DE69027812T2 - Verfahren zur Zurichtung eines verbesserten Western-Blott-Immuntestverfahrens - Google Patents

Verfahren zur Zurichtung eines verbesserten Western-Blott-Immuntestverfahrens

Info

Publication number
DE69027812T2
DE69027812T2 DE69027812T DE69027812T DE69027812T2 DE 69027812 T2 DE69027812 T2 DE 69027812T2 DE 69027812 T DE69027812 T DE 69027812T DE 69027812 T DE69027812 T DE 69027812T DE 69027812 T2 DE69027812 T2 DE 69027812T2
Authority
DE
Germany
Prior art keywords
procedure
preparing
immunoassay
hiv
western blott
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69027812T
Other languages
English (en)
Other versions
DE69027812D1 (de
Inventor
Emerson W Chan
Werner Schulze
William Gerard Robey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Application granted granted Critical
Publication of DE69027812D1 publication Critical patent/DE69027812D1/de
Publication of DE69027812T2 publication Critical patent/DE69027812T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G01N33/561—Immunoelectrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56988—HIV or HTLV
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/974—Aids related test
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/22—Viral peptide or viral protein
    • Y10S930/221—Retrovirus related, or human immunodeficiency virus related, or simian immunodeficiency virus related

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • AIDS & HIV (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Electrochemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Steroid Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
DE69027812T 1989-05-09 1990-05-09 Verfahren zur Zurichtung eines verbesserten Western-Blott-Immuntestverfahrens Expired - Fee Related DE69027812T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US35018089A 1989-05-09 1989-05-09

Publications (2)

Publication Number Publication Date
DE69027812D1 DE69027812D1 (de) 1996-08-22
DE69027812T2 true DE69027812T2 (de) 1997-02-13

Family

ID=23375548

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69027812T Expired - Fee Related DE69027812T2 (de) 1989-05-09 1990-05-09 Verfahren zur Zurichtung eines verbesserten Western-Blott-Immuntestverfahrens

Country Status (9)

Country Link
US (1) US5356772A (de)
EP (1) EP0397129B1 (de)
JP (1) JP2945074B2 (de)
KR (1) KR900018680A (de)
AT (1) ATE140540T1 (de)
AU (1) AU637097B2 (de)
CA (1) CA2016313C (de)
DE (1) DE69027812T2 (de)
ES (1) ES2091777T3 (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5120662A (en) * 1989-05-09 1992-06-09 Abbott Laboratories Multilayer solid phase immunoassay support and method of use
EP0473065A3 (en) * 1990-08-29 1992-08-26 Abbott Laboratories Simultaneous assay for detecting two or more analytes
US5789165A (en) * 1993-05-10 1998-08-04 Nissui Pharmaceutical Co., Ltd. Method and reagent for simultaneously assaying one or more ligands in a group of preselected ligands
EP0627625A1 (de) * 1993-06-04 1994-12-07 Abbott Laboratories Immunoassay für Differentialdiagnose
US5731161A (en) * 1995-04-24 1998-03-24 Allergan, Inc. Botulinum toxin antibody detection assay
US6020122A (en) * 1995-06-07 2000-02-01 Abbott Laboratories Hepatitis C virus second envelope (HCV-E2) glycoprotein expression system
IT1290804B1 (it) * 1996-08-16 1998-12-11 Abbott Lab Saggio western blot perfezionato per l'individuazione di anticorpi specifici contro il virus hcmv
US6680208B1 (en) 1999-11-19 2004-01-20 Becton, Dickinson And Company Rapid protein identification using antibody mixtures
US6933109B2 (en) * 2000-12-22 2005-08-23 Large Scale Proteomics Corporation Rapid particle detection
JP5214979B2 (ja) 2005-02-24 2013-06-19 ライフ テクノロジーズ コーポレーション エレクトロブロッティング装置、システム及びキット、並びにそれらの使用方法
US7749717B2 (en) * 2006-10-19 2010-07-06 Abbott Laboratories Methods for the detection and diagnosis of Trypanosoma cruzi infection
US8075755B2 (en) * 2007-12-13 2011-12-13 Bio-Rad Laboratories, Inc. Polymeric sorbent sheets as ion reservoirs for electroblotting
WO2009126764A1 (en) 2008-04-11 2009-10-15 Cytotech Labs, Llc Methods and use of inducing apoptosis in cancer cells
US20100044229A1 (en) * 2008-07-11 2010-02-25 Life Technologies Corporation Electrophoretically Enhanced Detection of Analytes on a Solid Support
US8715476B2 (en) 2009-12-11 2014-05-06 Bio-Rad Laboratories, Inc. Instrument for independent electrotransfer in multiple cassettes
CA2793959C (en) 2010-03-25 2019-06-04 Oregon Health & Science University Cmv glycoproteins and recombinant vectors
HUE037408T2 (hu) 2011-06-10 2018-08-28 Univ Oregon Health & Science CMV glikoproteinek és rekombináns vektorok
AU2012216792A1 (en) 2011-09-12 2013-03-28 International Aids Vaccine Initiative Immunoselection of recombinant vesicular stomatitis virus expressing HIV-1 proteins by broadly neutralizing antibodies
EP2586461A1 (de) 2011-10-27 2013-05-01 Christopher L. Parks Von einem eingehüllten Virus abgeleitete Virenpartikel
ES2631608T3 (es) 2012-06-27 2017-09-01 International Aids Vaccine Initiative Variante de la glicoproteína Env del VIH-1
CN104583226B (zh) 2012-07-25 2018-05-04 生物辐射实验室股份有限公司 通过图案化转移片材的试剂靶向递送
EP2909613B1 (de) 2012-10-17 2020-09-02 Bio-rad Laboratories, Inc. Bilderfassung für grosse analytarrays
US9291596B2 (en) 2013-02-12 2016-03-22 Pierce Biotechnology, Inc. Electroblot transfer buffer
USD738527S1 (en) 2013-05-28 2015-09-08 Life Technologies Corporation Electroblotting apparatus
US20150065381A1 (en) 2013-09-05 2015-03-05 International Aids Vaccine Initiative Methods of identifying novel hiv-1 immunogens
US10058604B2 (en) 2013-10-07 2018-08-28 International Aids Vaccine Initiative Soluble HIV-1 envelope glycoprotein trimers
EP3069730A3 (de) 2015-03-20 2017-03-15 International Aids Vaccine Initiative Lösliche hiv-1-hüllglykoproteintrimere
EP3072901A1 (de) 2015-03-23 2016-09-28 International Aids Vaccine Initiative Lösliche hiv-1-hüllglykoproteintrimere

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4452901A (en) * 1980-03-20 1984-06-05 Ciba-Geigy Corporation Electrophoretically transferring electropherograms to nitrocellulose sheets for immuno-assays
AR231590A1 (es) * 1981-04-29 1984-12-28 Ciba Geigy Ag Dispositivo de analisis inmunologico y procedimiento para obtenerlo
CA1247005A (en) * 1984-04-23 1988-12-20 Robert C. Gallo Isolation of protein of htlv-iii, serological detection of antibodies to htlv-iii in sera of patients with aids and pre-aids conditions, and detection of htlv-iii infection by immuno-assays using htlv-iii and its proteins
ZA855031B (en) * 1984-08-31 1986-02-26 New York Blood Center Inc Assay for simultaneous detection of antigen and antibody in given serum
US4843011A (en) * 1985-08-01 1989-06-27 Akzo N.V. Monoclonal antibodies for binding HTLV-III proteins, and cell lines for their production
CH669265A5 (de) * 1985-12-18 1989-02-28 Celldynamics Ag Multiindikator-teststreifen fuer immunologische analysen, ihre herstellung und verwendung.
US4980279A (en) * 1986-04-15 1990-12-25 Scripps Clinic And Research Foundation Cellular fibronectin: polypeptides, anti-polypeptide antibodies and assay methods
US4816387A (en) * 1986-06-06 1989-03-28 Bio-Research Laboratories, Inc. Method for detection of antibodies to HTLV-III and diagnostic test kit useful therewith
ATE130050T1 (de) * 1987-07-13 1995-11-15 Verigen Inc Verfahren zum schnellen und empfindlichen nachweis von hiv-1-antikörpern.
JPH01113663A (ja) * 1987-10-28 1989-05-02 Eisai Co Ltd Ａｔｌｖ抗体の測定試薬および測定方法

Also Published As

Publication number Publication date
CA2016313A1 (en) 1990-11-09
KR900018680A (ko) 1990-12-22
ATE140540T1 (de) 1996-08-15
EP0397129A3 (de) 1991-11-13
EP0397129A2 (de) 1990-11-14
ES2091777T3 (es) 1996-11-16
US5356772A (en) 1994-10-18
JP2945074B2 (ja) 1999-09-06
CA2016313C (en) 2002-12-31
AU637097B2 (en) 1993-05-20
EP0397129B1 (de) 1996-07-17
AU5474290A (en) 1990-11-15
JPH0328765A (ja) 1991-02-06
DE69027812D1 (de) 1996-08-22

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Legal Events

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8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee