ES2114951T3 - Procedimiento para el rastreo de sustancias con efecto modulador sobre una via de transmision de señales celular, dependiente del receptor. - Google Patents

Procedimiento para el rastreo de sustancias con efecto modulador sobre una via de transmision de señales celular, dependiente del receptor.

Info

Publication number
ES2114951T3
ES2114951T3 ES92923795T ES92923795T ES2114951T3 ES 2114951 T3 ES2114951 T3 ES 2114951T3 ES 92923795 T ES92923795 T ES 92923795T ES 92923795 T ES92923795 T ES 92923795T ES 2114951 T3 ES2114951 T3 ES 2114951T3
Authority
ES
Spain
Prior art keywords
substances
receiver
procedure
signal transmission
transmission way
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92923795T
Other languages
English (en)
Inventor
Armin Peter Czernilofsky
Adolf Himmler
Christian Stratowa
Ulrike Weyer
Herbert Lamche
Renate Schaefer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim International GmbH
Original Assignee
Boehringer Ingelheim International GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim International GmbH filed Critical Boehringer Ingelheim International GmbH
Application granted granted Critical
Publication of ES2114951T3 publication Critical patent/ES2114951T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE DESCRIBE UN PROCEDIMIENTO PARA EL APANTALLADO DE SUSTANCIAS QUE DISPONEN DE UN EFECTO DE MODULACION SOBRE UNA VIA DE TRANSMISION DE SEÑAL DEPENDIENTE DE REACTOR EN CELULAS DE MAMIFEROS. LAS CELULAS SON UTILIZADAS TRANSFORMADAS CON UN GENE DE INFORMACION Y CON UNA SECUENCIA REGULADORA FUNCIONALMENTE LIGADA DE FORMA SENSITIVA A LA CONCENTRACION IP3/DSG, ASI COMO CON UNA CODIFICACION DNA PARA UN RECEPTOR ACOPLADO AL SISTEMA DE EFECTO FOSFOLIPASA, EN PARTICULAR RECEPTOR ACOPLADO A LA PROTEINA G. LA UTILIZACION DE CELULAS DE REFERENCIA SIN RECEPTOR ADN Y CELULAS DE REFERENCIA CON CARACTER ESPECIFICO PARA EL SISTEMA DE EFECTO ADENILATO CILASA PERMITE A LAS SUSTANCIAS A SER IDENTIFICADAS DISPONIENDO DE POTENCIAL DE ACCION FARMACOLOGICA Y CONDICIONES ESPECIFICAS PARA UNA DETERMINADA VIA DE TRANSMISION DE SEÑAL DEPENDIENDO DEL RECEPTOR.
ES92923795T 1991-11-25 1992-11-25 Procedimiento para el rastreo de sustancias con efecto modulador sobre una via de transmision de señales celular, dependiente del receptor. Expired - Lifetime ES2114951T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4138621A DE4138621A1 (de) 1991-11-25 1991-11-25 Verfahren zum screenen von substanzen mit modulierender wirkung auf einen rezeptorabhaengigen zellulaeren signaluebertragungsweg

Publications (1)

Publication Number Publication Date
ES2114951T3 true ES2114951T3 (es) 1998-06-16

Family

ID=6445474

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92923795T Expired - Lifetime ES2114951T3 (es) 1991-11-25 1992-11-25 Procedimiento para el rastreo de sustancias con efecto modulador sobre una via de transmision de señales celular, dependiente del receptor.

Country Status (10)

Country Link
US (1) US5854004A (es)
EP (1) EP0621901B1 (es)
JP (1) JP3571718B2 (es)
AT (1) ATE165115T1 (es)
AU (1) AU668118B2 (es)
CA (1) CA2122356A1 (es)
DE (2) DE4138621A1 (es)
ES (1) ES2114951T3 (es)
TW (1) TW380162B (es)
WO (1) WO1993011257A2 (es)

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US6921751B1 (en) 1998-05-20 2005-07-26 Erasmus Universiteit Rotterdam Immunoregulator
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CA2342926C (en) * 1998-09-04 2006-10-31 Aventis Pharmaceuticals Products Inc. Luciferase reporter bioassay of parathyroid hormone compounds
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AU1447600A (en) 1998-10-22 2000-05-08 Thomas J. Gardella Bioactive peptides and peptide derivatives of parathyroid hormone (pth) and parathyroid hormone-related peptide (pthrp)
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US20030219825A1 (en) * 2000-06-13 2003-11-27 Haizlip Jill Elaine High throughput method for screening candidate compounds for biological activity
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CA2421858A1 (en) * 2000-09-07 2002-03-14 Bjorn A. Olde Reporter system for cell surface receptor-ligand binding
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Also Published As

Publication number Publication date
JPH07505046A (ja) 1995-06-08
JP3571718B2 (ja) 2004-09-29
AU668118B2 (en) 1996-04-26
ATE165115T1 (de) 1998-05-15
EP0621901A1 (de) 1994-11-02
DE4138621A1 (de) 1993-06-17
DE59209288D1 (de) 1998-05-20
CA2122356A1 (en) 1993-06-10
WO1993011257A3 (de) 1993-06-24
US5854004A (en) 1998-12-29
AU2945592A (en) 1993-06-28
TW380162B (en) 2000-01-21
EP0621901B1 (de) 1998-04-15
WO1993011257A2 (de) 1993-06-10

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