ES2167885T3 - Aparato y metodo de formacion de imagen por microscopia. - Google Patents

Aparato y metodo de formacion de imagen por microscopia.

Info

Publication number
ES2167885T3
ES2167885T3 ES98914965T ES98914965T ES2167885T3 ES 2167885 T3 ES2167885 T3 ES 2167885T3 ES 98914965 T ES98914965 T ES 98914965T ES 98914965 T ES98914965 T ES 98914965T ES 2167885 T3 ES2167885 T3 ES 2167885T3
Authority
ES
Spain
Prior art keywords
microscopy
image formation
images
recorded
transparent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES98914965T
Other languages
English (en)
Inventor
Tony Wilson
Mark Andrew Aquilla Neil
Rimvydas Juskaitis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford University Innovation Ltd
Original Assignee
Oxford University Innovation Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9706843.1A external-priority patent/GB9706843D0/en
Priority claimed from GBGB9726485.7A external-priority patent/GB9726485D0/en
Application filed by Oxford University Innovation Ltd filed Critical Oxford University Innovation Ltd
Application granted granted Critical
Publication of ES2167885T3 publication Critical patent/ES2167885T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G02B21/0044—Scanning details, e.g. scanning stages moving apertures, e.g. Nipkow disks, rotating lens arrays
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/50—Depth or shape recovery
    • G06T7/521—Depth or shape recovery from laser ranging, e.g. using interferometry; from the projection of structured light
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/50—Depth or shape recovery
    • G06T7/55—Depth or shape recovery from multiple images

Landscapes

  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Multimedia (AREA)
  • Theoretical Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Closed-Circuit Television Systems (AREA)
  • Eye Examination Apparatus (AREA)

Abstract

Un método de generación de una imagen de una muestra (0) que consta de los pasos de iluminar la muestra (0) con una fuente de luz (10); generar un modelo espacial sustancialmente periódico sobre la muestra (0); grabar una primera imagen de la muestra (0); alterar la fase espacial del modelo sobre la muestra (0) y grabar una segunda imagen de la muestra (0); repetir al menos una vez más los pasos de alterar la fase espacial del modelo en la muestra (0) y grabar una tercera imagen de la muestra (0), siendo diferente la fase espacial del modelo en al menos tres imágenes grabadas de la muestra (0); y analizar las tres o más imágenes grabadas de la muestra (0) para eliminar el modelo espacial de las imágenes para generar con ello una imagen ópticamente seccionada de la muestra (0), caracterizado porque se obtiene una imagen ópticamente seccionada agrupando las imágenes grabadas en parejas de imágenes, calculando la raíz cuadrada de la suma de los cuadrados de las diferencias entre las imágenes grabadas en cada pareja de imágenes, y, si se graban tres imágenes, el modelo espacial se elimina según la fórmula: I= q (I1-I2) 2 + (I1-I3) 2 + (I2-I3) 2 en la que las tres imágenes grabadas son I1, I2 e I3, y la imagen ópticamente seccionada es I.
ES98914965T 1997-04-04 1998-04-03 Aparato y metodo de formacion de imagen por microscopia. Expired - Lifetime ES2167885T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9706843.1A GB9706843D0 (en) 1997-04-04 1997-04-04 Microscopy imaging apparatus and a method thereof
GBGB9726485.7A GB9726485D0 (en) 1997-12-15 1997-12-15 Microscopy imaging apparatus and method

Publications (1)

Publication Number Publication Date
ES2167885T3 true ES2167885T3 (es) 2002-05-16

Family

ID=26311316

Family Applications (1)

Application Number Title Priority Date Filing Date
ES98914965T Expired - Lifetime ES2167885T3 (es) 1997-04-04 1998-04-03 Aparato y metodo de formacion de imagen por microscopia.

Country Status (11)

Country Link
US (1) US6376818B1 (es)
EP (1) EP0972220B1 (es)
JP (1) JP3066874B2 (es)
KR (1) KR100504261B1 (es)
AT (1) ATE208911T1 (es)
AU (1) AU737617B2 (es)
DE (1) DE69802514T2 (es)
DK (1) DK0972220T3 (es)
ES (1) ES2167885T3 (es)
GB (1) GB2338858B (es)
WO (1) WO1998045745A1 (es)

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Also Published As

Publication number Publication date
ATE208911T1 (de) 2001-11-15
WO1998045745A1 (en) 1998-10-15
JP2000506634A (ja) 2000-05-30
JP3066874B2 (ja) 2000-07-17
GB9922732D0 (en) 1999-11-24
US6376818B1 (en) 2002-04-23
DE69802514T2 (de) 2002-06-27
GB2338858B (en) 2000-12-27
DK0972220T3 (da) 2002-03-11
AU737617B2 (en) 2001-08-23
EP0972220B1 (en) 2001-11-14
GB2338858A (en) 1999-12-29
AU6926498A (en) 1998-10-30
DE69802514D1 (de) 2001-12-20
KR20010005974A (ko) 2001-01-15
EP0972220A1 (en) 2000-01-19
KR100504261B1 (ko) 2005-07-27

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