ES2141163T3 - Estabilizacion de sales de tetrazoilo en un reactivo. - Google Patents

Estabilizacion de sales de tetrazoilo en un reactivo.

Info

Publication number
ES2141163T3
ES2141163T3 ES93916919T ES93916919T ES2141163T3 ES 2141163 T3 ES2141163 T3 ES 2141163T3 ES 93916919 T ES93916919 T ES 93916919T ES 93916919 T ES93916919 T ES 93916919T ES 2141163 T3 ES2141163 T3 ES 2141163T3
Authority
ES
Spain
Prior art keywords
reagent
added
film
sample
fluid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93916919T
Other languages
English (en)
Inventor
Diana Faye Storhoff
David Tabb
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics Corp
Original Assignee
Roche Diagnostics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Corp filed Critical Roche Diagnostics Corp
Application granted granted Critical
Publication of ES2141163T3 publication Critical patent/ES2141163T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine

Abstract

UN REACTIVO ESTABLE PARA EL ENSAYO DE UNA SUSTANCIA DE ANALISIS A PARTIR DE UNA MUESTRA DE UN FLUIDO. EL REACTIVO INCLUYE UNA ENZIMA, UN MEDIADOR, UNA SAL DE TETRAZOLIO Y UN AGENTE OXIDANTE SELECCIONADO DEL GRUPO QUE CONSISTE EN CLORATO SODICO, 2,5-DIMETILHEXANO-2,5DIHIDROPEROXIDO, PEROXIDO DE BENZOILO, PEROXIDO DE T-BUTILO, YODATO SODICO, N-ETILMALEIMIDA, 7-BUTILPEROXIACETATO, ACETILACETONATO DE NIQUEL, CLORURO ESTANNICO, HIDRATO DE TRICLORURO DE RODIO (III) Y TBUTILPERBENZOATO. ES IMPORTANTE TENER EN CUENTA QUE LA ADICION DE UN AGENTE OXIDANTE DEL GRUPO ARRIBA ESPECIFICADO ESTABILIZA EL REACTIVO LO QUE PREVIENE UNA REACCION MUY VACIA (QUE DA UN ERROR EN EL ENSAYO DE UNA SUSTANCIA DE ANALISIS). EL REACTIVO SE PUEDE PRESENTAR A MODO DE SOLUCION SI SE AÑADE AGUA O SE PUEDE INCLUIR EN UNA PELICULA SI SE AÑADE UN AGENTE FORMADOR DE PELICULAS. EL REACTIVO SE PUEDE UTILIZAR PARA LLEVAR A CABO EL ENSAYO DE UNA SUSTANCIA DE ANALISIS A PARTIR DE UNA MUESTRA DE UN FLUIDO. EL ENSAYO SE PUEDE LLEVAR ACABO EN UN MEDIO EN SOLUCION SI SE AÑADE LA MUESTRA DEL FLUIDO AL REACTIVO LIQUIDO Y SI SE MIDE ESPECTROFOTOMETRICAMENTE LA ABSORBENCIA DEL FORMATO INDICATIVO DEL COLOR (PRODUCIDO MEDIANTE LA REDUCCION DE LA SAL DE TETRAZOLIO BAJO CONDICIONES DE ENSAYO). DE IGUAL MODO, EL ENSAYO SE PUEDE LLEVAR A CABO EN UN SOPORTE DE PELICULA SI SE AÑADE LA MUESTRA DEL FLUIDO EN EL REACTIVO QUE HAY INCORPORADO EN LA PELICULA Y SI SE MIDE ESPECTROFOTOMETRICAMENTE LA REFLECTANCIA O TRANSMITANCIA DEL FORMATO INDICADOR DEL COLOR.
ES93916919T 1992-07-02 1993-07-01 Estabilizacion de sales de tetrazoilo en un reactivo. Expired - Lifetime ES2141163T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US90812792A 1992-07-02 1992-07-02

Publications (1)

Publication Number Publication Date
ES2141163T3 true ES2141163T3 (es) 2000-03-16

Family

ID=25425242

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93916919T Expired - Lifetime ES2141163T3 (es) 1992-07-02 1993-07-01 Estabilizacion de sales de tetrazoilo en un reactivo.

Country Status (5)

Country Link
US (1) US5583006A (es)
EP (1) EP0648278B1 (es)
DE (1) DE69327242T2 (es)
ES (1) ES2141163T3 (es)
WO (1) WO1994001578A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6656697B1 (en) 1998-09-28 2003-12-02 Lifescan, Inc. Diagnostics based on tetrazolium compounds
US6720164B1 (en) 1999-03-19 2004-04-13 Sapporo Immuno Diagnostic Laboratory Method of determining substrate, and biosensor
US6635434B1 (en) 1999-09-17 2003-10-21 Exiqon A/S Immunoassay for pesticides and their degradation products
US6420128B1 (en) 2000-09-12 2002-07-16 Lifescan, Inc. Test strips for detecting the presence of a reduced cofactor in a sample and method for using the same
US6586199B2 (en) 2001-11-20 2003-07-01 Lifescan, Inc. Stabilized tetrazolium reagent compositions and methods for using the same
US6939685B2 (en) 2001-11-20 2005-09-06 Lifescan, Inc. Stabilized tetrazolium phenazine reagent compositions and methods for using the same
US6703216B2 (en) 2002-03-14 2004-03-09 The Regents Of The University Of California Methods, compositions and apparatuses for detection of gamma-hydroxybutyric acid (GHB)
US7955484B2 (en) * 2005-12-14 2011-06-07 Nova Biomedical Corporation Glucose biosensor and method
US8691135B2 (en) * 2011-06-06 2014-04-08 King Abdulaziz City for Science and Technology (KACST) Method of forming a film of novel composition for a dosimeter
CN111788472B (zh) 2018-03-07 2023-12-15 泰尔茂株式会社 用于测定生物试样中的分析对象物浓度的方法、试剂和芯片
CN116297435A (zh) * 2023-05-15 2023-06-23 北京索莱宝科技有限公司 乙酰乙酸含量检测试剂盒、其使用方法和检测装置

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1318568A (en) * 1970-09-17 1973-05-31 Miles Lab Colourimetric assay of dehydrogenases
US4042392A (en) * 1975-04-14 1977-08-16 Eastman Kodak Company Formazan images by physical development of catalytic metal nuclei image
US4247633A (en) * 1979-04-05 1981-01-27 Pabst Brewing Company Reagent for colorimetric determination of creative phosphokinase
DE3012368C2 (de) * 1980-03-29 1982-04-15 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und diagnostische Mittel zum Nachweis von Redox-Reaktionen
JPS5768798A (en) * 1980-10-14 1982-04-27 Toyo Jozo Co Ltd Novel measurement of amylase activity
NZ199380A (en) * 1981-12-23 1986-08-08 J R Baker Determination of serum glucose levels in blood samples
JPS5926061A (ja) * 1982-08-02 1984-02-10 Eiken Kagaku Kk 体液中の成分定量用試験片
US4748115A (en) * 1986-01-08 1988-05-31 Abbott Laboratories Substrate formulation in 2-amino-2-methyl-1-propanol buffer for alkaline phosphatase assays
US5036000A (en) * 1986-12-16 1991-07-30 Enzymatics, Inc. Threshold color control system
JPS63283600A (ja) * 1987-05-18 1988-11-21 Fuji Photo Film Co Ltd クレアチンキナ−ゼ活性測定用分析要素
US4892817A (en) * 1987-09-21 1990-01-09 Biogenex Laboratories Stable phosphatase substrate composition
IL85018A0 (en) * 1988-01-03 1988-06-30 Orgenics Ltd Stable chromogenic substrate mixture of indoxyl phosphate and tetrazolium salt,method of making and using same in biological and diagnostic assays
US5126247A (en) * 1988-02-26 1992-06-30 Enzymatics, Inc. Method, system and devices for the assay and detection of biochemical molecules
US5126275A (en) * 1990-09-19 1992-06-30 Miles Inc. Analytical method using tetrazolium salt indicators having a reflectance plateau

Also Published As

Publication number Publication date
EP0648278B1 (en) 1999-12-08
US5583006A (en) 1996-12-10
WO1994001578A1 (en) 1994-01-20
EP0648278A4 (en) 1997-05-07
DE69327242D1 (de) 2000-01-13
EP0648278A1 (en) 1995-04-19
DE69327242T2 (de) 2000-05-25

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