ES2091777T3 - Procedimiento para preparar un ensayo inmunologico de transferencia western. - Google Patents
Procedimiento para preparar un ensayo inmunologico de transferencia western.Info
- Publication number
- ES2091777T3 ES2091777T3 ES90108710T ES90108710T ES2091777T3 ES 2091777 T3 ES2091777 T3 ES 2091777T3 ES 90108710 T ES90108710 T ES 90108710T ES 90108710 T ES90108710 T ES 90108710T ES 2091777 T3 ES2091777 T3 ES 2091777T3
- Authority
- ES
- Spain
- Prior art keywords
- preparing
- procedure
- immunological test
- western transfer
- hiv
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 3
- 230000001900 immune effect Effects 0.000 title 1
- 239000000427 antigen Substances 0.000 abstract 2
- 102000036639 antigens Human genes 0.000 abstract 2
- 108091007433 antigens Proteins 0.000 abstract 2
- 241000725303 Human immunodeficiency virus Species 0.000 abstract 1
- 241000713772 Human immunodeficiency virus 1 Species 0.000 abstract 1
- 230000000890 antigenic effect Effects 0.000 abstract 1
- 238000001962 electrophoresis Methods 0.000 abstract 1
- 238000003018 immunoassay Methods 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 238000005215 recombination Methods 0.000 abstract 1
- 230000006798 recombination Effects 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
- G01N33/561—Immunoelectrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
- G01N33/56988—HIV or HTLV
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/974—Aids related test
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S930/00—Peptide or protein sequence
- Y10S930/01—Peptide or protein sequence
- Y10S930/22—Viral peptide or viral protein
- Y10S930/221—Retrovirus related, or human immunodeficiency virus related, or simian immunodeficiency virus related
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- AIDS & HIV (AREA)
- Tropical Medicine & Parasitology (AREA)
- Electrochemistry (AREA)
- Peptides Or Proteins (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Steroid Compounds (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
LA PRESENTE INVENCION COMPRENDE UN METODO PERFECCIONADO DE INMUNOENSAYO, QUE IMPLICA LA TRANSFERENCIA SIMULTANEA DE ANTIGENOS MULTIPLES SOBRE UN SOPORTE SOLIDO UNICO. LOS ANTIGENOS QUE PUEDEN UTILIZARSE MEDIANTE ESTE METODO PUEDEN OBTENERSE POR MEDIOS NATURALES O MEDIANTE RECOMBINACIONES, Y SE PUEDEN PURIFICAR SOBRE UNA VARIEDAD DE GEL DE ELECTROFORESIS. ESTE METODO RESULTA ESPECIALMENTE UTIL PARA PROPORCIONAR UN ENSAYO DE COMPONENTES MULTIPLES, EXTREMADAMENTE SENSIBLE,, UTILIZANDO POLIPEPTIDOS ANTIGENICOS ESENCIALMENTE PUROS, CAPACES DE DETECTAR LA PRESENCIA DE ANTICUERPOS A LOS VIRUS HIV-1 O HIV-2 (DEL SIDA) EN PACIENTES INFECTADOS.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US35018089A | 1989-05-09 | 1989-05-09 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2091777T3 true ES2091777T3 (es) | 1996-11-16 |
Family
ID=23375548
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES90108710T Expired - Lifetime ES2091777T3 (es) | 1989-05-09 | 1990-05-09 | Procedimiento para preparar un ensayo inmunologico de transferencia western. |
Country Status (9)
Country | Link |
---|---|
US (1) | US5356772A (es) |
EP (1) | EP0397129B1 (es) |
JP (1) | JP2945074B2 (es) |
KR (1) | KR900018680A (es) |
AT (1) | ATE140540T1 (es) |
AU (1) | AU637097B2 (es) |
CA (1) | CA2016313C (es) |
DE (1) | DE69027812T2 (es) |
ES (1) | ES2091777T3 (es) |
Families Citing this family (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5120662A (en) * | 1989-05-09 | 1992-06-09 | Abbott Laboratories | Multilayer solid phase immunoassay support and method of use |
EP0473065A3 (en) * | 1990-08-29 | 1992-08-26 | Abbott Laboratories | Simultaneous assay for detecting two or more analytes |
CA2162568C (en) * | 1993-05-10 | 2001-10-30 | Yuichi Oku | Method and reagent for simultaneously assaying one or more ligands in a group of preselected ligands |
EP0627625A1 (en) * | 1993-06-04 | 1994-12-07 | Abbott Laboratories | Immunoassay for differential diagnosis |
US5731161A (en) * | 1995-04-24 | 1998-03-24 | Allergan, Inc. | Botulinum toxin antibody detection assay |
US6020122A (en) * | 1995-06-07 | 2000-02-01 | Abbott Laboratories | Hepatitis C virus second envelope (HCV-E2) glycoprotein expression system |
IT1290804B1 (it) | 1996-08-16 | 1998-12-11 | Abbott Lab | Saggio western blot perfezionato per l'individuazione di anticorpi specifici contro il virus hcmv |
US6680208B1 (en) | 1999-11-19 | 2004-01-20 | Becton, Dickinson And Company | Rapid protein identification using antibody mixtures |
US6933109B2 (en) * | 2000-12-22 | 2005-08-23 | Large Scale Proteomics Corporation | Rapid particle detection |
WO2006091525A2 (en) * | 2005-02-24 | 2006-08-31 | Invitrogen Corporation | Electro-blotting devices, systems, and kits, and methods for their use |
US7749717B2 (en) * | 2006-10-19 | 2010-07-06 | Abbott Laboratories | Methods for the detection and diagnosis of Trypanosoma cruzi infection |
US8075755B2 (en) * | 2007-12-13 | 2011-12-13 | Bio-Rad Laboratories, Inc. | Polymeric sorbent sheets as ion reservoirs for electroblotting |
WO2009126764A1 (en) | 2008-04-11 | 2009-10-15 | Cytotech Labs, Llc | Methods and use of inducing apoptosis in cancer cells |
EP2310857A4 (en) | 2008-07-11 | 2011-11-30 | Life Technologies Corp | ELECTROPHORETICALLY REINFORCED DETECTION OF ANALYTES ON A SOLID CARRIER |
US8715476B2 (en) | 2009-12-11 | 2014-05-06 | Bio-Rad Laboratories, Inc. | Instrument for independent electrotransfer in multiple cassettes |
ES2625406T3 (es) | 2010-03-25 | 2017-07-19 | Oregon Health & Science University | Glicoproteínas de CMV y vectores recombinantes |
SI2691530T1 (en) | 2011-06-10 | 2018-08-31 | Oregon Health & Science University | CMV glycoproteins and recombinant vectors |
US20130189754A1 (en) | 2011-09-12 | 2013-07-25 | International Aids Vaccine Initiative | Immunoselection of recombinant vesicular stomatitis virus expressing hiv-1 proteins by broadly neutralizing antibodies |
US9402894B2 (en) | 2011-10-27 | 2016-08-02 | International Aids Vaccine Initiative | Viral particles derived from an enveloped virus |
EP2679596B1 (en) | 2012-06-27 | 2017-04-12 | International Aids Vaccine Initiative | HIV-1 env glycoprotein variant |
US9594054B2 (en) | 2012-07-25 | 2017-03-14 | Bio-Rad Laboratories, Inc. | Targeted delivery of reagents to spots on a planar support through patterned transfer sheets |
CN104937401B (zh) | 2012-10-17 | 2018-03-09 | 生物辐射实验室股份有限公司 | 用于大分析物阵列的图像捕获 |
US9291596B2 (en) | 2013-02-12 | 2016-03-22 | Pierce Biotechnology, Inc. | Electroblot transfer buffer |
USD738527S1 (en) | 2013-05-28 | 2015-09-08 | Life Technologies Corporation | Electroblotting apparatus |
EP2848937A1 (en) | 2013-09-05 | 2015-03-18 | International Aids Vaccine Initiative | Methods of identifying novel HIV-1 immunogens |
US10058604B2 (en) | 2013-10-07 | 2018-08-28 | International Aids Vaccine Initiative | Soluble HIV-1 envelope glycoprotein trimers |
US10174292B2 (en) | 2015-03-20 | 2019-01-08 | International Aids Vaccine Initiative | Soluble HIV-1 envelope glycoprotein trimers |
US9931394B2 (en) | 2015-03-23 | 2018-04-03 | International Aids Vaccine Initiative | Soluble HIV-1 envelope glycoprotein trimers |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4452901A (en) * | 1980-03-20 | 1984-06-05 | Ciba-Geigy Corporation | Electrophoretically transferring electropherograms to nitrocellulose sheets for immuno-assays |
AR231590A1 (es) * | 1981-04-29 | 1984-12-28 | Ciba Geigy Ag | Dispositivo de analisis inmunologico y procedimiento para obtenerlo |
NZ228756A (en) * | 1984-04-23 | 1990-10-26 | Us Health | Detection of hiv antigen using competition immune assay or western blot assay |
ZA855031B (en) * | 1984-08-31 | 1986-02-26 | New York Blood Center Inc | Assay for simultaneous detection of antigen and antibody in given serum |
US4843011A (en) * | 1985-08-01 | 1989-06-27 | Akzo N.V. | Monoclonal antibodies for binding HTLV-III proteins, and cell lines for their production |
CH669265A5 (de) * | 1985-12-18 | 1989-02-28 | Celldynamics Ag | Multiindikator-teststreifen fuer immunologische analysen, ihre herstellung und verwendung. |
US4980279A (en) * | 1986-04-15 | 1990-12-25 | Scripps Clinic And Research Foundation | Cellular fibronectin: polypeptides, anti-polypeptide antibodies and assay methods |
US4816387A (en) * | 1986-06-06 | 1989-03-28 | Bio-Research Laboratories, Inc. | Method for detection of antibodies to HTLV-III and diagnostic test kit useful therewith |
ATE130050T1 (de) * | 1987-07-13 | 1995-11-15 | Verigen Inc | Verfahren zum schnellen und empfindlichen nachweis von hiv-1-antikörpern. |
JPH01113663A (ja) * | 1987-10-28 | 1989-05-02 | Eisai Co Ltd | Atlv抗体の測定試薬および測定方法 |
-
1990
- 1990-05-03 AU AU54742/90A patent/AU637097B2/en not_active Ceased
- 1990-05-08 CA CA002016313A patent/CA2016313C/en not_active Expired - Fee Related
- 1990-05-09 KR KR1019900006519A patent/KR900018680A/ko not_active Application Discontinuation
- 1990-05-09 AT AT90108710T patent/ATE140540T1/de not_active IP Right Cessation
- 1990-05-09 ES ES90108710T patent/ES2091777T3/es not_active Expired - Lifetime
- 1990-05-09 EP EP90108710A patent/EP0397129B1/en not_active Expired - Lifetime
- 1990-05-09 DE DE69027812T patent/DE69027812T2/de not_active Expired - Fee Related
- 1990-05-09 JP JP2119648A patent/JP2945074B2/ja not_active Expired - Fee Related
- 1990-12-05 US US07/622,311 patent/US5356772A/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
AU637097B2 (en) | 1993-05-20 |
CA2016313C (en) | 2002-12-31 |
JPH0328765A (ja) | 1991-02-06 |
DE69027812T2 (de) | 1997-02-13 |
EP0397129B1 (en) | 1996-07-17 |
DE69027812D1 (de) | 1996-08-22 |
EP0397129A3 (en) | 1991-11-13 |
EP0397129A2 (en) | 1990-11-14 |
JP2945074B2 (ja) | 1999-09-06 |
CA2016313A1 (en) | 1990-11-09 |
KR900018680A (ko) | 1990-12-22 |
US5356772A (en) | 1994-10-18 |
ATE140540T1 (de) | 1996-08-15 |
AU5474290A (en) | 1990-11-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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