ES2001417A6 - Procedimiento para la determinacion especifica de hdl- colesterol en el suero. - Google Patents
Procedimiento para la determinacion especifica de hdl- colesterol en el suero.Info
- Publication number
- ES2001417A6 ES2001417A6 ES8601650A ES8601650A ES2001417A6 ES 2001417 A6 ES2001417 A6 ES 2001417A6 ES 8601650 A ES8601650 A ES 8601650A ES 8601650 A ES8601650 A ES 8601650A ES 2001417 A6 ES2001417 A6 ES 2001417A6
- Authority
- ES
- Spain
- Prior art keywords
- cholesterol
- measurement
- bile acid
- serum
- hdl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 210000002966 serum Anatomy 0.000 title abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 108010023302 HDL Cholesterol Proteins 0.000 title 1
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 abstract 8
- HSINOMROUCMIEA-FGVHQWLLSA-N (2s,4r)-4-[(3r,5s,6r,7r,8s,9s,10s,13r,14s,17r)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methylpentanoic acid Chemical compound C([C@@]12C)C[C@@H](O)C[C@H]1[C@@H](CC)[C@@H](O)[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@H](C)C(O)=O)CC[C@H]21 HSINOMROUCMIEA-FGVHQWLLSA-N 0.000 abstract 4
- 108010089254 Cholesterol oxidase Proteins 0.000 abstract 4
- 235000012000 cholesterol Nutrition 0.000 abstract 4
- 238000005259 measurement Methods 0.000 abstract 4
- 108010055297 Sterol Esterase Proteins 0.000 abstract 3
- 102000000019 Sterol Esterase Human genes 0.000 abstract 3
- CTTJWXVQRJUJQW-UHFFFAOYSA-N 2,2-dioctyl-3-sulfobutanedioic acid Chemical compound CCCCCCCCC(C(O)=O)(C(C(O)=O)S(O)(=O)=O)CCCCCCCC CTTJWXVQRJUJQW-UHFFFAOYSA-N 0.000 abstract 2
- 229920003171 Poly (ethylene oxide) Polymers 0.000 abstract 2
- 150000001335 aliphatic alkanes Chemical group 0.000 abstract 2
- JXLHNMVSKXFWAO-UHFFFAOYSA-N azane;7-fluoro-2,1,3-benzoxadiazole-4-sulfonic acid Chemical compound N.OS(=O)(=O)C1=CC=C(F)C2=NON=C12 JXLHNMVSKXFWAO-UHFFFAOYSA-N 0.000 abstract 2
- 239000003613 bile acid Substances 0.000 abstract 2
- 239000003599 detergent Substances 0.000 abstract 2
- 150000003839 salts Chemical class 0.000 abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 1
- 239000012736 aqueous medium Substances 0.000 abstract 1
- 239000012928 buffer substance Substances 0.000 abstract 1
- 125000004432 carbon atom Chemical group C* 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 238000006395 oxidase reaction Methods 0.000 abstract 1
- 230000036284 oxygen consumption Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Endocrinology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
PROCEDIMIENTO PARA LA DETERMINACION ESPECIFICA DE HDL-COLESTEROL EN EL SUERO. SE DESCRIBE UN PROCEDIMIENTO PARA LA DETERMINACION ESPECIFICA DE HDL-COLESTEROL EN EL SUERO O EN EL PLASMA, POR INCUBACION CON UN SISTEMA DE DETECCION DE COLESTEROL, QUE CONTIENE COLESTEROL-OXIDASA Y COLESTEROL-ESTERASA EN UN MEDIO ACUOSO TAMPONADO Y MEDICION DE UN PRODUCTO DE LA REACCION DE COLESTEROL-OXIDASA O DEL CONSUMO DE OXIGENO, EL CUAL REACTIVO O PROCEDIMIENTO ESTA CARACTERIZADO PORQUE SE INCUBA EN PRESENCIA DE UNA SAL DE UN ACIDO BILIAR O DE UNA SAL DE UN DERIVADO DE UN ACIDO BILIAR O DE DIOCTILSULFOSUCCINATO, LUEGO SE LLEVA A CABO UNA PRIMERA MEDICION, A CONTINUACION SE AÑADE UN DETERGENTE NO IONICO, QUE CONTIENE GRUPOS POLI(OXIDO DE ETILENO) O UN ALCANSULFONATO SECUNDARIO, SE INCUBA DE NUEVO Y LUEGO SE LLEVA A CABO UNA SEGUNDA MEDICION, Y LA CANTIDAD DE HDL-COLESTEROL SE DETERMINA A PARTIR DE LA DIFERENCIA ENTRE LA PRIMERA MEDICION Y LA SEGUNDA MEDICION.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19853533288 DE3533288A1 (de) | 1985-09-18 | 1985-09-18 | Verfahren und reagenz zur spezifischen bestimmung von hdl-cholesterin im serum |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2001417A6 true ES2001417A6 (es) | 1988-05-16 |
Family
ID=6281297
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES8601650A Expired ES2001417A6 (es) | 1985-09-18 | 1986-09-05 | Procedimiento para la determinacion especifica de hdl- colesterol en el suero. |
Country Status (10)
Country | Link |
---|---|
US (1) | US4851335A (es) |
EP (1) | EP0218127B1 (es) |
JP (1) | JPH0616720B2 (es) |
KR (1) | KR890003948B1 (es) |
AT (1) | ATE48649T1 (es) |
AU (1) | AU574931B2 (es) |
DE (2) | DE3533288A1 (es) |
DK (1) | DK445986A (es) |
ES (1) | ES2001417A6 (es) |
FI (1) | FI83975C (es) |
Families Citing this family (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE448324B (sv) * | 1985-09-27 | 1987-02-09 | Pharmacia Ab | Sett vid immunkemisk bestemning av lipoproteiner och apolipoproteiner |
US5221623A (en) * | 1986-07-22 | 1993-06-22 | Boyce Thompson Institute For Plant Research, Inc. | Use of bacterial luciferase structural genes for cloning and monitoring gene expression in microorganisms and for tagging and identification of genetically engineered organisms |
DE3636851A1 (de) * | 1986-10-29 | 1988-05-11 | Boehringer Mannheim Gmbh | Verfahren und reagenz zur spezifischen bestimmung des cholesterins der hdl-fraktion |
EP0378395B1 (en) * | 1989-01-13 | 1996-08-14 | Robert Q. Maines | Method of measuring cholesterol, diagnosing vascular disease, and raising HDL cholesterol levels |
US5135716A (en) * | 1989-07-12 | 1992-08-04 | Kingston Diagnostics, L.P. | Direct measurement of HDL cholesterol via dry chemistry strips |
FI901703A (fi) * | 1990-04-04 | 1991-10-05 | Elomit Oy | Foerfarande foer bestaemmande av kliniskt relevanta metaboliter. |
US5344571A (en) * | 1992-02-18 | 1994-09-06 | University Of Texas System Board Of Regents | Method for separating isoanalytes and measuring analytes in fluids |
JP2653755B2 (ja) * | 1994-03-08 | 1997-09-17 | 協和メデックス株式会社 | 高密度リポ蛋白中のコレステロールの定量法 |
JP2600065B2 (ja) * | 1994-03-08 | 1997-04-16 | 協和メデックス株式会社 | 高密度リポ蛋白中のコレステロールの定量法 |
EP0764848A4 (en) * | 1995-03-20 | 2001-04-11 | Kyowa Medex Co Ltd | METHOD FOR QUANTIFYING CHOLESTEROL IN LOW DENSITY OR VERY LOW DENSITY LIPOPROTEIN |
US5776719A (en) * | 1997-07-07 | 1998-07-07 | Mercury Diagnostics, Inc. | Diagnostic compositions and devices utilizing same |
US5989845A (en) * | 1996-04-05 | 1999-11-23 | Mercury Diagnostics, Inc. | Diagnostic compositions and devices utilizing same |
US6040151A (en) | 1998-03-10 | 2000-03-21 | Mercury Diagnostics, Inc. | Diagnostic compositions and devices utilizing same |
WO1997040376A1 (fr) * | 1996-04-22 | 1997-10-30 | Iatron Laboratories, Inc. | Procede specifique de dosage du cholesterol des lphd et composition utilisee pour le dosage. |
JP3193634B2 (ja) | 1996-05-29 | 2001-07-30 | 第一化学薬品株式会社 | Ldlコレステロールの定量方法 |
CA2246308C (en) * | 1996-12-09 | 2008-11-18 | Denka Seiken Co., Ltd. | Method for quantifying cholesterol in high-density lipoprotein |
JPH1156395A (ja) * | 1997-08-27 | 1999-03-02 | Dai Ichi Pure Chem Co Ltd | コレステロールの定量法 |
AU2003301324A1 (en) * | 2002-10-16 | 2004-05-04 | Kyowa Medex Co., Ltd. | Method and reagent for measuring cholesterol in high-density lipoproteins |
JP4498143B2 (ja) * | 2002-10-16 | 2010-07-07 | 協和メデックス株式会社 | 高密度リポ蛋白中のコレステロールの測定方法および試薬 |
TWI372783B (en) * | 2005-04-27 | 2012-09-21 | Kyowa Medex Co Ltd | A process for measuring the cholesterol in high density lipoprotein |
CN100365409C (zh) * | 2005-06-01 | 2008-01-30 | 王贤理 | 一种测定低密度脂蛋白胆固醇的试剂及制备方法 |
GB0609494D0 (en) * | 2006-05-12 | 2006-06-21 | Oxford Biosensors Ltd | HDL cholesterol sensor using specific surfactant |
GB0609493D0 (en) * | 2006-05-12 | 2006-06-21 | Oxford Biosensors Ltd | Cholesterol sensor |
WO2008087895A1 (ja) * | 2007-01-15 | 2008-07-24 | Toyo Boseki Kabushiki Kaisha | 高密度リポ蛋白中コレステロール測定方法およびその試薬 |
JP2014030393A (ja) * | 2012-08-03 | 2014-02-20 | Denka Seiken Co Ltd | 高密度リポ蛋白(hdl)中のコレステロールの定量方法 |
US9670525B2 (en) | 2012-08-31 | 2017-06-06 | Kyowa Medex Co., Ltd. | Method for measuring cholesterol in high-density lipoprotein |
CN115247161B (zh) * | 2021-09-14 | 2024-06-25 | 武汉瀚海新酶生物科技有限公司 | Genapol X-080用于提高胆固醇酯酶活性的应用和方法 |
CN114216871B (zh) * | 2021-12-15 | 2024-02-02 | 青岛汉唐生物科技有限公司 | 用于胆固醇试剂盒中替代胆酸钠的复合稳定剂 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4164448A (en) * | 1973-12-07 | 1979-08-14 | Boehringer Mannheim Gmbh | Activation of cholesterol oxidase for cholesterol assay |
US4184921A (en) * | 1976-03-25 | 1980-01-22 | Boehringer Mannheim Gmbh | Process and reagent for determining cholesterol |
US4275152A (en) * | 1977-02-03 | 1981-06-23 | Eastman Kodak Company | Hydrolysis of protein-bound cholesterol esters |
US4205521A (en) * | 1977-03-21 | 1980-06-03 | Victoria Torres | Decorative clock |
US4105521A (en) * | 1977-09-21 | 1978-08-08 | Helena Laboratories Corporation | Clinical procedure for measuring lipoprotein cholesterols |
US4226713A (en) * | 1978-04-24 | 1980-10-07 | Goldberg Jack M | Diagnostic agents |
US4414326A (en) * | 1978-04-24 | 1983-11-08 | Goldberg Jack M | Diagnostic agents |
EP0006822B1 (fr) * | 1978-07-04 | 1982-10-13 | Laboratoires Goella | Procédé et ensemble de réactifs pour le dosage du cholestérol sérique |
DE3276390D1 (en) * | 1981-09-10 | 1987-06-25 | Intermedicat Gmbh | Method for the selective extracorporeal precipitation of low-density lipoproteins from serum or plasma |
FR2513766B1 (fr) * | 1981-09-28 | 1985-09-13 | Biomerieux Sa | Procedes et reactifs de separation selective des lipoproteines de faible densite ldl et de quantification de leurs composants |
DE3208253A1 (de) * | 1982-03-08 | 1983-09-15 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur spezifischen bestimmung des cholesterins der ldl-fraktion im serum |
DE3369792D1 (en) * | 1982-04-02 | 1987-03-19 | Ivan Endre Modrovich | Improved time-stable liquid cholesterol assay compositions |
-
1985
- 1985-09-18 DE DE19853533288 patent/DE3533288A1/de not_active Withdrawn
-
1986
- 1986-08-14 AU AU61163/86A patent/AU574931B2/en not_active Ceased
- 1986-09-05 ES ES8601650A patent/ES2001417A6/es not_active Expired
- 1986-09-16 US US06/908,031 patent/US4851335A/en not_active Expired - Lifetime
- 1986-09-17 FI FI863752A patent/FI83975C/fi not_active IP Right Cessation
- 1986-09-17 DK DK445986A patent/DK445986A/da unknown
- 1986-09-17 KR KR1019860007833A patent/KR890003948B1/ko not_active IP Right Cessation
- 1986-09-18 AT AT86112875T patent/ATE48649T1/de not_active IP Right Cessation
- 1986-09-18 DE DE8686112875T patent/DE3667492D1/de not_active Expired - Lifetime
- 1986-09-18 EP EP86112875A patent/EP0218127B1/de not_active Expired
- 1986-09-18 JP JP61218274A patent/JPH0616720B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DK445986A (da) | 1987-03-19 |
FI863752A (fi) | 1987-03-19 |
FI83975B (fi) | 1991-06-14 |
KR870003205A (ko) | 1987-04-15 |
FI863752A0 (fi) | 1986-09-17 |
AU6116386A (en) | 1987-03-19 |
DE3667492D1 (de) | 1990-01-18 |
DE3533288A1 (de) | 1987-03-26 |
JPH0616720B2 (ja) | 1994-03-09 |
EP0218127A1 (de) | 1987-04-15 |
JPS6269999A (ja) | 1987-03-31 |
ATE48649T1 (de) | 1989-12-15 |
DK445986D0 (da) | 1986-09-17 |
EP0218127B1 (de) | 1989-12-13 |
US4851335A (en) | 1989-07-25 |
KR890003948B1 (ko) | 1989-10-13 |
FI83975C (fi) | 1991-09-25 |
AU574931B2 (en) | 1988-07-14 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 20100623 |