CN100529096C - 在曲霉属突变细胞中产生多肽的方法 - Google Patents
在曲霉属突变细胞中产生多肽的方法 Download PDFInfo
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- CN100529096C CN100529096C CNB99815766XA CN99815766A CN100529096C CN 100529096 C CN100529096 C CN 100529096C CN B99815766X A CNB99815766X A CN B99815766XA CN 99815766 A CN99815766 A CN 99815766A CN 100529096 C CN100529096 C CN 100529096C
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- aspergillus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungi isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
- C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/66—Aspergillus
- C12R2001/69—Aspergillus oryzae
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Chemical & Material Sciences (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA199801726 | 1998-12-23 | ||
| DKPA199801726 | 1998-12-23 | ||
| DKPA199900745 | 1999-05-27 | ||
| DKPA199900745 | 1999-05-27 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1333837A CN1333837A (zh) | 2002-01-30 |
| CN100529096C true CN100529096C (zh) | 2009-08-19 |
Family
ID=26064565
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB99815766XA Expired - Lifetime CN100529096C (zh) | 1998-12-23 | 1999-12-22 | 在曲霉属突变细胞中产生多肽的方法 |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP1141371B1 (https=) |
| JP (2) | JP2002533133A (https=) |
| CN (1) | CN100529096C (https=) |
| AT (1) | ATE414782T1 (https=) |
| AU (1) | AU1774000A (https=) |
| DE (1) | DE69939947D1 (https=) |
| DK (1) | DK1141371T3 (https=) |
| ES (1) | ES2317706T3 (https=) |
| WO (1) | WO2000039322A1 (https=) |
Families Citing this family (83)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2003206684A1 (en) * | 2002-02-20 | 2003-09-09 | Novozymes A/S | Plant polypeptide production |
| US7396670B2 (en) | 2002-10-11 | 2008-07-08 | Novozymes A/S | Asparaginases and method of preparing a heat-treated product |
| WO2004090549A1 (en) * | 2003-04-11 | 2004-10-21 | Novozymes A/S | Method of screening for secreted glycosylated polypeptides |
| DK1620551T3 (da) | 2003-04-28 | 2014-01-13 | Chr Hansen As | Phospholipase og fremgangsmåde til fremstilling deraf |
| ATE516347T1 (de) | 2003-10-23 | 2011-07-15 | Novozymes As | Protease mit verbesserter stabilität in detergentien |
| WO2006053565A2 (en) | 2004-11-19 | 2006-05-26 | Novozymes A/S | Polypeptides having antimicrobial activity and polynucleotides encoding same |
| EP1831384B1 (en) | 2004-12-22 | 2015-08-12 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| US7888489B2 (en) | 2005-01-24 | 2011-02-15 | Dsm Ip Assets B.V. | Method for producing a compound of interest in a filamentous fungal cell |
| EP1739428A1 (en) * | 2005-07-01 | 2007-01-03 | Institut Pasteur | In vitro diagnostic method and kit for an aspergillus infection |
| EP1941025B1 (en) | 2005-10-17 | 2011-06-29 | Novozymes A/S | Use of fungal mutants for expression of antibodies |
| WO2008000632A1 (en) | 2006-06-29 | 2008-01-03 | Dsm Ip Assets B.V. | A method for achieving improved polypeptide expression |
| EP2059590B1 (en) | 2006-07-14 | 2014-12-31 | Novozymes, Inc. | Methods for producing secreted polypeptides having biological activity |
| WO2009051152A1 (ja) * | 2007-10-19 | 2009-04-23 | Noda Institute For Scientific Research | ポリケタイドシンターゼ・ノンリボゾーマルペプチドシンテターゼ遺伝子 |
| EP2857515B1 (en) | 2008-11-20 | 2018-02-21 | Novozymes Inc. | Polypeptides having amylolytic enhancing activity and polynucleotides encoding same |
| AU2010206181B2 (en) | 2009-01-21 | 2015-01-15 | Novozymes A/S | Polypeptides having esterase activity and nucleic acids encoding the same |
| WO2010108918A1 (en) | 2009-03-24 | 2010-09-30 | Novozymes A/S | Polypeptides having acetyl xylan esterase activity and polynucleotides encoding same |
| EP2421986A1 (en) | 2009-04-22 | 2012-02-29 | DSM IP Assets B.V. | Process for the production of a recombinant polypeptide of interest |
| CA2758396C (en) | 2009-04-24 | 2018-06-12 | Dsm Ip Assets B.V. | Carbohydrate degrading polypeptide and uses thereof |
| EP3293264A1 (en) | 2009-07-22 | 2018-03-14 | DSM IP Assets B.V. | Improved host cell for the production of a compound of interest |
| US8586829B2 (en) | 2009-09-30 | 2013-11-19 | Novozymes A/S | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| WO2011057083A1 (en) | 2009-11-06 | 2011-05-12 | Novozymes, Inc. | Polypeptides having xylanase activity and polynucleotides encoding same |
| EP2496694B1 (en) | 2009-11-06 | 2017-04-19 | Novozymes, Inc. | Compositions for saccharification of cellulosic material |
| US9267125B2 (en) | 2009-11-06 | 2016-02-23 | Novozymes, Inc. | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
| US9133448B2 (en) | 2010-02-11 | 2015-09-15 | Dsm Ip Assets B.V. | Polypeptide having cellobiohydrolase activity and uses thereof |
| EA201201125A1 (ru) | 2010-02-11 | 2013-03-29 | ДСМ АйПи АССЕТС Б.В. | Клетка-хозяин, способная продуцировать ферменты, пригодные для деградации лигноцеллюлозного материала |
| WO2011161208A1 (en) | 2010-06-25 | 2011-12-29 | Novozymes A/S | Polynucleotides having promoter activity |
| CN103068988A (zh) | 2010-06-25 | 2013-04-24 | 诺维信公司 | 具有启动子活性的多核苷酸 |
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| CN110106152B (zh) * | 2019-04-30 | 2021-08-17 | 中国农业科学院油料作物研究所 | 杂交瘤细胞株ytt-2及其产生的抗环匹阿尼酸单克隆抗体 |
| CN114391038A (zh) | 2019-07-25 | 2022-04-22 | 诺维信公司 | 丝状真菌表达系统 |
| CN111139189B (zh) * | 2020-01-14 | 2022-04-19 | 浙江工业大学 | 曲霉wbx-38及其在生产环匹阿尼酸中的应用 |
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| CN120476196A (zh) | 2022-12-21 | 2025-08-12 | 诺维信公司 | 用于细胞分离的微生物蛋白酶 |
| CN116064437B (zh) * | 2023-02-20 | 2025-09-23 | 武汉轻工大学 | 用于降解黄曲霉毒素的复合酶制剂、复合酶制剂溶液及其制备方法和应用 |
| WO2025133003A2 (en) | 2023-12-19 | 2025-06-26 | 21St.Bio A/S | Non-animal bovine beta lactoglobulin |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995015391A2 (en) * | 1993-12-01 | 1995-06-08 | Novo Nordisk Biotech, Inc. | Aspergillus expression system |
| WO1995015390A1 (en) * | 1993-12-01 | 1995-06-08 | Novo Nordisk Biotech, Inc. | Aspergillus foetidus expression system |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4436934B2 (ja) * | 1996-09-13 | 2010-03-24 | ノボザイムス,インコーポレイティド | 変更された量のポリペプチドを生成するdna挿入突然変異を有する細胞 |
| EP0956338B1 (en) * | 1996-09-19 | 2005-12-21 | Novozymes A/S | Host cells and methods of producing proteins |
-
1999
- 1999-12-22 AU AU17740/00A patent/AU1774000A/en not_active Abandoned
- 1999-12-22 WO PCT/DK1999/000726 patent/WO2000039322A1/en not_active Ceased
- 1999-12-22 ES ES99960956T patent/ES2317706T3/es not_active Expired - Lifetime
- 1999-12-22 DE DE69939947T patent/DE69939947D1/de not_active Expired - Lifetime
- 1999-12-22 DK DK99960956T patent/DK1141371T3/da active
- 1999-12-22 CN CNB99815766XA patent/CN100529096C/zh not_active Expired - Lifetime
- 1999-12-22 EP EP99960956A patent/EP1141371B1/en not_active Expired - Lifetime
- 1999-12-22 AT AT99960956T patent/ATE414782T1/de active
- 1999-12-22 JP JP2000591212A patent/JP2002533133A/ja not_active Withdrawn
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2011
- 2011-05-06 JP JP2011103808A patent/JP5453343B2/ja not_active Expired - Lifetime
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995015391A2 (en) * | 1993-12-01 | 1995-06-08 | Novo Nordisk Biotech, Inc. | Aspergillus expression system |
| WO1995015390A1 (en) * | 1993-12-01 | 1995-06-08 | Novo Nordisk Biotech, Inc. | Aspergillus foetidus expression system |
Non-Patent Citations (1)
| Title |
|---|
| 杂色曲霉毒素研究进展. 赵宝玉,曹光荣.动物医学进展,第18卷第2期. 1997 * |
Also Published As
| Publication number | Publication date |
|---|---|
| ATE414782T1 (de) | 2008-12-15 |
| JP2011188863A (ja) | 2011-09-29 |
| EP1141371A1 (en) | 2001-10-10 |
| DE69939947D1 (de) | 2009-01-02 |
| JP5453343B2 (ja) | 2014-03-26 |
| DK1141371T3 (da) | 2009-02-02 |
| CN1333837A (zh) | 2002-01-30 |
| JP2002533133A (ja) | 2002-10-08 |
| AU1774000A (en) | 2000-07-31 |
| EP1141371B1 (en) | 2008-11-19 |
| ES2317706T3 (es) | 2009-04-16 |
| WO2000039322A1 (en) | 2000-07-06 |
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