EP4281067A1 - Verfahren zur erhöhung der antioxidativen wirkung von vitamin c in wässriger lösung - Google Patents
Verfahren zur erhöhung der antioxidativen wirkung von vitamin c in wässriger lösungInfo
- Publication number
- EP4281067A1 EP4281067A1 EP22704931.9A EP22704931A EP4281067A1 EP 4281067 A1 EP4281067 A1 EP 4281067A1 EP 22704931 A EP22704931 A EP 22704931A EP 4281067 A1 EP4281067 A1 EP 4281067A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- vitamin
- composition
- overactivated
- solution
- treatment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 title claims abstract description 125
- 238000000034 method Methods 0.000 title claims abstract description 42
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 31
- 239000007864 aqueous solution Substances 0.000 title claims abstract description 16
- 229960005070 ascorbic acid Drugs 0.000 title 1
- 230000002708 enhancing effect Effects 0.000 title 1
- 239000000203 mixture Substances 0.000 claims abstract description 65
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 61
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 61
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 61
- 239000011718 vitamin C Substances 0.000 claims abstract description 61
- 238000002360 preparation method Methods 0.000 claims abstract description 40
- 150000003700 vitamin C derivatives Chemical class 0.000 claims abstract description 29
- 239000002537 cosmetic Substances 0.000 claims abstract description 11
- 239000002417 nutraceutical Substances 0.000 claims abstract description 11
- 235000021436 nutraceutical agent Nutrition 0.000 claims abstract description 11
- 239000000243 solution Substances 0.000 claims description 25
- 230000008569 process Effects 0.000 claims description 19
- 238000011282 treatment Methods 0.000 claims description 19
- 230000000694 effects Effects 0.000 claims description 17
- 238000002604 ultrasonography Methods 0.000 claims description 15
- 239000000839 emulsion Substances 0.000 claims description 14
- 239000004615 ingredient Substances 0.000 claims description 11
- 239000012736 aqueous medium Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 229930003231 vitamin Natural products 0.000 claims description 8
- 235000013343 vitamin Nutrition 0.000 claims description 8
- 239000011782 vitamin Substances 0.000 claims description 8
- 229940088594 vitamin Drugs 0.000 claims description 8
- 230000015572 biosynthetic process Effects 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 4
- 102000008186 Collagen Human genes 0.000 claims description 3
- 108010035532 Collagen Proteins 0.000 claims description 3
- 206010028980 Neoplasm Diseases 0.000 claims description 3
- 229920001436 collagen Polymers 0.000 claims description 3
- 239000003086 colorant Substances 0.000 claims description 3
- 239000007854 depigmenting agent Substances 0.000 claims description 3
- 238000004090 dissolution Methods 0.000 claims description 3
- 201000001441 melanoma Diseases 0.000 claims description 3
- 230000003020 moisturizing effect Effects 0.000 claims description 3
- 239000003755 preservative agent Substances 0.000 claims description 3
- 208000024827 Alzheimer disease Diseases 0.000 claims description 2
- 206010003591 Ataxia Diseases 0.000 claims description 2
- 208000014644 Brain disease Diseases 0.000 claims description 2
- 208000002177 Cataract Diseases 0.000 claims description 2
- 206010061216 Infarction Diseases 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 claims description 2
- 206010012601 diabetes mellitus Diseases 0.000 claims description 2
- 230000002526 effect on cardiovascular system Effects 0.000 claims description 2
- 230000035876 healing Effects 0.000 claims description 2
- 210000000987 immune system Anatomy 0.000 claims description 2
- 230000007574 infarction Effects 0.000 claims description 2
- 229910052742 iron Inorganic materials 0.000 claims description 2
- 201000010901 lateral sclerosis Diseases 0.000 claims description 2
- 208000002780 macular degeneration Diseases 0.000 claims description 2
- 208000030159 metabolic disease Diseases 0.000 claims description 2
- 208000005264 motor neuron disease Diseases 0.000 claims description 2
- 230000004770 neurodegeneration Effects 0.000 claims description 2
- 208000015122 neurodegenerative disease Diseases 0.000 claims description 2
- 230000008397 ocular pathology Effects 0.000 claims description 2
- 239000003921 oil Substances 0.000 claims description 2
- 235000019198 oils Nutrition 0.000 claims description 2
- 230000007170 pathology Effects 0.000 claims description 2
- 239000013589 supplement Substances 0.000 claims description 2
- 235000015112 vegetable and seed oil Nutrition 0.000 claims description 2
- 239000008158 vegetable oil Substances 0.000 claims description 2
- 239000000341 volatile oil Substances 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims 1
- 235000006708 antioxidants Nutrition 0.000 abstract description 12
- 239000003963 antioxidant agent Substances 0.000 abstract description 11
- -1 agri-food Substances 0.000 abstract 1
- 238000009472 formulation Methods 0.000 description 9
- 239000007788 liquid Substances 0.000 description 6
- 239000008194 pharmaceutical composition Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 235000001014 amino acid Nutrition 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 230000036542 oxidative stress Effects 0.000 description 4
- 238000009210 therapy by ultrasound Methods 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000003708 ampul Substances 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- 239000011573 trace mineral Substances 0.000 description 3
- 235000013619 trace mineral Nutrition 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 230000000699 topical effect Effects 0.000 description 2
- 244000144725 Amygdalus communis Species 0.000 description 1
- 235000011437 Amygdalus communis Nutrition 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 239000010478 argan oil Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003240 coconut oil Substances 0.000 description 1
- 235000019864 coconut oil Nutrition 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000008406 cosmetic ingredient Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 210000001198 duodenum Anatomy 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 230000035612 epigenetic expression Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008131 herbal destillate Substances 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 150000002433 hydrophilic molecules Chemical class 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 210000001630 jejunum Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000007764 o/w emulsion Substances 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 231100000628 reference dose Toxicity 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 235000010378 sodium ascorbate Nutrition 0.000 description 1
- PPASLZSBLFJQEF-RKJRWTFHSA-M sodium ascorbate Substances [Na+].OC[C@@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RKJRWTFHSA-M 0.000 description 1
- 229960005055 sodium ascorbate Drugs 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- PPASLZSBLFJQEF-RXSVEWSESA-M sodium-L-ascorbate Chemical compound [Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RXSVEWSESA-M 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 239000000230 xanthan gum Substances 0.000 description 1
- 229940082509 xanthan gum Drugs 0.000 description 1
- 235000010493 xanthan gum Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/365—Lactones
- A61K31/375—Ascorbic acid, i.e. vitamin C; Salts thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/676—Ascorbic acid, i.e. vitamin C
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
Definitions
- the present invention relates to the field of antioxidant compositions comprising vitamin C. More particularly, it relates to a process for improving the activity of vitamin C in aqueous solution, as well as various compositions comprising superactivated vitamin C and their preparation process. These compositions can be used in particular in the cosmetic, nutraceutical, pharmaceutical or food-processing fields.
- Vitamin C is a molecule known and used for a long time for its antioxidant properties, in particular for its ability to inhibit free radicals, and therefore oxidative stress. It is more generally found in dry form, pure or mixed with other compounds. In recent years, the dry liposomal form (vitamin C coated with liposome) has been particularly developed to improve its assimilation by the body. Assimilation occurs mainly at the level of the duodenum and the proximal jejunum and is saturable, the percentage absorbed decreasing even with the dose ingested. It is also sodium-dependent and can be inhibited by structural analogs.
- vitamin C has also been examined at the cutaneous level. In addition to its antioxidant role, its presence in high concentrations in the skin could be associated with other functions, such as the formation of collagen, the inhibition of the formation of melanomas or even the modulation of epigenetic expressions.
- vitamin C The bioavailability of vitamin C varies greatly depending on the method of preparation and assimilation. Thus, depending on the form (dry, liposomal, liquid, emulsified, etc.) and the preparation technique used, the same amount of vitamin C absorbed can lead to:
- Dry forms are widely preferred today, to guard against possible problems of chemical stability (degradation of vitamin C over time), physical (phase separation of emulsions for example) or microbiological encountered with aqueous solutions.
- chemical stability degradation of vitamin C over time
- physical phase separation of emulsions for example
- microbiological encountered with aqueous solutions it is well known that the assimilation and bioavailability of vitamin C in dry form are not optimal.
- New products are regularly offered on the market to stand out from the competition with a formulation likely to make vitamin C more active in the broad sense. It is known to use a treatment for the preparation of stable emulsions comprising a lipid phase in an aqueous phase using piezoelectric transducers.
- patent WO2010/149668 describes a method for preparing an oil-in-water emulsion produced in a container in which is immersed a piezoelectric transducer operating at high frequencies, in particular greater than 900 kHz. It has also been proposed in patent WO2017/037372 to use a similar process, using ultrasound at comparable frequencies, to vectorize hydrophobic active principles, in the form of a dispersed phase in a hydrophilic continuous phase. It has in particular been proposed to increase the bioavailability of co-enzyme Q.10 by this process.
- the invention relates to a process for improving the antioxidant activity in solution in an aqueous medium consisting in treating said solution with ultrasound at a vibration frequency greater than or equal to 1 MHz.
- the invention also relates to a process for the preparation of an aqueous antioxidant composition comprising overactivated vitamin C, said composition being treated by application of the process of the invention, as well as the composition obtained.
- the invention also relates to a process for preparing an emulsion comprising overactivated vitamin C, as well as the composition obtained in the form of an emulsion.
- composition according to the invention in the cosmetic, nutraceutical and pharmaceutical field.
- the main advantage of the process according to the invention is that it makes it possible to increase the antioxidant activity of vitamin C in solution by at least 10%, preferably by at least 20%. This property is quite surprising and very interesting.
- the process is particularly interesting because it makes it possible to formulate vitamin C in aqueous solution, that is to say that it makes it possible to prepare a stable liquid aqueous composition of vitamin C without adding stabilizing compounds (without adding active chemical, without texturizing, without emulsifying or any other compound known to stabilize solutions). It paves the way for the preparation of new formulations of vitamin C in aqueous solution. Such formulations can be presented in the form of a glass ampoule or a plastic pod.
- the dose of vitamin C can be reduced for the same effectiveness with respect to the dose comprised in the compositions available to date.
- the antioxidant activity will be higher with an overactivated vitamin C as defined in the invention.
- compositions according to the invention give access to new antioxidant formulations which can be used in cosmetics, in nutraceuticals, in pharmaceuticals and in the food industry.
- a first object of the invention relates to a process for improving the activity of vitamin C in aqueous solution, consisting in treating said solution containing vitamin C by ultrasound at a vibration frequency greater than or equal to 1 MHz.
- the main activity of vitamin C is antioxidant activity.
- Antioxidants can be defined as "any substance which, in low concentration relative to a substrate liable to be oxidized, prevents or slows down the oxidation of this substrate".
- antioxidant activity within the meaning of the invention, is meant the action of preventing or slowing down the oxidation of a substrate. This activity can be measured in particular using the PAOT-Liquid® method, which records the modifications of an electrochemical potential in a reaction medium containing a reference molecule in its free radical state; this method is described in patent application WO2020/109736.
- aqueous solution within the meaning of the invention, is meant any type of solution composed mainly of water, including a solution of pure or quasi-pure water.
- aqueous solution is meant any type of solution composed mainly of water, including a solution of pure or quasi-pure water.
- the treatment time can vary and is dependent on the volume of preparation to be treated, the amount of vitamin C, and the type and power of the ultrasonic transmitters.
- the frequency of the ultrasounds is between 1 and 3 MHz and is applied, for volumes between lOmL and 1000L, for a period that can range from 15 min to 24 hours.
- the treatment time can also be adapted according to the volume treated in batch, or the flow rate for continuous treatments.
- This process makes it possible to formulate vitamin C, a hydrophilic molecule, in solution in an aqueous medium by process, without adding any chemical active ingredient. It is therefore a very simple formulation which may contain only vitamin C, the effect of improving antioxidant activity is obtained through ultrasonic treatment. This improvement can reach 10%, even 20% or more; the activity obtained is maintained for a period of at least 3 weeks without any special precautions (ambient temperature, without inerting).
- a second object of the invention relates to a process for the preparation of an aqueous composition comprising overactivated vitamin C comprising the steps of: dissolving the vitamin C in an aqueous medium treating said solution by ultrasound at a vibrating frequency greater than or equal to 1 MHz.
- overactivated vitamin C within the meaning of the invention, is meant a vitamin C whose antioxidant activity is increased by at least 10% compared to the same quantity of reference vitamin C (untreated, in particular not treated with ultrasound), preferably by at least 20%; the method for calculating this increase in activity is described in the experimental part.
- the superactivated vitamin C according to the invention is a vitamin C treated in solution by ultrasound at a vibrational frequency greater than or equal to 1 MHz.
- a third subject of the invention relates to an aqueous composition comprising superactivated vitamin C, characterized in that said superactivated vitamin C has an antioxidant activity that is at least 10% greater than that of the same quantity of reference vitamin C; it is obtained by treatment using the method defined above.
- This composition can be called “generic composition” in the remainder of this text.
- Formulation of the composition may be limited to the superactivated vitamin itself in water.
- the aqueous medium (or aqueous solution) in which the vitamin C is dissolved can contain other molecules, for example vitamins, amino acids, one or more texturizing molecules, etc.
- the generic composition can also be used as an ingredient for the preparation of complex compositions.
- a fourth object of the invention relates to a process for the preparation of an emulsion of the oil-in-water type comprising overactivated vitamin C comprising the steps of: dissolution of vitamin C in an aqueous medium addition of an oily solution in a proportion of between 1% and 30% relative to the quantity by mass of the aqueous solution treatment of said solution by ultrasound at a higher or equal vibration frequency at 1MHz.
- the oily solution used in the emulsion can be any type of oil or oily substance, alone or as a mixture, in particular vegetable oils, among which mention may be made, by way of example, of olive oil, sweet almond, argan oil, coconut oil... but also essential oils.
- a fifth object of the invention relates to an emulsion comprising overactivated vitamin C characterized in that it is obtained by ultrasound treatment at a vibrational frequency greater than or equal to 1 MHz and that said overactivated vitamin C has antioxidant activity at least 10% higher than that of the same reference quantity of vitamin C.
- the present invention provides access to the preparation of an ampoule or a pod containing an aqueous composition of superactivated vitamin C in stable form in aqueous solution as defined above.
- the ampoule and the pod are themselves embodiments of the invention.
- the invention can be applied to the implementation of a process for the preparation of a cosmetic composition comprising overactivated vitamin C comprising the steps of: dissolving the vitamin C in an aqueous medium optionally adding a solution oily in a proportion of between 1% and 30% relative to the mass quantity of the aqueous solution addition of at least one cosmetic ingredient chosen from: vitamins other than vitamin C, moisturizing agents, depigmenting agents, texturizers, pH correctors and coloring agents treatment of the said solution obtained by ultrasound at a vibration frequency greater than or equal to 1 MHz.
- the invention also relates to a cosmetic composition
- a cosmetic composition comprising overactivated vitamin C, characterized in that it is obtained by treatment using the method defined above and that it comprises, in addition to vitamin C, at least one ingredient chosen among vitamins other than vitamin C, moisturizing agents, depigmenting agents, texturizing agents, pH correctors and coloring agents.
- the cosmetic composition can also be used as an ingredient for the preparation of complex compositions.
- the cosmetic composition can also be formulated as a lotion, milk, cream or the like.
- a sixth object of the invention relates to the use of a composition as defined previously (generic composition or cosmetic composition) to aid in the regeneration of the skin and/or to stimulate the formation of collagen and/or to improve its radiance.
- the invention can be applied to the implementation of a process for the preparation of a nutraceutical composition
- a process for the preparation of a nutraceutical composition comprising overactivated vitamin C comprising the steps of dissolving the vitamin C in an aqueous medium optional addition of an oily solution in a proportion of between 1% and 30% relative to the quantity by mass of the aqueous solution, addition of at least one component chosen from vitamins other than vitamin C, trace elements and amino acids, treatment of said solution obtained by ultrasound at a vibration frequency greater than or equal to 1 MHz.
- the invention also relates to a nutraceutical composition
- a nutraceutical composition comprising overactivated vitamin C, characterized in that it is obtained by treatment using the method defined above and that it comprises, in addition to vitamin C, at least one component chosen among vitamins other than vitamin C, trace elements and amino acids.
- the nutraceutical composition can also be used as an ingredient for the preparation of complex compositions.
- a seventh object of the invention relates to the use of a composition as defined previously (generic composition or nutraceutical composition) in nutraceuticals to prevent the effects of oxidative stress; it allows in particular to stimulate the defenses of the body, in particular the immune system, to improve the absorption of iron, to fight against fatigue.
- a composition may include other vitamins, trace elements, amino acids.
- the invention can be applied to the implementation of a process for the preparation of a pharmaceutical composition comprising overactivated vitamin C comprising the steps of: dissolving the vitamin C in an aqueous medium adding at least one component chosen from active pharmaceutical principles, or excipients treatment of said solution obtained by ultrasound at a vibrational frequency greater than or equal to 1 MHz.
- the invention also relates to a pharmaceutical composition
- a pharmaceutical composition comprising overactivated vitamin C, characterized in that it is obtained by treatment using the method defined above and that it comprises, in addition to vitamin C, at least one component chosen among the active pharmaceutical principles, or the excipients.
- the pharmaceutical composition can also be used as an ingredient for the preparation of complex compositions.
- an eighth object of the invention relates to the use of a composition as defined above (generic composition or pharmaceutical composition) in pharmaceuticals as a medicament, in particular for preventing and/or treating diseases associated with oxidative stress.
- This pharmaceutical composition can be used for example to promote healing or prevent the formation of cancers, in particular melanomas.
- diseases associated with oxidative stress we mean cancers, ocular pathologies (cataract and macular degeneration), cardiovascular pathologies (infarction), neurodegenerative diseases (ataxia, lateral sclerosis, Alzheimer's disease), metabolic diseases ( diabetes), brain disease.
- the antioxidant compositions according to the invention can be used in drinkable, topical, ocular or injectable preparations. They can in particular be formulated from vitamin C only or in very short formulas to avoid the addition of compounds likely to induce side effects (allergic reactions, irritations, etc.).
- a ninth object of the invention relates to the use of a composition comprising overactivated vitamin C in the agrifood field as a supplement or replacement for preservatives.
- composition in the field of the food industry, a composition (generic composition) according to the invention can be added to drinks, applied to food or any other use in addition to or replacement of preservative products, for their antioxidant properties.
- a composition generic composition
- the present invention will be better understood on reading the examples which follow, provided by way of illustration and should in no way be considered as limiting the scope of the present invention.
- Table 1 Composition of the four preparations made. The values indicated are the mass percentages of the ingredients in the corresponding preparation.
- Preparations 1 and 2 were made from the same stock preparation of 2L, then separated into two. Preparations 1 and 3 were made by simply dispersing/mixing the ingredients all together. Preparations 2 and 4 underwent complementary high-frequency ultrasound treatment (USHF treatment), at 1.7 MHz. For preparation 4, the USHF treatment induced a significant drop in pH, which was compensated by an addition of 1% sodium hydroxide sufficient for the final pH of the preparation to be comparable to that of the other preparations, or by a value of about 5. This pH was chosen so as to be able to correspond to that of a potential product for topical use. For To reach it, vitamin C was provided in its conjugate acid and base forms. Xanthan gum was added to preparation 4 so that its viscosity was similar to that obtained for preparation 3 with the surfactants (Twin and Span).
- USHF treatment complementary high-frequency ultrasound treatment
- Table 2 PAOT score and standard deviations (SD) obtained for the four preparations made.
- the antioxidant potential of the active ingredient is the same when it is treated alone or when it is treated within an emulsion.
- the lowest score is obtained for the conventional emulsion, produced using surfactants, which is quite significant of what is generally observed, that is to say that the antioxidants present in complex formulations lose part of their activity due to their interactions with the other ingredients.
- surfactants or other stabilizers present in conventional emulsion formulations can lead to a form of encapsulation of vitamin C, also reducing its activity.
- the PAOT-Liquid® method is also used to reproducibly quantify vitamin C in water or blood.
- the relationship is linear and a PAOT score of 100 corresponds to a concentration of approximately 1g/L.
- the PI sample respects this rule perfectly since the concentration of vitamin C (conjugate acid and base) is in fact about 6.3% in the preparation, deducting the contribution of Na + ions from the sodium ascorbate .
- the expected score is therefore approximately 630, for a measured score of 665.67.
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Birds (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Cosmetics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR2100528A FR3118869A1 (fr) | 2021-01-20 | 2021-01-20 | Procede d’amelioration de l’activite antioxydante de la vitamine c en solution aqueuse |
PCT/FR2022/050114 WO2022157460A1 (fr) | 2021-01-20 | 2022-01-20 | Procede d'amelioration de l'activite antioxydante de la vitamine c en solution aqueuse |
Publications (1)
Publication Number | Publication Date |
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EP4281067A1 true EP4281067A1 (de) | 2023-11-29 |
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ID=76730594
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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EP22704931.9A Pending EP4281067A1 (de) | 2021-01-20 | 2022-01-20 | Verfahren zur erhöhung der antioxidativen wirkung von vitamin c in wässriger lösung |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP4281067A1 (de) |
FR (1) | FR3118869A1 (de) |
WO (1) | WO2022157460A1 (de) |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2947186B1 (fr) | 2009-06-24 | 2016-01-01 | Sas Genialis | Procede de preparation d'une emulsion huile-dans-eau stable |
FR3040312B1 (fr) | 2015-08-31 | 2019-06-07 | Genialis | Procede de traitement en continu d'un melange |
FR3072280A1 (fr) * | 2017-10-16 | 2019-04-19 | Isabelle Desjardins-Lavisse | Emulsion huile-dans-eau a base d’huiles essentielles et formulee sans tensio-actif |
FR3089011A1 (fr) | 2018-11-28 | 2020-05-29 | SMAIL Meziane | Procédé et dispositif de mesure du statut du stress oxydant dans une matrice biologique |
FR3098732B1 (fr) * | 2019-07-15 | 2022-08-12 | Genialis | Procede d’amelioration de la biodisponibilite de composes hydrophiles dans une solution aqueuse |
-
2021
- 2021-01-20 FR FR2100528A patent/FR3118869A1/fr active Pending
-
2022
- 2022-01-20 WO PCT/FR2022/050114 patent/WO2022157460A1/fr unknown
- 2022-01-20 EP EP22704931.9A patent/EP4281067A1/de active Pending
Also Published As
Publication number | Publication date |
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WO2022157460A1 (fr) | 2022-07-28 |
FR3118869A1 (fr) | 2022-07-22 |
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