ES2080759T3 - Produccion de proteinas o polipeptidos fusionados. - Google Patents

Produccion de proteinas o polipeptidos fusionados.

Info

Publication number
ES2080759T3
ES2080759T3 ES89850031T ES89850031T ES2080759T3 ES 2080759 T3 ES2080759 T3 ES 2080759T3 ES 89850031 T ES89850031 T ES 89850031T ES 89850031 T ES89850031 T ES 89850031T ES 2080759 T3 ES2080759 T3 ES 2080759T3
Authority
ES
Spain
Prior art keywords
polypeptide
recombinant vector
fusion protein
host
dna sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89850031T
Other languages
English (en)
Inventor
Per-Ake Nygren
Lars Abrahmsen
Mathias Uhlen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of ES2080759T3 publication Critical patent/ES2080759T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
    • C07K14/445—Plasmodium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

UN METODO PARA PRODUCIR PROTEINA O POLIPEPTIDO DE FUSION CAPAZ DE UNION SELECTIVA A ALBUMINA DE SUERO, CARACTERIZADO POR LAS ETAPAS: A) CONSTRUIR UN VECTOR RECOMBINANTE QUE COMPRENDE UNA PRIMERA SECUENCIA DE ADN CODIFICANTE DE UN FRAGMENTO DE POLIPEPTIDO QUE UNE ALBUMINA DE SUERO Y ENLAZADO OPERATIVAMENTE A UNA SEGUNDA SECUENCIA DE ADN CODIFICANTE DE UNA PROTEINA O POLIPEPTIDO DESEADO; B) TRANSFORMAR UN ANFITRION COMPATIBLE CON DICHO VECTOR RECOMBINANTE TAL QUE LA SECUENCIA DE ADN COMBINADA QUE CODIFICA DICHA PROTEINA O POLIPEPTIDO DE FUSION, PUEDE SER EXPRESADA POR EL ANFITRION Y CULTIVAR EL ANFITRION TRANSFORMADO ENUN MEDIO DE CRECIMIENTO CONVENIENTE PARA PRODUCIR DICHO PROTEINA O POLIPEPTIDO DE FUSION; Y C) AISLAR DICHA PROTEINA O POLIPEPTIDO DE FUSION; UN VECTOR RECOMBINANTE CAPAZ DE REPLICACION EN UN MICROORGANISMO; UN ORGANISMO ANFITRION TRANSFORMADO POR TAL VECTOR RECOMBINANTE; Y PROTEINAS DE FUSION O POLIPE''PTIDOS PRODUCIDOS SEGUN TAL METODO.
ES89850031T 1988-02-05 1989-02-02 Produccion de proteinas o polipeptidos fusionados. Expired - Lifetime ES2080759T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE8800378A SE501169C2 (sv) 1988-02-05 1988-02-05 Framställning av serumalbuminbindande fusionsproteiner vilka innehåller protein G-domäner

Publications (1)

Publication Number Publication Date
ES2080759T3 true ES2080759T3 (es) 1996-02-16

Family

ID=20371279

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89850031T Expired - Lifetime ES2080759T3 (es) 1988-02-05 1989-02-02 Produccion de proteinas o polipeptidos fusionados.

Country Status (7)

Country Link
EP (1) EP0327522B1 (es)
JP (1) JP3026812B2 (es)
AT (1) ATE131494T1 (es)
DE (1) DE68925044T2 (es)
ES (1) ES2080759T3 (es)
GR (1) GR3018617T3 (es)
SE (1) SE501169C2 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2062759T3 (es) * 1990-01-24 1994-12-16 Upjohn Co Procedimiento de purificacion de polipeptidos recombinantes.
US5595887A (en) * 1990-07-16 1997-01-21 Bionebraska, Inc. Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
DE4037837A1 (de) * 1990-11-28 1992-06-04 Behringwerke Ag Zellfreie rezeptorbindungsteste, ihre herstellung und verwendung
FR2726471B1 (fr) * 1994-11-07 1997-01-31 Pf Medicament Procede pour ameliorer l'immunogenicite d'un compose immunogene ou d'un haptene et application a la preparation de vaccins
GB9509336D0 (en) * 1995-05-09 1995-06-28 Dynal As Chemical method
GB9716790D0 (en) 1997-08-07 1997-10-15 Creative Peptides Sweden Ab Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof
EP1900754A1 (en) * 2006-09-18 2008-03-19 Institut National De La Sante Et De La Recherche Medicale (Inserm) Fusion proteins comprising two lgG binding domains of streptococcal protein G

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE8300693L (sv) * 1983-02-09 1984-08-10 Sven Lofdahl Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta
SE459662B (sv) * 1986-03-21 1989-07-24 Pharmacia Ab Hybrid-dna-molekyl som kodar foer ett protein med samma igg specificitet som protein g, plasmid innehaallande densamma samt foerfarande foer framstaellning av proteinet

Also Published As

Publication number Publication date
SE8800378L (sv) 1989-08-06
SE8800378D0 (sv) 1988-02-05
EP0327522A2 (en) 1989-08-09
JPH025887A (ja) 1990-01-10
GR3018617T3 (en) 1996-04-30
SE501169C2 (sv) 1994-11-28
JP3026812B2 (ja) 2000-03-27
EP0327522B1 (en) 1995-12-13
ATE131494T1 (de) 1995-12-15
DE68925044D1 (de) 1996-01-25
DE68925044T2 (de) 1996-05-09
EP0327522A3 (en) 1990-07-04

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