ES2173897T3 - Procedimiento para la clonacion especifica de acidos nucleicos. - Google Patents

Procedimiento para la clonacion especifica de acidos nucleicos.

Info

Publication number
ES2173897T3
ES2173897T3 ES94114675T ES94114675T ES2173897T3 ES 2173897 T3 ES2173897 T3 ES 2173897T3 ES 94114675 T ES94114675 T ES 94114675T ES 94114675 T ES94114675 T ES 94114675T ES 2173897 T3 ES2173897 T3 ES 2173897T3
Authority
ES
Spain
Prior art keywords
nucleic acids
procedure
specific cloning
ligated
cloning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94114675T
Other languages
English (en)
Inventor
Wolf Dr Bertling
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Original Assignee
November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin filed Critical November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Application granted granted Critical
Publication of ES2173897T3 publication Critical patent/ES2173897T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

Abstract

LA INVENCION SE REFIERE A UN PROCEDIMIENTO PARA LA CLONACION DE ACIDOS NUCLEICOS, CARACTERIZADO PORQUE DOS ELEMENTOS PRIMARIOS DE DOBLE MADEJA, PARCIALMENTE COMPLEMENTARIOS SON LIGADOS AL EXTREMO 3'' DE UNA MADEJA DNA INDIVIDUAL. LA SEGUNDA MADEJA SE SINTETIZA POR MEDIO DE UNA POLIMERASA DNA DONDE EL PRIMER ELEMENTO UTILIZADO ES AL MENOS PARCIALMENTE COMPLEMENTARIO CON EL OLIGONUCLEOTIDO AL QUE ESTA LIGADO. LA LINEA DNA ASI OBTENIDA PUEDE SER AMPLIFICADA ENTONCES CON DOS OLIGONUCLEOTIDOS ESPECIFICOS.
ES94114675T 1993-09-24 1994-09-17 Procedimiento para la clonacion especifica de acidos nucleicos. Expired - Lifetime ES2173897T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4332463A DE4332463A1 (de) 1993-09-24 1993-09-24 Verfahren zur spezifischen Klonierung von Nukleinsäuren

Publications (1)

Publication Number Publication Date
ES2173897T3 true ES2173897T3 (es) 2002-11-01

Family

ID=6498466

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94114675T Expired - Lifetime ES2173897T3 (es) 1993-09-24 1994-09-17 Procedimiento para la clonacion especifica de acidos nucleicos.

Country Status (6)

Country Link
EP (1) EP0645449B1 (es)
JP (1) JPH07177885A (es)
AT (1) ATE214096T1 (es)
DE (2) DE4332463A1 (es)
DK (1) DK0645449T3 (es)
ES (1) ES2173897T3 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2206451C (en) * 1994-12-09 2002-11-26 Wakunaga Seiyaku Kabushiki Kaisha Method for suppressing nonspecific hybridization in primer extension method
US5753462A (en) * 1995-06-07 1998-05-19 Zymogenetics, Inc. Secretion leader trap cloning method
JPH11506939A (ja) * 1995-06-07 1999-06-22 ザイモジェネティクス，インコーポレイティド 分泌リーダーのトラップクローニング法
DE19601385A1 (de) * 1996-01-16 1997-07-17 Mueller Manfred W Verfahren zur Amplifikation von Nukleinsäure
EP0981535A4 (en) * 1997-05-12 2000-11-29 Life Technologies Inc PROCESSES FOR PRODUCING AND PURIFYING NUCLEIC ACID MOLECULES
US6399334B1 (en) 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
US6368834B1 (en) * 1999-04-06 2002-04-09 Genome Technologies, Llc PCR genome walking with synthetic primer
US6083727A (en) * 1999-06-30 2000-07-04 Incyte Pharmaceuticals Inc. Methods and compositions for producing 5' enriched cDNA libraries
EP1325118B1 (en) * 2000-10-05 2016-05-25 Riken Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers.

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4666839A (en) * 1982-12-01 1987-05-19 Amgen Methods and materials for obtaining microbial expression of polypeptides including bovine prolactin
DE3929030A1 (de) * 1989-09-01 1991-03-07 Boehringer Mannheim Gmbh Verfahren zur vermehrung von nukleinsaeuren
GB9011454D0 (en) * 1990-05-22 1990-07-11 Medical Res Council Polynucleotide amplification
FR2678639B1 (fr) * 1991-07-03 1993-09-17 Rhone Poulenc Rorer Sa Procede de clonage d'acides nucleiques.

Also Published As

Publication number Publication date
DK0645449T3 (da) 2002-05-21
JPH07177885A (ja) 1995-07-18
EP0645449B1 (de) 2002-03-06
ATE214096T1 (de) 2002-03-15
DE59410068D1 (de) 2002-04-11
EP0645449A1 (de) 1995-03-29
DE4332463A1 (de) 1995-03-30

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