ES2270828T3 - Secuencias de adn y produccion por recombinacion de un alergeno de la familia de las gramineas. - Google Patents
Secuencias de adn y produccion por recombinacion de un alergeno de la familia de las gramineas. Download PDFInfo
- Publication number
- ES2270828T3 ES2270828T3 ES00926876T ES00926876T ES2270828T3 ES 2270828 T3 ES2270828 T3 ES 2270828T3 ES 00926876 T ES00926876 T ES 00926876T ES 00926876 T ES00926876 T ES 00926876T ES 2270828 T3 ES2270828 T3 ES 2270828T3
- Authority
- ES
- Spain
- Prior art keywords
- allergen
- phi
- baselineskip
- phleum pratense
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000013566 allergen Substances 0.000 title claims abstract description 57
- 238000004519 manufacturing process Methods 0.000 title description 2
- 238000005215 recombination Methods 0.000 title description 2
- 230000006798 recombination Effects 0.000 title description 2
- 241000746983 Phleum pratense Species 0.000 claims abstract description 26
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 17
- 229920001184 polypeptide Polymers 0.000 claims abstract description 16
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 16
- 239000002773 nucleotide Substances 0.000 claims abstract description 13
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 13
- 230000000694 effects Effects 0.000 claims abstract description 12
- 108020004511 Recombinant DNA Proteins 0.000 claims abstract description 11
- 102000053602 DNA Human genes 0.000 claims abstract description 10
- 206010020751 Hypersensitivity Diseases 0.000 claims description 29
- 230000007815 allergy Effects 0.000 claims description 28
- 108020004414 DNA Proteins 0.000 claims description 11
- 150000007523 nucleic acids Chemical class 0.000 claims description 11
- 108020004707 nucleic acids Proteins 0.000 claims description 10
- 102000039446 nucleic acids Human genes 0.000 claims description 10
- 230000000172 allergic effect Effects 0.000 claims description 9
- 208000010668 atopic eczema Diseases 0.000 claims description 9
- 239000013604 expression vector Substances 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 9
- 241001465754 Metazoa Species 0.000 claims description 8
- 238000003745 diagnosis Methods 0.000 claims description 8
- 239000003814 drug Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- 230000001225 therapeutic effect Effects 0.000 claims description 5
- 229940079593 drug Drugs 0.000 claims description 4
- 238000000338 in vitro Methods 0.000 claims description 4
- 239000013598 vector Substances 0.000 claims description 4
- 238000010276 construction Methods 0.000 claims description 3
- 238000002255 vaccination Methods 0.000 claims description 3
- 238000010367 cloning Methods 0.000 claims description 2
- 230000000295 complement effect Effects 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 210000003527 eukaryotic cell Anatomy 0.000 claims description 2
- 230000003308 immunostimulating effect Effects 0.000 claims description 2
- 239000000825 pharmaceutical preparation Substances 0.000 claims description 2
- 210000001236 prokaryotic cell Anatomy 0.000 claims description 2
- 238000011238 DNA vaccination Methods 0.000 claims 1
- 208000026935 allergic disease Diseases 0.000 description 20
- 229960004784 allergens Drugs 0.000 description 17
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 8
- 229930010555 Inosine Natural products 0.000 description 8
- 229960003786 inosine Drugs 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 6
- 239000002299 complementary DNA Substances 0.000 description 5
- 239000003999 initiator Substances 0.000 description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 241000209504 Poaceae Species 0.000 description 4
- 229940046528 grass pollen Drugs 0.000 description 4
- 238000009169 immunotherapy Methods 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 238000002560 therapeutic procedure Methods 0.000 description 4
- 241000282412 Homo Species 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 230000009257 reactivity Effects 0.000 description 3
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 2
- 229940074608 allergen extract Drugs 0.000 description 2
- 230000002009 allergenic effect Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000001617 migratory effect Effects 0.000 description 2
- 239000013615 primer Substances 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 241000238876 Acari Species 0.000 description 1
- IPWKGIFRRBGCJO-IMJSIDKUSA-N Ala-Ser Chemical compound C[C@H]([NH3+])C(=O)N[C@@H](CO)C([O-])=O IPWKGIFRRBGCJO-IMJSIDKUSA-N 0.000 description 1
- 206010002199 Anaphylactic shock Diseases 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 206010010744 Conjunctivitis allergic Diseases 0.000 description 1
- 244000052363 Cynodon dactylon Species 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 108020001019 DNA Primers Proteins 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 240000004585 Dactylis glomerata Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- LGYZYFFDELZWRS-DCAQKATOSA-N Glu-Glu-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](N)CCC(O)=O LGYZYFFDELZWRS-DCAQKATOSA-N 0.000 description 1
- VEPBEGNDJYANCF-QWRGUYRKSA-N Gly-Lys-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CCCCN VEPBEGNDJYANCF-QWRGUYRKSA-N 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 241000744855 Holcus Species 0.000 description 1
- 241000209082 Lolium Species 0.000 description 1
- 240000004296 Lolium perenne Species 0.000 description 1
- OVIVOCSURJYCTM-GUBZILKMSA-N Lys-Asp-Glu Chemical compound NCCCC[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](C(O)=O)CCC(O)=O OVIVOCSURJYCTM-GUBZILKMSA-N 0.000 description 1
- UQRZFMQQXXJTTF-AVGNSLFASA-N Lys-Lys-Glu Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(O)=O UQRZFMQQXXJTTF-AVGNSLFASA-N 0.000 description 1
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 1
- 239000002033 PVDF binder Substances 0.000 description 1
- 241000209049 Poa pratensis Species 0.000 description 1
- 206010039085 Rhinitis allergic Diseases 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- MUARUIBTKQJKFY-WHFBIAKZSA-N Ser-Gly-Asp Chemical compound [H]N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(O)=O MUARUIBTKQJKFY-WHFBIAKZSA-N 0.000 description 1
- 239000012505 Superdex™ Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000013567 aeroallergen Substances 0.000 description 1
- 208000002205 allergic conjunctivitis Diseases 0.000 description 1
- 201000010105 allergic rhinitis Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 208000003455 anaphylaxis Diseases 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 208000024998 atopic conjunctivitis Diseases 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000012636 effector Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 239000013574 grass pollen allergen Substances 0.000 description 1
- 229960001340 histamine Drugs 0.000 description 1
- 210000003630 histaminocyte Anatomy 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000030603 inherited susceptibility to asthma Diseases 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000002655 kraft paper Substances 0.000 description 1
- 108010009298 lysylglutamic acid Proteins 0.000 description 1
- 108010054155 lysyllysine Proteins 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 102000035118 modified proteins Human genes 0.000 description 1
- 108091005573 modified proteins Proteins 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 1
- 150000003180 prostaglandins Chemical class 0.000 description 1
- 238000000734 protein sequencing Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/53—DNA (RNA) vaccination
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Pulmonology (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19918682 | 1999-04-23 | ||
| DE19918682A DE19918682A1 (de) | 1999-04-23 | 1999-04-23 | DNA-Sequenz und rekombinante Herstellung eines Graminaen-Allergens |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2270828T3 true ES2270828T3 (es) | 2007-04-16 |
Family
ID=7905749
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES00926876T Expired - Lifetime ES2270828T3 (es) | 1999-04-23 | 2000-04-12 | Secuencias de adn y produccion por recombinacion de un alergeno de la familia de las gramineas. |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US7241450B1 (https=) |
| EP (1) | EP1171604B1 (https=) |
| JP (1) | JP4588888B2 (https=) |
| CN (1) | CN1203179C (https=) |
| AT (1) | ATE338126T1 (https=) |
| AU (1) | AU781275B2 (https=) |
| CA (1) | CA2370393C (https=) |
| CZ (1) | CZ20013796A3 (https=) |
| DE (2) | DE19918682A1 (https=) |
| ES (1) | ES2270828T3 (https=) |
| HU (1) | HUP0200883A2 (https=) |
| PL (1) | PL201818B1 (https=) |
| PT (1) | PT1171604E (https=) |
| RU (1) | RU2238321C2 (https=) |
| WO (1) | WO2000065060A2 (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19939982A1 (de) | 1999-08-24 | 2001-03-01 | Merck Patent Gmbh | Verfahren zur Isolierung und Aufreinigung von Gräserpollenallergenen |
| AUPR779201A0 (en) * | 2001-09-20 | 2001-10-11 | University Of Melbourne, The | Immunotherapeutic and immunoprophylactic reagents |
| SE0200946D0 (sv) * | 2002-03-27 | 2002-03-27 | Pharmacia Diagnostics Ab | Novel Allergen |
| WO2004022588A1 (de) * | 2002-08-19 | 2004-03-18 | Merck Patent Gmbh | Varianten des majorallergens phl p 1 aus lieschgras |
| ATE513848T1 (de) * | 2003-06-04 | 2011-07-15 | Merck Patent Gmbh | Phl p 5a-derivate mit reduzierter allergenität und erhaltener t-zellreaktivität |
| DE10359351A1 (de) * | 2003-12-16 | 2005-07-21 | Merck Patent Gmbh | DNA-Sequenz und rekombinante Herstellung von Gruppe-4 Majorallergenen aus Getreiden |
| DE102004035337A1 (de) * | 2004-07-21 | 2006-03-16 | Merck Patent Gmbh | Varianten der Gruppe 1-Allergene aus Poaceae mit reduzierter Allergenität und erhaltener T-Zellreaktivität |
| WO2011085782A1 (de) * | 2010-01-14 | 2011-07-21 | Merck Patent Gmbh | Varianten der gruppe 6-allergene der süssgräser mit reduzierter allergenität durch mutagenese von prolinresten |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5736362A (en) * | 1990-10-26 | 1998-04-07 | The University Of Melbourne | Ryegrass pollen allergen |
-
1999
- 1999-04-23 DE DE19918682A patent/DE19918682A1/de not_active Withdrawn
-
2000
- 2000-04-12 CA CA2370393A patent/CA2370393C/en not_active Expired - Fee Related
- 2000-04-12 JP JP2000614395A patent/JP4588888B2/ja not_active Expired - Fee Related
- 2000-04-12 AT AT00926876T patent/ATE338126T1/de active
- 2000-04-12 WO PCT/EP2000/003259 patent/WO2000065060A2/de not_active Ceased
- 2000-04-12 EP EP00926876A patent/EP1171604B1/de not_active Expired - Lifetime
- 2000-04-12 DE DE50013400T patent/DE50013400D1/de not_active Expired - Lifetime
- 2000-04-12 HU HU0200883A patent/HUP0200883A2/hu unknown
- 2000-04-12 PL PL356055A patent/PL201818B1/pl unknown
- 2000-04-12 RU RU2001130165A patent/RU2238321C2/ru not_active IP Right Cessation
- 2000-04-12 PT PT00926876T patent/PT1171604E/pt unknown
- 2000-04-12 CN CNB008066566A patent/CN1203179C/zh not_active Expired - Fee Related
- 2000-04-12 US US09/959,340 patent/US7241450B1/en not_active Expired - Fee Related
- 2000-04-12 AU AU45477/00A patent/AU781275B2/en not_active Ceased
- 2000-04-12 CZ CZ20013796A patent/CZ20013796A3/cs unknown
- 2000-04-12 ES ES00926876T patent/ES2270828T3/es not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CZ20013796A3 (cs) | 2002-01-16 |
| CN1351664A (zh) | 2002-05-29 |
| JP2002542783A (ja) | 2002-12-17 |
| US7241450B1 (en) | 2007-07-10 |
| CA2370393C (en) | 2010-09-14 |
| JP4588888B2 (ja) | 2010-12-01 |
| RU2238321C2 (ru) | 2004-10-20 |
| DE19918682A1 (de) | 2000-10-26 |
| EP1171604A2 (de) | 2002-01-16 |
| EP1171604B1 (de) | 2006-08-30 |
| WO2000065060A2 (de) | 2000-11-02 |
| ATE338126T1 (de) | 2006-09-15 |
| HUP0200883A2 (hu) | 2003-07-28 |
| AU4547700A (en) | 2000-11-10 |
| WO2000065060A3 (de) | 2001-03-01 |
| PL356055A1 (en) | 2004-06-14 |
| DE50013400D1 (de) | 2006-10-12 |
| CN1203179C (zh) | 2005-05-25 |
| PL201818B1 (pl) | 2009-05-29 |
| AU781275B2 (en) | 2005-05-12 |
| PT1171604E (pt) | 2007-01-31 |
| CA2370393A1 (en) | 2000-11-02 |
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