EP2606977B1 - Embout de pipette - Google Patents

Embout de pipette Download PDF

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Publication number
EP2606977B1
EP2606977B1 EP11010189.6A EP11010189A EP2606977B1 EP 2606977 B1 EP2606977 B1 EP 2606977B1 EP 11010189 A EP11010189 A EP 11010189A EP 2606977 B1 EP2606977 B1 EP 2606977B1
Authority
EP
European Patent Office
Prior art keywords
pipette tip
ribs
area
sealing
attachment piece
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP11010189.6A
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German (de)
English (en)
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EP2606977A1 (fr
Inventor
Jochen Beese
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Priority to EP11010189.6A priority Critical patent/EP2606977B1/fr
Priority to JP2012275879A priority patent/JP5775059B2/ja
Priority to CN201210559958.5A priority patent/CN103170386B/zh
Publication of EP2606977A1 publication Critical patent/EP2606977A1/fr
Application granted granted Critical
Publication of EP2606977B1 publication Critical patent/EP2606977B1/fr
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/08—Ergonomic or safety aspects of handling devices
    • B01L2200/087—Ergonomic aspects

Definitions

  • the invention relates to a pipette tip.
  • Pipette tips are used with pipettes and other dosing devices to dose liquids.
  • pipettes and metering devices are collectively referred to as "pipetting device”.
  • Pipette tips have an elongate, tubular body having a pipetting opening at the lower end and an attachment opening for clamping onto a lip of a pipetting device at the upper end.
  • the pipetting device comprises a gas displacement device, which is usually a piston with a cylinder axially displaceable therein.
  • the gas displacement device is connected to a through hole of the neck.
  • the pipette tip is clamped by pressing the approach of the pipette into the upper opening of the pipette tip sealingly on the approach.
  • an air column is displaced to suck liquid into the seated on the approach pipette tip or ejected from this. If the air column is displaced away from the pipette tip, it will a certain amount of liquid is sucked through the lower opening in the pipette tip. By moving the air column toward the pipette tip, an amount of liquid is released through the lower opening from the pipette tip.
  • Pipetting devices usually have an ejector, which acts on the upper edge of the pipette tip to squeeze them from the approach. This avoids the user having to touch the contaminated pipette tip to pull it off the neck.
  • the pipetting device may be a handheld pipette that the user can hold and manipulate with one hand.
  • the pipetting device can also be a dosing station or integrated into a workstation.
  • the gas displacement device may be manual or motorized.
  • the plugging and dropping of the pipette tip can also be done manually or motor driven.
  • the pipette tip To avoid incorrect pipetting, the pipette tip must be sealingly fixed on the shoulder. In addition, the forces for attaching and dropping the pipette tip from the neck should not be too high.
  • the contact area between the pipette tip and the conical shoulder is frusto-conical.
  • the pipette tip When attaching the pipette tip, the pipette tip is stretched elastically on the circumference by the approach. The spring characteristic is steep, so high Aufsteck mechanism are applied. After fitting, a correspondingly high static friction acts between the attachment and the pipette tip. This static friction must be overcome when the pipette tip is dropped. The user is burdened by the high forces for attaching and dropping the pipette tip. As far as the plugging and dropping By means of motor drives, these must be correspondingly powerful and have a high power consumption.
  • the US 6,197,259 B1 describes a pipette tip that can be easily and firmly mounted on a neck of a pipette and easily dropped from it by the use of relatively small axial pinning and ejection forces of six and three pounds (26.7N and 13.3N, respectively).
  • the pipette tip has a tubular pipette section which has a conical upper end with an inner diameter at its upper end that is larger than the diameter of the lip of the pipette onto which the pipette tip is to be mounted. Further, the pipette tip has a hollow central portion and an annular sealing portion at the junction between the upper end and the central portion.
  • the annular sealing area has an inner diameter that is smaller than a value "x" and is designed to engage the lower end of a seal zone of the lug so as to be stretched radially outward when the lug is inserted, whereby a liquid-tight seal between the sealing zone of the neck and the sealing area of the pipette tip is generated.
  • the pipette tip on the inside adjacent to the sealing region has lateral stabilizing means which engage the outer surface of the neck to stabilize the pipette tip on the neck. These lateral stabilizing means comprise at least three circumferentially spaced contacts extending inwardly from the inner surface of the pipette tip.
  • the diametrical spacing of the contacts is sized to easily engage the free end of the lug and allow the lower end of the lug to pass without flaring the side walls of the pipette tip on which the contacts are located.
  • the contacts provide lateral support of the pipette tip on the neck to prevent the pipette tip from lateral movement when lateral outward Forces acting on the pipette tip, such as the removal of liquid on a wall.
  • the lower end of the seal zone of the lug engages the seal of the pipette tip, the pipette tip is stretched in the seal and immediately adjacent thereto.
  • the contacts guide the tip onto the lug and align it stably and securely on the lug, the side wall of the pipette tip carrying the contacts deforms inward and does not expand, thereby applying minimal force to the lug upon insertion.
  • the US Pat. No. 7,335,337 B1 describes an ergonomically designed pipette tip which can be securely attached to a shaft of a pipette, while at the same time the axial force required for attachment and ejection is considerably reduced.
  • the ergonomic pipette tip includes injected thin baffles flanked by thicker wall segments, and / or a second resilient material to reduce the force required to stretch the top edge of the pipette tip as the pipette shaft penetrates into the sealing area of the pipette tip is introduced.
  • the pipette tip has on its inside one or more projections which are intended to limit the insertion depth of the pipette shaft into the pipette tip as a stopper.
  • the EP 1 520 624 B1 describes a pipette tip made of plastic with an elongated, tubular body having a pipetting opening at a lower end and at an upper end an insertion opening for attachment to a receiving shaft of a pipetting device.
  • the pipette tip has near the upper end a circumferential curve along a closed curve, in different circumferential positions different distances from the lower end on the web.
  • the circumferential ridge has a contact area on the inside, which revolves on a surface of revolution. More bridges extend from the maximum of the lower end remote locations of the circumferential ridge to the upper end.
  • the pipette tip comes with the contact area on the inside of the circumferential ridge in sealing contact with the neck of a pipetting device.
  • the ejector of the pipetting device presses against the further webs at the upper end of the pipette tip.
  • the ejection force is transmitted to the maximum remote from the lower end points of the circumferential ridge, As a result, these points of the circumferential ridge are pressed slightly towards the lower end.
  • the circumferential ridge is pressed slightly flatter.
  • the further webs forms a stiff region, which is subjected to only a relatively small deformation upon transmission of forces. Since the circumferential web is aligned transversely to the other webs, it is relatively easily deformed by the forces introduced in the direction of the further web.
  • the circumferential ridge widens slightly and accordingly the static friction between the inside of the circumferential ridge or the contact area and the neck is reduced.
  • the forces required to disengage the pipette tip from the receiving shaft are reduced.
  • the pipette tip is more flexible in the area of the circumferential web, so that the forces for plugging are reduced to one approach and the attachment to receiving shafts with different dimensions is favored.
  • the EP 2 138 234 A1 describes a pipette tip made of plastic, which has a meandering cross-section above the sealing region with inwardly and outwardly pointing ribs to allow a radial expansion.
  • the object of the invention is to provide a pipette tip in which the required ejection force for ejecting the pipette tip from the attachment of a pipetting device is reduced and dimensional tolerances have a smaller influence on the ejection force.
  • the pipette tip according to the invention is in the form of an elongate tube having a lower opening at the lower end for the passage of liquid and an upper opening at the upper end, wherein adjacent to the upper opening at the inner periphery there is a seating area which is adapted for attachment to a standardized serves a conical approach of a pipetting device, wherein the Silz Scheme a holding portion with radially inwardly projecting, axially extending ribs and below the holding portion has a sealing region and is formed so that when attaching the pipette tip with the seating area on the approach with a Aufsteckkraft, a holding and sealing the pipette tip on the approach ensures that the ribs are partially plastically deformed and outside of the ribs in the seating area enters an elastic deformation.
  • the surface pressure in the region of the axial ribs causes a partial plastic deformation of the axial ribs.
  • the pipette tip is elastically deformed in the seating area outside the axial ribs. A portion of the Aufsteckkraft is consumed for the plastic deformation of the ribs and another part of the Aufsteckkraft is stored in the elastic deformation.
  • the pipette tip is held under tension on the approach.
  • the ribs support the pipette tip laterally so that it does not wobble under the influence of lateral forces. To eject the pipette tip, only the force stored by the elastic deformation has to be overcome.
  • the attributable to the plastic deformation of the axial ribs part of Aufsteckkraft does not need to be overcome again as a stored force when ejecting the pipette tip.
  • the ejection force must overcome the frictional force that exists due to the elastic bias between the pipette tip and the neck.
  • Dimensional tolerances of the pipette tip and neck are compensated for by the plastic deformation of the ribs and have less effect on the elastic deformation in the seating area than in conventional pipette tips. As a result, the influence of dimensional tolerances on the ejection force is reduced.
  • the elastic deformation in the seating area can take place in the sealing area and / or in the holding area between the ribs.
  • the elastic deformation may be restricted to the sealing area or to the area between the ribs, or may take place both in the sealing area and in the area between the ribs.
  • the plastic deformation of the ribs and the elastic deformation in the seating area is determined by the geometry of the seating area and the material of the pipette tip.
  • the plastic deformation of the ribs is determined in particular by the rib profile.
  • the wall thickness of the pipette tip in the area of the ribs determines the degree of elastic deformation in this area.
  • the wall thickness in the region of the ribs can be chosen so large that the pipette tip behaves rigidly there and only a plastic deformation occurs at the ribs.
  • An elastic or plastic deformation in the sealing region can be determined, in particular, by the wall thickness of the pipette tip in the sealing region and, if necessary, by the geometry of a sealing projection running around the inside of the seal.
  • the ribs are distributed uniformly over the circumference. This promotes uniform deformation and support of the pipette tip in the seating area.
  • the pipette tip has three to twelve ribs, preferably five to seven ribs, furthermore preferably six ribs. As a result, a uniform support is achieved.
  • the mentioned numbers of ribs favor plastically deformable ribs with dimensions that are easy to manufacture.
  • the ribs are dimensioned so that their adoptedGermanlappung with the standardized conical approach relative to the position taken by the approach when the pipette tip is attached with a Aufsteckkraft on the approach, the holding and sealing of the pipette tip on the Guaranteed approach, at least 0.1 mm, preferably at least 0.2 mm and / or at most 0.5 mm, preferably at most 0.3 mm.
  • This dimensional overlap favors a partial plastic deformation of the ribs.
  • the pipette tip next to the upper opening has inwardly projecting, axially extended further ribs, which extend less far radially inwardly than the ribs, wherein the further ribs are each arranged between two ribs.
  • the further ribs can serve to guide the pipette tip in addition to the approach, wherein the further ribs when plugging the pipette tip can be subjected to the approach no deformation or only a smaller deformation than the ribs.
  • the further ribs are dimensioned so that they are not deformed when the pipette tip with a Aufsteckkraft on the Attached approach that ensures holding and sealing the pipette tip on the approach.
  • the ribs and / or the further ribs have a radially inwardly tapering profile. This promotes plastic deformation of the axial ribs at the inner end of the profile.
  • the ribs and / or the further ribs have a triangular or a trapezoidal or a parabolic profile.
  • the ribs have a profile with a structured surface, e.g. a profile formed by nubs, pins or needles or occupied therewith.
  • the profiles are used, for example, the stiffening of the pipette tip in the rib area or the marking of the pipette tip. If they serve to identify the pipette tip, the arrangement of the profilings over the ribs and / or the further ribs promotes the recognizability of the profilings on a transparent pipette tip. Otherwise, the ribs and / or the other ribs could be perceived as profilings.
  • the pipette tip expands in the region in which the ribs are arranged, and / or conically in the sealing region towards the upper opening.
  • the regions mentioned may have a circular cylindrical shape instead of a conical if the ribs are adapted by plastic deformation to the standardized conical approach and in the sealing area circumferential sealing projection is present, with which the sealing area abuts the approach.
  • the sealing region has an inwardly extending on the circumference, inwardly projecting sealing projection.
  • the sealing projection is for example a sealing bead or a sealing lip.
  • the pipette tip in the region in which the ribs are arranged has a wall thickness which is greater than its wall thickness in the sealing region.
  • the pipette tip has means for limiting the attachment to the standardized conical projection, which stop the attachment to the approach when a Aufsteckkraft is reached, the holding and sealing the pipette tip on the neck and a partial plastic deformation of the Ribs guaranteed.
  • the means for limiting may be formed by a stop which may be formed by one or more projections which project inwardly from the inner circumference of the pipette tip.
  • the means for limiting the Aufstecken s a under the sealing area conically widening towards the upper opening towards the brake area.
  • the lower edge of the approach meets the brake area and the insertion of the approach is by the taper of the braking area gradually stopped.
  • the user is less stressed than in a conventional stop, which stops the Aufsteckterrorism abruptly.
  • the pipette tip is made of a plastic. Further preferably, the pipette tip is made of a polyolefin, preferably polypropylene or polyethylene. According to a further embodiment, the pipette tip is translucent, preferably transparent. Further preferably, the pipette tip is made by injection molding. Preferably, the inner and / or outer diameter of the pipette tip increases from the lower opening to the upper opening.
  • top and bottom refer to the orientation of a pipette tip during pipetting in which the pipette tip is vertically aligned and the bottom opening is at the bottom and the top opening is at the top.
  • a pipette tip 1 has an elongate, tubular body 2 which has a lower opening 4 at the lower end 3 and an attachment opening 6 at the upper end 5.
  • the lower opening 4 is smaller than the upper opening 6.
  • the inner and / or outer diameter of the tubular body 2 increases from the lower opening 4 toward the upper opening 6.
  • the tubular body 2 below a conical initial section 7, about a less conical central portion 8 and above a head portion 9.
  • a pipette tip 1 according to the invention can also extend in other ways from the lower opening 4 to the upper opening 6, especially in sections or steadily.
  • the head portion 9 has, immediately adjacent to the upper opening 6, an enlarged diameter insertion area 10 that extends only over a short portion of the head portion 9.
  • a holding region 11 adjoins, in the ribs 12, 13 protrude inwardly from the inner periphery of the head portion.
  • the Ribs 12 project further from the inner periphery of the pipette tip 1 as the other ribs 13.
  • six ribs 12 and six further ribs 13 are present. Between each two ribs 12 each have a further rib 13 is arranged.
  • the head portion 9 has at the top an outwardly projecting frusto-conical portion 16. This is bounded below by an outer step 17. Inside, the head portion has an inner step 18. The ribs 12 and the further ribs 13 extend beyond the inner step 18. Below they end in a sealing region 19. There is inside of the circumference of the head portion 9 before a circumferential sealing projection 20 in the form of a bead. The ribs 12 and the further ribs 13 extend up to the sealing projection 20. In the region of the inner step 18, the further ribs 13 have a discontinuity 21.
  • the ribs 12 and the further ribs 13 have a parabolic profile 22, 23 in the region above the inner step 18.
  • the profile has a flattening or a flat radius 24, 25 at the furthest inwardly projecting region. In the direction from top to bottom, first the parabolic profile 22, 23 widens to a point 26, 27 of maximum width, from where it becomes narrower again up to the inner step 18.
  • the head area outside the circumference of the frusto-conical region 16 has teardrop-shaped, outwardly projecting profiles 28.
  • the edges of the profiles 28 coincide with the edges of the ribs 12 and the further ribs 13 above the inner step 18th
  • a conical braking region 29 connects to the sealing region 19, which tapers downwards or widens upwards.
  • the head section 9 is slightly conical inside above the inner step 18 and below the inner step 18 and in the sealing region 19. In the braking area 29, it is more conical.
  • sealing area 30 The area of the further ribs 13, the sealing area 19 and the braking area 29 are collectively referred to as "seating area" 30.
  • the middle section 8 limits the body 2.
  • a manual pipette 31 has a housing 32 in the form of a handle, which contains a displacement device 33 for displacing air and to which an actuating element 34 for actuating the displacement device 33 and an ejector 35 with an associated further actuating element 36 are arranged.
  • a standardized conical projection 37 is arranged on the housing 32.
  • a pipette tip 1 is attachable to the neck 37.
  • Fig. 3 When plugging the pipette tip 1 with the seating area 30 on the standardized conical projection 37, the ribs 12 are partially plastically deformed. This is in Fig. 3 in the overlap region of the contours of the projection 37 and the ribs 12 given. Between the ribs 12 and the further ribs 13, the wall of the pipette tip 1 is slightly elastically deformed. In the region of the sealing projection 20 of the seating area 30 is elastically deformed by the wall slightly outwardly with respect to the position shown is deflected, in which the contours of the sealing projection 20 and the projection 37 overlap each other.
  • the brake area 29 is plastically deformed. This is where the contours of the lower extension end 38 and the braking region 29 overlap.
  • the ejection force is lower than in the case of a conventional pipette tip, which is exclusively elastically deformed by the attachment force.
  • Convention pipette tips Aufsteckkraft and ejection force match when the pipette tip is attached with little physical effort. This is in Fig. 4b shown.
  • the release force is even greater than the attachment force.
  • Fig. 5 a . b shows, respectively, the mean diameter and the cone angle of a standardized conical projection 37 over an associated pipette tip 1 with a certain tip size.
  • the mean diameter is given in millimeters and the cone angle in degrees.
  • As a measure of the pipette tip size is respectively the area of the filling volume in microliter or milliliter specified, with the pipette tip 1 is filled.
  • the median diameter of the lug 37 refers to a diameter between the lower and upper ends of the lug 37. It need not necessarily be a diameter located exactly midway between the lower and upper ends of the lug. However, the average diameter may also fall exactly in the middle between the lower end and the upper end of the neck.
  • Fig. 5 a . b the tolerances are given for the mean diameter (+/- 0.05mm) and for the angle data (+/- 0.3 °).
  • the lugs 37 are manufactured with appropriate tolerances.

Claims (15)

  1. Pointe de pipette en plastique présentant la forme d'un petit tube oblong avec une ouverture inférieure (4) à l'extrémité inférieure (3) pour le passage de liquide et une ouverture supérieure (6) à l'extrémité supérieure, dans laquelle une région de siège (30) est prévue sur le pourtour intérieur à côté de l'ouverture supérieure (6), laquelle est destinée à être montée sur un embout conique standardisé (37) d'un dispositif de pipetage (31), dans laquelle la région de siège (30) présente une région de maintien (11) avec des nervures (12) s'étendant axialement et faisant saillie radialement vers l'intérieur ainsi qu'une région d'étanchéité (19) sous la région de maintien (11), tout en étant conçue de telle façon que lors du montage de la pointe de pipette (1) avec la région de siège (30) sur l'embout (37) avec une force de montage garantissant un maintien et une étanchéité de la pointe de pipette (1) sur l'embout (37), les nervures (12) sont déformées partiellement de façon plastique et une déformation élastique apparaît à l'extérieur des nervures (12) dans la région de siège (30), l'embout présentant les dimensions suivantes : Taille de pointe de pipette Diamètre moyen Angle conique 1.000 - 10.000 µl 14,3 mm 4,35° 100 - 5.000 µl 12,4 mm 4,00° 500 - 2.500 µl 9,3 mm 3,78° 50 - 1.250 µl 7,1 mm 2,48° 50 - 1.000 µl 7,1 mm 2,71° 20 - 300 µl 4,4 mm 6,99° 2 - 200 µl 4,4 mm 6,99° 0,5 - 20 µl 3,3 mm 6,68° 0,1 - 20 µl 2,95 mm 4,77° 0,1 - 10 µl 2,95 mm 4,77°
    où une tolérance de +/- 0,05 mm s'applique pour le diamètre moyen et une tolérance de +/- 0,3° s'applique pour l'angle conique.
  2. Pointe de pipette selon la revendication 1, dans laquelle les nervures (12) sont réparties de façon régulière sur le pourtour.
  3. Pointe de pipette selon la revendication 1 ou 2, laquelle présente entre trois et douze nervures (12), de préférence entre cinq et sept nervures, et encore de préférence six nervures.
  4. Pointe de pipette selon l'une des revendications 1 à 3, dans laquelle les nervures (12) sont dimensionnées de telle façon que leur recoupement dimensionnel avec l'embout conique standardisé (37) par rapport à la position prise par l'embout (37) lorsque la pointe de pipette (1) est montée sur l'embout (37) avec une force de montage garantissant un maintien et une étanchéité de la pointe de pipette (1) sur l'embout (37) mesure au moins 0,1 mm, de préférence au moins 0,2 mm et/ou 0,5 mm au maximum, de préférence 0,3 mm au maximum.
  5. Pointe de pipette selon l'une des revendications 1 à 4, laquelle présente à l'intérieur d'autres nervures (13) s'étendant axialement et faisant saillie vers l'intérieur à côté de l'ouverture supérieure (6), lesquelles s'étendent moins loin radialement vers l'intérieur que les nervures, les autres nervures étant disposées respectivement entre deux nervures.
  6. Pointe de pipette selon la revendication 5, dans laquelle les autres nervures (13) sont dimensionnées de telle façon qu'elles ne sont pas déformées lorsque la pointe de pipette (1) est montée sur l'embout (37) avec une force de montage garantissant un maintien et une étanchéité de la pointe de pipette (1) sur l'embout (37).
  7. Pointe de pipette selon l'une des revendications 1 à 6, dans laquelle les nervures (12) et/ou les autres nervures (13) présentent un profil s'affinant radialement vers l'intérieur.
  8. Pointe de pipette selon la revendication 7, dans laquelle les nervures (12) et/ou les autres nervures (13) présentent un profil triangulaire ou trapézoïdal ou parabolique.
  9. Pointe de pipette selon l'une des revendications 1 à 8, laquelle présente des profilages (28) recouvrant les nervures (12) et/ou les autres nervures (13) à l'extrémité sur le pourtour.
  10. Pointe de pipette selon l'une des revendications 1 à 9, laquelle s'élargit de façon conique vers l'ouverture supérieure dans la région dans laquelle se trouvent les nervures (12) et/ou dans la région d'étanchéité (19).
  11. Pointe de pipette selon l'une des revendications 1 à 10, laquelle présente, dans la région d'étanchéité (19), une saillie d'étanchéité (20) périphérique sur le pourtour, laquelle fait saillie vers l'intérieur.
  12. Pointe de pipette selon l'une des revendications 1 à 11, laquelle présente une épaisseur de paroi supérieure dans la région de maintien (11) par rapport à son épaisseur de paroi dans la région d'étanchéité (19).
  13. Pointe de pipette selon l'une des revendications 1 à 12, laquelle présente des moyens de limitation (29) du montage sur l'embout conique standardisé (37), lesquels bloquent le montage sur l'embout (37) lorsqu'une force de montage garantissant un maintien et une étanchéité de la pointe de pipette (1) sur l'embout (37) ainsi qu'une déformation partiellement plastique des nervures (12) est atteinte.
  14. Pointe de pipette selon la revendication 13, dans laquelle les moyens de limitation (29) du montage consistent en une région de freinage (29) s'élargissant de façon conique vers l'ouverture supérieure sous la région d'étanchéité (19).
  15. Pointe de pipette selon l'une des revendications 1 à 14, laquelle est fabriquée à partir d'une polyoléfine, et de préférence à partir de polypropylène ou de polyéthylène.
EP11010189.6A 2011-12-23 2011-12-23 Embout de pipette Active EP2606977B1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP11010189.6A EP2606977B1 (fr) 2011-12-23 2011-12-23 Embout de pipette
JP2012275879A JP5775059B2 (ja) 2011-12-23 2012-12-18 ピペットチップ
CN201210559958.5A CN103170386B (zh) 2011-12-23 2012-12-21 移液管尖端

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP11010189.6A EP2606977B1 (fr) 2011-12-23 2011-12-23 Embout de pipette

Publications (2)

Publication Number Publication Date
EP2606977A1 EP2606977A1 (fr) 2013-06-26
EP2606977B1 true EP2606977B1 (fr) 2019-07-03

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Family Applications (1)

Application Number Title Priority Date Filing Date
EP11010189.6A Active EP2606977B1 (fr) 2011-12-23 2011-12-23 Embout de pipette

Country Status (3)

Country Link
EP (1) EP2606977B1 (fr)
JP (1) JP5775059B2 (fr)
CN (1) CN103170386B (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4140588A1 (fr) 2021-08-30 2023-03-01 Tecan Trading AG Extrémité de pipette avec deux étapes d'angle positif

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9486803B2 (en) 2010-01-22 2016-11-08 Biotix, Inc. Pipette tips
US8795606B2 (en) 2012-05-30 2014-08-05 Biotix, Inc. Integrated pipette tip devices
EP3162442A4 (fr) * 2014-06-27 2018-01-03 A & D Company, Limited Embout de pipette
EP3115110B1 (fr) 2015-07-07 2019-03-27 Eppendorf Ag Pointe de pipette
CN105136515B (zh) * 2015-07-29 2017-09-29 俞雄飞 用于电镀线上镀液槽的取液管
CN109562372A (zh) * 2016-06-15 2019-04-02 汉密尔顿公司 移液装置、移液管末梢联接器和移液管末梢：装置和方法
CN107398310A (zh) * 2017-02-22 2017-11-28 佛山市工芯精密机械有限公司 一种移液器插嘴
CN114453042A (zh) * 2017-05-17 2022-05-10 拜欧迪克斯公司 人体工学移液管吸头
USD905865S1 (en) 2018-05-11 2020-12-22 Biotix, Inc. Pipette tip
JP6539774B1 (ja) * 2018-05-11 2019-07-03 バイオティクス， インコーポレイテッド ピペットチップ
EP3575000A1 (fr) 2018-05-31 2019-12-04 Eppendorf AG Pointe de pipette
WO2020131072A1 (fr) * 2018-12-20 2020-06-25 Tecan Trading Ag Extension de pointe de pipette, pointe de pipette, ensemble de pointe de pipette et extension de pointe de pipette, et procédé d'utilisation
EP3831485A1 (fr) 2019-12-03 2021-06-09 Eppendorf AG Pointe de pipette
EP3888791A1 (fr) * 2020-04-03 2021-10-06 Eppendorf AG Point de pipette
EP4063013A1 (fr) 2021-03-26 2022-09-28 Eppendorf AG Pointe de pipette

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE7518809U (de) * 1975-06-12 1978-09-14 Eppendorf Geraetebau Netheler & Hinz Gmbh, 2000 Hamburg Pipettenspitze
US5200151A (en) * 1990-05-21 1993-04-06 P B Diagnostic Systems, Inc. Fluid dispensing system having a pipette assembly with preset tip locator
US6197259B1 (en) 1998-11-06 2001-03-06 Rainin Instrument Co., Inc. Easy eject pipette tip
CN1179793C (zh) * 1998-11-06 2004-12-15 瑞宁器材公司 具有滴管尖端及安装轴的滴管
US7335337B1 (en) * 2001-09-11 2008-02-26 Smith James C Ergonomic pipette tip and adapters
DE10345324B3 (de) 2003-09-30 2004-08-19 Eppendorf Ag Pipettenspitze
US7641859B2 (en) * 2004-02-11 2010-01-05 Matrix Technologies Corporation Pipette tip mounting and ejection assembly and associated pipette tip
JP4521239B2 (ja) 2004-09-10 2010-08-11 株式会社日立ハイテクノロジーズ 磁場遮蔽装置及び生体磁場計測装置
EP2138234A1 (fr) * 2008-06-24 2009-12-30 F. Hoffmann-Roche AG Interface souple de pointe jetable

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
None *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4140588A1 (fr) 2021-08-30 2023-03-01 Tecan Trading AG Extrémité de pipette avec deux étapes d'angle positif

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CN103170386B (zh) 2016-10-26
JP2013136052A (ja) 2013-07-11
EP2606977A1 (fr) 2013-06-26
JP5775059B2 (ja) 2015-09-09
CN103170386A (zh) 2013-06-26

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