ES2045113T3 - Un metodo para obtener una curva patron lineal en un inmunoensayo de tipo emparedado. - Google Patents
Un metodo para obtener una curva patron lineal en un inmunoensayo de tipo emparedado.Info
- Publication number
- ES2045113T3 ES2045113T3 ES88303904T ES88303904T ES2045113T3 ES 2045113 T3 ES2045113 T3 ES 2045113T3 ES 88303904 T ES88303904 T ES 88303904T ES 88303904 T ES88303904 T ES 88303904T ES 2045113 T3 ES2045113 T3 ES 2045113T3
- Authority
- ES
- Spain
- Prior art keywords
- antibody
- obtaining
- antigen
- sandwiched
- standard curve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/801—Electron dense compounds, e.g. ferritin
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/803—Stabe free radicals, e.g. spin immunoassay
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
- Y10S436/817—Steroids or hormones
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/82—Hepatitis associated antigens and antibodies
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/827—Lectins
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Endocrinology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
UN METODO PARA OBTENER UNA CURVA PATRON LINEAL REAL EN UN TIPO DE INMUNOENSAYO DE INTERCALACION, DONDE UN PRIMER ANTICUERPO UNIDO A UN SOPORTE INSOLUBLE Y UN SEGUNDO ANTICUERPO MARCADO NO ENLAZADO FORMAN COMPLEJO CON EL ANTIGENO CONTENIDO EN UNA MUESTRA DE ENSAYO PARA FORMAR UN COMPLEJO ANTICUERPO-ANTIGENO-ANTICUERPO MARCADO, QUE LUEGO SE DETECTA. SE AÑADEN A LA MEZCLA DE REACCION EL PRIMER ANTICUERPO NO MARCADO NO ENLAZADO Y/O EL SEGUNDO ANTICUERPO NO MARACADO NO ENLAZADO PARA RETIRAR EL EXCESO DE ANTIGENO DEL COMPLEJO PRODUCTO FINAL DESEADO, HACIENDO ASI AL ANTIGENO DE INTERES EL FACTOR LIMITANTE DE LA VELOCIDAD EN LA INMUNOREACCION TOTAL. ESTO DA LUGAR A UNA SEUDOREACCION DE PRIMER ORDEN QUE PRODUCE UNA CURVA PATRON LINEAL REAL.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/044,099 US4788138A (en) | 1987-04-30 | 1987-04-30 | Method to achieve a linear standard curve in a sandwich immunoassay |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2045113T3 true ES2045113T3 (es) | 1994-01-16 |
Family
ID=21930530
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES88303904T Expired - Lifetime ES2045113T3 (es) | 1987-04-30 | 1988-04-29 | Un metodo para obtener una curva patron lineal en un inmunoensayo de tipo emparedado. |
Country Status (11)
Country | Link |
---|---|
US (1) | US4788138A (es) |
EP (1) | EP0289335B1 (es) |
JP (1) | JP2568910B2 (es) |
AT (1) | ATE84879T1 (es) |
AU (1) | AU617959B2 (es) |
CA (1) | CA1294870C (es) |
DE (1) | DE3877617T2 (es) |
ES (1) | ES2045113T3 (es) |
FI (1) | FI90799C (es) |
NO (1) | NO175657C (es) |
WO (1) | WO1988008535A1 (es) |
Families Citing this family (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4891313A (en) * | 1988-01-21 | 1990-01-02 | Boehringer Manheim Corporation | Method for determination of a component of a sample |
WO1989010974A1 (en) * | 1988-05-11 | 1989-11-16 | Trustees Of The Sisters Of Charity Of Australia | Enzyme immunoassay system |
GB8812213D0 (en) * | 1988-05-24 | 1988-06-29 | Ares Serono Res & Dev Ltd | Method of assay |
JPH0754322B2 (ja) * | 1988-11-07 | 1995-06-07 | 三洋化成工業株式会社 | 免疫学的定量法および検査用試薬 |
US5939272A (en) * | 1989-01-10 | 1999-08-17 | Biosite Diagnostics Incorporated | Non-competitive threshold ligand-receptor assays |
FR2652900B1 (fr) * | 1989-10-11 | 1994-01-28 | Clonatec Sa | Procede d'analyse immunologique de type "sandwich" son application au depistage prenatal in vitro de la trisomie 21 et necessaire pour sa mise en óoeuvre. |
FR2657167B1 (fr) * | 1990-01-16 | 1992-05-07 | Clonatec Sa | Procede de depistage prenatal in vitro de la trisomie 21 fóoetale dans un echantillon biologique susceptible de contenir de l'hcg et necessaire pour sa mise en óoeuvre. |
TW274582B (es) * | 1992-08-03 | 1996-04-21 | Hoffmann La Roche | |
US5556759A (en) * | 1993-08-02 | 1996-09-17 | Beach; Peter G. | Assay and method for determining newborn risk for sudden infant death syndrome |
US5597700A (en) * | 1994-04-28 | 1997-01-28 | California Research, Llc | Method for detecting free insulin-like growth-factor-binding protein 1 and a test device for detecting the ruptures of fetal membranes using the above method |
DE69530043T2 (de) * | 1994-11-08 | 2003-10-16 | Tosoh Corp | Verfahren zur Bestimmung einer fluoreszierenden Substanz, sowie Verfahren zur Enzym-Aktivität-Bestimmung |
US5705353A (en) * | 1995-06-07 | 1998-01-06 | Beckman Instruments, Inc. | Method of reducing interferences in assays |
US6686165B2 (en) * | 1997-05-20 | 2004-02-03 | Erasmus Universiteit Rotterdam | Recognition of tumor-specific gene products in cancer |
US6284472B1 (en) | 1998-10-05 | 2001-09-04 | Dade Behring Inc. | Method for extending the range of an immunoassay |
US6905886B2 (en) * | 2000-08-11 | 2005-06-14 | Quest Diagnostics Investments Incorporated | Preservative solutions |
US6387711B1 (en) * | 2000-08-11 | 2002-05-14 | Quest Diagnostics Investments Incorporated | Liquid intact parathyroid hormone (PTH) standards |
US7935479B2 (en) | 2004-07-19 | 2011-05-03 | Cell Biosciences, Inc. | Methods and devices for analyte detection |
US7846676B2 (en) * | 2004-07-19 | 2010-12-07 | Cell Biosciences, Inc. | Methods and devices for analyte detection |
US20060292558A1 (en) * | 2004-07-19 | 2006-12-28 | Cell Biosciences Inc. | Methods and apparatus for protein assay diagnostics |
JP5073491B2 (ja) * | 2004-07-19 | 2012-11-14 | プロテインシンプル | 分析対象物の検出のための方法 |
WO2007035864A2 (en) | 2005-09-20 | 2007-03-29 | Cell Biosciences, Inc. | Electrophoresis standards, methods and kits |
US20080017512A1 (en) * | 2006-07-24 | 2008-01-24 | Bordunov Andrei V | Coatings for capillaries capable of capturing analytes |
US8956823B2 (en) * | 2007-08-20 | 2015-02-17 | Bio-Rad Laboratories, Inc. | Anti-antibody reagent |
WO2009126336A1 (en) * | 2008-04-11 | 2009-10-15 | Becton, Dickinson And Company | Methods of controlling the sensitivity and dynamic range of a homogeneous assay |
WO2013155634A1 (en) | 2012-04-19 | 2013-10-24 | Jiaqi Wu | Dual wavelength isoelectric focusing for determining drug load in antibody drug conjugates |
US9766206B2 (en) | 2013-09-27 | 2017-09-19 | ProteinSimple | Apparatus, systems, and methods for capillary electrophoresis |
JP6277099B2 (ja) | 2014-09-09 | 2018-02-07 | 富士フイルム株式会社 | 試薬キット、測定キット及び被検物質の測定方法。 |
CN111693690A (zh) * | 2020-07-05 | 2020-09-22 | 江苏拜明生物技术有限公司 | 改善一步法双抗夹心反应中hook效应及平台期的方法 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4016143A (en) * | 1971-12-07 | 1977-04-05 | Bayer Aktiengesellschaft | Polyurethanes based on aromatic polyamines |
US4376110A (en) * | 1980-08-04 | 1983-03-08 | Hybritech, Incorporated | Immunometric assays using monoclonal antibodies |
US4486530A (en) * | 1980-08-04 | 1984-12-04 | Hybritech Incorporated | Immunometric assays using monoclonal antibodies |
EP0101228B1 (en) * | 1982-08-06 | 1986-10-08 | International Institute Of Cellular And Molecular Pathology (Icp) | Particle agglutination assay of antigens |
US4659678A (en) * | 1982-09-29 | 1987-04-21 | Serono Diagnostics Limited | Immunoassay of antigens |
WO1985000663A1 (en) * | 1983-07-25 | 1985-02-14 | Beckman Instruments, Inc. | Immunometric assay using polyclonal and monoclonal antibodies and a kit for use therein |
US4595661A (en) * | 1983-11-18 | 1986-06-17 | Beckman Instruments, Inc. | Immunoassays and kits for use therein which include low affinity antibodies for reducing the hook effect |
US4690890A (en) * | 1984-04-04 | 1987-09-01 | Cetus Corporation | Process for simultaneously detecting multiple antigens using dual sandwich immunometric assay |
US4743542A (en) * | 1985-04-11 | 1988-05-10 | Ortho Diagnostic | Method for forestalling the hook effect in a multi-ligand immunoassay system |
-
1987
- 1987-04-30 US US07/044,099 patent/US4788138A/en not_active Expired - Fee Related
-
1988
- 1988-04-13 JP JP63505605A patent/JP2568910B2/ja not_active Expired - Lifetime
- 1988-04-13 AU AU19812/88A patent/AU617959B2/en not_active Ceased
- 1988-04-13 WO PCT/US1988/001277 patent/WO1988008535A1/en active IP Right Grant
- 1988-04-18 CA CA000564400A patent/CA1294870C/en not_active Expired - Lifetime
- 1988-04-29 DE DE8888303904T patent/DE3877617T2/de not_active Expired - Fee Related
- 1988-04-29 EP EP88303904A patent/EP0289335B1/en not_active Expired - Lifetime
- 1988-04-29 ES ES88303904T patent/ES2045113T3/es not_active Expired - Lifetime
- 1988-04-29 AT AT88303904T patent/ATE84879T1/de active
- 1988-12-28 FI FI886000A patent/FI90799C/fi not_active IP Right Cessation
- 1988-12-29 NO NO885808A patent/NO175657C/no unknown
Also Published As
Publication number | Publication date |
---|---|
ATE84879T1 (de) | 1993-02-15 |
EP0289335B1 (en) | 1993-01-20 |
NO885808L (no) | 1988-12-29 |
DE3877617D1 (de) | 1993-03-04 |
DE3877617T2 (de) | 1993-05-13 |
FI886000A (fi) | 1988-12-28 |
EP0289335A2 (en) | 1988-11-02 |
FI90799C (fi) | 1994-03-25 |
NO885808D0 (no) | 1988-12-29 |
AU1981288A (en) | 1988-12-02 |
NO175657C (no) | 1994-11-09 |
JPH01503177A (ja) | 1989-10-26 |
US4788138A (en) | 1988-11-29 |
AU617959B2 (en) | 1991-12-05 |
NO175657B (es) | 1994-08-01 |
WO1988008535A1 (en) | 1988-11-03 |
FI90799B (fi) | 1993-12-15 |
JP2568910B2 (ja) | 1997-01-08 |
CA1294870C (en) | 1992-01-28 |
EP0289335A3 (en) | 1988-12-21 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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