DE69530043T2 - Verfahren zur Bestimmung einer fluoreszierenden Substanz, sowie Verfahren zur Enzym-Aktivität-Bestimmung - Google Patents
Verfahren zur Bestimmung einer fluoreszierenden Substanz, sowie Verfahren zur Enzym-Aktivität-BestimmungInfo
- Publication number
- DE69530043T2 DE69530043T2 DE69530043T DE69530043T DE69530043T2 DE 69530043 T2 DE69530043 T2 DE 69530043T2 DE 69530043 T DE69530043 T DE 69530043T DE 69530043 T DE69530043 T DE 69530043T DE 69530043 T2 DE69530043 T2 DE 69530043T2
- Authority
- DE
- Germany
- Prior art keywords
- determining
- enzyme activity
- fluorescent substance
- determining enzyme
- fluorescent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000004190 Enzymes Human genes 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 title 1
- 239000000126 substance Substances 0.000 title 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
- G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
- G01N21/274—Calibration, base line adjustment, drift correction
- G01N21/276—Calibration, base line adjustment, drift correction with alternation of sample and standard in optical path
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP27374694 | 1994-11-08 |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69530043D1 DE69530043D1 (de) | 2003-04-30 |
DE69530043T2 true DE69530043T2 (de) | 2003-10-16 |
Family
ID=17532001
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69530043T Expired - Lifetime DE69530043T2 (de) | 1994-11-08 | 1995-11-07 | Verfahren zur Bestimmung einer fluoreszierenden Substanz, sowie Verfahren zur Enzym-Aktivität-Bestimmung |
Country Status (3)
Country | Link |
---|---|
US (1) | US5792662A (de) |
EP (1) | EP0711992B1 (de) |
DE (1) | DE69530043T2 (de) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10163775A1 (de) * | 2001-12-22 | 2003-07-03 | Roche Diagnostics Gmbh | Analysensystem zur Bestimmung einer Analytkonzentration unter Berücksichtigung von proben- und analytunabhängigen Lichtintensitätsänderungen |
ATE516504T1 (de) | 2008-02-07 | 2011-07-15 | Synapse Bv | Um den einfluss von störfaktoren korrigierte messung des zeitlichen verlaufs einer enzymatischen aktivität bei der reaktion eines enzyms mit einem substrat |
CN104204778A (zh) * | 2012-03-12 | 2014-12-10 | 三菱丽阳株式会社 | 荧光检测装置及荧光检测方法 |
CN104034705B (zh) * | 2013-03-06 | 2017-05-10 | 常州欣宏科生物化学有限公司 | 一种荧光探针检测酶活性方法 |
CN104677905B (zh) * | 2015-03-09 | 2017-03-08 | 大连工业大学 | 一种通过检测反应液中氨基酸浓度确定酶促反应底物浓度的方法 |
CN113720820A (zh) * | 2021-09-03 | 2021-11-30 | 东北师范大学 | β-葡萄糖苷酶活性检测用免标记型光学纳米传感器及应用 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4954435A (en) * | 1987-01-12 | 1990-09-04 | Becton, Dickinson And Company | Indirect colorimetric detection of an analyte in a sample using ratio of light signals |
US4788138A (en) * | 1987-04-30 | 1988-11-29 | Beckman Instruments, Inc. | Method to achieve a linear standard curve in a sandwich immunoassay |
US4990784A (en) * | 1989-08-25 | 1991-02-05 | Process Automation Business, Inc. | Nonlinear averaging compensated measurements |
FR2672128B1 (fr) * | 1991-01-28 | 1995-08-18 | Cis Bio Int | Procede de mesure de la luminescence emise dans un dosage par luminescence. |
JP2912957B2 (ja) * | 1991-06-18 | 1999-06-28 | 東ソー株式会社 | 酵素活性測定方法及び装置 |
-
1995
- 1995-11-07 EP EP95117517A patent/EP0711992B1/de not_active Expired - Lifetime
- 1995-11-07 DE DE69530043T patent/DE69530043T2/de not_active Expired - Lifetime
-
1997
- 1997-04-24 US US08/842,405 patent/US5792662A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP0711992A2 (de) | 1996-05-15 |
DE69530043D1 (de) | 2003-04-30 |
US5792662A (en) | 1998-08-11 |
EP0711992B1 (de) | 2003-03-26 |
EP0711992A3 (de) | 1997-04-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |