DE69530043D1 - Verfahren zur Bestimmung einer fluoreszierenden Substanz, sowie Verfahren zur Enzym-Aktivität-Bestimmung - Google Patents

Verfahren zur Bestimmung einer fluoreszierenden Substanz, sowie Verfahren zur Enzym-Aktivität-Bestimmung

Info

Publication number
DE69530043D1
DE69530043D1 DE69530043T DE69530043T DE69530043D1 DE 69530043 D1 DE69530043 D1 DE 69530043D1 DE 69530043 T DE69530043 T DE 69530043T DE 69530043 T DE69530043 T DE 69530043T DE 69530043 D1 DE69530043 D1 DE 69530043D1
Authority
DE
Germany
Prior art keywords
determining
enzyme activity
fluorescent substance
determining enzyme
fluorescent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69530043T
Other languages
English (en)
Other versions
DE69530043T2 (de
Inventor
Hidechika Hayashi
Kazuya Kamata
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tosoh Corp
Original Assignee
Tosoh Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tosoh Corp filed Critical Tosoh Corp
Application granted granted Critical
Publication of DE69530043D1 publication Critical patent/DE69530043D1/de
Publication of DE69530043T2 publication Critical patent/DE69530043T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G01N21/276—Calibration, base line adjustment, drift correction with alternation of sample and standard in optical path
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
DE69530043T 1994-11-08 1995-11-07 Verfahren zur Bestimmung einer fluoreszierenden Substanz, sowie Verfahren zur Enzym-Aktivität-Bestimmung Expired - Lifetime DE69530043T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP27374694 1994-11-08

Publications (2)

Publication Number Publication Date
DE69530043D1 true DE69530043D1 (de) 2003-04-30
DE69530043T2 DE69530043T2 (de) 2003-10-16

Family

ID=17532001

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69530043T Expired - Lifetime DE69530043T2 (de) 1994-11-08 1995-11-07 Verfahren zur Bestimmung einer fluoreszierenden Substanz, sowie Verfahren zur Enzym-Aktivität-Bestimmung

Country Status (3)

Country Link
US (1) US5792662A (de)
EP (1) EP0711992B1 (de)
DE (1) DE69530043T2 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10163775A1 (de) * 2001-12-22 2003-07-03 Roche Diagnostics Gmbh Analysensystem zur Bestimmung einer Analytkonzentration unter Berücksichtigung von proben- und analytunabhängigen Lichtintensitätsänderungen
EP2088435B1 (de) 2008-02-07 2011-07-13 Synapse B.V. Um den Einfluss von Störfaktoren korrigierte Messung des zeitlichen Verlaufs einer enzymatischen Aktivität bei der Reaktion eines Enzyms mit einem Substrat
CN104204778A (zh) * 2012-03-12 2014-12-10 三菱丽阳株式会社 荧光检测装置及荧光检测方法
CN104034705B (zh) * 2013-03-06 2017-05-10 常州欣宏科生物化学有限公司 一种荧光探针检测酶活性方法
CN104677905B (zh) * 2015-03-09 2017-03-08 大连工业大学 一种通过检测反应液中氨基酸浓度确定酶促反应底物浓度的方法
CN113720820A (zh) * 2021-09-03 2021-11-30 东北师范大学 β-葡萄糖苷酶活性检测用免标记型光学纳米传感器及应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4954435A (en) * 1987-01-12 1990-09-04 Becton, Dickinson And Company Indirect colorimetric detection of an analyte in a sample using ratio of light signals
US4788138A (en) * 1987-04-30 1988-11-29 Beckman Instruments, Inc. Method to achieve a linear standard curve in a sandwich immunoassay
US4990784A (en) * 1989-08-25 1991-02-05 Process Automation Business, Inc. Nonlinear averaging compensated measurements
FR2672128B1 (fr) * 1991-01-28 1995-08-18 Cis Bio Int Procede de mesure de la luminescence emise dans un dosage par luminescence.
JP2912957B2 (ja) 1991-06-18 1999-06-28 東ソー株式会社 酵素活性測定方法及び装置

Also Published As

Publication number Publication date
DE69530043T2 (de) 2003-10-16
EP0711992B1 (de) 2003-03-26
EP0711992A3 (de) 1997-04-16
US5792662A (en) 1998-08-11
EP0711992A2 (de) 1996-05-15

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