ES2209145T5 - Complejos de ribosomas como particulas de seleccion para el desarrollo in vitro y evolucion de proteinas. - Google Patents

Complejos de ribosomas como particulas de seleccion para el desarrollo in vitro y evolucion de proteinas. Download PDF

Info

Publication number
ES2209145T5
ES2209145T5 ES98924468T ES98924468T ES2209145T5 ES 2209145 T5 ES2209145 T5 ES 2209145T5 ES 98924468 T ES98924468 T ES 98924468T ES 98924468 T ES98924468 T ES 98924468T ES 2209145 T5 ES2209145 T5 ES 2209145T5
Authority
ES
Spain
Prior art keywords
mrna
protein
presentation
library
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES98924468T
Other languages
English (en)
Spanish (es)
Other versions
ES2209145T3 (es
Inventor
Michael John Taussig
Mingyue He
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Crescendo Biologics Ltd
Original Assignee
Crescendo Biologics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27268863&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2209145(T5) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from GBGB9710829.4A external-priority patent/GB9710829D0/en
Priority claimed from GBGB9724850.4A external-priority patent/GB9724850D0/en
Priority claimed from GBGB9804195.7A external-priority patent/GB9804195D0/en
Application filed by Crescendo Biologics Ltd filed Critical Crescendo Biologics Ltd
Publication of ES2209145T3 publication Critical patent/ES2209145T3/es
Application granted granted Critical
Publication of ES2209145T5 publication Critical patent/ES2209145T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1041—Ribosome/Polysome display, e.g. SPERT, ARM
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
ES98924468T 1997-05-28 1998-05-28 Complejos de ribosomas como particulas de seleccion para el desarrollo in vitro y evolucion de proteinas. Expired - Lifetime ES2209145T5 (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GBGB9710829.4A GB9710829D0 (en) 1997-05-28 1997-05-28 Selection particles for in vitro display and evolution of antibody combining sites
GB9710829 1997-05-28
GBGB9724850.4A GB9724850D0 (en) 1997-11-26 1997-11-26 Selection particles for in vitro display and evolution of antibody combining sites
GB9724850 1997-11-26
GB9804195 1998-02-28
GBGB9804195.7A GB9804195D0 (en) 1998-02-28 1998-02-28 Selection particles for in vitro display and evolution of antibody combining sites

Publications (2)

Publication Number Publication Date
ES2209145T3 ES2209145T3 (es) 2004-06-16
ES2209145T5 true ES2209145T5 (es) 2009-10-30

Family

ID=27268863

Family Applications (1)

Application Number Title Priority Date Filing Date
ES98924468T Expired - Lifetime ES2209145T5 (es) 1997-05-28 1998-05-28 Complejos de ribosomas como particulas de seleccion para el desarrollo in vitro y evolucion de proteinas.

Country Status (10)

Country Link
US (1) US6620587B1 (OSRAM)
EP (1) EP0985032B2 (OSRAM)
JP (1) JP4060892B2 (OSRAM)
AT (1) ATE250668T1 (OSRAM)
CA (1) CA2291721C (OSRAM)
DE (1) DE69818458T3 (OSRAM)
DK (1) DK0985032T4 (OSRAM)
ES (1) ES2209145T5 (OSRAM)
PT (1) PT985032E (OSRAM)
WO (1) WO1998054312A1 (OSRAM)

Families Citing this family (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4854999A (en) * 1998-07-02 2000-01-24 Invitro Diagnostics, Inc. Methods, compositions and apparatus for making nucleic acid molecules having a selected affinity to a target molecule
DE19932688B4 (de) * 1999-07-13 2009-10-08 Scil Proteins Gmbh Design von Beta-Faltblatt-Proteinen des gamma-II-kristallins antikörperähnlichen
GB9917027D0 (en) 1999-07-20 1999-09-22 Affibody Technology Sweeden Ab In vitro selection and optional identification of polypeptides using solid support carriers
US20010039014A1 (en) 2000-01-11 2001-11-08 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
DK1268766T3 (da) 2000-03-31 2006-04-10 Cambridge Antibody Tech Forbedringer af ribosom-display
EP1652919A1 (en) * 2000-03-31 2006-05-03 Cambridge Antibody Technology LTD Improvements to ribosome display
US7129326B2 (en) 2000-04-14 2006-10-31 Genencor International, Inc. Methods for selective targeting
ES2338097T3 (es) 2000-04-14 2010-05-04 Genencor International, Inc. Procedimiento de identificacion selectiva.
CA2440649A1 (en) 2001-03-07 2002-09-19 Children's Medical Center Corporation Method to screen peptide display libraries using minicell display
EP1401850A1 (en) * 2001-06-20 2004-03-31 Nuevolution A/S Nucleoside derivatives for library preparation
US20060234231A1 (en) * 2001-06-20 2006-10-19 Nuevolution A/S Microarrays displaying encoded molecules
DE10145226A1 (de) * 2001-09-13 2003-04-10 Lifebits Ag Herstellung von trägergebundenen Molekülen
DE10147074A1 (de) * 2001-09-25 2003-05-08 Beru Ag Verfahren zum Betreiben einer aus mehreren Heizelementen bestehenden mehrstufigen elektrischen Heizung
DE20200926U1 (de) * 2002-01-23 2002-04-18 Hegenscheidt-MFD GmbH & Co. KG, 41812 Erkelenz Festwalzgerät einer Festwalzmaschine für Kurbelwellen
US20050221318A1 (en) * 2002-03-15 2005-10-06 Gouliaev Alex H Building block forming a c-c bond upon reaction
US20050247001A1 (en) * 2002-03-15 2005-11-10 Nuevolution A/S Building block forming a c-c or a c-hetero atom bond uponreaction
US10731151B2 (en) 2002-03-15 2020-08-04 Nuevolution A/S Method for synthesising templated molecules
AU2003218630A1 (en) * 2002-03-15 2003-09-29 Nuevolution A/S A building block capable of functional entity transfer to nucleophil
DE60324778D1 (de) * 2002-03-15 2009-01-02 Nuevolution As Eine verbesserte methode zur synthese von matritzenabhängigen molekülen
WO2004013070A2 (en) * 2002-08-01 2004-02-12 Nuevolution A/S Multi-step synthesis of templated molecules
US7842476B2 (en) * 2002-09-06 2010-11-30 Isogenica Limited In vitro peptide expression library
DK3299463T3 (da) 2002-10-30 2020-12-07 Nuevolution As Enzymatisk kodning
EP2581383A1 (en) 2002-11-25 2013-04-17 Genencor International, Inc. Skin or hair binding peptides
ATE450609T1 (de) * 2002-12-19 2009-12-15 Nuevolution As Durch quasizufallsstrukturen und funktionen geführte synthesemethode
EP1597394A2 (en) * 2003-02-21 2005-11-23 Nuevolution A/S A method for obtaining structural information about an encoded molecule
US20070026397A1 (en) * 2003-02-21 2007-02-01 Nuevolution A/S Method for producing second-generation library
WO2004083461A1 (en) * 2003-03-19 2004-09-30 Discerna Limited Recovery of dna
US7915201B2 (en) * 2003-03-20 2011-03-29 Nuevolution A/S Ligational encoding of small molecules
DE10324447A1 (de) 2003-05-28 2004-12-30 Scil Proteins Gmbh Generierung künstlicher Bindungsproteine auf der Grundlage von Ubiquitin
GB0316314D0 (en) * 2003-07-12 2003-08-13 Univ Belfast Method
WO2005026387A1 (en) 2003-09-18 2005-03-24 Nuevolution A/S A method for obtaining structural information concerning an encoded molecule and method for selecting compounds
EP1723255B1 (en) * 2004-02-17 2010-12-29 Nuevolution A/S Method for enrichment involving elimination by mismatch hybridisation
WO2005090566A2 (en) * 2004-03-22 2005-09-29 Nuevolution A/S Ligational encoding using building block oligonucleotides
US20050250112A1 (en) * 2004-05-07 2005-11-10 Padmabandu Gothami A Nucleic acids, compositions, methods, and kits for detecting mycoplasma and acholeplasma species
US9006393B1 (en) 2004-08-26 2015-04-14 Patricia L. Clark Molecular constructs and uses thereof in ribosomal translational events
DE102004049479A1 (de) 2004-10-11 2006-04-13 Scil Proteins Gmbh Proteinkonjugate zur Verwendung in Therapie, Diagnose und Chromatographie
LT2336315T (lt) 2005-12-01 2017-11-10 Nuevolution A/S Fermentiniai kodavimo būdai, skirti efektyviai didelių bibliotekų sintezei
GB0605584D0 (en) 2006-03-20 2006-04-26 Olink Ab Method for analyte detection using proximity probes
US7858342B2 (en) * 2006-06-29 2010-12-28 The Invention Science Fund I, Llc Apparatus for arbitrary peptide synthesis
US7879975B2 (en) * 2006-06-29 2011-02-01 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7923533B2 (en) * 2006-06-29 2011-04-12 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7777002B2 (en) * 2006-06-29 2010-08-17 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7910695B2 (en) 2006-06-29 2011-03-22 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
EP1925664A1 (en) 2006-11-15 2008-05-28 Scil proteins GmbH Artificial binding proteins based on a modified alpha helical region of ubiquitin
US8865179B2 (en) * 2008-01-26 2014-10-21 Swey-Shen Alexchen Aptameric IgE peptides in a protein scaffold as an allergy vaccine
WO2010011944A2 (en) * 2008-07-25 2010-01-28 Wagner Richard W Protein screeing methods
BRPI0919426A2 (pt) * 2008-09-30 2015-12-15 Abbott Lab métodos melhorado de exposição de rna
KR101436222B1 (ko) 2009-12-14 2014-09-01 쉴 프로테인즈 게엠베하 리간드 결합능력을 가진 헤테로-다량체 변형 유비퀴틴 단백질을 확인하는 방법
JP5863045B2 (ja) 2010-03-11 2016-02-16 国立研究開発法人理化学研究所 ポリペプチド配列の選択方法、並びに金属酸化物または含珪素化合物結合ペプチドおよびその利用
GB201004292D0 (en) 2010-03-15 2010-04-28 Olink Ab Assay for localised detection of analytes
CA2832672A1 (en) 2010-04-16 2011-10-20 Nuevolution A/S Bi-functional complexes and methods for making and using such complexes
GB201011971D0 (en) 2010-07-15 2010-09-01 Olink Ab Methods and product
WO2012049316A1 (en) 2010-10-15 2012-04-19 Olink Ab Dynamic range methods
EP2466310A1 (en) 2010-12-17 2012-06-20 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt GmbH Means and methods for detecting double-strand breaks
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
GB201101621D0 (en) 2011-01-31 2011-03-16 Olink Ab Method and product
JP5948673B2 (ja) 2011-03-10 2016-07-06 ジーンフロンティア株式会社 リポ多糖混入量を低減した蛋白質合成用組成物、該組成物を用いた蛋白質製造方法
GB201107863D0 (en) 2011-05-11 2011-06-22 Olink Ab Method and product
US9863936B2 (en) 2011-05-23 2018-01-09 Riken Nucleic acid construct, nucleic acid-protein complex, and use thereof
EP2721152B1 (en) 2011-06-15 2019-03-27 Navigo Proteins GmbH Dimeric binding proteins based on modified ubiquitins
GB201201547D0 (en) 2012-01-30 2012-03-14 Olink Ab Method and product
EP3102245B1 (en) 2014-02-05 2021-09-08 Molecular Templates, Inc. Methods of screening, selecting, and identifying cytotoxic recombinant polypeptides based on an interim diminution of ribotoxicity
EP3209776A1 (en) * 2014-10-22 2017-08-30 Crescendo Biologics Limited Human vh domain scaffolds
EP3253785B1 (en) 2015-02-06 2019-04-10 Navigo Proteins Gmbh Novel egfr binding proteins
CN107922483B (zh) 2015-07-16 2021-07-30 纳维格蛋白质有限公司 新型免疫球蛋白结合蛋白及其在亲和纯化中的用途
WO2017013136A1 (en) 2015-07-20 2017-01-26 Scil Proteins Gmbh Novel binding proteins based on di-ubiquitin muteins and methods for generation
CN108291257B (zh) 2015-09-24 2023-12-29 阿布维特罗有限责任公司 亲和-寡核苷酸缀合物及其用途
AU2017260274A1 (en) 2016-05-04 2018-11-01 Navigo Proteins Gmbh Targeted compounds for the site-specific coupling of chemical moieties comprising a peptide linker
CA3030208A1 (en) 2016-08-11 2018-02-15 Navigo Proteins Gmbh Novel alkaline stable immunoglobulin binding proteins
BR112019005748A2 (pt) 2016-09-24 2019-06-18 Abvitro Llc afinidade-conjugados de oligonucleotídeo e usos destes
US11414466B2 (en) 2017-11-07 2022-08-16 Navigo Proteins Gmbh Fusion proteins with specificity for ED-B and long serum half-life for diagnosis or treatment of cancer
CA3123986A1 (en) 2018-12-18 2020-06-25 Navigo Proteins Gmbh Novel folr1 specific binding proteins for cancer diagnosis and treatment
US11926817B2 (en) 2019-08-09 2024-03-12 Nutcracker Therapeutics, Inc. Microfluidic apparatus and methods of use thereof
EP4382534A1 (en) 2022-12-06 2024-06-12 Biomérieux Synthetic peptide for use in an immunomolecular assay
CN120858284A (zh) 2023-01-27 2025-10-28 生物梅里埃公司 用于更快生物标志物检测的改良的免疫分子测定
WO2025111396A1 (en) 2023-11-20 2025-05-30 Alamar Biosciences, Inc. Molecular barcode set and use thereof in multiplex proximity detection assays

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06178688A (ja) * 1992-12-11 1994-06-28 Mitsui Toatsu Chem Inc Ｅ型緑膿菌を抗原とするヒト抗体をコードする遺伝子
WO1995011922A1 (en) 1993-10-29 1995-05-04 Affymax Technologies N.V. In vitro peptide and antibody display libraries
DK1268766T3 (da) 2000-03-31 2006-04-10 Cambridge Antibody Tech Forbedringer af ribosom-display

Also Published As

Publication number Publication date
US6620587B1 (en) 2003-09-16
ATE250668T1 (de) 2003-10-15
WO1998054312A1 (en) 1998-12-03
EP0985032A1 (en) 2000-03-15
JP4060892B2 (ja) 2008-03-12
AU725957B2 (en) 2000-10-26
EP0985032B1 (en) 2003-09-24
CA2291721C (en) 2011-08-09
DE69818458D1 (de) 2003-10-30
ES2209145T3 (es) 2004-06-16
DE69818458T2 (de) 2004-07-08
DE69818458T3 (de) 2009-12-17
PT985032E (pt) 2004-02-27
JP2002500514A (ja) 2002-01-08
EP0985032B2 (en) 2009-06-03
AU7666698A (en) 1998-12-30
CA2291721A1 (en) 1998-12-03
DK0985032T3 (da) 2004-02-02
DK0985032T4 (da) 2009-08-31

Similar Documents

Publication Publication Date Title
ES2209145T3 (es) Complejos de ribosomas como particulas de seleccion para el desarrollo in vitro y evolucion de proteinas.
US6589741B2 (en) Polysome display in the absence of functional ssrA-RNA
Schaffitzel et al. Ribosome display: an in vitro method for selection and evolution of antibodies from libraries
Irving et al. Ribosome display and affinity maturation: from antibodies to single V-domains and steps towards cancer therapeutics
ES2434850T3 (es) Método mejorado de presentación de ARN
ES2373110T3 (es) Selección de proteínas usando fusiones de arn-proteína.
CA2361725C (en) Selection of proteins using rna-protein fusions
US5922545A (en) In vitro peptide and antibody display libraries
US8207093B2 (en) Selection of proteins using RNA-protein fusions
He et al. Ribosome display of antibodies: expression, specificity and recovery in a eukaryotic system
JP2003529369A (ja) リボソーム・ディスプレイの改良
WO1995011922A1 (en) In vitro peptide and antibody display libraries
AU725957C (en) Ribosome complexes as selection particles for in vitro display and evolution of proteins
US20180371451A1 (en) Method and Kit for Generating High Affinity Binding Agents
ES2326649T3 (es) Recuperacion de dna.
Yang et al. Selection of antigenic markers on a GFP-Cκ fusion scaffold with high sensitivity by eukaryotic ribosome display
TAUSSIG 3.2 Selection of DB3R VH/K from libraries
HK1159250B (en) Improved method of rna display