CN100529096C - 在曲霉属突变细胞中产生多肽的方法 - Google Patents

在曲霉属突变细胞中产生多肽的方法 Download PDF

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CN100529096C
CN100529096C CNB99815766XA CN99815766A CN100529096C CN 100529096 C CN100529096 C CN 100529096C CN B99815766X A CNB99815766X A CN B99815766XA CN 99815766 A CN99815766 A CN 99815766A CN 100529096 C CN100529096 C CN 100529096C
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aspergillus
toxin
cell
mutant
polypeptide
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CN1333837A (zh
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比约恩·E·克里斯坦森
亨里克·莫尔加尔德
斯文德·卡斯加尔德
简·莱姆贝克
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Novo Nordisk AS
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    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477Hemicellulases not provided in a preceding group
    • C12N9/248Xylanases
    • C12N9/2482Endo-1,4-beta-xylanase (3.2.1.8)
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CNB99815766XA 1998-12-23 1999-12-22 在曲霉属突变细胞中产生多肽的方法 Expired - Lifetime CN100529096C (zh)

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DKPA199801726 1998-12-23
DKPA199801726 1998-12-23
DKPA199900745 1999-05-27
DKPA199900745 1999-05-27

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CN1333837A CN1333837A (zh) 2002-01-30
CN100529096C true CN100529096C (zh) 2009-08-19

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EP (1) EP1141371B1 (https=)
JP (2) JP2002533133A (https=)
CN (1) CN100529096C (https=)
AT (1) ATE414782T1 (https=)
AU (1) AU1774000A (https=)
DE (1) DE69939947D1 (https=)
DK (1) DK1141371T3 (https=)
ES (1) ES2317706T3 (https=)
WO (1) WO2000039322A1 (https=)

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003206684A1 (en) * 2002-02-20 2003-09-09 Novozymes A/S Plant polypeptide production
ES2533469T3 (es) 2002-10-11 2015-04-10 Novozymes A/S Método de preparación de un producto tratado con calor
WO2004090549A1 (en) * 2003-04-11 2004-10-21 Novozymes A/S Method of screening for secreted glycosylated polypeptides
EP1620551B1 (en) 2003-04-28 2013-09-18 Novozymes A/S Phospholipase and method of producing it
WO2005040372A1 (en) 2003-10-23 2005-05-06 Novozymes A/S Protease with improved stability in detergents
EP1814996A2 (en) 2004-11-19 2007-08-08 Novozymes A/S Polypeptides having antimicrobial activity and polynucleotides encoding same
EP2365068B1 (en) 2004-12-22 2017-03-01 Novozymes A/S Enzymes for starch processing
EP1841861A1 (en) 2005-01-24 2007-10-10 DSMIP Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
EP1739428A1 (en) * 2005-07-01 2007-01-03 Institut Pasteur In vitro diagnostic method and kit for an aspergillus infection
WO2007045248A1 (en) 2005-10-17 2007-04-26 Novozymes A/S Use of fungal mutants for expression of antibodies
DK2423315T3 (en) 2006-06-29 2015-04-13 Dsm Ip Assets Bv A method for obtaining enhanced polypeptide expression
CN103451164A (zh) 2006-07-14 2013-12-18 诺维信股份有限公司 用于产生具有生物学活性的分泌型多肽的方法
JP5410981B2 (ja) * 2007-10-19 2014-02-05 公益財団法人野田産業科学研究所 ポリケタイドシンターゼ・ノンリボゾーマルペプチドシンテターゼ遺伝子
CN102292444A (zh) 2008-11-20 2011-12-21 诺维信股份有限公司 具有淀粉分解增强活性的多肽和编码该多肽的多核苷酸
ES2558698T3 (es) 2009-01-21 2016-02-08 Novozymes A/S Polipéptidos con actividad de esterasa y ácidos nucleicos que codifican los mismos
CA2752007A1 (en) 2009-03-24 2010-09-30 Novozymes A/S Polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
CA2758404A1 (en) 2009-04-22 2010-10-28 Dsm Ip Assets B.V. Process for the production of a recombinant polypeptide of interest
US9163224B2 (en) 2009-04-24 2015-10-20 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
WO2011009700A1 (en) 2009-07-22 2011-01-27 Dsm Ip Assets B.V. Improved host cell for the production of a compound of interest
EP2483402A1 (en) 2009-09-30 2012-08-08 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN104694517B (zh) 2009-11-06 2019-06-28 诺维信股份有限公司 具有木聚糖酶活性的多肽和编码该多肽的多核苷酸
US9267125B2 (en) 2009-11-06 2016-02-23 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
CN102947442B (zh) 2009-11-06 2016-03-09 诺维信股份有限公司 用于糖化纤维素材料的组合物
EA201201126A1 (ru) 2010-02-11 2013-05-30 ДСМ АйПи АССЕТС Б.В. Полипептид, обладающий активностью целлобиогидролазы, и его применение
WO2011098577A1 (en) 2010-02-11 2011-08-18 Dsm Ip Assets B.V. Host cell capable of producing enzymes useful for degradation of lignocellulosic material
EP2585601A1 (en) 2010-06-25 2013-05-01 Novozymes A/S Polynucleotides having promoter activity
WO2011161206A1 (en) 2010-06-25 2011-12-29 Novozymes A/S Polynucleotides having leader sequence function
EP2585602A1 (en) 2010-06-25 2013-05-01 Novozymes A/S Polynucleotides having promoter activity
AU2011273689B2 (en) 2010-06-29 2014-08-21 Dsm Ip Assets B.V. Polypeptide having carbohydrate degrading activity and uses thereof
MX2012014990A (es) 2010-06-29 2013-02-26 Dsm Ip Assets Bv Polipeptido que tiene actividad suolenina y sus usos.
AU2011273690C1 (en) 2010-06-29 2015-07-30 Versalis S.P.A. Polypeptide having or assisting in carbohydrate material degrading activity and uses thereof
EP2588494B1 (en) 2010-06-29 2018-03-07 DSM IP Assets B.V. Polypeptide having beta-glucosidase activity and uses thereof
AU2011273686B2 (en) 2010-06-29 2014-07-10 Versalis S.P.A. Polypeptide having acetyl xylan esterase activity and uses thereof
PL2588492T3 (pl) 2010-06-29 2016-09-30 Polipeptyd mający aktywność beta-glukozydazy i jego zastosowania
EP2588604B1 (en) 2010-06-30 2016-06-29 Novozymes, Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
CN103459600B (zh) * 2010-07-01 2016-10-19 帝斯曼知识产权资产管理有限公司 用于生产感兴趣的化合物的方法
US9394555B2 (en) 2010-08-12 2016-07-19 Novozymes, Inc. Compositions comprising a polypeptide having cellulolytic enhancing activity and a dioxy compound and uses thereof
WO2012030844A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
US9187742B2 (en) 2010-08-30 2015-11-17 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
BR112013009026A2 (pt) 2010-11-12 2016-07-12 Novozymes Inc polipeptídeo isolado, célula hospedeira recombinante, métodos para produzir um polipeptídeo, um mutante de uma célula precursora, uma proteína, e um produto de fermentação, para inibir a expressão de um polipeptídeo, para degradar ou converter um material celulósico, e método para fermentar um material celulósico, planta transgênica, parte de planta ou célula de planta, molécula de rna inibidora de filamento duplo, polinucleotídeo isolado, e, formulação de caldo integral ou composição de cultura de célula
US9353363B2 (en) 2011-01-26 2016-05-31 Novozymes A/S Glycoside hydrolases from thermophilic fungi
WO2012103322A1 (en) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2012103350A1 (en) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
CN103517986B (zh) 2011-01-26 2016-12-07 诺维信公司 具有纤维二糖水解酶活性的多肽及编码该多肽的多核苷酸
DK2668266T3 (en) 2011-01-26 2018-03-26 Novozymes Inc POLYPEPTIDES WITH CELLOBIO HYDROASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM
US9150842B2 (en) 2011-03-09 2015-10-06 Novozymes A/S Methods of increasing the cellulolytic enhancing activity of a polypeptide
CN103649308A (zh) 2011-04-28 2014-03-19 诺维信股份有限公司 具有内切葡聚糖酶活性的多肽和编码该多肽的多核苷酸
US9624518B2 (en) 2011-04-29 2017-04-18 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
EP2527448A1 (en) 2011-05-23 2012-11-28 Novozymes A/S Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi
CN102321700A (zh) * 2011-08-03 2012-01-18 无锡赛德生物工程有限公司 一种曲酸发酵工艺及发酵设备
CN103890169A (zh) 2011-08-24 2014-06-25 诺维信股份有限公司 烟曲霉纤维素分解酶组合物及其用途
DK2773656T3 (da) 2011-10-31 2019-09-09 Novozymes Inc Polypeptider med cellulolyseforbedrende aktivitet og polynukleotider, som koder for dem
DK3219794T3 (da) 2011-11-21 2019-12-16 Novozymes Inc GH61-polypeptidvarianter og polynukleotider, som koder for dem
CA2868308A1 (en) 2012-04-27 2013-10-31 Novozymes, Inc. Gh61 polypeptide variants and polynucleotides encoding same
DK2893012T3 (en) 2012-09-05 2018-12-03 Novozymes As Polypeptides with protease activity
CN104685052A (zh) 2012-09-19 2015-06-03 诺维信股份有限公司 用于增强纤维素材料的降解或转化的方法
US10035996B2 (en) 2012-10-08 2018-07-31 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US20150275194A1 (en) 2012-10-24 2015-10-01 Novozymes A/S Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same
WO2014093835A1 (en) 2012-12-14 2014-06-19 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN110628749A (zh) 2013-03-08 2019-12-31 诺维信公司 纤维二糖水解酶变体和编码它们的多核苷酸
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EP3594335B1 (en) 2014-09-05 2024-05-01 Novozymes A/S Carbohydrate binding module variants and polynucleotides encoding same
MX383142B (es) 2014-12-19 2025-03-13 Novozymes As Composiciones que comprenden polipéptidos que tienen actividad xilanasa y polipéptidos que tienen actividad arabinofuranosidasa.
DK3739045T3 (da) 2015-02-24 2024-10-21 Novozymes As Cellobiohydrolasevarianter og polynukleotider, som koder for dem
CN116676293A (zh) 2015-05-27 2023-09-01 国投生物科技投资有限公司 具有纤维二糖水解酶活性的多肽以及对其进行编码的多核苷酸
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995015391A2 (en) * 1993-12-01 1995-06-08 Novo Nordisk Biotech, Inc. Aspergillus expression system
WO1995015390A1 (en) * 1993-12-01 1995-06-08 Novo Nordisk Biotech, Inc. Aspergillus foetidus expression system

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4424597A (en) * 1996-09-13 1998-04-02 Novo Nordisk Biotech, Inc. Cells having dna insertion mutations which produce altered amounts of a polypeptide
DK0956338T3 (da) * 1996-09-19 2006-05-08 Novozymes As Værtsceller og fremgangsmåder til produktion af proteiner

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995015391A2 (en) * 1993-12-01 1995-06-08 Novo Nordisk Biotech, Inc. Aspergillus expression system
WO1995015390A1 (en) * 1993-12-01 1995-06-08 Novo Nordisk Biotech, Inc. Aspergillus foetidus expression system

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
杂色曲霉毒素研究进展. 赵宝玉,曹光荣.动物医学进展,第18卷第2期. 1997 *

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ES2317706T3 (es) 2009-04-16
DE69939947D1 (de) 2009-01-02
JP2002533133A (ja) 2002-10-08
EP1141371B1 (en) 2008-11-19
EP1141371A1 (en) 2001-10-10
AU1774000A (en) 2000-07-31
WO2000039322A1 (en) 2000-07-06
ATE414782T1 (de) 2008-12-15
JP2011188863A (ja) 2011-09-29
CN1333837A (zh) 2002-01-30
DK1141371T3 (da) 2009-02-02

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