ES2062759T3 - Procedimiento de purificacion de polipeptidos recombinantes. - Google Patents

Procedimiento de purificacion de polipeptidos recombinantes.

Info

Publication number
ES2062759T3
ES2062759T3 ES91901985T ES91901985T ES2062759T3 ES 2062759 T3 ES2062759 T3 ES 2062759T3 ES 91901985 T ES91901985 T ES 91901985T ES 91901985 T ES91901985 T ES 91901985T ES 2062759 T3 ES2062759 T3 ES 2062759T3
Authority
ES
Spain
Prior art keywords
recombinant polypeptide
purification procedure
polypeptide purification
fusion protein
desired polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91901985T
Other languages
English (en)
Inventor
Satish K Sharma
David B Evans
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pharmacia and Upjohn Co
Original Assignee
Upjohn Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Upjohn Co filed Critical Upjohn Co
Application granted granted Critical
Publication of ES2062759T3 publication Critical patent/ES2062759T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE PRESENTA UN METODO PARA PURIFICAR UN POLIPEPTIDO DESEADO Y UN EQUIPO PARA PRACTICAR LA PURIFICACION. EL METODO COMPRENDE LA PRODUCCION DE UNA PROTEINA DE FUSION QUE COMPRENDA UN POLIPEPTIDO DESEADO UNIDO A UN PEPTIDO DE AFINIDAD QUE CONTENGA UNA ZONA DE DISOCIACION DE LA ENDOPEPTIDASA. SE USA UNA ENDOPEPTIDASA INMOVILIZADA PARA SEPARAR LAS PROTEINAS DE FUSION DE LAS IMPUREZAS Y DIVIDIR LAS PROTEINAS DE FUSION EN SUS PARTES SUBSTITUYENTES.
ES91901985T 1990-01-24 1991-01-11 Procedimiento de purificacion de polipeptidos recombinantes. Expired - Lifetime ES2062759T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US46872490A 1990-01-24 1990-01-24

Publications (1)

Publication Number Publication Date
ES2062759T3 true ES2062759T3 (es) 1994-12-16

Family

ID=23860985

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91901985T Expired - Lifetime ES2062759T3 (es) 1990-01-24 1991-01-11 Procedimiento de purificacion de polipeptidos recombinantes.

Country Status (8)

Country Link
EP (1) EP0511978B1 (es)
JP (1) JP3188264B2 (es)
AT (1) ATE103292T1 (es)
AU (1) AU7166891A (es)
DE (1) DE69101486T2 (es)
DK (1) DK0511978T3 (es)
ES (1) ES2062759T3 (es)
WO (1) WO1991011454A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1245385B (it) * 1991-03-28 1994-09-20 Eniricerche Spa Procedimento migliorato per la preparazione di polipeptidi maturi
US6107059A (en) * 1992-04-29 2000-08-22 Affymax Technologies N.V. Peptide library and screening method
WO1994006469A1 (en) * 1992-09-18 1994-03-31 La Jolla Institute For Allergy And Immunology Hiv fusion polypeptide
US5563046A (en) * 1993-08-02 1996-10-08 Celtrix Pharmaceuticals, Inc. Fusion polypeptides and proteins
US5914254A (en) * 1993-08-02 1999-06-22 Celtrix Pharmaceuticals, Inc. Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
US5780285A (en) * 1995-03-03 1998-07-14 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing dibasic residues
US5837516A (en) * 1995-03-03 1998-11-17 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing basic residues
US7531325B2 (en) 1997-04-25 2009-05-12 Sembiosys Genetics Inc. Method for cleavage of fusion proteins
DK0977873T3 (da) 1997-04-25 2007-02-05 Sembiosys Genetics Inc Fremgangsmåde til spaltning af fusionsproteiner
US6068993A (en) * 1997-07-02 2000-05-30 Biobras Sa Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
US20170139503A1 (en) * 2014-06-12 2017-05-18 Mitsubishi Paper Mills Limited Optically transparent conductive material

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3410437A1 (de) * 1984-03-22 1985-09-26 Bayer Ag, 5090 Leverkusen Verfahren zur herstellung von proteinen
ATE46188T1 (de) * 1984-05-24 1989-09-15 Merck & Co Inc Positionsspezifische proteolyse mittels renin.
EP0244147A3 (en) * 1986-04-28 1989-04-05 Merck & Co. Inc. Purification process for hybrid proteins
US4851341A (en) * 1986-12-19 1989-07-25 Immunex Corporation Immunoaffinity purification system
SE501169C2 (sv) * 1988-02-05 1994-11-28 Aake Nygren Framställning av serumalbuminbindande fusionsproteiner vilka innehåller protein G-domäner

Also Published As

Publication number Publication date
EP0511978A1 (en) 1992-11-11
ATE103292T1 (de) 1994-04-15
JP3188264B2 (ja) 2001-07-16
EP0511978B1 (en) 1994-03-23
AU7166891A (en) 1991-08-21
DE69101486D1 (de) 1994-04-28
DE69101486T2 (de) 1994-08-04
WO1991011454A1 (en) 1991-08-08
JPH05503631A (ja) 1993-06-17
DK0511978T3 (da) 1994-08-01

Similar Documents

Publication Publication Date Title
ATE171216T1 (de) Expression und reinigung von rekombinantem, löslichem gewebefaktor
KR920701452A (ko) 프라세싱 부위에 인접한 음전하를 띤 아미노산을 함유하는 이스트 프라세싱 시스템
ES2035920T3 (es) Aerolizacion de agente proteico terapeutico.
ATE174628T1 (de) Expression rekombinanter fusionsproteine in attenuierten bakterien
DE69526636D1 (de) Gereinigte hepatitis-c-virus hüllproteine zur diagnostischen und therapeutischen verwendung
ES2076521T3 (es) Procedimiento para la disociacion enzimatica de proteinas recombinantes mediando utilizacion de proteasa de iga.
DE69210645D1 (de) Verfahren zur Herstellung von cysteinfreien Peptiden
ES2062759T3 (es) Procedimiento de purificacion de polipeptidos recombinantes.
EP0269455A3 (en) Highly purified fused protein comprising human ige fc fragment and production thereof
NO901769D0 (no) Fremgangsmaate for rensing av protein.
ATE278705T1 (de) Verfahren zur reinigung von proteinen
DE69319831D1 (de) Methode zur Sequenzanalyse C-terminaler Peptidfragmente
NO843750L (no) Immuninterferon og fremgangsmaate ved fremstilling derav
DE59006998D1 (de) Verfahren zur herstellung von hirudin.
ATE253592T1 (de) Verfahren zur reaktivierung von gereinigten membranproteinen durch einfrieren
KR890017357A (ko) 면연친화력 크로마토그래피를 사용한 재조합 프리(pre)-S2-베타갈락토시다제 융합단백질로부터의 프리-S2의 분리 및 정제방법
SE9303822D0 (sv) Method for purification

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 511978

Country of ref document: ES