CN100529096C - 在曲霉属突变细胞中产生多肽的方法 - Google Patents
在曲霉属突变细胞中产生多肽的方法 Download PDFInfo
- Publication number
- CN100529096C CN100529096C CNB99815766XA CN99815766A CN100529096C CN 100529096 C CN100529096 C CN 100529096C CN B99815766X A CNB99815766X A CN B99815766XA CN 99815766 A CN99815766 A CN 99815766A CN 100529096 C CN100529096 C CN 100529096C
- Authority
- CN
- China
- Prior art keywords
- aspergillus
- toxin
- cell
- mutant
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungi isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
- C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/66—Aspergillus
- C12R2001/69—Aspergillus oryzae
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA199801726 | 1998-12-23 | ||
| DKPA199801726 | 1998-12-23 | ||
| DKPA199900745 | 1999-05-27 | ||
| DKPA199900745 | 1999-05-27 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1333837A CN1333837A (zh) | 2002-01-30 |
| CN100529096C true CN100529096C (zh) | 2009-08-19 |
Family
ID=26064565
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB99815766XA Expired - Lifetime CN100529096C (zh) | 1998-12-23 | 1999-12-22 | 在曲霉属突变细胞中产生多肽的方法 |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP1141371B1 (https=) |
| JP (2) | JP2002533133A (https=) |
| CN (1) | CN100529096C (https=) |
| AT (1) | ATE414782T1 (https=) |
| AU (1) | AU1774000A (https=) |
| DE (1) | DE69939947D1 (https=) |
| DK (1) | DK1141371T3 (https=) |
| ES (1) | ES2317706T3 (https=) |
| WO (1) | WO2000039322A1 (https=) |
Families Citing this family (83)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2003206684A1 (en) * | 2002-02-20 | 2003-09-09 | Novozymes A/S | Plant polypeptide production |
| ES2533469T3 (es) | 2002-10-11 | 2015-04-10 | Novozymes A/S | Método de preparación de un producto tratado con calor |
| WO2004090549A1 (en) * | 2003-04-11 | 2004-10-21 | Novozymes A/S | Method of screening for secreted glycosylated polypeptides |
| EP1620551B1 (en) | 2003-04-28 | 2013-09-18 | Novozymes A/S | Phospholipase and method of producing it |
| WO2005040372A1 (en) | 2003-10-23 | 2005-05-06 | Novozymes A/S | Protease with improved stability in detergents |
| EP1814996A2 (en) | 2004-11-19 | 2007-08-08 | Novozymes A/S | Polypeptides having antimicrobial activity and polynucleotides encoding same |
| EP2365068B1 (en) | 2004-12-22 | 2017-03-01 | Novozymes A/S | Enzymes for starch processing |
| EP1841861A1 (en) | 2005-01-24 | 2007-10-10 | DSMIP Assets B.V. | Method for producing a compound of interest in a filamentous fungal cell |
| EP1739428A1 (en) * | 2005-07-01 | 2007-01-03 | Institut Pasteur | In vitro diagnostic method and kit for an aspergillus infection |
| WO2007045248A1 (en) | 2005-10-17 | 2007-04-26 | Novozymes A/S | Use of fungal mutants for expression of antibodies |
| DK2423315T3 (en) | 2006-06-29 | 2015-04-13 | Dsm Ip Assets Bv | A method for obtaining enhanced polypeptide expression |
| CN103451164A (zh) | 2006-07-14 | 2013-12-18 | 诺维信股份有限公司 | 用于产生具有生物学活性的分泌型多肽的方法 |
| JP5410981B2 (ja) * | 2007-10-19 | 2014-02-05 | 公益財団法人野田産業科学研究所 | ポリケタイドシンターゼ・ノンリボゾーマルペプチドシンテターゼ遺伝子 |
| CN102292444A (zh) | 2008-11-20 | 2011-12-21 | 诺维信股份有限公司 | 具有淀粉分解增强活性的多肽和编码该多肽的多核苷酸 |
| ES2558698T3 (es) | 2009-01-21 | 2016-02-08 | Novozymes A/S | Polipéptidos con actividad de esterasa y ácidos nucleicos que codifican los mismos |
| CA2752007A1 (en) | 2009-03-24 | 2010-09-30 | Novozymes A/S | Polypeptides having acetyl xylan esterase activity and polynucleotides encoding same |
| CA2758404A1 (en) | 2009-04-22 | 2010-10-28 | Dsm Ip Assets B.V. | Process for the production of a recombinant polypeptide of interest |
| US9163224B2 (en) | 2009-04-24 | 2015-10-20 | Dsm Ip Assets B.V. | Carbohydrate degrading polypeptide and uses thereof |
| WO2011009700A1 (en) | 2009-07-22 | 2011-01-27 | Dsm Ip Assets B.V. | Improved host cell for the production of a compound of interest |
| EP2483402A1 (en) | 2009-09-30 | 2012-08-08 | Novozymes A/S | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| CN104694517B (zh) | 2009-11-06 | 2019-06-28 | 诺维信股份有限公司 | 具有木聚糖酶活性的多肽和编码该多肽的多核苷酸 |
| US9267125B2 (en) | 2009-11-06 | 2016-02-23 | Novozymes, Inc. | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
| CN102947442B (zh) | 2009-11-06 | 2016-03-09 | 诺维信股份有限公司 | 用于糖化纤维素材料的组合物 |
| EA201201126A1 (ru) | 2010-02-11 | 2013-05-30 | ДСМ АйПи АССЕТС Б.В. | Полипептид, обладающий активностью целлобиогидролазы, и его применение |
| WO2011098577A1 (en) | 2010-02-11 | 2011-08-18 | Dsm Ip Assets B.V. | Host cell capable of producing enzymes useful for degradation of lignocellulosic material |
| EP2585601A1 (en) | 2010-06-25 | 2013-05-01 | Novozymes A/S | Polynucleotides having promoter activity |
| WO2011161206A1 (en) | 2010-06-25 | 2011-12-29 | Novozymes A/S | Polynucleotides having leader sequence function |
| EP2585602A1 (en) | 2010-06-25 | 2013-05-01 | Novozymes A/S | Polynucleotides having promoter activity |
| AU2011273689B2 (en) | 2010-06-29 | 2014-08-21 | Dsm Ip Assets B.V. | Polypeptide having carbohydrate degrading activity and uses thereof |
| MX2012014990A (es) | 2010-06-29 | 2013-02-26 | Dsm Ip Assets Bv | Polipeptido que tiene actividad suolenina y sus usos. |
| AU2011273690C1 (en) | 2010-06-29 | 2015-07-30 | Versalis S.P.A. | Polypeptide having or assisting in carbohydrate material degrading activity and uses thereof |
| EP2588494B1 (en) | 2010-06-29 | 2018-03-07 | DSM IP Assets B.V. | Polypeptide having beta-glucosidase activity and uses thereof |
| AU2011273686B2 (en) | 2010-06-29 | 2014-07-10 | Versalis S.P.A. | Polypeptide having acetyl xylan esterase activity and uses thereof |
| PL2588492T3 (pl) | 2010-06-29 | 2016-09-30 | Polipeptyd mający aktywność beta-glukozydazy i jego zastosowania | |
| EP2588604B1 (en) | 2010-06-30 | 2016-06-29 | Novozymes, Inc. | Polypeptides having beta-glucosidase activity and polynucleotides encoding same |
| CN103459600B (zh) * | 2010-07-01 | 2016-10-19 | 帝斯曼知识产权资产管理有限公司 | 用于生产感兴趣的化合物的方法 |
| US9394555B2 (en) | 2010-08-12 | 2016-07-19 | Novozymes, Inc. | Compositions comprising a polypeptide having cellulolytic enhancing activity and a dioxy compound and uses thereof |
| WO2012030844A1 (en) | 2010-08-30 | 2012-03-08 | Novozymes A/S | Polypeptides having endoglucanase activity and polynucleotides encoding same |
| US9187742B2 (en) | 2010-08-30 | 2015-11-17 | Novozymes, Inc. | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
| BR112013009026A2 (pt) | 2010-11-12 | 2016-07-12 | Novozymes Inc | polipeptídeo isolado, célula hospedeira recombinante, métodos para produzir um polipeptídeo, um mutante de uma célula precursora, uma proteína, e um produto de fermentação, para inibir a expressão de um polipeptídeo, para degradar ou converter um material celulósico, e método para fermentar um material celulósico, planta transgênica, parte de planta ou célula de planta, molécula de rna inibidora de filamento duplo, polinucleotídeo isolado, e, formulação de caldo integral ou composição de cultura de célula |
| US9353363B2 (en) | 2011-01-26 | 2016-05-31 | Novozymes A/S | Glycoside hydrolases from thermophilic fungi |
| WO2012103322A1 (en) | 2011-01-26 | 2012-08-02 | Novozymes A/S | Polypeptides having endoglucanase activity and polynucleotides encoding same |
| WO2012103350A1 (en) | 2011-01-26 | 2012-08-02 | Novozymes A/S | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
| CN103517986B (zh) | 2011-01-26 | 2016-12-07 | 诺维信公司 | 具有纤维二糖水解酶活性的多肽及编码该多肽的多核苷酸 |
| DK2668266T3 (en) | 2011-01-26 | 2018-03-26 | Novozymes Inc | POLYPEPTIDES WITH CELLOBIO HYDROASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM |
| US9150842B2 (en) | 2011-03-09 | 2015-10-06 | Novozymes A/S | Methods of increasing the cellulolytic enhancing activity of a polypeptide |
| CN103649308A (zh) | 2011-04-28 | 2014-03-19 | 诺维信股份有限公司 | 具有内切葡聚糖酶活性的多肽和编码该多肽的多核苷酸 |
| US9624518B2 (en) | 2011-04-29 | 2017-04-18 | Novozymes, Inc. | Methods for enhancing the degradation or conversion of cellulosic material |
| EP2527448A1 (en) | 2011-05-23 | 2012-11-28 | Novozymes A/S | Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi |
| CN102321700A (zh) * | 2011-08-03 | 2012-01-18 | 无锡赛德生物工程有限公司 | 一种曲酸发酵工艺及发酵设备 |
| CN103890169A (zh) | 2011-08-24 | 2014-06-25 | 诺维信股份有限公司 | 烟曲霉纤维素分解酶组合物及其用途 |
| DK2773656T3 (da) | 2011-10-31 | 2019-09-09 | Novozymes Inc | Polypeptider med cellulolyseforbedrende aktivitet og polynukleotider, som koder for dem |
| DK3219794T3 (da) | 2011-11-21 | 2019-12-16 | Novozymes Inc | GH61-polypeptidvarianter og polynukleotider, som koder for dem |
| CA2868308A1 (en) | 2012-04-27 | 2013-10-31 | Novozymes, Inc. | Gh61 polypeptide variants and polynucleotides encoding same |
| DK2893012T3 (en) | 2012-09-05 | 2018-12-03 | Novozymes As | Polypeptides with protease activity |
| CN104685052A (zh) | 2012-09-19 | 2015-06-03 | 诺维信股份有限公司 | 用于增强纤维素材料的降解或转化的方法 |
| US10035996B2 (en) | 2012-10-08 | 2018-07-31 | Novozymes, Inc. | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| US20150275194A1 (en) | 2012-10-24 | 2015-10-01 | Novozymes A/S | Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same |
| WO2014093835A1 (en) | 2012-12-14 | 2014-06-19 | Novozymes A/S | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| CN110628749A (zh) | 2013-03-08 | 2019-12-31 | 诺维信公司 | 纤维二糖水解酶变体和编码它们的多核苷酸 |
| CN105283546B (zh) | 2013-05-10 | 2025-08-12 | 国投生物科技投资有限公司 | 具有木聚糖酶活性的多肽以及对其进行编码的多核苷酸 |
| EP3594335B1 (en) | 2014-09-05 | 2024-05-01 | Novozymes A/S | Carbohydrate binding module variants and polynucleotides encoding same |
| MX383142B (es) | 2014-12-19 | 2025-03-13 | Novozymes As | Composiciones que comprenden polipéptidos que tienen actividad xilanasa y polipéptidos que tienen actividad arabinofuranosidasa. |
| DK3739045T3 (da) | 2015-02-24 | 2024-10-21 | Novozymes As | Cellobiohydrolasevarianter og polynukleotider, som koder for dem |
| CN116676293A (zh) | 2015-05-27 | 2023-09-01 | 国投生物科技投资有限公司 | 具有纤维二糖水解酶活性的多肽以及对其进行编码的多核苷酸 |
| CN108350044B (zh) | 2015-09-22 | 2022-05-24 | 诺维信公司 | 具有纤维二糖水解酶活性的多肽以及对其进行编码的多核苷酸 |
| JP6950873B2 (ja) * | 2017-06-07 | 2021-10-13 | ピーティーティー グローバル ケミカル パブリック カンパニー リミテッド | セルラーゼ及びキシラナーゼを生産するための変異株アスペルギルス・アクレアツス並びにその調製方法 |
| AU2018319349B2 (en) | 2017-08-25 | 2024-02-29 | Novozymes A/S | Enzyme assisted crude palm oil extraction |
| EP3775222B1 (en) | 2018-03-26 | 2024-01-03 | Novozymes A/S | Fungal chaperone proteins |
| CN108823178B (zh) * | 2018-06-08 | 2021-12-10 | 中国中医科学院中药研究所 | 大黄素糖基转移酶蛋白FtUGT73BE5及其编码基因与应用 |
| CN108753748B (zh) * | 2018-06-08 | 2021-12-10 | 中国中医科学院中药研究所 | 大黄素糖基转移酶蛋白FtUGT75R2及其编码基因与应用 |
| CN108795903B (zh) * | 2018-06-08 | 2020-12-15 | 南京林业大学 | 一种高酶活淀粉酶的表达方法 |
| CN113302293A (zh) | 2018-12-12 | 2021-08-24 | 诺维信公司 | 具有木聚糖酶活性的多肽以及编码这些多肽的多核苷酸 |
| DK3898985T5 (da) | 2018-12-21 | 2024-08-05 | Novozymes As | Proteinekspression i tandem |
| CN110218750B (zh) * | 2019-04-24 | 2023-04-11 | 郑州轻工业学院 | 杂色曲霉菌株在发酵生产曲酸中应用 |
| CN110106152B (zh) * | 2019-04-30 | 2021-08-17 | 中国农业科学院油料作物研究所 | 杂交瘤细胞株ytt-2及其产生的抗环匹阿尼酸单克隆抗体 |
| CN110108874B (zh) * | 2019-04-30 | 2022-09-20 | 中国农业科学院油料作物研究所 | 快速检测环匹阿尼酸的胶体金免疫分析试纸条、制备及其应用 |
| EP4004211A1 (en) | 2019-07-25 | 2022-06-01 | Novozymes A/S | Filamentous fungal expression system |
| CN111139189B (zh) * | 2020-01-14 | 2022-04-19 | 浙江工业大学 | 曲霉wbx-38及其在生产环匹阿尼酸中的应用 |
| CN116024096B (zh) * | 2022-07-14 | 2025-06-03 | 浙江树人学院 | 泡盛曲霉sr3-28及其在从朱砂七提取大黄素中的应用 |
| JP2025542263A (ja) | 2022-12-21 | 2025-12-25 | ノボザイムス アクティーゼルスカブ | 細胞剥離のための微生物プロテアーゼ |
| CN116064437B (zh) * | 2023-02-20 | 2025-09-23 | 武汉轻工大学 | 用于降解黄曲霉毒素的复合酶制剂、复合酶制剂溶液及其制备方法和应用 |
| WO2025133003A2 (en) | 2023-12-19 | 2025-06-26 | 21St.Bio A/S | Non-animal bovine beta lactoglobulin |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995015391A2 (en) * | 1993-12-01 | 1995-06-08 | Novo Nordisk Biotech, Inc. | Aspergillus expression system |
| WO1995015390A1 (en) * | 1993-12-01 | 1995-06-08 | Novo Nordisk Biotech, Inc. | Aspergillus foetidus expression system |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU4424597A (en) * | 1996-09-13 | 1998-04-02 | Novo Nordisk Biotech, Inc. | Cells having dna insertion mutations which produce altered amounts of a polypeptide |
| DK0956338T3 (da) * | 1996-09-19 | 2006-05-08 | Novozymes As | Værtsceller og fremgangsmåder til produktion af proteiner |
-
1999
- 1999-12-22 JP JP2000591212A patent/JP2002533133A/ja not_active Withdrawn
- 1999-12-22 DK DK99960956T patent/DK1141371T3/da active
- 1999-12-22 AT AT99960956T patent/ATE414782T1/de active
- 1999-12-22 WO PCT/DK1999/000726 patent/WO2000039322A1/en not_active Ceased
- 1999-12-22 ES ES99960956T patent/ES2317706T3/es not_active Expired - Lifetime
- 1999-12-22 EP EP99960956A patent/EP1141371B1/en not_active Expired - Lifetime
- 1999-12-22 CN CNB99815766XA patent/CN100529096C/zh not_active Expired - Lifetime
- 1999-12-22 AU AU17740/00A patent/AU1774000A/en not_active Abandoned
- 1999-12-22 DE DE69939947T patent/DE69939947D1/de not_active Expired - Lifetime
-
2011
- 2011-05-06 JP JP2011103808A patent/JP5453343B2/ja not_active Expired - Lifetime
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995015391A2 (en) * | 1993-12-01 | 1995-06-08 | Novo Nordisk Biotech, Inc. | Aspergillus expression system |
| WO1995015390A1 (en) * | 1993-12-01 | 1995-06-08 | Novo Nordisk Biotech, Inc. | Aspergillus foetidus expression system |
Non-Patent Citations (1)
| Title |
|---|
| 杂色曲霉毒素研究进展. 赵宝玉,曹光荣.动物医学进展,第18卷第2期. 1997 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JP5453343B2 (ja) | 2014-03-26 |
| ES2317706T3 (es) | 2009-04-16 |
| DE69939947D1 (de) | 2009-01-02 |
| JP2002533133A (ja) | 2002-10-08 |
| EP1141371B1 (en) | 2008-11-19 |
| EP1141371A1 (en) | 2001-10-10 |
| AU1774000A (en) | 2000-07-31 |
| WO2000039322A1 (en) | 2000-07-06 |
| ATE414782T1 (de) | 2008-12-15 |
| JP2011188863A (ja) | 2011-09-29 |
| CN1333837A (zh) | 2002-01-30 |
| DK1141371T3 (da) | 2009-02-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100529096C (zh) | 在曲霉属突变细胞中产生多肽的方法 | |
| JP4436934B2 (ja) | 変更された量のポリペプチドを生成するdna挿入突然変異を有する細胞 | |
| US9873881B2 (en) | Promoter variants for expressing genes in a fungal cell | |
| CN1425068B (zh) | 在制备多肽的方法中有效的真菌转录激活因子 | |
| CN101659926A (zh) | 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子 | |
| US20080213901A1 (en) | Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells | |
| US20110236928A1 (en) | Methods for producing polypeptides in enzyme-deficient mutants of fusarium venenatum | |
| JP2012504390A (ja) | 糸状菌細胞におけるポジティブ及びネガティブ選択遺伝子の使用方法 | |
| JP4563585B2 (ja) | ポリペプチドの生成方法において有用な菌類転写活性化因子 | |
| JP2019503168A (ja) | Clr2活性が低減した糸状菌においてタンパク質を生産する方法 | |
| JP2019501646A (ja) | Clr1活性が低減した糸状菌においてタンパク質を生産する方法 | |
| US6828137B2 (en) | Methods for producing polypeptides in aspergillus mutant cells | |
| JP2002515252A (ja) | 糸状菌変異体細胞においてポリペプチドを生産するための方法 | |
| EP2062967A1 (en) | Genetically engineered aspergillus | |
| CN1318595C (zh) | 在缺乏环己缩酚肽的细胞中制备多肽的方法 | |
| US6903193B1 (en) | Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells | |
| US5925515A (en) | Direct selection of transformants on acetate-containing media | |
| US20040077047A1 (en) | Method for producing heterologous proteins in a homothallic fungus of the sordariaceae family |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| C10 | Entry into substantive examination | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20090819 |