ES2176445T3 - Deteccion de esteroides por medio de un inmunoensayo competitivo. - Google Patents

Deteccion de esteroides por medio de un inmunoensayo competitivo.

Info

Publication number
ES2176445T3
ES2176445T3 ES96908511T ES96908511T ES2176445T3 ES 2176445 T3 ES2176445 T3 ES 2176445T3 ES 96908511 T ES96908511 T ES 96908511T ES 96908511 T ES96908511 T ES 96908511T ES 2176445 T3 ES2176445 T3 ES 2176445T3
Authority
ES
Spain
Prior art keywords
steroid
solid phase
kit
conjugate
mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96908511T
Other languages
English (en)
Inventor
Gregg T Williams
William R Groskopf
Harold N Baker
Dalmacio A Agdeppa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Application granted granted Critical
Publication of ES2176445T3 publication Critical patent/ES2176445T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/743—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J41/00—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring
    • C07J41/0005—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring the nitrogen atom being directly linked to the cyclopenta(a)hydro phenanthrene skeleton
    • C07J41/0016—Oximes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J41/00—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring
    • C07J41/0033—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring not covered by C07J41/0005
    • C07J41/0038—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring not covered by C07J41/0005 with an androstane skeleton, including 18- or 19-substituted derivatives, 18-nor derivatives and also derivatives where position 17-beta is substituted by a carbon atom not directly bonded to a further carbon atom and not being part of an amide group
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/806—Fertility tests
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/906—Fertility tests

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Endocrinology (AREA)
  • Steroid Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SE DESCRIBEN UN METODO Y UN KIT PARA MEDIR ESTEROIDES POR MEDIO DE UN INMUNOENSAYO DE COMPETENCIA, PREFERENTEMENTE UN INMUNOENSAYO ENZIMATICO COMPETITIVO. EL METODO Y EL KIT IMPLICAN EL USO DE UN ANALOGO DE ESTEROIDE, CONJUGADO A UN MARCADOR. LOS ESTEROIDES QUE SON SUSCEPTIBLES DE SER DETECTADOS POR EL METODO Y EL KIT DE LA PRESENTE INVENCION INCLUYEN EL ESTRADIOL Y LA PROGESTERONA. EL METODO COMPRENDE LOS PASOS DE: A) INCUBACION DE UNA MEZCLA DE UNA MUESTRA PROBLEMA DE LA QUE SE SOSPECHE QUE CONTENGA UN DETERMINADO ESTEROIDE, UNA FASE SOLIDA ACOPLADA A UN ANTICUERPO ESPECIFICO PARA DICHO ESTEROIDE, Y UN CONJUGADO DE UN ANALOGO DE DICHO ESTEROIDE, PARA FORMAR COMPLEJOS ESTEROIDE / ANTICUERPO Y COMPLEJOS CONJUGADO / ANTICUERPO SOBRE DICHA FASE SOLIDA; B) SEPARACION DE DICHA FASE SOLIDA Y DICHA MEZCLA; C) MEDICION DE LA CANTIDAD DE MARCADOR PRESENTE EN DICHA MEZCLA O EN DICHA FASE SOLIDA; Y D) DETERMINACION DE LA CANTIDAD DE ESTEROIDE EN DICHA MUESTRA A PARTIR DE LA CANTIDAD DE MARCADOR. EL KIT CONTIENE UNA FASE SOLIDA ACOPLADA A UN ANTICUERPO ESPECIFICO PARA UN ESTEROIDE Y UN CONJUGADO DE UN ANALOGO DE DICHO ESTEROIDE.
ES96908511T 1995-03-03 1996-02-26 Deteccion de esteroides por medio de un inmunoensayo competitivo. Expired - Lifetime ES2176445T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/398,226 US5663054A (en) 1995-03-03 1995-03-03 Determination of steroids by competitive immunoassay

Publications (1)

Publication Number Publication Date
ES2176445T3 true ES2176445T3 (es) 2002-12-01

Family

ID=23574524

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96908511T Expired - Lifetime ES2176445T3 (es) 1995-03-03 1996-02-26 Deteccion de esteroides por medio de un inmunoensayo competitivo.

Country Status (8)

Country Link
US (3) US5663054A (es)
EP (1) EP0813684B1 (es)
JP (1) JP3640970B2 (es)
AT (1) ATE216780T1 (es)
CA (1) CA2214085A1 (es)
DE (1) DE69620876T2 (es)
ES (1) ES2176445T3 (es)
WO (1) WO1996027800A1 (es)

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US5854009A (en) * 1995-09-19 1998-12-29 Immuna Care Corporation Method of detecting estrogen-sensitive pathologies
US5977319A (en) * 1996-10-21 1999-11-02 Cambridge Antibody Technology Limited Specific binding members for estradiol; materials and methods
US6300082B1 (en) * 1998-03-20 2001-10-09 Ia, Inc. Method and apparatus for measurement of the effect of test compounds on signal transduction at the level of biological receptors
CA2283597C (en) 1998-10-02 2008-02-05 Ortho-Clinical Diagnostics, Inc. Reduced cortisol conjugates
JP2005503530A (ja) * 1999-09-10 2005-02-03 バイエックス，インコーポレイティド ホルモン補充療法のモニタリング
US6174665B1 (en) 1999-09-10 2001-01-16 Biex, Inc. Hormone replacement therapy monitoring
US7410807B2 (en) * 2000-07-24 2008-08-12 D Aurora Vito J Pregnancy and sex identification test based on saliva or other bodily fluids
US20030124618A1 (en) * 2001-12-04 2003-07-03 Lattec I/S Device for analysing analyte compounds and use hereof
JP3637392B2 (ja) * 2002-04-08 2005-04-13 独立行政法人産業技術総合研究所 燃料電池
AU2003278744B2 (en) 2002-08-28 2010-07-29 Harbor Biosciences, Inc. Therapeutic treatment methods
US20060068501A1 (en) * 2004-09-30 2006-03-30 Nj International Llc Apparatus and methods for steroid hormone testing
AU2007207242B2 (en) 2006-01-19 2012-08-02 Lattec I/S Dry stick device and method for determining an analyte in a sample
DK1982182T3 (da) * 2006-01-19 2012-05-14 Lattec I S Hidtil ukendt tør-stick-indretningskonstruktion og fremgangsmåde til bestemmelse af en analyt i en prøve under anvendelse af denne tør-stick-indretning
US20100105071A1 (en) * 2007-02-28 2010-04-29 Children's Medical Center Corporation Methods for predicting the onset of menarche
JP5701666B2 (ja) * 2011-04-14 2015-04-15 株式会社Ｌｓｉメディエンス 高親和性抗ハプテンポリクローナル抗体の作製法
WO2013025532A2 (en) * 2011-08-12 2013-02-21 Siemens Healthcare Diagnostics Inc. Detection of sex steroids
JP6221466B2 (ja) * 2013-07-29 2017-11-01 東ソー株式会社 ハプテンの測定方法
GB201317458D0 (en) * 2013-10-02 2013-11-13 Spd Swiss Prec Diagnostics Gmbh Improved pregnancy test device and method
WO2016014162A1 (en) 2014-07-25 2016-01-28 Becton, Dickinson And Company Analyte test strip assays, and test strips and kits for use in practicing the same
US10794920B2 (en) 2015-04-02 2020-10-06 Spd Swiss Precision Diagnostics Gmbh Pregnancy test device and method
EA201992612A1 (ru) 2017-06-05 2020-05-20 Обсева С.А. Схемы применения антагониста гонадотропин-высвобождающего гормона для лечения эндометриоза

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4282325A (en) * 1971-05-14 1981-08-04 Syva Company Enzyme bound corticosteroids
US4275149A (en) * 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
JPS60260592A (ja) * 1984-06-08 1985-12-23 Teikoku Hormone Mfg Co Ltd ステロイド測定用抗原
EP0200960A1 (en) * 1985-05-08 1986-11-12 Abbott Laboratories Total estriol fluorescence polarization immunoassay
US4857652A (en) * 1986-07-17 1989-08-15 Board Of Governors Of Wayne State University Chemiluminescent 1,2-dioxetane compounds
US4962192A (en) * 1986-07-17 1990-10-09 Board Of Governors Of Wayne State University Chemiluminescent 1,2-dioxetane compounds
EP0275260B1 (en) * 1986-07-24 1993-11-18 Tropix, Inc. Method of detecting a substance using enzymatically-induced decomposition of dioxetanes
US4990443A (en) * 1988-01-25 1991-02-05 Boehringer Mannheim Gmbh Hapten-protein conjugates and methods of use in immunoassays
FR2627588A1 (fr) * 1988-02-24 1989-08-25 Ire Medgenix Sa Procede de dosage immunologique d'une substance de structure haptenique
JPH02213766A (ja) * 1989-02-15 1990-08-24 Mochida Pharmaceut Co Ltd 免疫測定用試薬および免疫測定器具
GB8926407D0 (en) * 1989-11-22 1990-01-10 Gen Electric Plc Polymers
US5342760A (en) * 1992-06-10 1994-08-30 Abbott Laboratories Determination of estradiol by competitive immunoassay
US5395938A (en) * 1992-08-21 1995-03-07 Nichols Institute Diagnostics Biotinylated chemiluminescent labels and their conjugates, assays and assay kits
US5491071A (en) * 1993-08-03 1996-02-13 Abbott Laboratories Reagents and methods for the detection and quantification of testosterone in fluid samples

Also Published As

Publication number Publication date
EP0813684A1 (en) 1997-12-29
JP3640970B2 (ja) 2005-04-20
ATE216780T1 (de) 2002-05-15
CA2214085A1 (en) 1996-09-12
US6201141B1 (en) 2001-03-13
US5663054A (en) 1997-09-02
US5932431A (en) 1999-08-03
EP0813684B1 (en) 2002-04-24
DE69620876D1 (de) 2002-05-29
DE69620876T2 (de) 2002-12-12
WO1996027800A1 (en) 1996-09-12
JPH11501397A (ja) 1999-02-02

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