ES2152913T1 - Metodo de analisis cualitativo y cuantitativo de las poblaciones microbianas potencialmente presentes en una muestra. - Google Patents
Metodo de analisis cualitativo y cuantitativo de las poblaciones microbianas potencialmente presentes en una muestra.Info
- Publication number
- ES2152913T1 ES2152913T1 ES98952692T ES98952692T ES2152913T1 ES 2152913 T1 ES2152913 T1 ES 2152913T1 ES 98952692 T ES98952692 T ES 98952692T ES 98952692 T ES98952692 T ES 98952692T ES 2152913 T1 ES2152913 T1 ES 2152913T1
- Authority
- ES
- Spain
- Prior art keywords
- sample
- qualitative
- quantitative analysis
- potentially
- microbial populations
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Método de análisis cualitativo y cuantitativo de la o las poblaciones microbianas potencialmente presentes en una muestra, caracterizado porque cosiste en: - poner en contacto los microorganismos potencialmente presentes en dicha muestra con como mínimo una sonda de oligonucleótido dirigida a ARN, en lo sucesivo denominada sonda específica, capaz de dirigirse a una población microbiológica deseada, bajo condiciones favorables para hibridación in situ en células completas, - extraer las sondas hibridadas por separación de su diana y elución fuera de dichas células, - detectar las sondas extraídas y medir la cantidad de las mismas o sus cantidades respectivas.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9712552A FR2769323B1 (fr) | 1997-10-08 | 1997-10-08 | Moyens pour l'analyse qualitative et quantitative des populations microbiennes eventuellement presentes dans un echantillon |
FR9712552 | 1997-10-08 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2152913T1 true ES2152913T1 (es) | 2001-02-16 |
ES2152913T3 ES2152913T3 (es) | 2005-02-01 |
Family
ID=9511969
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES98952692T Expired - Lifetime ES2152913T3 (es) | 1997-10-08 | 1998-10-02 | Medio para el analisis cualitativo y cuantitativo de las poblaciones microbianas potencialmente presentes en una muestra. |
Country Status (9)
Country | Link |
---|---|
US (1) | US6808879B1 (es) |
EP (1) | EP1042502B1 (es) |
JP (1) | JP2001519168A (es) |
AU (1) | AU732150B2 (es) |
CA (1) | CA2305573A1 (es) |
DE (2) | DE1042502T1 (es) |
ES (1) | ES2152913T3 (es) |
FR (1) | FR2769323B1 (es) |
WO (1) | WO1999018234A2 (es) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2369577A1 (en) | 1999-05-07 | 2000-11-16 | Vermicon Ag | Method for detecting microorganisms in a sample |
DE10022304B4 (de) * | 2000-05-08 | 2004-01-15 | Vermicon Ag | Verfahren zur Identifizierung, Quantifizierung und Visualisierung von Mikroorganismen |
DE10128400A1 (de) * | 2001-06-12 | 2002-12-19 | Vermicon Ag | Verfahren zum spezifischen Schnellnachweis fadenförmiger Bakterien |
US8048623B1 (en) | 2002-04-24 | 2011-11-01 | The University Of North Carolina At Greensboro | Compositions, products, methods and systems to monitor water and other ecosystems |
US7214492B1 (en) | 2002-04-24 | 2007-05-08 | The University Of North Carolina At Greensboro | Nucleic acid arrays to monitor water and other ecosystems |
US8383342B2 (en) | 2002-04-24 | 2013-02-26 | The University Of North Carolina At Greensboro | Compositions, products, methods and systems to monitor water and other ecosystems |
US9126165B1 (en) | 2002-04-24 | 2015-09-08 | The University Of North Carolina At Greensboro | Nucleic acid arrays to monitor water and other ecosystems |
JP4568879B2 (ja) * | 2004-05-19 | 2010-10-27 | 独立行政法人産業技術総合研究所 | 土壌診断用バイオセンサおよびこれを用いた土壌診断法 |
DE102004025710A1 (de) * | 2004-05-26 | 2005-12-22 | Eppendorf Ag | Verfahren zur taxonspezifischen Zellidentifizierung und Zellsortierung auf grampositive Bakterien und Vorrichtungen hierfür |
US7771941B2 (en) * | 2006-06-23 | 2010-08-10 | University Of South Florida | Method for determining the specific growth rate of distinct microbial populations in a non-homogeneous system |
AU2015264073B2 (en) * | 2014-05-22 | 2021-08-12 | Aobiome Llc | Methods of preparing materials with ammonia oxidizing bacteria and testing materials for ammonia oxidizing bacteria |
US11608516B2 (en) * | 2015-04-15 | 2023-03-21 | Ecolab Usa Inc. | Method for determination of diversity and viability thresholds used to assess microorganisms in process samples |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8627738D0 (en) | 1986-11-20 | 1987-01-21 | Dallimer D S | Silos |
JP3116353B2 (ja) * | 1986-11-24 | 2000-12-11 | ジエン‐プローブ・インコーポレイテツド | 非ウイルス微生物の検出及び/又は定量用核酸プローブ |
US5403711A (en) * | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
AU6075490A (en) * | 1989-07-11 | 1991-02-06 | Microprobe Corporation | Quantification of bacteria using a nucleic acid hybridization assay |
CA2074214C (en) * | 1991-07-23 | 2000-02-22 | Will Bloch | Improvements in the in situ pcr |
US5426025A (en) * | 1992-05-28 | 1995-06-20 | Florida State University | Species-specific DNA probes for vibrio vulnificus methods and kits |
WO1996019585A1 (en) * | 1994-12-20 | 1996-06-27 | Heidelberg Repatriation Hospital | Typing of microorganisms |
JP4040087B2 (ja) * | 1995-05-05 | 2008-01-30 | アプレラ コーポレイション | Pcr増幅とハイブリダイゼーションプロービングアッセイとを組み合わせた方法およびそのための試薬 |
DE69717601T2 (de) * | 1996-01-26 | 2003-09-18 | Abbott Lab | Verfahren zur analyse von nukleinsäure-wiederholungssequenzen |
-
1997
- 1997-10-08 FR FR9712552A patent/FR2769323B1/fr not_active Expired - Fee Related
-
1998
- 1998-10-02 AU AU10294/99A patent/AU732150B2/en not_active Ceased
- 1998-10-02 ES ES98952692T patent/ES2152913T3/es not_active Expired - Lifetime
- 1998-10-02 EP EP98952692A patent/EP1042502B1/en not_active Expired - Lifetime
- 1998-10-02 WO PCT/EP1998/006286 patent/WO1999018234A2/en active IP Right Grant
- 1998-10-02 CA CA002305573A patent/CA2305573A1/en not_active Abandoned
- 1998-10-02 JP JP2000515026A patent/JP2001519168A/ja active Pending
- 1998-10-02 US US09/529,217 patent/US6808879B1/en not_active Expired - Fee Related
- 1998-10-02 DE DE1042502T patent/DE1042502T1/de active Pending
- 1998-10-02 DE DE69825783T patent/DE69825783T2/de not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
WO1999018234A2 (en) | 1999-04-15 |
FR2769323B1 (fr) | 2001-07-13 |
AU1029499A (en) | 1999-04-27 |
AU732150B2 (en) | 2001-04-12 |
JP2001519168A (ja) | 2001-10-23 |
WO1999018234A3 (en) | 2000-09-28 |
EP1042502B1 (en) | 2004-08-18 |
DE69825783D1 (de) | 2004-09-23 |
FR2769323A1 (fr) | 1999-04-09 |
DE1042502T1 (de) | 2001-06-07 |
CA2305573A1 (en) | 1999-04-15 |
EP1042502A1 (en) | 2000-10-11 |
ES2152913T3 (es) | 2005-02-01 |
US6808879B1 (en) | 2004-10-26 |
DE69825783T2 (de) | 2005-08-25 |
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