ES2083299T3 - Metodos para secuenciacion de oligonucleotidos sinteticos que contienen enlaces internucleotidicos distintos a fosfodiester. - Google Patents
Metodos para secuenciacion de oligonucleotidos sinteticos que contienen enlaces internucleotidicos distintos a fosfodiester.Info
- Publication number
- ES2083299T3 ES2083299T3 ES93923306T ES93923306T ES2083299T3 ES 2083299 T3 ES2083299 T3 ES 2083299T3 ES 93923306 T ES93923306 T ES 93923306T ES 93923306 T ES93923306 T ES 93923306T ES 2083299 T3 ES2083299 T3 ES 2083299T3
- Authority
- ES
- Spain
- Prior art keywords
- synthetic oligonucleotides
- fosfodiester
- methods
- oligonucleotides containing
- sequencing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 238000012163 sequencing technique Methods 0.000 title abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 150000004713 phosphodiesters Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Abstract
LA INVENCION SE REFIERE A OLIGONUCLEOTIDOS SINTETICOS Y EN PARTICULAR A LA DETERMINACION DE SECUENCIAS DE NUCLEOTIDOS DE OLIGONUCLEOTIDOS SINTETICOS QUE TIENEN ENLACES ENTRE NUCLEOTIDOS DISTINTOS DE FOSFODIESTERES. EN LA INVENCION SE PRESENTA UN METODO PARA SECUENCIAR TALES OLIGONUCLEOTIDOS SINTETICOS MODIFICADOS.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/958,133 US5403709A (en) | 1992-10-06 | 1992-10-06 | Method for sequencing synthetic oligonucleotides containing non-phosphodiester internucleotide linkages |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2083299T3 true ES2083299T3 (es) | 1996-04-01 |
Family
ID=25500629
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES93923306T Expired - Lifetime ES2083299T3 (es) | 1992-10-06 | 1993-10-06 | Metodos para secuenciacion de oligonucleotidos sinteticos que contienen enlaces internucleotidicos distintos a fosfodiester. |
Country Status (20)
Country | Link |
---|---|
US (2) | US5403709A (es) |
EP (1) | EP0664836B1 (es) |
JP (1) | JPH08504571A (es) |
KR (1) | KR950704508A (es) |
AT (1) | ATE134220T1 (es) |
AU (1) | AU670843B2 (es) |
BR (1) | BR9307192A (es) |
CA (1) | CA2146365A1 (es) |
CZ (1) | CZ85095A3 (es) |
DE (1) | DE69301570T2 (es) |
DK (1) | DK0664836T3 (es) |
ES (1) | ES2083299T3 (es) |
FI (1) | FI951612A (es) |
GR (1) | GR3019910T3 (es) |
HU (1) | HUT72988A (es) |
NO (1) | NO951328L (es) |
NZ (1) | NZ257032A (es) |
PL (1) | PL308294A1 (es) |
RU (1) | RU95110871A (es) |
WO (1) | WO1994008052A1 (es) |
Families Citing this family (41)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE59209203D1 (de) * | 1991-08-28 | 1998-03-26 | Boehringer Mannheim Gmbh | Primer für matrizenabhängige enzymatische nukleinsäuresynthesen |
US5525470A (en) * | 1994-01-26 | 1996-06-11 | Hybridon, Inc. | Method of sequencing [short] oligonucleotides |
US5589330A (en) * | 1994-07-28 | 1996-12-31 | Genzyme Corporation | High-throughput screening method for sequence or genetic alterations in nucleic acids using elution and sequencing of complementary oligonucleotides |
US6608035B1 (en) | 1994-10-25 | 2003-08-19 | Hybridon, Inc. | Method of down-regulating gene expression |
US6645943B1 (en) * | 1994-10-25 | 2003-11-11 | Hybridon, Inc. | Method of down-regulating gene expression |
EP0718409A3 (en) * | 1994-12-22 | 1999-02-03 | Hitachi, Ltd. | DNA preparation method |
EP0885236A1 (en) * | 1996-01-26 | 1998-12-23 | Codon Pharmaceuticals, Inc. | Oligonucleotide analogs |
US5851804A (en) * | 1996-05-06 | 1998-12-22 | Apollon, Inc. | Chimeric kanamycin resistance gene |
US6291164B1 (en) | 1996-11-22 | 2001-09-18 | Invitrogen Corporation | Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate |
US20040072210A1 (en) * | 1997-07-07 | 2004-04-15 | Billing-Medel Patricia A. | Reagents and methods useful for detecting diseases of the breast |
US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US6780591B2 (en) * | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US6218118B1 (en) | 1998-07-09 | 2001-04-17 | Agilent Technologies, Inc. | Method and mixture reagents for analyzing the nucleotide sequence of nucleic acids by mass spectrometry |
WO2000046402A1 (en) * | 1999-02-05 | 2000-08-10 | Amersham Pharmacia Biotech Uk Limited | Genomic analysis method |
US6818395B1 (en) * | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
US7501245B2 (en) * | 1999-06-28 | 2009-03-10 | Helicos Biosciences Corp. | Methods and apparatuses for analyzing polynucleotide sequences |
EP1072679A3 (en) * | 1999-07-20 | 2002-07-31 | Agilent Technologies, Inc. (a Delaware corporation) | Method of producing nucleic acid molecules with reduced secondary structure |
AU2001236491A1 (en) * | 2000-01-18 | 2003-09-16 | Quantom Dot Corporation | Oligonucleotide-tagged semiconductor nanocrystals for microarray and fluorescence in situ hybridization |
CA2440754A1 (en) | 2001-03-12 | 2002-09-19 | Stephen Quake | Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension |
US20040219532A1 (en) * | 2003-04-30 | 2004-11-04 | Sampson Jeffrey R. | Internal references measurements |
WO2005001129A2 (en) * | 2003-06-06 | 2005-01-06 | Applera Corporation | Mobility cassettes |
US20050170367A1 (en) * | 2003-06-10 | 2005-08-04 | Quake Stephen R. | Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids |
US7169560B2 (en) * | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
US7981604B2 (en) | 2004-02-19 | 2011-07-19 | California Institute Of Technology | Methods and kits for analyzing polynucleotide sequences |
US20060046258A1 (en) * | 2004-02-27 | 2006-03-02 | Lapidus Stanley N | Applications of single molecule sequencing |
US20050239085A1 (en) * | 2004-04-23 | 2005-10-27 | Buzby Philip R | Methods for nucleic acid sequence determination |
US20050260609A1 (en) * | 2004-05-24 | 2005-11-24 | Lapidus Stanley N | Methods and devices for sequencing nucleic acids |
US20070117103A1 (en) * | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
US20070117104A1 (en) * | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
JP2008512084A (ja) * | 2004-05-25 | 2008-04-24 | ヘリコス バイオサイエンシーズ コーポレイション | 核酸の配列決定のための方法およびデバイス |
US7476734B2 (en) * | 2005-12-06 | 2009-01-13 | Helicos Biosciences Corporation | Nucleotide analogs |
US20060024678A1 (en) * | 2004-07-28 | 2006-02-02 | Helicos Biosciences Corporation | Use of single-stranded nucleic acid binding proteins in sequencing |
US20060118754A1 (en) * | 2004-12-08 | 2006-06-08 | Lapen Daniel C | Stabilizing a polyelectrolyte multilayer |
US7220549B2 (en) | 2004-12-30 | 2007-05-22 | Helicos Biosciences Corporation | Stabilizing a nucleic acid for nucleic acid sequencing |
US20060172328A1 (en) * | 2005-01-05 | 2006-08-03 | Buzby Philip R | Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction |
US7482120B2 (en) * | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
US20070117102A1 (en) * | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
WO2007070642A2 (en) * | 2005-12-15 | 2007-06-21 | Helicos Biosciences Corporation | Methods for increasing accuracy of nucleic acid sequencing |
US7397546B2 (en) * | 2006-03-08 | 2008-07-08 | Helicos Biosciences Corporation | Systems and methods for reducing detected intensity non-uniformity in a laser beam |
US20080309926A1 (en) * | 2006-03-08 | 2008-12-18 | Aaron Weber | Systems and methods for reducing detected intensity non uniformity in a laser beam |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4521509A (en) * | 1982-11-24 | 1985-06-04 | Research Corporation | Method for degrading DNA |
US4797355A (en) * | 1985-06-13 | 1989-01-10 | Amgen Inc. | Methods for attaching polynucleotides to supports |
EP0224126A3 (en) * | 1985-11-25 | 1989-02-01 | The University of Calgary | Covalently linked complementary oligodeoxynucleotides as universal nucleic acid sequencing primer linkers |
PT92506A (pt) * | 1988-12-07 | 1990-06-29 | Gen Hospital Corp | Metodo para o enriquecimento e clonagem de dna contendo uma insercao ou correspondendo a uma delecao |
-
1992
- 1992-10-06 US US07/958,133 patent/US5403709A/en not_active Expired - Fee Related
-
1993
- 1993-10-06 HU HU9500993A patent/HUT72988A/hu unknown
- 1993-10-06 AT AT93923306T patent/ATE134220T1/de not_active IP Right Cessation
- 1993-10-06 DE DE69301570T patent/DE69301570T2/de not_active Expired - Fee Related
- 1993-10-06 JP JP6509423A patent/JPH08504571A/ja active Pending
- 1993-10-06 NZ NZ257032A patent/NZ257032A/en unknown
- 1993-10-06 EP EP93923306A patent/EP0664836B1/en not_active Expired - Lifetime
- 1993-10-06 RU RU95110871/13A patent/RU95110871A/ru unknown
- 1993-10-06 WO PCT/US1993/009592 patent/WO1994008052A1/en not_active Application Discontinuation
- 1993-10-06 PL PL93308294A patent/PL308294A1/xx unknown
- 1993-10-06 ES ES93923306T patent/ES2083299T3/es not_active Expired - Lifetime
- 1993-10-06 DK DK93923306.0T patent/DK0664836T3/da active
- 1993-10-06 CA CA002146365A patent/CA2146365A1/en not_active Abandoned
- 1993-10-06 KR KR1019950701316A patent/KR950704508A/ko not_active Application Discontinuation
- 1993-10-06 AU AU53241/94A patent/AU670843B2/en not_active Ceased
- 1993-10-06 BR BR9307192A patent/BR9307192A/pt not_active Application Discontinuation
- 1993-10-06 CZ CZ95850A patent/CZ85095A3/cs unknown
-
1995
- 1995-03-29 US US08/412,913 patent/US5652103A/en not_active Expired - Fee Related
- 1995-04-05 NO NO951328A patent/NO951328L/no unknown
- 1995-04-05 FI FI951612A patent/FI951612A/fi not_active Application Discontinuation
-
1996
- 1996-05-15 GR GR950403607T patent/GR3019910T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
NO951328L (no) | 1995-05-22 |
AU5324194A (en) | 1994-04-26 |
WO1994008052A1 (en) | 1994-04-14 |
HU9500993D0 (en) | 1995-06-28 |
ATE134220T1 (de) | 1996-02-15 |
DE69301570T2 (de) | 1996-06-27 |
BR9307192A (pt) | 1999-03-30 |
AU670843B2 (en) | 1996-08-01 |
US5652103A (en) | 1997-07-29 |
NZ257032A (en) | 1997-06-24 |
KR950704508A (ko) | 1995-11-20 |
EP0664836B1 (en) | 1996-02-14 |
NO951328D0 (no) | 1995-04-05 |
CA2146365A1 (en) | 1994-04-14 |
FI951612A0 (fi) | 1995-04-05 |
JPH08504571A (ja) | 1996-05-21 |
DK0664836T3 (da) | 1996-03-11 |
RU95110871A (ru) | 1997-06-10 |
FI951612A (fi) | 1995-05-10 |
US5403709A (en) | 1995-04-04 |
HUT72988A (en) | 1996-06-28 |
PL308294A1 (en) | 1995-07-24 |
EP0664836A1 (en) | 1995-08-02 |
GR3019910T3 (en) | 1996-08-31 |
CZ85095A3 (en) | 1995-12-13 |
DE69301570D1 (de) | 1996-03-28 |
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