ES2133133B1 - Metodo e instalacion para la determinacion cuantitativa de hidroximetabolitos de la vitamina d3 de forma continua y automatica. - Google Patents

Metodo e instalacion para la determinacion cuantitativa de hidroximetabolitos de la vitamina d3 de forma continua y automatica.

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Publication number
ES2133133B1
ES2133133B1 ES9702577A ES9702577A ES2133133B1 ES 2133133 B1 ES2133133 B1 ES 2133133B1 ES 9702577 A ES9702577 A ES 9702577A ES 9702577 A ES9702577 A ES 9702577A ES 2133133 B1 ES2133133 B1 ES 2133133B1
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ES
Spain
Prior art keywords
vitamin
installation
liquid
hydroxymetabolites
quantitative determination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9702577A
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English (en)
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ES2133133A1 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ORTIZ BOYER FRANCISCA
Original Assignee
ORTIZ BOYER FRANCISCA
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Publication date
Application filed by ORTIZ BOYER FRANCISCA filed Critical ORTIZ BOYER FRANCISCA
Priority to ES9702577A priority Critical patent/ES2133133B1/es
Publication of ES2133133A1 publication Critical patent/ES2133133A1/es
Application granted granted Critical
Publication of ES2133133B1 publication Critical patent/ES2133133B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

Método e instalación para la determinación cuantitativa de hidroximetabolitos de la vitamina D{sub,3 de forma continua y automática. El método comprende: (a) extracción líquido-líquido manual de la muestra; (b) limpieza y preconcentración del extracto por extracción líquido-sólido; (c) separación-detección del extracto por cromatografía de líquidos con detección UV-visible, estando sincronizadas las etapas (b) y (c) por una unidad de control. La instalación comprende esencialmente una bomba peristáltica (BP), un muestreador automático (TM), una válvula de selección (SV1), una válvula de inyección (IV1) y una minicolumna de extracción (MC). Aplicación en el sector técnico de los métodos analíticos para la determinación de vitaminas y sus metabolitos, concretamente vitamina D{sub,3 .
ES9702577A 1997-12-11 1997-12-11 Metodo e instalacion para la determinacion cuantitativa de hidroximetabolitos de la vitamina d3 de forma continua y automatica. Expired - Fee Related ES2133133B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9702577A ES2133133B1 (es) 1997-12-11 1997-12-11 Metodo e instalacion para la determinacion cuantitativa de hidroximetabolitos de la vitamina d3 de forma continua y automatica.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9702577A ES2133133B1 (es) 1997-12-11 1997-12-11 Metodo e instalacion para la determinacion cuantitativa de hidroximetabolitos de la vitamina d3 de forma continua y automatica.

Publications (2)

Publication Number Publication Date
ES2133133A1 ES2133133A1 (es) 1999-08-16
ES2133133B1 true ES2133133B1 (es) 2000-05-01

Family

ID=8301472

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9702577A Expired - Fee Related ES2133133B1 (es) 1997-12-11 1997-12-11 Metodo e instalacion para la determinacion cuantitativa de hidroximetabolitos de la vitamina d3 de forma continua y automatica.

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ES (1) ES2133133B1 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102253130A (zh) * 2010-12-24 2011-11-23 天津天狮生物发展有限公司 测定复合维生素中维生素a、维生素d和维生素e的方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2334742B1 (es) * 2008-06-17 2011-02-09 Sanidad Y Residencias 21, S.A. Procedimiento automatizado para la determinacion simultanea de vitaminas liposolubles.

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61160055A (ja) * 1985-01-09 1986-07-19 Masaki Hasegawa 分離剤
SU1363060A1 (ru) * 1986-07-04 1987-12-30 Научно-исследовательский институт животноводства Лесостепи и Полесья УССР Способ количественного определени витамина Д @ в растительных кормах
JPS63158455A (ja) * 1986-12-22 1988-07-01 Takeda Chem Ind Ltd 脂溶性ビタミン類の分析法および試薬
JPH06341980A (ja) * 1993-02-24 1994-12-13 Kureha Chem Ind Co Ltd ビタミンｄ３誘導体の定量法
JPH0735736A (ja) * 1993-07-19 1995-02-07 Snow Brand Milk Prod Co Ltd 液体クロマトグラフィー用分離剤並びにそれを使用する分離及び分析方法
JPH08248022A (ja) * 1995-03-14 1996-09-27 Kanebo Ltd 皮膚コレステロール過酸化物の定量法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102253130A (zh) * 2010-12-24 2011-11-23 天津天狮生物发展有限公司 测定复合维生素中维生素a、维生素d和维生素e的方法

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Publication number Publication date
ES2133133A1 (es) 1999-08-16

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