ES2133133A1 - Metodo e instalacion para la detrminacion cuantitativa de hidroximetabolitos de la vitamina d3 de forma continua y automatica. - Google Patents

Metodo e instalacion para la detrminacion cuantitativa de hidroximetabolitos de la vitamina d3 de forma continua y automatica.

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Publication number
ES2133133A1
ES2133133A1 ES09702577A ES9702577A ES2133133A1 ES 2133133 A1 ES2133133 A1 ES 2133133A1 ES 09702577 A ES09702577 A ES 09702577A ES 9702577 A ES9702577 A ES 9702577A ES 2133133 A1 ES2133133 A1 ES 2133133A1
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Spain
Prior art keywords
vitamin
liquid
hydroxymetabolites
continuously
automatically
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ES09702577A
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English (en)
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ES2133133B1 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ORTIZ BOYER FRANCISCA
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ORTIZ BOYER FRANCISCA
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Priority to ES9702577A priority Critical patent/ES2133133B1/es
Publication of ES2133133A1 publication Critical patent/ES2133133A1/es
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Abstract

Método e instalación para la determinación cuantitativa de hidroximetabolitos de la vitamina D{sub,3 de forma continua y automática. El método comprende: (a) extracción líquido-líquido manual de la muestra; (b) limpieza y preconcentración del extracto por extracción líquido-sólido; (c) separación-detección del extracto por cromatografía de líquidos con detección UV-visible, estando sincronizadas las etapas (b) y (c) por una unidad de control. La instalación comprende esencialmente una bomba peristáltica (BP), un muestreador automático (TM), una válvula de selección (SV1), una válvula de inyección (IV1) y una minicolumna de extracción (MC). Aplicación en el sector técnico de los métodos analíticos para la determinación de vitaminas y sus metabolitos, concretamente vitamina D{sub,3 .
ES9702577A 1997-12-11 1997-12-11 Metodo e instalacion para la determinacion cuantitativa de hidroximetabolitos de la vitamina d3 de forma continua y automatica. Expired - Fee Related ES2133133B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9702577A ES2133133B1 (es) 1997-12-11 1997-12-11 Metodo e instalacion para la determinacion cuantitativa de hidroximetabolitos de la vitamina d3 de forma continua y automatica.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9702577A ES2133133B1 (es) 1997-12-11 1997-12-11 Metodo e instalacion para la determinacion cuantitativa de hidroximetabolitos de la vitamina d3 de forma continua y automatica.

Publications (2)

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ES2133133A1 true ES2133133A1 (es) 1999-08-16
ES2133133B1 ES2133133B1 (es) 2000-05-01

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ES9702577A Expired - Fee Related ES2133133B1 (es) 1997-12-11 1997-12-11 Metodo e instalacion para la determinacion cuantitativa de hidroximetabolitos de la vitamina d3 de forma continua y automatica.

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ES (1) ES2133133B1 (es)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2334742A1 (es) * 2008-06-17 2010-03-15 Sanidad Y Residencias 21, S.A. Procedimiento automatizado para la determinacion simultanea de vitaminas liposolubles.
CN102253130A (zh) * 2010-12-24 2011-11-23 天津天狮生物发展有限公司 测定复合维生素中维生素a、维生素d和维生素e的方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61160055A (ja) * 1985-01-09 1986-07-19 Masaki Hasegawa 分離剤
SU1363060A1 (ru) * 1986-07-04 1987-12-30 Научно-исследовательский институт животноводства Лесостепи и Полесья УССР Способ количественного определени витамина Д @ в растительных кормах
JPS63158455A (ja) * 1986-12-22 1988-07-01 Takeda Chem Ind Ltd 脂溶性ビタミン類の分析法および試薬
JPH06341980A (ja) * 1993-02-24 1994-12-13 Kureha Chem Ind Co Ltd ビタミンｄ３誘導体の定量法
EP0635300A1 (en) * 1993-07-19 1995-01-25 Snow Brand Milk Products Co., Ltd. Column packings for liquid chromatography
JPH08248022A (ja) * 1995-03-14 1996-09-27 Kanebo Ltd 皮膚コレステロール過酸化物の定量法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61160055A (ja) * 1985-01-09 1986-07-19 Masaki Hasegawa 分離剤
SU1363060A1 (ru) * 1986-07-04 1987-12-30 Научно-исследовательский институт животноводства Лесостепи и Полесья УССР Способ количественного определени витамина Д @ в растительных кормах
JPS63158455A (ja) * 1986-12-22 1988-07-01 Takeda Chem Ind Ltd 脂溶性ビタミン類の分析法および試薬
JPH06341980A (ja) * 1993-02-24 1994-12-13 Kureha Chem Ind Co Ltd ビタミンｄ３誘導体の定量法
EP0635300A1 (en) * 1993-07-19 1995-01-25 Snow Brand Milk Products Co., Ltd. Column packings for liquid chromatography
JPH08248022A (ja) * 1995-03-14 1996-09-27 Kanebo Ltd 皮膚コレステロール過酸化物の定量法

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BASE DE DATOS INSPEC en STN, Institute of Electrical Engineers (Stevenage, GB) AN 82:1926585, FOHLMAN, J. et al. "Vitamin D metabolites from human plasma and mass spectrometric analysis", resumen, Nuclear Instruments and Methods in Physics Research (1 de julio de 1982), Vol. 198, no 1, paginas 169-173. *
BASE DE DATOS PAJ en EPOQUE. Japanese Patent Information Organisation (Tokio, JP) & JP 61160055 A (HASEGAWA, M) 19.07.1986 *
BASE DE DATOS WPI en EPOQUE. Derwent Publications Ltd., (Londres) AN 88-204154 29! & SU 1363060 A1 (UKR LIVESTOCK RES) 30.12.1987, *
PATENT ABSTRACTS OF JAPAN. Japanese Patent Information Organisation (Tokio, JP) Disco 1997 - 1[024], & JP 8248022 A (KANEBO LTD) 27.09.1996 *
PATENT ABSTRACTS OF JAPAN. Japanese Patent Information Organisation (Tokio, JP) Disco PAJ | G01MNP 1988-1993 (2/2) | 51 & JP 63158455 A (TAKEDA CHEM IND LTD) 01.07.1988 *
PATENT ABSTRACTS OF JAPAN. Japanese Patent Information Organisation (Tokio, JP), Disco 1995 - 3[003], & JP 6341980 A (KUREHA CHEM IND CO LTD) 13.12.1994 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2334742A1 (es) * 2008-06-17 2010-03-15 Sanidad Y Residencias 21, S.A. Procedimiento automatizado para la determinacion simultanea de vitaminas liposolubles.
CN102253130A (zh) * 2010-12-24 2011-11-23 天津天狮生物发展有限公司 测定复合维生素中维生素a、维生素d和维生素e的方法

Also Published As

Publication number Publication date
ES2133133B1 (es) 2000-05-01

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