EP4100164A1 - Pointe de pipette - Google Patents

Pointe de pipette

Info

Publication number
EP4100164A1
EP4100164A1 EP21714190.2A EP21714190A EP4100164A1 EP 4100164 A1 EP4100164 A1 EP 4100164A1 EP 21714190 A EP21714190 A EP 21714190A EP 4100164 A1 EP4100164 A1 EP 4100164A1
Authority
EP
European Patent Office
Prior art keywords
tubular body
pipette tip
attachment
flat
area
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21714190.2A
Other languages
German (de)
English (en)
Inventor
Hanna Rethwisch
Maren LEONHARDT
Laura Koch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Publication of EP4100164A1 publication Critical patent/EP4100164A1/fr
Pending legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/08—Ergonomic or safety aspects of handling devices
    • B01L2200/087—Ergonomic aspects
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric

Definitions

  • the invention relates to a pipette tip.
  • Pipette tips are used together with pipettes and other dosing devices in particular in medical, biological, biochemical and chemical laboratories for dosing liquids.
  • pipettes and other metering devices are referred to collectively as "pipetting devices”.
  • Pipette tips have an elongated, tubular body which at the lower end has a lower opening for the passage of liquid and at the upper end an upper opening for clamping onto the neck of a pipetting device.
  • Pipette tips usually have a generally conical shape, the cross section of which increases from the lower opening to the upper opening.
  • Standardized conical or frustoconical approaches with a standard geometry that is uniformly used by many manufacturers and which is characterized for each pipette tip size by a specific mean diameter and a specific cone angle of the conical approach are known.
  • Multi-channel pipetting devices are used to simultaneously take up liquid from one or more vessels or to dispense it into one or more vessels. They are often used for processing microtiter plates that have a large number of vessels in a matrix-like arrangement.
  • multichannel pipetting devices have a plurality of conical attachments which are arranged parallel to one another in one or more parallel rows and onto which the pipette tips can be clamped.
  • multichannel pipettes with 8, 12, 16 or 24 attachments in a row are known.
  • the pipetting device When designed as an air-cushion pipetting device, the pipetting device has at least one displacement device for air, which is connected in a communicating manner to a through hole of at least one attachment.
  • an air cushion By means of the displacement device, an air cushion can be displaced in order to suck in liquid into a pipette tip clamped onto the attachment and to expel it therefrom.
  • the Ver displacement device is usually designed as a cylinder with a piston displaceable therein.
  • displacement devices with a displacement chamber and at least one deformable wall are also known, with a deformation of the wall causing the displacement of the air cushion.
  • a small piston is arranged in the pipette tip, which is coupled to a coupling element of a piston drive of the Pipettiervor direction when the pipette tip is attached to an attachment, which can be displaced in a through hole of the attachment.
  • the liquid is absorbed into the pipette tip preferably in a single step or in several small steps.
  • the liquid is dispensed in a single step when pipetting and in several small steps when dispensing.
  • Pipetting devices usually have an ejector that acts on the upper edge of the pipette tip in order to push it away from the attachment.
  • the ejector can be pressed against the upper edges of several pipette tips at the same time.
  • the pipetting device can be a hand-held pipette which the user can hold and operate with only one hand. It can also be a dosing station ("pipetting station”) or an automatic dosing device (“automatic pipetting device”) in which a dosing head with one or more attachments on a robot arm or on another transmission system can be displaced above a work surface.
  • the pipetting device can also be part of a laboratory machine ("workstation”) which, in addition to dosing, can carry out other treatments of liquids (eg mixing, temperature control, analysis).
  • the pipette tip To avoid incorrect dosing, the pipette tip must be clamped tightly or sealingly onto the attachment. In addition, the forces for attaching and ejecting the pipette tip from the attachment must not be too high.
  • Conventional pipette tips are thick-walled and rigid in the contact area with the conical attachment. When plugging on, the pipette tips are elastically widened at the circumference by the attachment. The spring characteristic is steep, so that high slip-on forces have to be applied. After attaching, there is a correspondingly high static friction between the attachment and the pipette tip, which must be overcome when it is ejected. The user is burdened by the high forces involved in attaching and ejecting the pipette tip. This can trigger diseases that are summarized under the term "cumulative trauma disorders" (CTD). As far as the attachment and ejection takes place by means of motorized drives, these must be correspondingly powerful and have a high power consumption.
  • CTD cumulative trauma disorders
  • US Pat. No. 6,197,259 describes a pipette tip which, by using relatively low axial push-on forces of six pounds (26.7 N), can be firmly pushed onto a tip of a pipette and by using relatively low ejecting forces of three pounds (13.3 N) is thrown from this.
  • the pipette tip has a conical upper end with an inner diameter at the upper end which is larger than the diameter of the attachment of the pipette onto which the pipette tip is to be attached.
  • the pipette tip has a hollow central section and an annular sealing area at the connection between the upper end and the central section.
  • the middle section has a side wall with a wall thickness between 0.2 and 0.5 mm on and next to the sealing area.
  • the annular sealing area has an inner diameter smaller than a value "x" and is designed to engage the lower end of a sealing zone of the lug to be expanded radially when the lug is inserted. This creates a liquid-tight seal between the sealing zone of the attachment and the sealing area of the pipette tip.
  • the pipette tip has lateral stabilizing means on the inside, next to the sealing area, which come into engagement with the outer surface of the attachment in order to stabilize the pipette tip on the attachment.
  • the lateral stabilizing means have at least three contacts spaced from one another in the circumferential direction and extending inward from the inner surface of the pipette tip.
  • the diametrical spacing of the contacts is such that they easily engage the lower end of the hub and allow the lower end to slide past without widening the sidewalls of the pipette tip on which the contacts are arranged.
  • the pipette tip is stretched in the sealing area and immediately next to it.
  • the contacts guide the pipette tip on the attachment the side wall of the pipette tip is deformed inward between the contacts and is not expanded, as a result of which the force that has to be applied to push in the attachment is kept low.
  • the attachment can be pressed deeper into the pipette tip as the push-on force increases. Correspondingly high Ejection forces must be applied to loosen the pipette tip from the attachment. Due to the inward protruding contacts, the construction is only suitable for relatively large pipette tips.
  • US Pat. No. 6,568,288 describes a pipette tip which has annular sealing and essentially cylindrical lateral guide areas which are axially spaced apart from one another, the sealing area being sufficiently thin to provide a press fit when a pipette attachment with axially spaced apart annular sealing and cylindrical lateral guide zones penetrates to form airtight seal between a sealing surface of the sealing zone and the sealing area.
  • the wall thickness in the sealing area is preferably between 0.2 and 0.5 mm.
  • the sealing surface is the outer surface of an annular, radially outwardly projecting projection next to the lower end of the extension.
  • the pipette tip has a circular, upward-facing and inward-facing shoulder on the inner circumference in order to limit the attachment to the attachment.
  • the pushing force should be approximately two pounds (8.9 N) and about one pound (4.45 N) for ejecting.
  • the depth stop can result in incomplete immersion when several pipette tips are picked up from a tray or rack at the same time using a multi-channel pipetting device. If the tray or rack bends slightly downwards between lateral edges, placing the two outer attachments on the shoulders of the two outer pipette tips can lead to insufficient immersion of the other attachments in the pipette tips arranged between them.
  • No. 6,967,004 B2 describes a pipette tip which has a circular ring-shaped sealing area with an inner sealing surface on a side wall that is sufficiently thin in the sealing area to expand slightly and create a To form a press fit and an airtight seal between the sealing surface and a sealing zone of the approach of the pipette inserted into the pipette tip.
  • the pipette tip has a circular, inwardly and upwardly directed shoulder that limits the insertion of the attachment.
  • the approach has two Zylin derabitese with different diameters.
  • Its annular sealing zone includes a sealing edge at a connection between the lower end of a cylinder section and the outermost edge of a radially extending transition of the extension.
  • the forces to insert and eject the pipette tip are less than two pounds (8.9 N). If several pipette tips are picked up at the same time by means of a multi-channel pipetting device, the depth stop can lead to insufficient immersion of approaches.
  • EP 2 138 234 A1 describes a pipette tip that has a flexible, tubular connecting section with a contour that is undulating in cross section, which increases the extensibility of the seat area, for releasable connection with the approach of a pipetting device at the upper end of an elongated tubular section.
  • the seating area is reversibly expandable by more than 20% when it is attached to the attachment.
  • the wave-shaped contour must be drawn smooth on the approach, so that the further elasticity is only low.
  • the pipette tip requires precise manufacture.
  • an inwardly radially protruding shoulder that causes a depth stop for the approach that can lead to insufficient immersion of approaches when picking up pipette tips by means of a multichannel pipetting device.
  • EP 2 606 977 A1 describes a pipette tip with the shape of an elongated tube with a lower opening at the lower end for the passage of liquid and an upper opening at the upper end, in addition to the upper Opening on the inner circumference there is a seat area which is used to attach to a standardized conical attachment of a pipetting device.
  • the Wegbe rich has a holding area with radially inwardly projecting, axially extending ribs and below the holding area a sealing area with a circumferential, inwardly projecting sealing projection.
  • the seat area is designed in such a way that the ribs are partially plastically deformed when plugged onto the attachment with a push-on force that ensures that the pipette tip is held and sealed on the attachment and an elastic deformation occurs outside the ribs in the seat area.
  • Below the sealing area it has a braking area that widens conically towards the upper opening to limit attachment. This ensures a secure seal on the attachment of a pipetting device and the ejection force to be applied for ejection is significantly reduced.
  • the design is particularly suitable for relatively large pipette tips with a nominal volume of 2.5, 5.0 and 10 mL. It is less suitable for smaller pipette tips due to the difficult production of the filigree ribs.
  • EP 3 115 110 A1 describes a pipette tip with a tubular body and a seat area for attaching to a conical extension of a pipetting device, which has a circumferential, inwardly protruding sealing projection on the inner circumference at a distance from the upper opening, and a circumferential sealing projection below the sealing projection braking area tapering downward more than the shoulder and above the sealing projection has a circumferential, inwardly protruding support projection.
  • the sealing protrusion can be clamped onto the neck in a sealing manner under elastic deformation, with the braking area lying further down on the neck and the supporting protrusion further above resting on the neck without pre-tensioning or being spaced from the neck by a circumferential gap.
  • the pipette tip seals well and is secure on the
  • the attachment of a pipetting device can be clamped on, can be ejected from the attachment with reduced expenditure of force and is also well suited for smaller pipette tip sizes.
  • the disadvantage is the still high expenditure of force when clamping onto the approach and when stripping from the approach.
  • WO 2011/091308 A2 describes a pipette tip which has an annular flange at the proximal end of a proximal section and axially directed ribs in the proximal section which are spaced apart from one another in the circumferential direction.
  • the flange is intended to increase the rigidity of the pipette tip and to make it easier to align the dispenser with the pipette tip.
  • the ribs are intended to limit the axial expandability of the pipette tip in the proximal area.
  • the push-on forces of the pipette tips with a filling volume of 200 m ⁇ and 1000 m ⁇ on five different pipettes are over 1000 g (10 N) and reach up to 2,000 g (20N).
  • An ergonomically optimized pipette tip is known from US Pat. No. 7,335,337 B1, which can be reliably fixed on a pipette in which the push-on forces and ejected forces are reduced.
  • the pipette tip has elastic expansion elements by means of which the axial push-on force and ejection force are reduced.
  • the elastic expansion elements are arranged in an upper section of the pipette tip above a sealing ring encircling the inner circumference. They are formed by outwardly curved areas of reduced wall thickness between cylindrical or conical segments of the pipette tip. When the attachment of a pipette is inserted into the upper opening of the pipette tip, the expansion elements are pulled flat and the segmented wall sections expand.
  • the pipette tip is guided and aligned on the attachment by ribs on the inside of the wall segments.
  • the push-on forces are still high because the pipette tip is in the area of the circumferential sealing ring has a large wall thickness and can only expand slightly, and rise sharply when the expansion elements are drawn flat.
  • WO 2018/213196 A1 describes a pipette tip with a proximal section with alternating, longitudinally directed grooves and panels that are designed to facilitate the expansion and compression of the wall when the pipette tip is attached to a correspondingly designed dispensing device and is thus sealed in Engagement is. These measures reduce the axial force for clamping a pipette tip onto a dispensing device and releasing a pipette tip from a dispensing device for liquid.
  • the grooves are stepped, V- or U-shaped.
  • a plurality of grooves and panels are arranged alternately on the circumference of the proximal portion.
  • the invention is based on the object of providing a pipette tip which can be clamped onto an attachment of a pipetting device in a sufficiently firm and sealing manner with reduced attachment force and ejection force, has more favorable manufacturing properties with improved dimensional accuracy and strength and is better suited for identifying different types of pipette tips .
  • the object is achieved by a pipette tip with the features of claim 1.
  • Advantageous embodiments of the pipette tip are specified in the subclaims.
  • the plastic pipette tip according to the invention comprises an elongated, tubular body with a lower opening at a lower end of the tubular body for the passage of liquid and an upper opening at an upper end of the tubular body for clamping onto a neck of a pipetting device, in addition to the upper Opening on the inner circumference of the tubular body, there is a seating area for the approach, characterized in that in addition to the upper opening on the outer circumference of the tubular body there is at least one flattened area extending in the axial direction, the wall thickness of the tubular body extends in a cross-section through the tubular body Body in each case starting from one of the two areas of the tubular body adjoining the flattening in the flattening towards its central area gradually reduced and the flattening in the cross-section through the tubular body (i) a straight profile or (ii) a we has a curved profile less than the areas adjoining the flat.
  • the pipette tip according to the invention has a smaller wall thickness in the area of the flattened area than at the two lateral edges of the flattened area and possibly in the areas of the tubular body adjoining the flattened area. This improves the deformability of the pipette tip when it is clamped onto an attachment of a pipetting device, so that reliable sealing of the pipette tip on the attachment can be achieved even with comparatively low push-on forces.
  • the pipette tip can be designed so that it when clamped with a certain force on a defined approach of a pipetting device is exclusively elastically deformed.
  • the push-on force increases proportionally to the deformation.
  • the elastic deformation can be completely restored after the pipette tip has been released from the attachment.
  • plastic deformation occurs.
  • the plastic deformation is an irreversible deformation that does not reverse itself after the pipette tip is detached from the attachment. In the case of plastic deformation, the push-on force no longer increases or only increases slightly with the deformation.
  • the elastic or plastic deformation takes place preferably in the area of the flattened areas, since the pipette tip has the smallest wall thickness in addition to the upper opening in the area of the flattened area. As a result, the effort required to clamp the pipette tip onto the attachment in a sealing manner can be kept low. In the case of plastic deformation, the push-on force can be limited to a specified limit value.
  • the plastic compound can better fill the cavity in the injection molding tool in the area of the flattened area than in the area of the grooves in the conventional pipette tips due to the lower pressure losses at the flattened area.
  • weld lines can also be reduced and more dimensionally stable and stronger pipette tips can be achieved.
  • the increased strength can prevent the pipette tips from tearing open at the points with the smallest wall thickness due to the push-on forces.
  • the flattened area can be used to identify the pipette tips.
  • information about the pipette tip and / or its manufacture are attached, for example about the pipette tip size, the material or the degree of purity of the pipette tip, the manufacturer, the brand and / or the production tool used for the manufacture.
  • the pipette tip size is the largest volume that can be dosed with the pipette tip.
  • Pipette tips that differ from one another by at least one of the aforementioned criteria are also referred to in this application as “pipette tips of different pipette tip types”.
  • the identification can be generated during injection molding in the form of raised or recessed letters, numbers, characters or symbols or can be printed on later. Furthermore, identification by the user comes into consideration, for example by printing, writing using a writing tool or sticking a label.
  • the flattening itself can be used as a distinguishing feature in order to distinguish different types of pipette tips from one another.
  • the flattened area can also serve as anti-roll protection in order to prevent a pipette tip placed on a work surface or other surface from rolling away.
  • the flattened area can have a straight profile and / or an outwardly (convex) and / or inwardly (concave) profile.
  • the profile of the flattening is largely or exclusively straight or largely or exclusively curved outward or largely or exclusively curved inward.
  • the radius of curvature is constant along the respective profile.
  • the radius of curvature varies along the respective profile or part thereof.
  • the profile of the flattened portion is rectilinear in sections and the same or in sections differently curved.
  • the profile of the flattened area is curved outward or straight on the two edges and curved outward or inward between them, so that it is approximately V-shaped overall.
  • the profile of the flattening is curved differently in sections.
  • the different curvatures can be curvatures with different radii of curvature or outward and inward curvatures with the same or different radii of curvature.
  • the curvature is less than the curvature of the regions of the tubular body adjoining it.
  • the curvature is the reciprocal of the radius of curvature of the respective profile.
  • the flattening results from the fact that its curvature is smaller than the curvature of the areas of the tubular body adjoining it.
  • the flattening also results from the fact that the curvature is less than the curvature of the areas of the tubular body adjoining it.
  • the flattened area is particularly suitable for identifying pipette tips.
  • the tubular body has, in a cross section on the outer circumference, at least in the areas adjoining the flattened area, an arcuate profile and the flattened area has a straight or an arcuate profile with a greater radius of curvature than the areas adjoining the flattened area.
  • the areas adjoining the flattened area are overall in the shape of a circular arc.
  • the invention also relates to embodiments in which the profile of the Flattening adjoining areas deviates from a circular arc shape, for example has an elliptical or otherwise curved course.
  • the profile of the flattening can deviate from a straight or circular arc-shaped curved course, for example have an elliptical or otherwise curved course.
  • the tubular body has a straight and / or a curved profile in all cross sections through the flattening in the flattening.
  • the tubular body has exclusively a straight profile in all cross-sections through the flattening in the flattening or exclusively a curved profile in all cross-sections through the flattening in the flattening.
  • the tubular body has in all cross-sections through the flattening in the flattening exclusively a profile curved with the same radius of curvature or in different cross-sections a profile curved with a different radius of curvature, the curvature of the profile preferably changing gradually from cross-section to cross-section.
  • the flattening has an outwardly curved profile and an inwardly curved profile in different cross-sections, the curvature of the profile preferably changing gradually from cross-section to cross-section.
  • the flattened area has an inwardly curved profile.
  • this is a profile that is inwardly curved overall with the same or different curvatures or a profile that is curved inward in the central area and a straight profile on the two lateral edges that is approximately V-shaped overall.
  • the inwardly curved flattening is preferably in one area Circumference of more than 10 °, preferably more than 30 °.
  • at least 3 flat areas with an inwardly curved contour are evenly distributed over the circumference and the flat areas with an inwardly curved contour are the same width or wider than the areas between the flat areas with an inwardly curved contour.
  • the at least one flat area extends upwards to a distance from the upper end of the tubular body.
  • an upper edge with a uniform wall thickness can be achieved, which is advantageous for ejecting a pipette tip from the attachment by means of an ejection device of the pipetting device.
  • the upper edge of the pipette tip is a circumferential flange. The flange can be used to hold a pipette tip in a hole of a holder for pipette tips (rack).
  • the flat extends to the upper end of the tubular body.
  • the extension of the flattened area up to the upper end is advantageous for the deformation of the pipette tip with reduced expenditure of force.
  • the tubular body has a shoulder on the outer circumference. With the shoulder, the pipette tip can be supported in a hole in a holder for pipette tips.
  • the flattening extends downwards at least up to the shoulder or beyond.
  • the tubular body has several, preferably three, flat areas on the outer circumference. The force required to deform the pipette tips can be further reduced by the multiple flattened areas.
  • different types of pipette tips can be identified differently by pipette tips with different numbers and / or positions and / or dimensions of the flattened areas.
  • the flats are evenly distributed over the outer circumference of the tubular body.
  • a uniform expansion of the pipette tip can hereby be achieved.
  • the flattened areas can, however, also be distributed unevenly over the outer circumference of the tubular body, in particular in order to identify different types of pipette tips differently.
  • the pipette tip according to the invention comprises in particular the following types of construction:
  • the pipette tip has at least one flat, which according to feature (i) has a straight profile in cross section through the tubular body, the pipette tip can have a single flat and a single curved adjacent area extending from one side edge of the flat to extends to the other side edge of the flat. Furthermore, the pipette tip can have a plurality of flattened areas with a straight profile in cross section through the tubular body, which are distributed over the circumference of the tubular body, the lateral edges of the flat areas being adjoined by curved regions which extend to the lateral edges of the adjacent flattened areas.
  • pipette tips are included which have a plurality of flattened areas with a cross-section through the tubular Have body rectilinear profile, wherein the flattened areas adjoin one another at the side edges, so that the flattened areas in the cross section through the tubular body define a total of a polygon with rectilinear sides.
  • the pipette tip has at least one flat which, according to feature (ii), has a profile that is less curved in cross section through the tubular body than the areas adjoining the flats
  • the pipette tip can have a single flat and a single curved adjoining area which extends from one side edge of the flat to the other side edge of the flat.
  • the pipette tip can have a plurality of flattened areas with a profile which is curved in cross section through the tubular body and which are distributed over the circumference of the tubular body, the lateral edges of the flat areas being adjoined by curved regions which extend to the lateral edges of the adjacent flattened areas.
  • the plurality of flats can all be curved in the same way or in different ways. In particular, they can all be curved with the same or different curvatures outwards or inwards or outwards and inwards.
  • the at least one flat area runs parallel to the central axis of the tubular body or helically around the central axis of the tubular body.
  • each flat extends only over a fraction of the circumference of the tubular body or once or more than once around the circumference of the tubular body.
  • the flats as a whole occupy a larger part of the outer circumference of the tubular body than those thereon in FIG A total of areas of the tubular body adjoining the circumferential direction.
  • the seat area is conical and / or cylindrical.
  • the tubular body on the inner circumference of the seat area has at least one inwardly protruding, circumferentially encircling sealing structure and / or at least one inwardly protruding, circumferentially circumferential or having several sections spaced apart from one another guide structure and / or at least one inwardly protruding , circumferential or a plurality of spaced apart sections pointing braking structure.
  • the sealing structure is a sealing bead and / or the guide structure is a guide bead and / or a guide rib and / or knob-shaped or lug-shaped guide projections and / or the braking structure is a braking bead and / or a conical one Braking range.
  • the sealing structure causes a ring support between the attachment of the pipette device and the pipette tip and thus a good seal with low frictional forces when the pipette tip is attached to the attachment.
  • the seat area can have a surface seal which is formed, for example, by a conical or cylindrical surface or a surface that is conical in sections and cylindrical in sections.
  • the guide structure causes a ring support or several circular arch-shaped supports spaced apart from one another in the circumferential direction and / or several approximately point-shaped supports spaced apart from one another in the circumferential direction between the attachment of the pipetting device and the pipette tip and thus good guidance with low friction forces when the pipette tip is attached to the attachment.
  • the guide structure holds the pipette tip in a stable manner at the attachment, even if a force is introduced from the side into the lower end of the pipette tip when it is dispensed from the wall (liquid is dispensed on a vessel wall).
  • the braking structure causes a ring support or an annular arch support or a ramp-shaped support between the attachment and the pipette tip and brakes the insertion movement of the attachment into the pipette tip.
  • the braking effect is determined by the geometry of the braking structure and the attachment and the material properties (especially elasticity and roughness) of the pipette tip and attachment.
  • the guide structure is arranged above the sealing structure and / or the braking structure is arranged below the sealing structure.
  • the guide structure coincides with the sealing structure and / or the sealing structure coincides with the braking structure.
  • a guide bead can also be designed as a sealing bead and / or a sealing bead can also be designed as a friction bead.
  • the tubular body has a plurality of sealing structures and / or guide structures and / or friction structures on the inner circumference, which have a wave-shaped contour in a longitudinal section through the tubular body.
  • the tubular body has a widening at the upper opening and / or an insertion bevel on the inner circumference. The widening and / or insertion bevel facilitates the insertion of a tip of a pipetting device into the pipette tip.
  • the pipette tip consists exclusively of the tubular body.
  • the pipette tip is an air-cushioned pipette tip, i.e. it is designed to be used with an air-cushioned pipetting device.
  • the air-cushion pipette tip consists exclusively of the tubular body.
  • the pipette tip consists of the tubular body and a further component.
  • the further component is, for example, a small piston which is arranged within the tubular body and can be displaced therein.
  • This is a positive displacement pipette tip, i.e. a pipette tip that can be used with a positive displacement pipetting device.
  • the pipette tip is made from at least one thermoplastic, preferably from at least one polyolefin, preferably from at least one polypropylene and / or polyethylene.
  • the pipette tip has one or more of the following features: the wall thickness of the tubular body in the circumferential direction next to the at least one flat area falls in the range from 0.3 to 1 mm, the seat area is conical on the inside with a downwardly decreasing diameter, the cone angle of the seat area being selected from the range from 1 ° to 6 °, preferably from 1.5 ° to 2.5 °, the seat area is designed to be slipped onto an attachment, the cone angle of the conical attachment or of the conical section of the attachment being selected from the range from 1.0 ° to 10 °, preferably from the range from 1.3 ° to 7 °, further preferably from the range from 1.5 ° to 3 °, the sealing structure and / or guide structure and / or braking structure are distributed in the longitudinal direction of the tubular body over the seat area , The wall thickness of the tubular body in the area of the flattening (outside the sealing structure and / or guide structure and / or braking structure) at the thinnest point
  • the invention further relates to a pipette tip system comprising a plurality of pipette tips according to one of claims 1 to 16 or one of the preceding types of embodiment, with pipette tips of different pipette tip types have differently designed flat areas and / or different markings on the flat areas.
  • the invention also relates to a pipetting system with at least one pipette tip according to one of claims 1 to 16 or one of the preceding embodiments and a single-channel pipetting device with a single attachment for attaching a pipette tip and / or a multi-channel pipetting device with several attachments for simultaneously attaching several pipette tips.
  • the terms “vertical” and “horizontal”, “top” and “bottom” as well as terms derived therefrom such as “above” and “below” relate to an arrangement of the pipette tip with a vertically aligned central axis of the tubular body, whereby the top opening is at the top and the bottom opening is at the bottom.
  • each cross section through the tubular body is a plane oriented perpendicular to the central axis of the tubular body.
  • Each longitudinal section through the tubular body is a plane in which the central axis of the tubular body extends.
  • the central area of the flat is a line or a strip-shaped zone which runs between the two side edges of the flat, wherein the line or zone can have the same distances from the two side edges of the flat or different distances from the two side edges of the May have flattening.
  • a pipette tip 1 has an elongated, tubular body 2 which has a lower opening 4 at the lower end 3 and an upper opening 6 at the upper end 5.
  • the lower opening 4 is smaller than the upper opening 6.
  • the inner and outer diameter of the tubular body 2 increases from the lower opening 4 to the upper opening 6.
  • the tubular body 2 has at the bottom a conical starting section 7, above a middle section 8 with a smaller cone angle than the starting section 7 and above it a cylindrical head portion 9 with larger dimensions than the central portion 8. Adjacent to the center from section 8 runs on the underside of Kopfab section 9 on the outer circumference of the tubular body 2, a downwardly directed outer shoulder 10 around.
  • Flattened areas 12 extending in the axial direction are provided on the outer circumference 11 of the head section.
  • the flats 12 begin at a small distance from the upper end 5 of the tubular body 2 and extend downward over the head section 9 to a little over the shoulder 10 and into the upper area of the central section 8.
  • the flats 12 are aligned parallel to the central axis of the tubular body 2.
  • the flats 12 are arranged distributed uniformly around the outer circumference 11 of the tubular body.
  • the flattened areas 12 are each approximately the same width as the areas 13 adjoining them.
  • the tubular body 2 has in each cross section through the flats 12 on the outer circumference 11 in the areas 13 adjoining the flats 12 an arcuate profile 14 and in the flats 12 a straight profile 15.
  • the arcuate profiles 14 extend over the entire distance range between two adjacent flattened areas 12, that is to say between the flattened areas 12, the profiles 14 are arc-shaped everywhere. This is shown in particular in FIG. 3.
  • the tubular body 2 has a circumferential edge 16 with a uniform wall thickness. At the upper end, the tubular body 2 has a widening 18 with an insertion bevel 19 on the inner circumference 17. This is shown in particular in FIG. 4.
  • the tubular body 2 has on the inner circumference 17 a substantially conical or cylindrical seat area 20 for a conical or cylindrical extension 21 of a pipetting device.
  • the seat area 20 extends into the head section 9 and is conical with a cone angle of, for example, 2 ° to 6 °.
  • the tubular body 2 has on the inner circumference 17 in the seat area 20 a plurality of sealing structures 22 in the form of inwardly projecting, closed circumferential sealing beads 23 which are spaced apart from one another in the axial direction. Furthermore, the tubular body 2 has, at a smaller distance from the upper opening 6, inwardly projecting, closed circumferential guide structures 24 in the form of guide beads 25 which are spaced apart from one another. Overall, the tubular body 2 in the seat area 20 has wave-shaped sealing and guide structures 22, 24 in longitudinal section.
  • a conical braking area 26 is provided on the inner circumference of the head section 9.
  • the inner contour of the tubular body 2 merges smoothly into the downwardly tapering middle section 8, which merges further down into the starting section 7.
  • the tubular body 2 has a constant wall thickness everywhere in cross section in the adjoining areas 13 between the flattened areas 12. Furthermore, the wall thickness of the tubular body 2 in the flattened areas 12 decreases in each case starting from the areas 13 adjoining them towards the central area 27 of the flattened areas 12.
  • the flats 12 are provided with markings 28. These are inscriptions 29 in the form of raised or recessed numbers and letters that are produced during the injection molding of the pipette tip 1.
  • the inscriptions 29 indicate the size of the pipette tip and the nest of the injection molding tool in which the pipette tip 1 is injection molded.
  • one or more pipette tips 1 can be kept ready in holes of a holder for pipette tips, with their shoulder 10 being supported on the edge of the holes.
  • the extension 21 of a pipetting device can easily be introduced into the pipette tip 1 through the widening 18 with the insertion bevel 19 at the upper opening 6. In it, it is guided with little friction by the guide structures 24 and sealing structures 22.
  • the pipette tip 1 can be elastically and / or plastically deformed in the area of the flattened areas 12, whereby the attachment forces are reduced and a secure seal of the attachment 21 on the sealing structures 22 is achieved with relatively lower attachment forces.
  • the pipette tip 1 After the pipetting of liquid, the pipette tip 1 can easily be thrown off the attachment 21, since the ejection forces to be applied for ejection are also reduced. For ejection, an ejection sleeve of the pipetting device guided on the attachment 21 is pressed against the circumferential edge 16 at the upper end 5 of the pipette tip 1 and the pipette tip is stripped off the attachment 21.
  • the pipette tip 1 of FIGS. 6 to 10 is preferably designed for pipetting smaller filling volumes than the pipette tip 1 of FIGS. 1 to 5.
  • the pipette tip 1 differs from the one described above in particular in that the elongated, tubular body 2 has a plurality of conical middle sections 8.1, 8.2, 8.3 above the conical starting section 7, a conical transition section 30 above and a conical head section 9 with a circumferential, radially following has externally projecting flange 31 at the upper end 5.
  • the aforementioned sections 7, 8.1, 8.2, 8.3, 40 and the flange 31 directly adjoin one another.
  • the outer diameter of the tubular body 2 increases gradually from the lower end 3 to the upper end of the head section 9.
  • the inner diameter of the tubular body 2 also increases gradually from the lower end 3 to the upper end 5 of the tubular body 2.
  • This pipette tip 1 has no shoulder between the central section and the head section.
  • the flange 31 has on the underside downwardly projecting ribs 32 which, starting from the head section 9, extend radially outward.
  • the flattened areas 12 extend from the underside of the flange 31 in the axial direction of the tubular body 2 to the upper edge area of the transition section 30.
  • This pipette tip 1 also has three flat flat areas 12.
  • the adjoining areas 13 of the head section 9 each have an arcuate profile in cross section, which extends over the entire area between two adjacent flattened areas 12.
  • One or more pipette tips 1 according to FIGS. 6 to 10 can be provided in a holder for pipette tips. They are inserted into holes in the holder and supported with the ribs 32 on the underside of the flange 31 on the edge of the holes. According to FIG. 10, the extension 21 is only partially pushed into the pipette tip 1 as far as the uppermost guide bead 25.
  • the pipette tip 1 has a further guide bead 25 and below it a sealing bead 23.
  • the attachment is pushed forward up to the sealing bead 23 so that the pipette tip 1 is aligned and clamped onto the attachment 21 in a sealing manner.
  • the guide beads 25 also contribute to the sealing of the pipette tip 1 on the attachment 21.
  • the sealing bead 23 is at the same time a braking bead which brakes the introduction of a projection into the pipette tip 1.
  • the braking effect is determined by the bevel at the upper edge and the rounding at the apex of the sealing bead 23.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne une pointe de pipette en matière plastique avec une forme allongée, un corps tubulaire avec une ouverture inférieure à l'extrémité inférieure pour le passage de liquide et une ouverture supérieure au niveau de l'extrémité supérieure pour un serrage sur une fixation d'un dispositif de pipette. Ensuite, l'ouverture supérieure sur la circonférence interne du corps tubulaire, il est prévu une région de siège pour la fixation, et à proximité de l'ouverture supérieure sur la circonférence externe du corps tubulaire, au moins une partie aplatie s'étendant dans la direction axiale. L'épaisseur de la paroi du corps tubulaire dans une section transversale à travers le corps tubulaire à partir de l'une ou l'autre des deux régions du corps tubulaire adjacente à la partie aplatie diminue progressivement dans la partie aplatie vers sa région centrale, et la partie aplatie dans la section transversale à travers le corps tubulaire a un profil droit ou un profil qui est incurvé dans une moindre mesure que les régions adjacentes.
EP21714190.2A 2020-04-03 2021-03-26 Pointe de pipette Pending EP4100164A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP20167992.5A EP3888791A1 (fr) 2020-04-03 2020-04-03 Point de pipette
PCT/EP2021/057967 WO2021198086A1 (fr) 2020-04-03 2021-03-26 Pointe de pipette

Publications (1)

Publication Number Publication Date
EP4100164A1 true EP4100164A1 (fr) 2022-12-14

Family

ID=70189716

Family Applications (2)

Application Number Title Priority Date Filing Date
EP20167992.5A Withdrawn EP3888791A1 (fr) 2020-04-03 2020-04-03 Point de pipette
EP21714190.2A Pending EP4100164A1 (fr) 2020-04-03 2021-03-26 Pointe de pipette

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP20167992.5A Withdrawn EP3888791A1 (fr) 2020-04-03 2020-04-03 Point de pipette

Country Status (4)

Country Link
US (1) US20230138840A1 (fr)
EP (2) EP3888791A1 (fr)
JP (1) JP2023520982A (fr)
WO (1) WO2021198086A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102022120212A1 (de) 2022-08-10 2024-02-15 Hamilton Bonaduz Ag Pipettierspitze mit sich gekrümmt verjüngendem Aufnahmeraum

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6197259B1 (en) 1998-11-06 2001-03-06 Rainin Instrument Co., Inc. Easy eject pipette tip
US6568288B2 (en) 2001-06-29 2003-05-27 Rainin Instrument, Llc Pipette with improved pipette tip and mounting shaft
US7335337B1 (en) 2001-09-11 2008-02-26 Smith James C Ergonomic pipette tip and adapters
US6967004B2 (en) 2001-10-30 2005-11-22 Rainin Instrument, Llc Pipette with improved pipette tip and mounting shaft
EP2138234A1 (fr) * 2008-06-24 2009-12-30 F. Hoffmann-Roche AG Interface souple de pointe jetable
WO2011091308A2 (fr) 2010-01-22 2011-07-28 Biotix, Inc. Pointes de pipette
EP2606977B1 (fr) * 2011-12-23 2019-07-03 Eppendorf AG Embout de pipette
EP3115110B1 (fr) 2015-07-07 2019-03-27 Eppendorf Ag Pointe de pipette
WO2018213196A1 (fr) 2017-05-17 2018-11-22 Biotix, Inc. Embouts de pipette ergonomiques
JP6539774B1 (ja) * 2018-05-11 2019-07-03 バイオティクス， インコーポレイテッド ピペットチップ

Also Published As

Publication number Publication date
WO2021198086A1 (fr) 2021-10-07
EP3888791A1 (fr) 2021-10-06
JP2023520982A (ja) 2023-05-23
CN115397557A (zh) 2022-11-25
US20230138840A1 (en) 2023-05-04

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