ES2065038T3 - Procedimiento para la determinacion de la actividad de polimerasa. - Google Patents

Procedimiento para la determinacion de la actividad de polimerasa.

Info

Publication number
ES2065038T3
ES2065038T3 ES91910145T ES91910145T ES2065038T3 ES 2065038 T3 ES2065038 T3 ES 2065038T3 ES 91910145 T ES91910145 T ES 91910145T ES 91910145 T ES91910145 T ES 91910145T ES 2065038 T3 ES2065038 T3 ES 2065038T3
Authority
ES
Spain
Prior art keywords
polymerase
activity
determination
procedure
immobilizable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91910145T
Other languages
English (en)
Inventor
Josef Eberle
Rudolf Seibl
Christoph Kessler
Bernhard Konig
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2065038T3 publication Critical patent/ES2065038T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • AIDS & HIV (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

PROCEDIMIENTO PARA LA DETERMINACION DE LA ACTIVIDAD DE LA POLIMERASA, POR MEDIO DE LA INCUBACION DE POLIMERASA, CON UN ACIDO NUCLEICO TEMPLAT, UN TRIFOSFATO DE MONONUCLEOSIDO DETECTABLE Y UN TRIFOSFATO DE NUCLEOSIDO INMOVILIZABLE, ENLACE DE LOS NUCLEOTIDOS INMOVILIZABLES A UNA FASE SOLIDA, Y COMPROBACION DE LOS NUCLEOTIDOS ENLAZADOS DETECTABLES Y LOS TEST BASADOS EN ESTOS.
ES91910145T 1990-08-31 1991-05-31 Procedimiento para la determinacion de la actividad de polimerasa. Expired - Lifetime ES2065038T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4027616A DE4027616A1 (de) 1990-08-31 1990-08-31 Verfahren zur bestimmung von polymerase-aktivitaet

Publications (1)

Publication Number Publication Date
ES2065038T3 true ES2065038T3 (es) 1995-02-01

Family

ID=6413331

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91910145T Expired - Lifetime ES2065038T3 (es) 1990-08-31 1991-05-31 Procedimiento para la determinacion de la actividad de polimerasa.

Country Status (8)

Country Link
US (2) US5413906A (es)
EP (1) EP0547056B1 (es)
JP (1) JP2511221B2 (es)
AT (1) ATE111961T1 (es)
DE (2) DE4027616A1 (es)
DK (1) DK0547056T3 (es)
ES (1) ES2065038T3 (es)
WO (1) WO1992004467A1 (es)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6316182B1 (en) * 1992-01-15 2001-11-13 Nen Life Science Products, Inc. Detection of reverse transcriptase by DNA hybridization assay
WO1993023560A1 (de) * 1992-05-11 1993-11-25 Schuepbach Joerg Verfahren zum nachweis reverser transcriptase
US5744306A (en) * 1993-04-19 1998-04-28 Emory University Methods for nucleic acid detection, sequencing, and cloning using exonuclease
US5635349A (en) * 1994-12-02 1997-06-03 Tularik, Inc. High-throughput screening assay for inhibitors of nucleic acid polymerases
US6096508A (en) * 1995-08-16 2000-08-01 Kirkegaard & Perry Laboratoies, Inc. Method of reducing background in biotin-based assays
US6043038A (en) * 1998-03-31 2000-03-28 Tularik, Inc. High-throughput screening assays for modulators of primase activity
AU7986398A (en) * 1997-06-25 1999-01-04 Tularik Inc. High-throughput screening assays for modulators of primase activity
ES2153807T3 (es) * 1997-08-08 2005-06-16 Biomerieux B.V. Secuencias de acido nucleico que se pueden utilizar como cebadores y sondas en la amplificacion y deteccion de todos los subtipos de vih-1.
US6635418B2 (en) * 1998-02-13 2003-10-21 Igen International, Inc. Assay methods for nucleic acid in a sample
AUPP249298A0 (en) * 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
SK287538B6 (sk) 1998-03-20 2011-01-04 Commonwealth Scientific And Industrial Research Organisation Syntetický gén obsahujúci dispergovanú alebo cudzorodú deoxyribonukleovú molekulu, génový konštrukt obsahujúci tento syntetický gén a prípravok obsahujúci tento syntetický gén
US6423885B1 (en) 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
AUPR621501A0 (en) 2001-07-06 2001-08-02 Commonwealth Scientific And Industrial Research Organisation Delivery of ds rna
US7892563B2 (en) 2003-05-20 2011-02-22 Wyeth Holdings Corporation Methods for treatment of severe acute respiratory syndrome (SARS)
GB0318110D0 (en) * 2003-08-01 2003-09-03 Isaeo Ltd Methods and kits for detecting an enzyme capable of modifying a nucleic acid
US20050147965A1 (en) * 2003-12-31 2005-07-07 Zhong Zhandong D. Compositions and methods for detecting reverse transcriptase in a sample
BRPI0510811B1 (pt) * 2004-05-13 2018-12-26 Goel Anita métodos para amplificação de ácido nucléico, método de detecção de patógeno e métodos para execução de amplificação de ácido nucléico
JP2009534033A (ja) 2006-04-21 2009-09-24 ナノバイオシン，インコーポレイテッド 薬物発見のための単一分子プラットホーム：抗癌剤および抗ウイルス剤の発見を含む薬物発見のための方法および装置
MY161567A (en) * 2010-04-16 2017-04-28 Zeus Scientific Inc Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
US10933417B2 (en) 2013-03-15 2021-03-02 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
GB8509367D0 (en) * 1985-04-12 1985-05-15 Amersham Int Plc Nucleic acid hybridisation
US4851331A (en) * 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase
US4942122A (en) * 1987-02-24 1990-07-17 Research Education Institute, Inc. Aids prognosis test detecting the presence of antibodies inhibiting HIV reverse transcriptase
EP0322922A3 (en) * 1987-12-31 1990-06-06 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Detection of avian and mammalian retrovirus infections
DE3800644A1 (de) * 1988-01-12 1989-07-20 Boehringer Mannheim Gmbh Verfahren zum hochspezifischen nachweis von nukleinsaeuren in loesung
EP0392459B1 (en) * 1989-04-14 1995-08-09 Asahi Kasei Kogyo Kabushiki Kaisha Method for the measurement of reverse transcriptase by nonradioactive substance

Also Published As

Publication number Publication date
EP0547056B1 (de) 1994-09-21
DE59103063D1 (de) 1994-10-27
WO1992004467A1 (de) 1992-03-19
JPH05505942A (ja) 1993-09-02
EP0547056A1 (de) 1993-06-23
US5413906A (en) 1995-05-09
DK0547056T3 (da) 1995-03-20
ATE111961T1 (de) 1994-10-15
US5635350A (en) 1997-06-03
JP2511221B2 (ja) 1996-06-26
DE4027616A1 (de) 1992-03-05

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