ES2080755T3 - Separacion de enzimas termoestables. - Google Patents
Separacion de enzimas termoestables.Info
- Publication number
- ES2080755T3 ES2080755T3 ES89313157T ES89313157T ES2080755T3 ES 2080755 T3 ES2080755 T3 ES 2080755T3 ES 89313157 T ES89313157 T ES 89313157T ES 89313157 T ES89313157 T ES 89313157T ES 2080755 T3 ES2080755 T3 ES 2080755T3
- Authority
- ES
- Spain
- Prior art keywords
- thermostable enzyme
- unwanted contaminants
- enzyme
- inactivate
- adduct
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
UN ENZIMA TERMOESTABLE LIBRE DE CONTAMINANTES NO DESEADOS ESTA PROTEGIDO POR: A) UNA CELULA HUESPED CREADA PARA EXPRESAR UN GENE DE ENCODIFICACION DE ENZIMAS TERMOESTABLES HETEROLOGOS. B) EL CULTIVO DE UNA CELULA HUESPED MESOFILICA Y LA PRODUCCION DEL ENZIMA EN UNA MEZCLA QUE COMPENDE CONTAMINANTES NO DESEADOS; Y C) LA PURIFICACION DEL ENZIMA POR UN METODO QUE INCLUYE EL CALENTAMIENTO DEL ENZIMA Y LOS CONTAMINANTES NO DESEADOS A UNA TEMPERATURA SUFICIENTE COMO PARA ENACTIVAR DICHO CONTAMINANTE PERO SIN INACTIVAR EL ENZIMA. UN METODO DE ENSAYO PARA LIGAR ACIDOS NUCLEICOS INCLUYE LA FORMACION DE UN ADUCTO ADENILICO. ESTE ULTIMO METODO ES USADO PARA VISUALIZAR UNA CANTIDAD DE TRANSFORMANTES QUE PERMITE EXPRESAR EN ENLACE DE ACIDO NUCLEICO.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US28512888A | 1988-12-16 | 1988-12-16 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2080755T3 true ES2080755T3 (es) | 1996-02-16 |
Family
ID=23092846
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES89313157T Expired - Lifetime ES2080755T3 (es) | 1988-12-16 | 1989-12-15 | Separacion de enzimas termoestables. |
Country Status (10)
Country | Link |
---|---|
EP (1) | EP0373962B1 (es) |
JP (1) | JP2854902B2 (es) |
KR (1) | KR900009982A (es) |
AT (1) | ATE127843T1 (es) |
AU (1) | AU627809B2 (es) |
CA (1) | CA2005667A1 (es) |
DE (1) | DE68924251T2 (es) |
ES (1) | ES2080755T3 (es) |
GR (1) | GR3017991T3 (es) |
TW (1) | TW203626B (es) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2774192B2 (ja) * | 1989-12-22 | 1998-07-09 | エフ.ホフマン ― ラ ロシュ アーゲー | サーマス サーモフィルス dnaポリメレースの発現ベクターと精製に関する方法 |
JP3889807B2 (ja) * | 1990-05-03 | 2007-03-07 | コーネル・リサーチ・ファンデーション・インコーポレイテッド | 遺伝病を検出するための熱安定リガーゼによるdna増幅系 |
JPH0824571B2 (ja) * | 1991-08-20 | 1996-03-13 | 工業技術院長 | 耐熱性制限酵素の調製法 |
US5700672A (en) * | 1992-07-23 | 1997-12-23 | Stratagene | Purified thermostable pyrococcus furiousus DNA ligase |
US6180338B1 (en) | 1992-08-04 | 2001-01-30 | Beckman Coulter, Inc. | Method, reagent and kit for the detection and amplification of nucleic acid sequences |
US6207368B1 (en) | 1992-08-04 | 2001-03-27 | Beckman Coulter, Inc. | Methods and reagents for controlling chain extension and ligation chain reactions |
US5491086A (en) * | 1993-05-14 | 1996-02-13 | Hoffmann-La Roche Inc. | Purified thermostable nucleic acid polymerase and DNA coding sequences from pyrodictium species |
US7014994B1 (en) | 1999-03-19 | 2006-03-21 | Cornell Research Foundation,Inc. | Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process |
US8286311B2 (en) | 2008-10-31 | 2012-10-16 | Jisook Paik | Buckle |
GB0906643D0 (en) * | 2009-04-17 | 2009-06-03 | Wilson Stuart M | Detection of bacteria and fungi |
WO2019006723A1 (en) * | 2017-07-06 | 2019-01-10 | Cathay R&D Center Co., Ltd. | HETEROLOGOUS EXPRESSION OF THERMOPHILIC DECARBOXYLASE LYSINE AND USES THEREOF |
DE102021124566A1 (de) | 2021-09-22 | 2023-03-23 | Universität Duisburg-Essen, Körperschaft des öffentlichen Rechts | Verfahren zur rekombinanten herstellung von polypeptiden |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1338457C (en) * | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Purified thermostable enzyme |
IL88923A (en) * | 1988-01-12 | 1995-07-31 | Hoffmann La Roche | Gene encoding a thermostable dna polymerase from thermus aquaticus said dna polymerase and its purification |
-
1989
- 1989-12-13 AU AU46715/89A patent/AU627809B2/en not_active Ceased
- 1989-12-15 EP EP89313157A patent/EP0373962B1/en not_active Expired - Lifetime
- 1989-12-15 DE DE68924251T patent/DE68924251T2/de not_active Expired - Fee Related
- 1989-12-15 AT AT89313157T patent/ATE127843T1/de not_active IP Right Cessation
- 1989-12-15 ES ES89313157T patent/ES2080755T3/es not_active Expired - Lifetime
- 1989-12-15 CA CA002005667A patent/CA2005667A1/en not_active Abandoned
- 1989-12-15 KR KR1019890018619A patent/KR900009982A/ko not_active Application Discontinuation
- 1989-12-16 JP JP1327063A patent/JP2854902B2/ja not_active Expired - Lifetime
-
1990
- 1990-03-01 TW TW079101599A patent/TW203626B/zh active
-
1995
- 1995-11-08 GR GR950403100T patent/GR3017991T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
EP0373962A2 (en) | 1990-06-20 |
AU4671589A (en) | 1990-06-21 |
EP0373962B1 (en) | 1995-09-13 |
DE68924251D1 (de) | 1995-10-19 |
EP0373962A3 (en) | 1991-08-21 |
KR900009982A (ko) | 1990-07-06 |
JP2854902B2 (ja) | 1999-02-10 |
GR3017991T3 (en) | 1996-02-29 |
TW203626B (es) | 1993-04-11 |
ATE127843T1 (de) | 1995-09-15 |
CA2005667A1 (en) | 1990-06-16 |
JPH02257880A (ja) | 1990-10-18 |
DE68924251T2 (de) | 1996-04-25 |
AU627809B2 (en) | 1992-09-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ES2080755T3 (es) | Separacion de enzimas termoestables. | |
IL61982A (en) | Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes | |
DK496485D0 (da) | Transformation af gaer-stammer af slaegten pichia | |
AU1591888A (en) | Element and method for performing biological assays accurately, rapidly and simply | |
ES2059478T3 (es) | Co-expresion en celulas eucarioticas. | |
EP0236985A3 (en) | Method for the photometric determination of protein c and/or protein s activity | |
JPS57139097A (en) | Method of cloning gene coding heat-resistant alpha-amylase to colibacillus and bacillus subtilis | |
EP0128018A3 (en) | Molecular genetic probe, and method of forming same, assay technique and kit using said molecular genetic probe | |
DK359487D0 (da) | Fremgangsmaade til udvirkning af foroeget ekspression af rekombinant faktor viii i en vaertscelle | |
ATE123304T1 (de) | Durch rekombinante dns-technologie konstruierte amylolytische enzymproduzierende mikroorganismen und ihre verwendung in gärungsverfahren. | |
IL75584A0 (en) | Production of useful substances by using genetic engineering techniques | |
DE69031042D1 (de) | Fermentationsprozess zur herstellung von pyrimidin | |
DE69029663D1 (de) | Verfahren zur endoproteolytischen bearbeitung von (vorläufer)proteinen und zur (mikro)biologischen herstellung | |
ATE42259T1 (de) | Biologische flockung von mineralschlaemmen. | |
DK340882A (da) | Fremgangsmaade til fremstilling af sur protease og mutanter af slaegten rhizopus, som danner denne | |
EP0825258A4 (en) | METHOD FOR DETERMINING 1,5-ANHYDROGLUCITOL | |
CA1312566C (en) | Method for increasing the expression of polypeptides in recombinant cell culture | |
ES8600400A1 (es) | Un procedimiento para la produccion de histidina | |
AU5381086A (en) | Fluorescence polarization assay for amylase | |
EP0311456A3 (en) | Monoclonal antibody to okadaic acids, process for producing the monoclonal antibody, assay reagent for assaying okadaic acids using the monoclonal antibody, and assay method | |
DE3878410D1 (de) | Verfahren zur erhoehung von gen-expression. | |
ATE49058T1 (de) | Verfahren und reagenz zur spezifischen bestimmung von pankreas-alpha-amylase. | |
Lunan et al. | The isolation of C14-labeled pyridoxamine from Candida utilis ATCC 9950 | |
JPS54132291A (en) | Novel bioreactor | |
Fripiat et al. | A strategy for the evaluation of methane production from different types of substrate biomass. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
Ref document number: 373962 Country of ref document: ES |