC=CC=1)=CC2=O YTRPTVLTUWVLKO-UHFFFAOYSA-N 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000000126 in silico method Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 238000005040 ion trap Methods 0.000 description 1
- 230000000155 isotopic effect Effects 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 231100000324 minimal toxicity Toxicity 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 230000007026 protein scission Effects 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 238000011272 standard treatment Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000002110 toxicologic effect Effects 0.000 description 1
- 231100000759 toxicological effect Toxicity 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 125000002987 valine group Chemical class [H]N([H])C([H])(C(*)=O)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 230000002747 voluntary effect Effects 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 235000021119 whey protein Nutrition 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
- G01N33/6857—Antibody fragments
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Biophysics (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US87137806P | 2006-12-21 | 2006-12-21 | |
| US871378P | 2006-12-21 | ||
| PCT/US2007/088258 WO2008079914A1 (en) | 2006-12-21 | 2007-12-20 | Antibody quantitation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2374403T3 true ES2374403T3 (es) | 2012-02-16 |
Family
ID=39247371
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES07865898T Active ES2374403T3 (es) | 2006-12-21 | 2007-12-20 | Cuantificación de anticuerpos. |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20100015652A1 (de) |
| EP (1) | EP2097756B1 (de) |
| JP (1) | JP2010515020A (de) |
| AT (1) | ATE529752T1 (de) |
| ES (1) | ES2374403T3 (de) |
| WO (1) | WO2008079914A1 (de) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2436744T3 (es) * | 2007-08-24 | 2014-01-07 | Emd Millipore Corporation | Sistema para derivar un eluyente de cromatografía de líquidos |
| EP2181326A2 (de) | 2007-08-24 | 2010-05-05 | Millipore Corporation | Verfahren zur erkennung abbaubarer filterelemente in biopharmazeutischen produkten durch flüssigchromatographische massenspektrometrie |
| WO2010086386A1 (en) * | 2009-01-30 | 2010-08-05 | Pronota N.V. | Protein quantification methods and use thereof for candidate biomarker validation |
| US20120149022A1 (en) * | 2009-02-20 | 2012-06-14 | Eva I-Wei Aw | Compositions and methods for diagnosis and prognosis of colorectal cancer |
| CA2799746C (en) * | 2010-05-17 | 2020-11-24 | Sai Reddy | Rapid isolation of monoclonal antibodies from animals |
| AU2012225310B2 (en) | 2011-03-09 | 2017-08-03 | Cell Signaling Technology, Inc. | Methods and reagents for creating monoclonal antibodies |
| EP2707723B1 (de) | 2011-05-12 | 2016-02-10 | Genentech, Inc. | Lc-ms/ms-verfahren mit mehrfachreaktionsüberwachung zur erkennung therapeutischer antikörper in tierischen proben mittels rahmensignaturpeptiden |
| GB201110371D0 (en) * | 2011-06-17 | 2011-08-03 | Proteome Sciences R & D Gmbh & Co Kg | Materials and methods for determining sensitivity potential of compounds |
| EP2783214A2 (de) | 2011-11-23 | 2014-10-01 | The Board of Regents of The University of Texas System | Proteomischer nachweis von antikörpern |
| JP6558830B2 (ja) | 2012-06-15 | 2019-08-14 | ボード・オブ・リージエンツ，ザ・ユニバーシテイ・オブ・テキサス・システム | 複数転写産物のハイスループットシークエンシング |
| PT3387898T (pt) | 2013-03-15 | 2020-06-17 | Mayo Found Medical Education & Res | Identificação e monitorização de imunoglobulinas monoclonais pela massa molecular |
| CN105518447B (zh) | 2013-09-09 | 2018-01-16 | 株式会社岛津制作所 | 肽片段的制备方法及该方法中使用的肽片段制备用试剂盒、以及分析方法 |
| JP6742235B2 (ja) * | 2014-02-18 | 2020-08-19 | 株式会社島津製作所 | 質量分析を用いたタンパク質の検出方法 |
| JP6759104B2 (ja) | 2014-04-04 | 2020-09-23 | メイヨ・ファウンデーション・フォー・メディカル・エデュケーション・アンド・リサーチ | 精密分子質量を用いた免疫グロブリンのアイソタイピング |
| BR112017017854A2 (pt) | 2015-03-09 | 2018-04-10 | Shimadzu Corporation | método para detecção de anticorpo monoclonal utilizando espectrometria de massa |
| WO2016143227A1 (ja) * | 2015-03-10 | 2016-09-15 | 株式会社島津製作所 | モノクローナル抗体の検出のためのサンプル調製用キット |
| US10513733B2 (en) | 2015-03-23 | 2019-12-24 | Board Of Regents, The University Of Texas System | High throughout sequencing of paired VH and VL transcripts from B cells secreting antigen-specific antibodies |
| JP6968058B2 (ja) | 2015-09-24 | 2021-11-17 | メイヨ・ファウンデーション・フォー・メディカル・エデュケーション・アンド・リサーチ | 質量分析法による免疫グロブリン遊離軽鎖の同定 |
| GB201603291D0 (en) * | 2016-02-25 | 2016-04-13 | Binding Site Group The Ltd | Antibodies |
| CN108700598A (zh) | 2016-03-25 | 2018-10-23 | 豪夫迈·罗氏有限公司 | 多路总抗体和抗体缀合的药物量化测定法 |
| CN109313200B (zh) | 2016-05-27 | 2022-10-04 | 豪夫迈·罗氏有限公司 | 用于表征位点特异性抗体-药物缀合物的生物分析性方法 |
| AU2017312785B2 (en) * | 2016-08-16 | 2024-10-10 | Regeneron Pharmaceuticals, Inc. | Methods for quantitating individual antibodies from a mixture |
| US10955420B2 (en) | 2016-09-07 | 2021-03-23 | Mayo Foundation For Medical Education And Research | Identification and monitoring of cleaved immunoglobulins by molecular mass |
| WO2019055632A1 (en) | 2017-09-13 | 2019-03-21 | Mayo Foundation For Medical Education And Research | IDENTIFICATION AND MONITORING OF IMMUNOGLOBULIN CHAINS J |
| EP3681528B1 (de) | 2017-09-13 | 2025-07-23 | Mayo Foundation for Medical Education and Research | Identifizierung und überwachung des apoptosehemmers von makrophagen |
| WO2019084538A1 (en) | 2017-10-27 | 2019-05-02 | Board Of Regents, The University Of Texas System | TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF |
| GB2607739B (en) * | 2018-06-06 | 2023-04-05 | Bruker Daltonics Gmbh & Co Kg | Targeted protein characterization by mass spectrometry |
| CA3179460A1 (en) * | 2020-06-09 | 2021-12-16 | Regeneron Pharmaceuticals, Inc. | A high-throughput and mass-spectrometry-based method for quantitating antibodies |
| CN117581103A (zh) * | 2021-06-23 | 2024-02-20 | 株式会社岛津制作所 | 分析抗体的方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3491748B2 (ja) * | 1999-07-22 | 2004-01-26 | 株式会社いかがく | 血液中の低比重リポ蛋白（ｌｄｌ）もしくは変性低比重リポ蛋白の検出方法 |
| JP4604345B2 (ja) * | 2000-12-21 | 2011-01-05 | 住友化学株式会社 | タンパク質のｎ末端のアミノ酸配列決定方法 |
| EP1457499A1 (de) * | 2003-03-12 | 2004-09-15 | Tufts University School Of Medicine | Inhibitoren des extrazellulären Hitzeschockproteins Hsp90 |
| CA2559870A1 (en) * | 2004-04-07 | 2005-10-27 | Genetech,Inc. | Mass spectrometry of antibody conjugates |
-
2007
- 2007-12-20 WO PCT/US2007/088258 patent/WO2008079914A1/en not_active Ceased
- 2007-12-20 JP JP2009543205A patent/JP2010515020A/ja active Pending
- 2007-12-20 AT AT07865898T patent/ATE529752T1/de not_active IP Right Cessation
- 2007-12-20 EP EP07865898A patent/EP2097756B1/de not_active Not-in-force
- 2007-12-20 US US12/520,031 patent/US20100015652A1/en not_active Abandoned
- 2007-12-20 ES ES07865898T patent/ES2374403T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20100015652A1 (en) | 2010-01-21 |
| EP2097756A1 (de) | 2009-09-09 |
| WO2008079914A1 (en) | 2008-07-03 |
| JP2010515020A (ja) | 2010-05-06 |
| ATE529752T1 (de) | 2011-11-15 |
| EP2097756B1 (de) | 2011-10-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2097756B1 (de) | Antikörperquantifizierung | |
| US8227251B2 (en) | Mass spectrum-based identification and quantitation of proteins and peptides | |
| US10281473B2 (en) | Compositions and methods for analysis of protein sequences and post-translational modifications | |
| US10175242B2 (en) | Personalized myeloma detection | |
| ES2409349T3 (es) | Método de cuantificación de un péptido y una proteína | |
| JP2013507603A (ja) | 署名ペプチドおよび質量分析による混合物中の個々の組換えタンパク質の多重定量化 | |
| ES2815224T3 (es) | Un método para cuantificar anticuerpos terapéuticos | |
| Jansson | Strategies for analysis of isomeric peptides | |
| US7115378B2 (en) | Peptide biomarker and method of identification | |
| US20110111424A1 (en) | Analysis of ubiquitinated polypeptides | |
| EP3165928B1 (de) | Verfahren zur quantifizierung von anti-tnf-antikörpern | |
| Pham et al. | De novo proteomic sequencing of a monoclonal antibody raised against OX40 ligand | |
| Zhang et al. | Evaluation of sample preparation methods for label-free quantitative profiling of salivary proteome | |
| Timm et al. | Identification and characterization of oxidation and deamidation sites in monoclonal rat/mouse hybrid antibodies | |
| Mhike et al. | Characterization of methylene diphenyl diisocyanate-haptenated human serum albumin and hemoglobin | |
| US7244411B2 (en) | Method of selective peptide isolation for the identification and quantitative analysis of proteins in complex mixtures | |
| US11352418B2 (en) | Analysis of ubiquitinated polypeptides | |
| Chen et al. | Sequential immunoaffinity-LC/MS assay for quantitation of a therapeutic protein in monkey plasma | |
| Gould et al. | LC-MS/MS method for quantifying the HIV-1 broadly neutralizing antibody PGT 121.414. LS in human serum | |
| CN116235055A (zh) | 作为神经退行性疾病的诊断和预后的标志物的p53翻译后修饰 | |
| Reuter et al. | Phosphonylated tyrosine and cysteine disulfide adducts both generated from immunoglobulin G and human serum albumin indicate exposure to the nerve agent VX in vitro: H. Reuter et al. | |
| EP3371602B1 (de) | Verfahren zur quantifizierung von anti-tnf-antikörpern | |
| Jiang et al. | Quantification of protein posttranslational modifications using stable isotope and mass spectrometry: I: Principles and applications | |
| ES3034592T3 (en) | Use of lc-ms/ms to quantitate protein biomarkers | |
| KR20170071849A (ko) | Lc-ms를 이용하는 항체 약물의 혈액 내 정량방법 |