ES2120427T3 - Metodo de radioinmunoensayo para la deteccion de pthrp. - Google Patents

Metodo de radioinmunoensayo para la deteccion de pthrp.

Info

Publication number
ES2120427T3
ES2120427T3 ES92118608T ES92118608T ES2120427T3 ES 2120427 T3 ES2120427 T3 ES 2120427T3 ES 92118608 T ES92118608 T ES 92118608T ES 92118608 T ES92118608 T ES 92118608T ES 2120427 T3 ES2120427 T3 ES 2120427T3
Authority
ES
Spain
Prior art keywords
antibody
antigen
radio
pthrp
immuno
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92118608T
Other languages
English (en)
Inventor
Kinya Miyoshi
Masato Okada
Makoto Takada
Mayumi Goto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Chemical Corp
Original Assignee
Mitsubishi Chemical Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Chemical Corp filed Critical Mitsubishi Chemical Corp
Application granted granted Critical
Publication of ES2120427T3 publication Critical patent/ES2120427T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds
    • Y10S436/817—Steroids or hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Endocrinology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

UN RADIOINMUNOENSAYO QUE COMPRENDE LOS PASOS DE REACCIONAR UN PRIMER ANTICUERPO (A) EN UN SOLIDO INSOLUBLE, QUE ES ESPECIFICO PARA UN ANTIGENO B(B); UN ANTIGENO (B); Y UN SEGUNDO ANTICUERPO (C) DERIVADO DE UN ANIMAL DIFERENTE DEL ANIMAL DEL PRIMER ANTICUERPO (A) QUE SE ENLAZA AL ANTIGENO (B) BAJO CONDICIONES EN DONDE LA CONCENTRACION DE SEGUNDO ANTICUERPO (C) ES MAYOR QUE LA CONSTANTE DE DISOCIACION ENTRE EL ANTICUERPO SEGUNDO (C) Y EL ANTIGENO (B) PARA OBTENER UN COMPLEJO INMUNE (D); REACCIONAR EL COMPLEJO INMUNE (D) CON UNA SONDA MARCADA CON RADIO (E) Y ENTONCES MEDIR LA RADIOACTIVIDAD DEL PRECIPITADO SOLIDO O LA MEZCLA DE REACCION, PARA MEDIR LA CANTIDAD DE ANTIGENO (B). EL METODO OFRECE UN ENSAYO CON UNA ALTA SENSIBILIDAD QUE NO DEPENDE DE LA SENSIBILIDAD DEL SEGUNDO ANTICUERPO UTILIZADO.
ES92118608T 1991-10-31 1992-10-30 Metodo de radioinmunoensayo para la deteccion de pthrp. Expired - Lifetime ES2120427T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP31150691 1991-10-31

Publications (1)

Publication Number Publication Date
ES2120427T3 true ES2120427T3 (es) 1998-11-01

Family

ID=18018058

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92118608T Expired - Lifetime ES2120427T3 (es) 1991-10-31 1992-10-30 Metodo de radioinmunoensayo para la deteccion de pthrp.

Country Status (4)

Country Link
US (1) US5366859A (es)
EP (1) EP0540021B1 (es)
DE (1) DE69226148T2 (es)
ES (1) ES2120427T3 (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL117175A (en) * 1995-02-20 2005-11-20 Sankyo Co Osteoclastogenesis inhibitory factor protein
US5811242A (en) * 1995-10-24 1998-09-22 Tokuyama Corporation Marker and reagent for diabetes mellitus and diabetes mellitus complication
US6087088A (en) * 1997-01-31 2000-07-11 Bayer Corporation Binding assays using more than one label for determining analyte in the presence of interfering factors
NZ335759A (en) 1997-09-24 2002-02-01 Sankyo Co Method of diagnosing metabolic bone diseases by measuring osteoclastgenesis inhibitory factor (OCIF)
US6153440A (en) * 1998-09-23 2000-11-28 The Regents Of The University Of California Simultaneous measurement of free triiodothyronine and free thyroxine by equilibrium dialysis and immunoassay
AT407674B (de) * 1998-09-29 2001-05-25 Biomedica Gmbh Verfahren zur bestimmung von atrialem natriuretischem peptid (anp)
CA2466443A1 (en) * 2001-11-09 2003-05-15 Neopharm, Inc. Selective treatment of il-13 expressing tumors
KR100530611B1 (ko) * 2002-11-22 2005-11-22 한국원자력연구소 대식세포 유주저지인자의 농도 측정을 위한 방사면역측정법
WO2004087758A2 (en) * 2003-03-26 2004-10-14 Neopharm, Inc. Il 13 receptor alpha 2 antibody and methods of use
ES2550004T3 (es) 2006-04-04 2015-11-03 Singulex, Inc. Sistema de alta sensibilidad y métodos de análisis de la troponina
US7838250B1 (en) 2006-04-04 2010-11-23 Singulex, Inc. Highly sensitive system and methods for analysis of troponin
US8450069B2 (en) 2009-06-08 2013-05-28 Singulex, Inc. Highly sensitive biomarker panels
JP4686639B1 (ja) * 2010-02-25 2011-05-25 田中貴金属工業株式会社 生豚肉検出法およびその検出キット
US10648970B2 (en) * 2011-11-08 2020-05-12 Agilent Technologies, Inc. Method for evaluation of target in histological sample
EP3107569A4 (en) 2014-02-20 2018-02-21 Alder Biopharmaceuticals, Inc. Anti-acth antibodies and use thereof
WO2023004248A1 (en) * 2021-07-23 2023-01-26 The Regents Of The University Of Michigan Deprotection-counting probes for detecting and quantifying single molecular analytes

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4034074A (en) * 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US4496654A (en) * 1983-04-08 1985-01-29 Quidel Detection of HCG with solid phase support having avidin coating
US4960716A (en) * 1984-05-01 1990-10-02 Ciba Corning Diagnostics Corp. Monoclonal antibodies specific for 330 KD breast tumor antigen and assay using said monoclonal antibodies
US4748110A (en) * 1985-09-25 1988-05-31 Abbott Laboratories Immunoassay for HTLV-III antigens
JPH081438B2 (ja) * 1986-05-13 1996-01-10 三洋化成工業株式会社 酵素免疫測定法
JP2681370B2 (ja) * 1988-06-30 1997-11-26 塩野義製薬株式会社 免疫測定法

Also Published As

Publication number Publication date
DE69226148T2 (de) 1998-12-17
US5366859A (en) 1994-11-22
EP0540021B1 (en) 1998-07-08
EP0540021A1 (en) 1993-05-05
DE69226148D1 (de) 1998-08-13

Similar Documents

Publication Publication Date Title
ES2120427T3 (es) Metodo de radioinmunoensayo para la deteccion de pthrp.
US3720760A (en) Method for determining the presence of reagin-immunoglobulins(reagin-ig)directed against certain allergens,in aqueous samples
Voller et al. Enzyme immunoassays in diagnostic medicine: theory and practice
Hjiyiannakis et al. Thyroglobulin antibodies in differentiated thyroid cancer
SE7709232L (sv) Forfarande och anordning for bestemning av antigener och antikroppar
MX174477B (es) Inmunoensayo amperometrico homogeneo
JPS6488155A (en) Immunological inspection for detecting antibody against antigen
ATE77488T1 (de) In-vitro-testverfahren zum nachweis zellulaerer immunresponsen.
GB1528071A (en) Radioimmunoassay
SE7614183L (sv) Forfarande och anordning ffor graviditetstest
DK509686A (da) Fremgangsmaade og apparat til bestemmelse af antistofindholdet i blod
SE7600232L (sv) Immunologiskt reagens och sett att anvenda detsamma
ES2186121T3 (es) Mejora de los resultados de los dosificados de inmunodeteccion gracias al empleo de varios trazadores.
DK516387A (da) Fremgangsmaade til immunometrisk bestemmelse
Detrick-Hooks et al. Quantitative comparison of techniques used to measure complement-mediated cytotoxicity of nucleated cells
EP0303229A3 (en) Method of high sensitivity immunoassay
KR870002456A (ko) Tsh의 측정방법
ATE220098T1 (de) Eine petroleum immuntestmethode und ihre komponenten
Abreau et al. Clinical evaluation of a hemagglutination method for microsomal and thyroglobulin antibodies in autoimmune thyroid disease
Lippi et al. Not all SARS-CoV-2 IgG and neutralizing antibody assays are created equal
Centifanto et al. A simplified method for measuring human IgE
Wolf et al. Antibodies to sperm-associated antigens detected by solid phase assays
EP0246615A3 (en) Method and a kit for diagnosing plant diseases
KR850008620A (ko) Atl 바이러스 항체의 측정방법 및 시약
Singer et al. Quality control of the latex-fixation test

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 540021

Country of ref document: ES