ES2317706T3 - Metodo para produicir polipeptidos en celulas mutantes de aspergillus. - Google Patents

Metodo para produicir polipeptidos en celulas mutantes de aspergillus. Download PDF

Info

Publication number
ES2317706T3
ES2317706T3 ES99960956T ES99960956T ES2317706T3 ES 2317706 T3 ES2317706 T3 ES 2317706T3 ES 99960956 T ES99960956 T ES 99960956T ES 99960956 T ES99960956 T ES 99960956T ES 2317706 T3 ES2317706 T3 ES 2317706T3
Authority
ES
Spain
Prior art keywords
aspergillus
toxin
mutant
polypeptide
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES99960956T
Other languages
English (en)
Spanish (es)
Inventor
Bjorn Eggert Christensen
Henrik Mollgaard
Svend Kaasgaard
Jan Lehmbeck
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Application granted granted Critical
Publication of ES2317706T3 publication Critical patent/ES2317706T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungi isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/66—Aspergillus
    • C12R2001/69—Aspergillus oryzae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
ES99960956T 1998-12-23 1999-12-22 Metodo para produicir polipeptidos en celulas mutantes de aspergillus. Expired - Lifetime ES2317706T3 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DK199801726 1998-12-23
DKPA199801726 1998-12-23
DK199900745 1999-05-27
DKPA199900745 1999-05-27

Publications (1)

Publication Number Publication Date
ES2317706T3 true ES2317706T3 (es) 2009-04-16

Family

ID=26064565

Family Applications (1)

Application Number Title Priority Date Filing Date
ES99960956T Expired - Lifetime ES2317706T3 (es) 1998-12-23 1999-12-22 Metodo para produicir polipeptidos en celulas mutantes de aspergillus.

Country Status (9)

Country Link
EP (1) EP1141371B1 (https=)
JP (2) JP2002533133A (https=)
CN (1) CN100529096C (https=)
AT (1) ATE414782T1 (https=)
AU (1) AU1774000A (https=)
DE (1) DE69939947D1 (https=)
DK (1) DK1141371T3 (https=)
ES (1) ES2317706T3 (https=)
WO (1) WO2000039322A1 (https=)

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003206684A1 (en) * 2002-02-20 2003-09-09 Novozymes A/S Plant polypeptide production
US7396670B2 (en) 2002-10-11 2008-07-08 Novozymes A/S Asparaginases and method of preparing a heat-treated product
WO2004090549A1 (en) * 2003-04-11 2004-10-21 Novozymes A/S Method of screening for secreted glycosylated polypeptides
DK1620551T3 (da) 2003-04-28 2014-01-13 Chr Hansen As Phospholipase og fremgangsmåde til fremstilling deraf
ATE516347T1 (de) 2003-10-23 2011-07-15 Novozymes As Protease mit verbesserter stabilität in detergentien
WO2006053565A2 (en) 2004-11-19 2006-05-26 Novozymes A/S Polypeptides having antimicrobial activity and polynucleotides encoding same
EP1831384B1 (en) 2004-12-22 2015-08-12 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
US7888489B2 (en) 2005-01-24 2011-02-15 Dsm Ip Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
EP1739428A1 (en) * 2005-07-01 2007-01-03 Institut Pasteur In vitro diagnostic method and kit for an aspergillus infection
EP1941025B1 (en) 2005-10-17 2011-06-29 Novozymes A/S Use of fungal mutants for expression of antibodies
WO2008000632A1 (en) 2006-06-29 2008-01-03 Dsm Ip Assets B.V. A method for achieving improved polypeptide expression
EP2059590B1 (en) 2006-07-14 2014-12-31 Novozymes, Inc. Methods for producing secreted polypeptides having biological activity
WO2009051152A1 (ja) * 2007-10-19 2009-04-23 Noda Institute For Scientific Research ポリケタイドシンターゼ・ノンリボゾーマルペプチドシンテターゼ遺伝子
EP2857515B1 (en) 2008-11-20 2018-02-21 Novozymes Inc. Polypeptides having amylolytic enhancing activity and polynucleotides encoding same
AU2010206181B2 (en) 2009-01-21 2015-01-15 Novozymes A/S Polypeptides having esterase activity and nucleic acids encoding the same
WO2010108918A1 (en) 2009-03-24 2010-09-30 Novozymes A/S Polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
EP2421986A1 (en) 2009-04-22 2012-02-29 DSM IP Assets B.V. Process for the production of a recombinant polypeptide of interest
CA2758396C (en) 2009-04-24 2018-06-12 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
EP3293264A1 (en) 2009-07-22 2018-03-14 DSM IP Assets B.V. Improved host cell for the production of a compound of interest
US8586829B2 (en) 2009-09-30 2013-11-19 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2011057083A1 (en) 2009-11-06 2011-05-12 Novozymes, Inc. Polypeptides having xylanase activity and polynucleotides encoding same
EP2496694B1 (en) 2009-11-06 2017-04-19 Novozymes, Inc. Compositions for saccharification of cellulosic material
US9267125B2 (en) 2009-11-06 2016-02-23 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US9133448B2 (en) 2010-02-11 2015-09-15 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
EA201201125A1 (ru) 2010-02-11 2013-03-29 ДСМ АйПи АССЕТС Б.В. Клетка-хозяин, способная продуцировать ферменты, пригодные для деградации лигноцеллюлозного материала
WO2011161208A1 (en) 2010-06-25 2011-12-29 Novozymes A/S Polynucleotides having promoter activity
CN103068988A (zh) 2010-06-25 2013-04-24 诺维信公司 具有启动子活性的多核苷酸
CN103068978B (zh) 2010-06-25 2017-01-18 诺维信公司 具有前导序列功能的多核苷酸
DK2588603T3 (en) 2010-06-29 2017-07-03 Dsm Ip Assets Bv POLYPEPTIDE THAT HAS OR CONTRIBUTES TO CARBOHYDRATE MATERIAL DEGRADING ACTIVITIES AND APPLICATIONS THEREOF
EP2588493A1 (en) 2010-06-29 2013-05-08 DSM IP Assets B.V. Polypeptide having swollenin activity and uses thereof
ES2672125T3 (es) 2010-06-29 2018-06-12 Dsm Ip Assets B.V. Polipéptido que tiene actividad beta-glucosidasa y usos del mismo
EP2588601A1 (en) 2010-06-29 2013-05-08 DSM IP Assets B.V. Polypeptide having carbohydrate degrading activity and uses thereof
MX2012015144A (es) 2010-06-29 2013-03-05 Dsm Ip Assets Bv Polipeptido que tiene actividad de acetil-xilan-esterasa y usos del mismo.
WO2012000886A1 (en) 2010-06-29 2012-01-05 Dsm Ip Assets B.V. Polypeptide having beta-glucosidase activity and uses thereof
EP2588604B1 (en) 2010-06-30 2016-06-29 Novozymes, Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
AU2011273351A1 (en) 2010-07-01 2013-01-17 Dsm Ip Assets B.V. A method for the production of a compound of interest
BR112013002979A2 (pt) 2010-08-12 2016-06-07 Novozymes Inc composição, e, método para degradar ou converter um material celulósico, para produzir um produto de fermentação, e para fermentar um material celulósico.
US9187742B2 (en) 2010-08-30 2015-11-17 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US9267126B2 (en) 2010-08-30 2016-02-23 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
BR112013009026A2 (pt) 2010-11-12 2016-07-12 Novozymes Inc polipeptídeo isolado, célula hospedeira recombinante, métodos para produzir um polipeptídeo, um mutante de uma célula precursora, uma proteína, e um produto de fermentação, para inibir a expressão de um polipeptídeo, para degradar ou converter um material celulósico, e método para fermentar um material celulósico, planta transgênica, parte de planta ou célula de planta, molécula de rna inibidora de filamento duplo, polinucleotídeo isolado, e, formulação de caldo integral ou composição de cultura de célula
WO2012103322A1 (en) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
EP2668269B1 (en) 2011-01-26 2018-01-03 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
MX2013007720A (es) 2011-01-26 2013-08-09 Novozymes As Polipeptidos que tienen actividad celobiohidrolasa y polinucleotidos que codifican para los mismos.
EP2668270B1 (en) 2011-01-26 2018-11-21 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US8759023B2 (en) 2011-01-26 2014-06-24 Novozymes Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
CN103608461B (zh) 2011-03-09 2016-08-03 诺维信公司 增加多肽的纤维素分解增强活性的方法
EP2702153B1 (en) 2011-04-28 2018-12-12 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
DK2702162T3 (da) 2011-04-29 2020-05-18 Novozymes Inc Fremgangsmåder til forbedring af nedbrydningen eller omdannelsen af celluloseholdigt materiale
EP2527448A1 (en) 2011-05-23 2012-11-28 Novozymes A/S Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi
CN102321700A (zh) * 2011-08-03 2012-01-18 无锡赛德生物工程有限公司 一种曲酸发酵工艺及发酵设备
US9663772B2 (en) 2011-08-24 2017-05-30 Novozymes, Inc. Aspergillus fumigatus cellulolytic enzyme compositions and uses thereof
WO2013064075A1 (en) 2011-10-31 2013-05-10 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US10351834B2 (en) 2011-11-21 2019-07-16 Novozymes, Inc. GH61 polypeptide variants and polynucleotides encoding same
US10227579B2 (en) 2012-04-27 2019-03-12 Novozymes A/S GH61 polypeptide variants and polynucleotides encoding same
AU2013311668B2 (en) 2012-09-05 2019-02-28 Novozymes A/S Polypeptides having protease activity
WO2014092832A2 (en) 2012-09-19 2014-06-19 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
EP3586610B1 (en) 2012-10-08 2024-09-25 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2014066141A2 (en) 2012-10-24 2014-05-01 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
BR112015013421A2 (pt) 2012-12-14 2017-11-14 Novozymes As E Novozymes Inc célula hospedeira microbiana transgênica, polipeptídeo isolado, métodos de produção de um polipeptídeo e de uma proteína, processos de degradação de um material celulósico, de produção de um produto de fermentação e de fermentação de um material celulósico, construção de ácido nucleico ou vetor de expressão, polipeptídeo isolado, e polinucleotídeo isolado
CN105164254B (zh) 2013-03-08 2019-06-28 诺维信公司 纤维二糖水解酶变体和编码它们的多核苷酸
DK2994529T3 (en) 2013-05-10 2019-03-04 Novozymes As Polypeptides with xylanase activity and polynucleotides encoding them
EP3594335B1 (en) 2014-09-05 2024-05-01 Novozymes A/S Carbohydrate binding module variants and polynucleotides encoding same
MX383142B (es) 2014-12-19 2025-03-13 Novozymes As Composiciones que comprenden polipéptidos que tienen actividad xilanasa y polipéptidos que tienen actividad arabinofuranosidasa.
US10557127B2 (en) 2015-02-24 2020-02-11 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
BR112017021489A2 (pt) 2015-05-27 2018-07-03 Novozymes A/S polipeptídeo tendo atividade de celobio-hidrolase, composição, formulação de caldo inteiro ou composição de cultura de células, polinucleotídeo, construto de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, métodos de produção do polipeptídeo.
US10479984B2 (en) 2015-09-22 2019-11-19 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US11242507B2 (en) * 2017-06-07 2022-02-08 Ptt Global Chemical Public Company Limited Mutant strain Aspergillus aculeatus for producing cellulase and xylanase and preparation method thereof
MY208100A (en) 2017-08-25 2025-04-15 Novozymes As Enzyme assisted crude palm oil extraction
DK3775222T5 (da) 2018-03-26 2024-08-19 Novozymes As Chaperoneproteiner fra svampe
CN108753748B (zh) * 2018-06-08 2021-12-10 中国中医科学院中药研究所 大黄素糖基转移酶蛋白FtUGT75R2及其编码基因与应用
CN108795903B (zh) * 2018-06-08 2020-12-15 南京林业大学 一种高酶活淀粉酶的表达方法
CN108823178B (zh) * 2018-06-08 2021-12-10 中国中医科学院中药研究所 大黄素糖基转移酶蛋白FtUGT73BE5及其编码基因与应用
BR112021011384A2 (pt) 2018-12-12 2022-05-17 Novozymes As Polipeptídeo isolado, composição de enzimas, formulação de caldo inteiro ou composição de cultura de células, polinucleotídeo isolado, célula hospedeira recombinante, método de produção de um polipeptídeo, planta, parte da planta ou célula da planta transgênica, e, processos para degradação de um material celulósico ou hemicelulósico, para produção de um produto de fermentação e de fermentação de um material celulósico ou hemicelulósico
CN113316641B (zh) 2018-12-21 2025-01-28 诺维信公司 串联蛋白表达
CN110218750B (zh) * 2019-04-24 2023-04-11 郑州轻工业学院 杂色曲霉菌株在发酵生产曲酸中应用
CN110108874B (zh) * 2019-04-30 2022-09-20 中国农业科学院油料作物研究所 快速检测环匹阿尼酸的胶体金免疫分析试纸条、制备及其应用
CN110106152B (zh) * 2019-04-30 2021-08-17 中国农业科学院油料作物研究所 杂交瘤细胞株ytt-2及其产生的抗环匹阿尼酸单克隆抗体
CN114391038A (zh) 2019-07-25 2022-04-22 诺维信公司 丝状真菌表达系统
CN111139189B (zh) * 2020-01-14 2022-04-19 浙江工业大学 曲霉wbx-38及其在生产环匹阿尼酸中的应用
CN116024096B (zh) * 2022-07-14 2025-06-03 浙江树人学院 泡盛曲霉sr3-28及其在从朱砂七提取大黄素中的应用
CN120476196A (zh) 2022-12-21 2025-08-12 诺维信公司 用于细胞分离的微生物蛋白酶
CN116064437B (zh) * 2023-02-20 2025-09-23 武汉轻工大学 用于降解黄曲霉毒素的复合酶制剂、复合酶制剂溶液及其制备方法和应用
WO2025133003A2 (en) 2023-12-19 2025-06-26 21St.Bio A/S Non-animal bovine beta lactoglobulin

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2178007A1 (en) * 1993-12-01 1995-06-08 Randy M. Berka Aspergillus expression system
CA2178008A1 (en) * 1993-12-01 1995-06-08 Randy M. Berka Aspergillus foetidus expression system
JP4436934B2 (ja) * 1996-09-13 2010-03-24 ノボザイムス，インコーポレイティド 変更された量のポリペプチドを生成するｄｎａ挿入突然変異を有する細胞
EP0956338B1 (en) * 1996-09-19 2005-12-21 Novozymes A/S Host cells and methods of producing proteins

Also Published As

Publication number Publication date
ATE414782T1 (de) 2008-12-15
JP2011188863A (ja) 2011-09-29
EP1141371A1 (en) 2001-10-10
DE69939947D1 (de) 2009-01-02
CN100529096C (zh) 2009-08-19
JP5453343B2 (ja) 2014-03-26
DK1141371T3 (da) 2009-02-02
CN1333837A (zh) 2002-01-30
JP2002533133A (ja) 2002-10-08
AU1774000A (en) 2000-07-31
EP1141371B1 (en) 2008-11-19
WO2000039322A1 (en) 2000-07-06

Similar Documents

Publication Publication Date Title
ES2317706T3 (es) Metodo para produicir polipeptidos en celulas mutantes de aspergillus.
US5958727A (en) Methods for modifying the production of a polypeptide
KR100618495B1 (ko) 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서
US8945898B2 (en) Recombinant host cell with deficiency in non-ribosomal peptide synthase production
ES2538790T3 (es) Métodos para la producción de polipéptidos en mutantes de Fusarium venenatum deficientes en enzimas
US20080213901A1 (en) Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells
JP2012504390A (ja) 糸状菌細胞におけるポジティブ及びネガティブ選択遺伝子の使用方法
US12031168B2 (en) Methods for controlling protease production
US5989889A (en) Nucleic acids encoding polypeptides having tripeptide aminopeptidase activity
JP4490284B2 (ja) 菌類細胞において遺伝子を発現するためのプロモーター変異体
JP7609774B2 (ja) 修飾された糸状菌宿主細胞
US20180312849A1 (en) Methods For Producing Heterologous Polypeptides In Mutants Of Trichoderma
US6383781B1 (en) Methods for producing polypeptides in aspergillus mutant cells
JP2002515252A (ja) 糸状菌変異体細胞においてポリペプチドを生産するための方法
ES2296606T3 (es) Metodos de produccion de polipeptidos en celulas deficientes en ciclohexadepsipeptido.
CN113056552A (zh) 经修饰的丝状真菌宿主细胞
CN119584864A (zh) 磷脂在食品中的改善的酶促修饰
KR20030081371A (ko) 소르다리아시애 과의 자웅동체 진균에서 이형 단백질을생산하는 방법
JP2008125493A (ja) 染色体広範囲遺伝子領域を欠失した生物の作出法