ES2073409T3 - Dna conteniendo el codigo de la sintetasa del acv. - Google Patents
Dna conteniendo el codigo de la sintetasa del acv.Info
- Publication number
- ES2073409T3 ES2073409T3 ES88311655T ES88311655T ES2073409T3 ES 2073409 T3 ES2073409 T3 ES 2073409T3 ES 88311655 T ES88311655 T ES 88311655T ES 88311655 T ES88311655 T ES 88311655T ES 2073409 T3 ES2073409 T3 ES 2073409T3
- Authority
- ES
- Spain
- Prior art keywords
- acv
- penicillin
- gene
- hosts
- expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Compounds Of Unknown Constitution (AREA)
Abstract
Un procedimiento para la transformación de una célula huésped con un vector recombinante, el cual, comprende el mezclado conjunto del huésped y del vector recombinante bajo condiciones de transformación convencionales, caracterizado porque el vector recombinante comprende DNA, el cual, comprende, esencialmente, un gen que contiene el código de una enzima sintetasa capaz de generar (L-aminoadipil)-L-cisteinil-D-valina obtenible a partir de Penicillium chrysogenum, mediante: a) la construcción de una biblioteca genómica a partir de los fragmentos de DNA cromosómicos del P. chrysogenum, b) la realización de uno o más experimentos de hibridación con el fin de seleccionar, a partir de dicha biblioteca, los clones que contengan el DNA de la invención y c) el aislamiento del DNA de la invención, conteniendo dicho DNA la configuración de los sitios de restricción mostrados en la Figura 6(b).
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB878728811A GB8728811D0 (en) | 1987-12-09 | 1987-12-09 | Novel substance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2073409T3 true ES2073409T3 (es) | 1995-08-16 |
Family
ID=10628254
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES88311655T Expired - Lifetime ES2073409T3 (es) | 1987-12-09 | 1988-12-09 | Dna conteniendo el codigo de la sintetasa del acv. |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0320272B1 (es) |
| JP (1) | JP2970918B2 (es) |
| AT (1) | ATE122718T1 (es) |
| DE (1) | DE3853811T2 (es) |
| ES (1) | ES2073409T3 (es) |
| GB (1) | GB8728811D0 (es) |
| GR (1) | GR3017073T3 (es) |
| WO (1) | WO1989005350A1 (es) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5108918A (en) * | 1988-08-11 | 1992-04-28 | Gist-Brocades | Method for identifying and using biosynthetic genes for enhanced production of secondary metabolites |
| FI104264B (fi) * | 1988-08-11 | 1999-12-15 | Dsm Ip Assets Bv | Menetelmä sekundääristen metaboliittien tuoton parantamiseksi käyttämällä rykelmöityjä biosynteettisiä geenejä |
| US5882879A (en) * | 1990-02-28 | 1999-03-16 | Gist-Brocades, N.V. | Method for influencing β-lactam antibiotic production and for isolation of large quantities of ACV synthetase |
| ATE169059T1 (de) * | 1990-02-28 | 1998-08-15 | Gist Brocades Nv | Verfahren zur beeinflussung der produktion von beta-lactam antibiotika und zur isolierung grosser mengen von acv-synthetase |
| US6322998B1 (en) | 1990-06-01 | 2001-11-27 | Eli Lilly And Company | Recombinant DNA compounds that encode ACV synthetase activity of Cephalosporium acremonium |
| US5589571A (en) * | 1994-10-28 | 1996-12-31 | Cor Therapeutics, Inc. | Process for production of inhibited forms of activated blood factors |
| CN101098963A (zh) * | 2004-12-10 | 2008-01-02 | 帝斯曼知识产权资产管理有限公司 | 通过经遗传修饰的非天然生产微生物来生产β-内酰胺 |
| US20100048938A1 (en) * | 2006-06-22 | 2010-02-25 | Marco Alexander Van Den Berg | Fermentation of pravastatin |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59111699A (ja) * | 1982-12-17 | 1984-06-27 | 富士通株式会社 | 話者認識方式 |
| CA1309673C (en) * | 1985-04-22 | 1992-11-03 | Stephen Wyatt Queener | Recombinant dna expression vectors and dna compounds which encode isopenicillin n synthetase |
| US4892819A (en) * | 1985-11-25 | 1990-01-09 | Eli Lilly And Company | Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum |
| EP0233715B1 (en) * | 1986-01-31 | 1994-05-25 | BEECHAM GROUP plc | Isolation and expression of genes involved in the biosynthesis of beta-lactams |
| CA1327170C (en) * | 1987-03-04 | 1994-02-22 | Stephen Wyatt Queener | Recombinant dna expression vectors and dna compounds that encode deacetoxycephalosporin c synthetase and deacetylcephalosporin c synthetase |
| GB2208077A (en) * | 1987-04-24 | 1989-02-22 | Antibioticos Sa | Isopenicillin n synthetase gene derived from aspergillus nidulans |
-
1987
- 1987-12-09 GB GB878728811A patent/GB8728811D0/en active Pending
-
1988
- 1988-12-09 WO PCT/GB1988/001083 patent/WO1989005350A1/en unknown
- 1988-12-09 ES ES88311655T patent/ES2073409T3/es not_active Expired - Lifetime
- 1988-12-09 JP JP1500399A patent/JP2970918B2/ja not_active Expired - Lifetime
- 1988-12-09 EP EP88311655A patent/EP0320272B1/en not_active Expired - Lifetime
- 1988-12-09 DE DE3853811T patent/DE3853811T2/de not_active Expired - Lifetime
- 1988-12-09 AT AT88311655T patent/ATE122718T1/de not_active IP Right Cessation
-
1995
- 1995-08-09 GR GR950402201T patent/GR3017073T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE3853811T2 (de) | 1995-10-12 |
| JP2970918B2 (ja) | 1999-11-02 |
| GB8728811D0 (en) | 1988-01-27 |
| EP0320272A1 (en) | 1989-06-14 |
| WO1989005350A1 (en) | 1989-06-15 |
| ATE122718T1 (de) | 1995-06-15 |
| DE3853811D1 (de) | 1995-06-22 |
| GR3017073T3 (en) | 1995-11-30 |
| JPH02502606A (ja) | 1990-08-23 |
| EP0320272B1 (en) | 1995-05-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Smith et al. | Cloning and heterologous expression of the penicillin biosynthetic gene cluster from Penicillium chrysogenum | |
| ES2302379T3 (es) | Produccion in vivo mejorada de cefalosporina. | |
| RU2002130702A (ru) | Новый биологический способ получения 7-ацк и 7-адац | |
| Queener | Molecular biology of penicillin and cephalosporin biosynthesis | |
| Martin et al. | Molecular genetics of penicillin and cephalosporin antibiotic biosynthesis | |
| DE69227494D1 (de) | Neues biologisches Verfahren zur Herstellung von 7-ADCA | |
| ES2073409T3 (es) | Dna conteniendo el codigo de la sintetasa del acv. | |
| HUT46068A (en) | Gene technological process for producing trypsin inhibitor produced in pancreas by means of recombinant host organism | |
| KR910018550A (ko) | 페니실린 g 아실라아제, 이 효소를 코드화한 유전자 및 이 효소의 제조 방법 | |
| ES2086386T3 (es) | Procedimiento para producir compuestos de 7-aminocefem o sus sales. | |
| KR900700610A (ko) | β-락탐 화합물의 생성을 위한 유전자 및 유전자 생성물 | |
| DE69121983D1 (de) | Verfahren zur Herstellung von Ascorbinsäure-2-Phosphat | |
| GR3031739T3 (en) | Improved process for preparing cephalosporins. | |
| ATE40408T1 (de) | Biosynthese von unnatuerlichen cephalosporinen. | |
| EP0064681A3 (en) | Novel recombinant dna | |
| ES8800361A1 (es) | Un metodo para producir un interferon-gamma | |
| Ghosh | Strategies for manipulation of genes in microbes for production of β-lactam antibiotics | |
| Liras Padín et al. | Biochemistry and genetics of Cephamycin C production by Nocardia lactamdurans | |
| JPS6451083A (en) | Production of acidic urease |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
Ref document number: 320272 Country of ref document: ES |