ES2169734T3 - Crioconservacion de equivalentes de tejidos cultivados. - Google Patents

Crioconservacion de equivalentes de tejidos cultivados.

Info

Publication number
ES2169734T3
ES2169734T3 ES94927359T ES94927359T ES2169734T3 ES 2169734 T3 ES2169734 T3 ES 2169734T3 ES 94927359 T ES94927359 T ES 94927359T ES 94927359 T ES94927359 T ES 94927359T ES 2169734 T3 ES2169734 T3 ES 2169734T3
Authority
ES
Spain
Prior art keywords
cultured tissue
equivalent
vitro
cryoprotectant solution
cultured
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94927359T
Other languages
English (en)
Inventor
Leon Wilkins
Stephen Watson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Organogenesis Inc
Original Assignee
Organogenesis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Organogenesis Inc filed Critical Organogenesis Inc
Application granted granted Critical
Publication of ES2169734T3 publication Critical patent/ES2169734T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents

Abstract

LA INVENCION SE DIRIGE A LA CRIOCONSERVACION DE EQUIVALENTES DE TEJIDOS CULTIVADOS HECHOS MEDIANTE TECNOLOGIA IN VITRO. LA INVENCION COMPRENDE LA INMERSION DEL EQUIVALENTE DEL TEJIDO CULTIVADO EN UNA SOLUCION CRIOPROTECTORA ENFRIADA, LA AGITACION DE LA SOLUCION CRIOPROTECTORA Y DEL TEJIDO CULTIVADO SUMERGIDO PARA CONSEGUIR UNA PENETRACION EFECTIVA DE LA SOLUCION CRIOPROTECTORA EN EL INTERIOR DEL TEJIDO CULTIVADO, Y LA CONGELACION POSTERIOR DEL TEJIDO CULTIVADO A UNA ALTA VELOCIDAD DE CONGELACION. EL EQUIVALENTE DEL TEJIDO CULTIVADO, CRIOCONSERVADO, PUEDE ALMACENARSE DURANTE PERIODOS INDEFINIDOS DE TIEMPO ANTES DE SU USO. EL EQUIVALENTE DEL TEJIDO CONGELADO ES UN MODELO IN VITRO DEL TEJIDO HUMANO EQUIVALENTE, QUE, CUANDO SE RETIRA DEL ALMACENAMIENTO, PUEDE UTILIZARSE PARA TRASPLANTE O IMPLANTE IN VIVO O PARA LA SELECCION DE COMPUESTOS IN VITRO.
ES94927359T 1993-09-15 1994-09-14 Crioconservacion de equivalentes de tejidos cultivados. Expired - Lifetime ES2169734T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/121,377 US5518878A (en) 1993-09-15 1993-09-15 Cryopreservation of cultured skin or cornea equivalents with agitation

Publications (1)

Publication Number Publication Date
ES2169734T3 true ES2169734T3 (es) 2002-07-16

Family

ID=22396314

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94927359T Expired - Lifetime ES2169734T3 (es) 1993-09-15 1994-09-14 Crioconservacion de equivalentes de tejidos cultivados.

Country Status (16)

Country Link
US (1) US5518878A (es)
EP (1) EP0719084B1 (es)
JP (1) JPH09505032A (es)
KR (1) KR100361357B1 (es)
AT (1) ATE210373T1 (es)
AU (1) AU683301B2 (es)
CA (1) CA2182814C (es)
CY (1) CY2364B1 (es)
DE (1) DE69429436T2 (es)
DK (1) DK0719084T3 (es)
ES (1) ES2169734T3 (es)
FI (1) FI113833B (es)
NO (1) NO311631B1 (es)
NZ (1) NZ273514A (es)
PT (1) PT719084E (es)
WO (1) WO1995007611A1 (es)

Families Citing this family (38)

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US5723282A (en) * 1991-07-08 1998-03-03 The American National Red Cross Method of preparing organs for vitrification
US5891617A (en) * 1993-09-15 1999-04-06 Organogenesis Inc. Cryopreservation of harvested skin and cultured skin or cornea equivalents by slow freezing
ATE206583T1 (de) 1994-11-09 2001-10-15 Celadon Science Llc Wundbesserungsverband und verfahren zur dessen konservierung
US20040002772A1 (en) * 1995-04-28 2004-01-01 Organogenesis, Inc. Tissue equivalents with perforations for improved integration to host tissues and methods for producing perforated tissue equivalents
US5689961A (en) 1996-01-30 1997-11-25 Organogenesis Inc. Ice seeding apparatus for cryopreservation systems
US6630001B2 (en) * 1998-06-24 2003-10-07 International Heart Institute Of Montana Foundation Compliant dehyrated tissue for implantation and process of making the same
AU6424799A (en) * 1998-10-14 2000-05-01 Katrina T. Forest Method for vitrification of a biological specimen
AU2004200933B2 (en) * 1998-10-14 2005-08-11 Katrina T Forest Method for vitrification of a biological specimen
US6403376B1 (en) * 1998-11-16 2002-06-11 General Hospital Corporation Ultra rapid freezing for cell cryopreservation
US6300130B1 (en) * 1998-11-16 2001-10-09 The General Hospital Corporation And University Of Massachusetts Ultra rapid freezing for cell cryopreservation
CN1333818A (zh) 1998-11-19 2002-01-30 奥加诺吉尼西斯公司 生物工程化的组织构建物、其制备方法及其应用
US6358739B1 (en) 1999-04-12 2002-03-19 Modex Therapeutiques, S.A. Transiently immortalized cells
US6194137B1 (en) * 1999-04-13 2001-02-27 Organ Recovery Systems, Inc. Method of cryopreservation of blood vessels by vitrification
US6352708B1 (en) 1999-10-14 2002-03-05 The International Heart Institute Of Montana Foundation Solution and method for treating autologous tissue for implant operation
US6878543B1 (en) 1999-10-25 2005-04-12 Nsgene Sa Cultures of GFAP+ nestin+ cells that differentiate to neurons
KR20010008306A (ko) * 2000-11-22 2001-02-05 김진경 저온처리 후 조직 배양 방법
DE10061968A1 (de) * 2000-12-13 2002-06-20 Friedrich Hoffmann Verfahren und Vorrichtung zum Einfrieren von Hornhautgewebe
JP2004520828A (ja) 2000-12-27 2004-07-15 オーテック・インターナショナル，インコーポレイテッド 凍結保存された複合生体構築物の製造方法および該方法から得られる製造物
DE10151296A1 (de) 2001-10-17 2003-04-30 Boehringer Ingelheim Pharma Keratinozyten verwendbar als biologisch aktive Substanz bei der Behandlung von Wunden
US20030096412A1 (en) * 2001-11-20 2003-05-22 Cassell Allan J. Method of preparing biological materials for cryopreservation using pre-chilled protectant
PL212661B1 (pl) 2002-05-16 2012-11-30 Absorber Sposób izolacji komórki prekursorowej sródblonka
JP5100078B2 (ja) 2006-10-05 2012-12-19 株式会社セルバンク 組織由来細胞の凍結保存方法
CN101939631A (zh) * 2008-01-17 2011-01-05 库克泌尿科学公司万斯产品公司 用于玻璃化的快速冷却设备
CN102438445B (zh) * 2009-02-23 2014-12-10 细胞与组织系统股份有限公司 组织的无冰低温贮藏法
KR101900116B1 (ko) 2010-09-01 2018-09-18 리전츠 오브 더 유니버스티 오브 미네소타 개선된 이식가능성을 위한 조직 또는 장기의 재세포화 방법
JP6012164B2 (ja) 2011-11-25 2016-10-25 住友化学株式会社 多能性幹細胞由来の組織の凍結保存方法
JP5915977B2 (ja) * 2011-11-29 2016-05-11 学校法人明治大学 凍結細胞シートの製造方法
WO2013174769A1 (en) 2012-05-25 2013-11-28 Boehringer Ingelheim International Gmbh Use of keratinocytes as a biologically active substance in the treatment of wounds, such as diabetic wounds, optionally in combination with a dpp-4 inhibitor
TW201422155A (zh) * 2012-07-06 2014-06-16 Arigos Biomedical Inc 以氣體灌注快速冷卻與升溫預防玻璃質化組織受熱-力破壞的方法與裝置
CA2880808C (en) 2012-08-01 2023-01-24 United Therapeutics Corporation Treatment of pulmonary arterial hypertension with mesenchymal stem cells
EP3492084B1 (en) 2012-08-01 2021-02-17 United Therapeutics Corporation Treatment of pulmonary arterial hypertension with prostacyclin-treated endothelial progenitor cells
KR101480806B1 (ko) 2012-09-19 2015-01-09 한국생산기술연구원 스캐폴드를 이용한 세포 또는 조직 동결 방법, 및 세포 또는 조직 동결용 키트
ES2697573T3 (es) 2013-01-09 2019-01-25 United Therapeutics Corp Tratamiento de la vasculopatía con prostaciclina y células madre mesenquimatosas
CA3071928A1 (en) * 2013-03-13 2014-10-02 Stratatech Corporation Cryopreservation of viable human skin substitutes
CA2917109C (en) * 2013-07-02 2022-11-01 Walter H. Guenzburg A method of freeze-drying encapsulated cells, freeze-dried encapsulated cells, compositions containning freeze-dried encapsulated cells and uses of such cells and compositions
EP3274007B1 (en) 2015-03-26 2020-09-30 Miromatrix Medical Inc. Gas filled decellularized extracellular matrix
CA3041514A1 (en) 2016-10-24 2018-05-03 United Therapeutics Corporation Enhancement of msc immunomodulatory properties by treprostinil
CN113016776A (zh) * 2019-12-24 2021-06-25 上海明悦医疗科技有限公司 载体、抽真空装置与组织冻存系统

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4539716A (en) * 1981-03-19 1985-09-10 Massachusetts Institute Of Technology Fabrication of living blood vessels and glandular tissues
US4546500A (en) * 1981-05-08 1985-10-15 Massachusetts Institute Of Technology Fabrication of living blood vessels and glandular tissues
US4485096A (en) * 1982-02-26 1984-11-27 Massachusetts Institute Of Technology Tissue-equivalent and method for preparation thereof
US4485097A (en) * 1982-05-26 1984-11-27 Massachusetts Institute Of Technology Bone-equivalent and method for preparation thereof
US5084377A (en) * 1984-09-19 1992-01-28 Larry Rowan Cryogenic suspension method
US4604346A (en) * 1984-10-09 1986-08-05 Massachusetts Institute Of Technology Skin-equivalent prepared by the use of punch biopsy
IT1207525B (it) * 1987-06-23 1989-05-25 Ist Naz Ric Sul Cancro Metodo per la preservazione difogli trapiantabili di epitelio coltivato in vitro vitale.
US4837379A (en) * 1988-06-02 1989-06-06 Organogenesis Inc. Fibrin-collagen tissue equivalents and methods for preparation thereof
US5145770A (en) * 1990-06-04 1992-09-08 Biosurface Technology, Inc. Cryopreservation of cultured epithelial sheets

Also Published As

Publication number Publication date
NO311631B1 (no) 2001-12-27
KR100361357B1 (ko) 2004-12-30
EP0719084A1 (en) 1996-07-03
NO961048D0 (no) 1996-03-14
FI113833B (fi) 2004-06-30
PT719084E (pt) 2002-05-31
FI961124A0 (fi) 1996-03-11
DE69429436D1 (de) 2002-01-24
EP0719084B1 (en) 2001-12-12
CA2182814C (en) 1999-02-02
CY2364B1 (en) 2004-06-04
KR960704461A (ko) 1996-10-09
CA2182814A1 (en) 1995-03-23
EP0719084A4 (en) 1998-06-10
WO1995007611A1 (en) 1995-03-23
US5518878A (en) 1996-05-21
JPH09505032A (ja) 1997-05-20
NZ273514A (en) 1997-10-24
FI961124A (fi) 1996-03-15
AU683301B2 (en) 1997-11-06
AU7683194A (en) 1995-04-03
DK0719084T3 (da) 2002-04-08
ATE210373T1 (de) 2001-12-15
NO961048L (no) 1996-03-14
DE69429436T2 (de) 2002-07-18

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