ES2294462T3 - Procedimiento mejorado para tratamiento de bisulfito. - Google Patents
Procedimiento mejorado para tratamiento de bisulfito. Download PDFInfo
- Publication number
- ES2294462T3 ES2294462T3 ES04705800T ES04705800T ES2294462T3 ES 2294462 T3 ES2294462 T3 ES 2294462T3 ES 04705800 T ES04705800 T ES 04705800T ES 04705800 T ES04705800 T ES 04705800T ES 2294462 T3 ES2294462 T3 ES 2294462T3
- Authority
- ES
- Spain
- Prior art keywords
- solution
- nucleic acid
- bisulfite
- improved procedure
- bisulphite treatment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 6
- 102000039446 nucleic acids Human genes 0.000 abstract 6
- 108020004707 nucleic acids Proteins 0.000 abstract 6
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 abstract 2
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 229940104302 cytosine Drugs 0.000 abstract 1
- 230000011987 methylation Effects 0.000 abstract 1
- 238000007069 methylation reaction Methods 0.000 abstract 1
- 0 O=*I1[C@](I=I)I=C*C1 Chemical compound O=*I1[C@](I=I)I=C*C1 0.000 description 3
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01D—COMPOUNDS OF ALKALI METALS, i.e. LITHIUM, SODIUM, POTASSIUM, RUBIDIUM, CAESIUM, OR FRANCIUM
- C01D5/00—Sulfates or sulfites of sodium, potassium or alkali metals in general
- C01D5/14—Preparation of sulfites
- C01D5/145—Pyrosulfites or metabisulfites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Materials Engineering (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Inorganic Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Compounds Of Iron (AREA)
- Silver Salt Photography Or Processing Solution Therefor (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
Procedimiento para la conversión de una base de citosina, en un ácido nucleico, a una base de uracilo, que comprende las etapas de a) incubar una solución que comprende el ácido nucleico, durante un período de tiempo de 1, 5 a 3, 5 horas, a una temperatura comprendida dentro de unos márgenes comprendidos entre 70 y 90°C, en donde, la concentración de bisulfito, en la solución, es de un valor comprendido dentro de unos márgenes situados entre 3 M y 6, 25 M, y en donde, el valor pH de la solución, es de un valor comprendido dentro de unos márgenes situados entre 5, 0 y 6, 0, en donde, el ácido nucleico, se desamina, y b) incubar la solución que comprende el ácido nucleico desaminado, bajo condiciones alcalinas, a cuyo efecto, el ácido nucleido desaminado, se desulfona.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP03001854A EP1443052A1 (en) | 2003-01-29 | 2003-01-29 | Improved method for bisulfite treatment of nucleic acid |
| EP03001854 | 2003-01-29 | ||
| EP03010020 | 2003-05-02 | ||
| EP03010020 | 2003-05-02 | ||
| PCT/EP2004/000729 WO2004067545A1 (en) | 2003-01-29 | 2004-01-28 | Improved method for bisulfite treatment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2294462T3 true ES2294462T3 (es) | 2008-04-01 |
| ES2294462T5 ES2294462T5 (es) | 2015-05-06 |
Family
ID=32826770
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES04705800.3T Expired - Lifetime ES2294462T5 (es) | 2003-01-29 | 2004-01-28 | Método mejorado para el tratamiento de bisulfito |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7413855B2 (es) |
| EP (1) | EP1590362B8 (es) |
| JP (2) | JP2006516391A (es) |
| AT (1) | ATE373673T1 (es) |
| DE (1) | DE602004009038T3 (es) |
| ES (1) | ES2294462T5 (es) |
| WO (1) | WO2004067545A1 (es) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1590362B8 (en) † | 2003-01-29 | 2015-06-03 | Epigenomics AG | Improved method for bisulfite treatment |
| US7371526B2 (en) | 2003-08-29 | 2008-05-13 | Applera Corporation | Method and materials for bisulfite conversion of cytosine to uracil |
| US7534873B2 (en) | 2003-08-29 | 2009-05-19 | Applied Biosystems, Llc | Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil |
| US7368239B2 (en) | 2003-08-29 | 2008-05-06 | Applera Corporation | Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil |
| US7262013B2 (en) | 2003-08-29 | 2007-08-28 | Applera Corporation | Bisulfite method |
| CA2537810C (en) | 2003-09-04 | 2012-12-18 | Human Genetic Signatures Pty Ltd | Nucleic acid detection assay |
| US20080070240A2 (en) * | 2004-04-08 | 2008-03-20 | Toyo Boseki Kabushiki Kaisha | Composition for deaminating dna and method of detecting methylated dna |
| EP1632578A1 (en) | 2004-09-03 | 2006-03-08 | Roche Diagnostics GmbH | DNA decontamination method |
| US7658288B2 (en) | 2004-11-08 | 2010-02-09 | Applied Biosystems, Llc | Bisulfite conversion reagent |
| WO2006058393A1 (en) * | 2004-12-03 | 2006-06-08 | Human Genetic Signatures Pty Ltd | Methods for simplifying microbial nucleic acids by chemical modification of cytosines |
| EP2803734B1 (en) | 2005-04-01 | 2017-09-20 | Epigenomics AG | Improved bisulfite conversion of DNA |
| AU2006251866B2 (en) * | 2005-05-26 | 2007-11-29 | Human Genetic Signatures Pty Ltd | Isothermal strand displacement amplification using primers containing a non-regular base |
| US20060286577A1 (en) * | 2005-06-17 | 2006-12-21 | Xiyu Jia | Methods for detection of methylated DNA |
| WO2007030882A1 (en) * | 2005-09-14 | 2007-03-22 | Human Genetic Signatures Pty Ltd | Assay for a health state |
| WO2007068437A1 (en) | 2005-12-14 | 2007-06-21 | Roche Diagnostics Gmbh | New method for bisulfite treatment |
| WO2007123553A1 (en) * | 2006-04-24 | 2007-11-01 | Xiyu Jia | Methods for detection of methylated dna |
| US20080050738A1 (en) * | 2006-05-31 | 2008-02-28 | Human Genetic Signatures Pty Ltd. | Detection of target nucleic acid |
| WO2008109945A1 (en) * | 2007-03-12 | 2008-09-18 | Human Genetic Signatures Pty Ltd | Analysis of ribonucleic acid |
| WO2008113111A1 (en) * | 2007-03-16 | 2008-09-25 | Human Genetic Signatures Pty Ltd | Assay for gene expression |
| JP5431351B2 (ja) * | 2007-11-27 | 2014-03-05 | ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド | バイサルファイト修飾された核酸を増幅およびコピーするための酵素 |
| CA2707165A1 (en) * | 2007-12-05 | 2009-06-11 | Human Genetic Signatures Pty Ltd | Bisulphite treatment of rna |
| AU2008341021A1 (en) * | 2007-12-20 | 2009-07-02 | Human Genetic Signatures Pty Ltd | Elimination of contaminants associated with nucleic acid amplification |
| US9637779B2 (en) * | 2008-12-04 | 2017-05-02 | The Johns Hopkins University | Antisense transcriptomes of cells |
| FR2940805B1 (fr) | 2009-01-05 | 2015-10-16 | Biomerieux Sa | Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede |
| DK2420578T3 (en) * | 2009-04-17 | 2017-10-02 | Nat Defense Medical Center | A method of screening for cancer |
| CA2848144C (en) | 2011-09-07 | 2018-03-06 | Human Genetic Signatures Pty Ltd | Molecular detection assay |
| CA3111723A1 (en) | 2012-01-30 | 2013-08-08 | Exact Sciences Development Company, Llc | Sulfonated small dna compositions and methods for sulfonating and desulfonating small dnas |
| JP6544860B2 (ja) * | 2012-12-20 | 2019-07-17 | ゼネラル・エレクトリック・カンパニイ | 固体基材上での核酸安定化のための配合物 |
| CA3094717A1 (en) | 2018-04-02 | 2019-10-10 | Grail, Inc. | Methylation markers and targeted methylation probe panels |
| CN113286881A (zh) | 2018-09-27 | 2021-08-20 | 格里尔公司 | 甲基化标记和标靶甲基化探针板 |
| CN115246823B (zh) * | 2022-09-01 | 2024-02-20 | 渤海大学 | 一种检测亚硫酸氢盐和指示鱼肉新鲜度的双功能近红外发射荧光探针及其合成方法和应用 |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4335148A (en) † | 1980-06-04 | 1982-06-15 | Pennwalt Corporation | Methods of preventing grain sprouting after harvest through the application of sulfur dioxide, nitrogen and ammonia gases |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| DK171161B1 (da) | 1985-03-28 | 1996-07-08 | Hoffmann La Roche | Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve |
| US4844880A (en) * | 1985-12-27 | 1989-07-04 | Hmc Patents Holding Co., Inc. | Process for the manufacture of sodium metabisulfite |
| IE61148B1 (en) | 1988-03-10 | 1994-10-05 | Ici Plc | Method of detecting nucleotide sequences |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| DE68926504T2 (de) | 1988-07-20 | 1996-09-12 | David Segev | Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen |
| US5639611A (en) | 1988-12-12 | 1997-06-17 | City Of Hope | Allele specific polymerase chain reaction |
| NL8900725A (nl) | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| US5137806A (en) | 1989-12-11 | 1992-08-11 | Board Of Regents, The University Of Texas System | Methods and compositions for the detection of sequences in selected DNA molecules |
| AU635105B2 (en) | 1990-01-26 | 1993-03-11 | Abbott Laboratories | Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| IE913930A1 (en) | 1990-11-13 | 1992-06-17 | Siska Diagnostics | Nucleic acid amplification by two-enzyme, self-sustained¹sequence replication |
| US5552277A (en) | 1994-07-19 | 1996-09-03 | The Johns Hopkins University School Of Medicine | Genetic diagnosis of prostate cancer |
| DE19520398B4 (de) | 1995-06-08 | 2009-04-16 | Roche Diagnostics Gmbh | Magnetisches Pigment |
| US5786146A (en) | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| AU726501B2 (en) | 1996-06-04 | 2000-11-09 | University Of Utah Research Foundation | Monitoring hybridization during PCR |
| DE19743518A1 (de) | 1997-10-01 | 1999-04-15 | Roche Diagnostics Gmbh | Automatisierbare universell anwendbare Probenvorbereitungsmethode |
| US6355792B1 (en) | 1998-02-04 | 2002-03-12 | Merck Patent Gesellschaft | Method for isolating and purifying nucleic acids |
| DK1144620T3 (da) | 1998-11-30 | 2004-01-05 | Roche Diagnostics Gmbh | Magnetiske partikler til rensning af nucleinsyrer |
| SE9804469L (sv) | 1998-12-21 | 2000-06-22 | Volvo Ab | Anordning vid ett motorfordon |
| US6331393B1 (en) | 1999-05-14 | 2001-12-18 | University Of Southern California | Process for high-throughput DNA methylation analysis |
| JP2003514383A (ja) | 1999-11-17 | 2003-04-15 | ロシュ ダイアグノスティクス ゲゼルシャフト ミット ベシュレンクテル ハフツング | 磁性ガラス粒子、それらの製造方法、及びそれらの使用 |
| DE10029915B4 (de) | 2000-06-19 | 2005-06-16 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen |
| DE10050942B4 (de) | 2000-10-10 | 2005-11-17 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen |
| EP1394172A1 (en) | 2002-08-29 | 2004-03-03 | Boehringer Mannheim Gmbh | Improved method for bisulfite treatment |
| EP1590362B8 (en) † | 2003-01-29 | 2015-06-03 | Epigenomics AG | Improved method for bisulfite treatment |
-
2004
- 2004-01-28 EP EP04705800.3A patent/EP1590362B8/en not_active Expired - Lifetime
- 2004-01-28 DE DE602004009038.7T patent/DE602004009038T3/de not_active Expired - Lifetime
- 2004-01-28 JP JP2005518651A patent/JP2006516391A/ja active Pending
- 2004-01-28 ES ES04705800.3T patent/ES2294462T5/es not_active Expired - Lifetime
- 2004-01-28 AT AT04705800T patent/ATE373673T1/de active
- 2004-01-28 WO PCT/EP2004/000729 patent/WO2004067545A1/en active IP Right Grant
- 2004-01-28 US US10/540,406 patent/US7413855B2/en not_active Expired - Fee Related
-
2009
- 2009-06-25 JP JP2009150412A patent/JP2009261405A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP1590362B1 (en) | 2007-09-19 |
| DE602004009038T2 (de) | 2008-06-19 |
| EP1590362B8 (en) | 2015-06-03 |
| WO2004067545A1 (en) | 2004-08-12 |
| EP1590362B2 (en) | 2015-01-14 |
| DE602004009038D1 (de) | 2007-10-31 |
| JP2006516391A (ja) | 2006-07-06 |
| ATE373673T1 (de) | 2007-10-15 |
| US20060058518A1 (en) | 2006-03-16 |
| EP1590362A1 (en) | 2005-11-02 |
| US7413855B2 (en) | 2008-08-19 |
| JP2009261405A (ja) | 2009-11-12 |
| ES2294462T5 (es) | 2015-05-06 |
| DE602004009038T3 (de) | 2015-06-25 |
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