EP3888791A1 - Point de pipette - Google Patents

Point de pipette Download PDF

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Publication number
EP3888791A1
EP3888791A1 EP20167992.5A EP20167992A EP3888791A1 EP 3888791 A1 EP3888791 A1 EP 3888791A1 EP 20167992 A EP20167992 A EP 20167992A EP 3888791 A1 EP3888791 A1 EP 3888791A1
Authority
EP
European Patent Office
Prior art keywords
tubular body
pipette tip
flat
area
tip according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP20167992.5A
Other languages
German (de)
English (en)
Inventor
Hanna Rethwisch
Maren LEONHARDT
Laura Koch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Priority to EP20167992.5A priority Critical patent/EP3888791A1/fr
Priority to CN202180025758.9A priority patent/CN115397557A/zh
Priority to US17/912,958 priority patent/US20230138840A1/en
Priority to PCT/EP2021/057967 priority patent/WO2021198086A1/fr
Priority to JP2022557723A priority patent/JP2023520982A/ja
Priority to EP21714190.2A priority patent/EP4100164A1/fr
Publication of EP3888791A1 publication Critical patent/EP3888791A1/fr
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/08—Ergonomic or safety aspects of handling devices
    • B01L2200/087—Ergonomic aspects
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric

Definitions

  • the invention relates to a pipette tip.
  • Pipette tips are used together with pipettes and other dosing devices in particular in medical, biological, biochemical and chemical laboratories for dosing liquids.
  • pipettes and other metering devices are collectively referred to as "pipetting devices”.
  • Pipette tips have an elongated, tubular body which at the lower end has a lower opening for the passage of liquid and at the upper end an upper opening for clamping onto the neck of a pipetting device.
  • Pipette tips usually have a generally conical shape, the cross section of which increases from the lower opening to the upper opening.
  • Standardized conical or frustoconical attachments with a standard geometry which is used uniformly by many manufacturers and which is characterized for each pipette tip size by a specific mean diameter and by a specific cone angle of the conical attachment are known.
  • Multi-channel pipetting devices are used to simultaneously take up liquid from one or more vessels or to dispense it into one or more vessels. They are often used for processing microtiter plates that have a large number of vessels in a matrix-like arrangement.
  • multichannel pipetting devices have a plurality of conical attachments which are arranged in parallel next to one another in one or more parallel rows and onto which the pipette tips can be clamped.
  • multichannel pipettes with 8, 12, 16 or 24 attachments in a row are known.
  • the pipetting device When designed as an air-cushion pipetting device, the pipetting device has at least one displacement device for air, which is connected in a communicating manner to a through hole of at least one attachment.
  • An air cushion can be displaced by means of the displacement device in order to suck liquid into a pipette tip clamped onto the attachment and to expel it therefrom.
  • the displacement device is usually designed as a cylinder with a piston that can be displaced therein.
  • displacement devices with a displacement chamber and at least one deformable wall are also known, a deformation of the wall causing the displacement of the air cushion.
  • a small piston is arranged in the pipette tip, which is coupled to a coupling element of a piston drive of the pipetting device when the pipette tip is plugged onto an attachment, which can be displaced in a through hole of the attachment.
  • the liquid is absorbed into the pipette tip preferably in a single step or in several small steps.
  • the liquid is dispensed in a single step when pipetting and in several small steps when dispensing.
  • Pipetting devices usually have an ejector that acts on the upper edge of the pipette tip in order to push it away from the attachment.
  • the ejector can be pressed against the upper edges of several pipette tips at the same time.
  • the pipetting device can be a hand-held pipette which the user can hold and operate with only one hand. It can also be a dispensing station ("pipetting station”) or an automatic dispensing machine (“pipetting machine”) in which a dispensing head with one or more attachments on a robot arm or on another transmission system above a work surface can be displaced.
  • the pipetting device can also be part of a laboratory machine ("workstation”) which, in addition to dosing, can carry out further treatments of liquids (eg mixing, temperature control, analysis).
  • the pipette tip To avoid incorrect dosing, the pipette tip must be clamped tightly or sealingly onto the attachment. In addition, the forces for attaching and ejecting the pipette tip from the attachment must not be too high.
  • Conventional pipette tips are thick-walled and rigid in the contact area with the conical attachment. When plugging on, the pipette tips are elastically widened at the circumference by the attachment. The spring characteristic is steep, so that high slip-on forces have to be applied. After attaching, there is a correspondingly high static friction between the attachment and the pipette tip, which must be overcome when it is ejected. The user is stressed by the high forces involved in attaching and ejecting the pipette tip. This can trigger diseases that are summarized under the term "cumulative trauma disorders" (CTD). As far as the attachment and ejection takes place by means of motorized drives, these must be correspondingly powerful and have a high power consumption.
  • CTD cumulative trauma disorders
  • the U.S. 6,197,259 describes a pipette tip which, by applying relatively low axial push-on forces of six pounds (26.7 N), can be securely attached to a neck of a pipette and by using relatively low ejection forces of three pounds (13.3 N) can be released from this.
  • the pipette tip has a conical upper end with an inner diameter at the upper end which is larger than the diameter of the attachment of the pipette onto which the pipette tip is to be attached.
  • the pipette tip has a hollow central section and an annular sealing area at the connection between the upper end and the central section.
  • the middle section has a side wall with a wall thickness between 0.2 and 0.5 mm on and next to the sealing area.
  • the annular sealing area has an inner diameter smaller than a value "x" and is designed to engage the lower end of a sealing zone of the lug to be expanded radially when the lug is inserted. This creates a liquid-tight seal between the sealing zone of the attachment and the sealing area of the pipette tip.
  • the pipette tip has lateral stabilizing means on the inside, next to the sealing area, which come into engagement with the outer surface of the attachment in order to stabilize the pipette tip on the attachment.
  • the lateral stabilizing means have at least three contacts spaced from one another in the circumferential direction and extending inward from the inner surface of the pipette tip.
  • the diametrical spacing of the contacts is such that they easily engage the lower end of the hub and allow the lower end to slide past without widening the sidewalls of the pipette tip on which the contacts are arranged.
  • the pipette tip is stretched in the sealing area and immediately next to it.
  • the contacts guide the pipette tip on the attachment the side wall of the pipette tip is deformed inward between the contacts and is not expanded, as a result of which the force that has to be applied to push in the attachment is kept low.
  • the attachment can be pressed deeper into the pipette tip as the push-on force increases. Correspondingly high ejection forces are required to loosen the pipette tip from the Approach. Due to the inward protruding contacts, the construction is only suitable for relatively large pipette tips.
  • the U.S. 6,568,288 describes a pipette tip that has axially spaced apart annular sealing and essentially cylindrical lateral guide areas, the sealing area being sufficiently thin to provide a press fit and airtight seal when a pipette attachment with axially spaced apart annular sealing and cylindrical lateral guide zones penetrates to form a sealing surface of the sealing zone and the sealing area.
  • the wall thickness in the sealing area is preferably between 0.2 and 0.5 mm.
  • the sealing surface is the outer surface of an annular, radially outwardly projecting projection next to the lower end of the extension.
  • the pipette tip has a circular, upward-facing and inward-facing shoulder on the inner circumference in order to limit attachment to the attachment.
  • the pushing force should be about two pounds (8.9 N) and about one pound (4.45 N) to throw off.
  • the depth stop can result in incomplete immersion when several pipette tips are picked up from a tray or rack at the same time using a multi-channel pipetting device. If the tray or rack bends slightly downwards between the side edges, placing the two outer attachments on the shoulders of the two outer pipette tips can lead to insufficient immersion of the other attachments in the pipette tips arranged between them.
  • the US 6,967,004 B2 describes a pipette tip which has an annular sealing area with an inner sealing surface on a side wall which is sufficiently thin in the sealing area to expand slightly and a press fit and an airtight seal between the sealing surface and a sealing zone to form a neck of the pipette inserted into the pipette tip.
  • the pipette tip has a circular, inwardly and upwardly directed shoulder that limits the insertion of the attachment.
  • the approach has two cylinder sections with different diameters. Its annular sealing zone includes a sealing edge at a connection between the lower end of a cylinder section and the outermost edge of a radially extending transition of the extension.
  • the forces to insert and eject the pipette tip are less than two pounds (8.9 N). If several pipette tips are picked up at the same time by means of a multi-channel pipetting device, the depth stop can lead to insufficient immersion of approaches.
  • the EP 2 138 234 A1 describes a pipette tip which, for releasable connection with the attachment of a pipetting device, has a flexible, tubular connecting section with a contour that is wave-shaped in cross section at the upper end of an elongated tubular section, which increases the expandability of the seat area.
  • the seating area is reversibly expandable by more than 20% when it is attached to the attachment.
  • the wave-shaped contour must be drawn smooth on the approach, so that the further elasticity is only low. As a result, the pipette tip requires precise manufacture.
  • the EP 2 606 977 A1 describes a pipette tip with the shape of an elongated tube with a lower opening at the lower end for the passage of liquid and an upper opening at the upper end, wherein next to the upper opening on the inner circumference there is a seat area which can be pushed on a standardized conical approach of a pipetting device is used.
  • the seat area has a holding area with radially inwardly protruding, axially extending ribs and below the holding area a sealing area with a circumferential, inwardly protruding sealing projection.
  • the seat area is designed in such a way that the ribs are partially plastically deformed when plugged onto the attachment with a push-on force that ensures that the pipette tip is held and sealed on the attachment and an elastic deformation occurs outside the ribs in the seat area.
  • Below the sealing area it has a braking area that widens conically towards the upper opening to limit attachment. This ensures a secure seal on the attachment of a pipetting device and significantly reduces the ejection force to be applied for ejection.
  • the design is particularly suitable for relatively large pipette tips with a nominal volume of 2.5, 5.0 and 10 mL. It is less suitable for smaller pipette tips due to the difficult production of the filigree ribs.
  • the EP 3 115 110 A1 describes a pipette tip with a tubular body and a seat area for attaching to a conical attachment of a pipetting device, which has a circumferential, inwardly protruding sealing projection on the inner circumference at a distance from the upper opening, below the sealing projection a circumferential, downwardly stronger than the Approach tapering braking area and above the sealing projection has a circumferential, inwardly protruding support projection.
  • the sealing projection can be clamped onto the projection in a sealing manner with elastic deformation, the braking area resting further down on the projection and the supporting projection resting above the projection without bias or being spaced from the projection by a circumferential gap.
  • the pipette tip seals well and can be securely clamped onto the attachment of a pipetting device, can be ejected from the attachment with reduced expenditure of force and is also well suited for smaller pipette tip sizes.
  • the disadvantage is that still high effort when clamping on the approach and when stripping from the approach.
  • the WO 2011/091308 A2 describes a pipette tip which has an annular flange at the proximal end of a proximal section and axially directed ribs in the proximal section which are spaced apart from one another in the circumferential direction.
  • the flange is intended to increase the rigidity of the pipette tip and to make it easier to align the dispenser with the pipette tip.
  • the ribs are intended to limit the axial expandability of the pipette tip in the proximal area.
  • the push-on forces of the pipette tips with 200 ⁇ l and 1000 ⁇ l filling volumes on five different pipettes are over 1000 g (10 N) and reach up to 2,000 g (20N).
  • the pipette tip has elastic expansion elements by means of which the axial push-on force and ejection force are reduced.
  • the elastic expansion elements are arranged in an upper section of the pipette tip above a sealing ring encircling the inner circumference. They are formed by outwardly curved areas of reduced wall thickness between cylindrical or conical segments of the pipette tip.
  • the pipette tip is guided and aligned on the attachment by ribs on the inside of the wall segments.
  • the push-on forces are still high because the pipette tip has a large wall thickness in the area of the circumferential sealing ring and can only expand slightly, and they increase sharply when the expansion elements are drawn flat.
  • the WO 2018/213196 A1 describes a pipette tip having a proximal portion with alternating, longitudinally directed grooves and panels which are designed to facilitate the expansion and compression of the wall when the pipette tip is attached to a correspondingly designed dispensing device and is sealingly engaged therewith. These measures reduce the axial force for clamping a pipette tip onto a dispensing device and releasing a pipette tip from a dispensing device for liquid.
  • the grooves are stepped, V- or U-shaped.
  • a plurality of grooves and panels are arranged alternately on the circumference of the proximal portion. To form the grooves, constrictions in the injection mold must be filled with plasticized plastic compound during injection molding.
  • the invention is based on the object of providing a pipette tip which can be clamped onto an attachment of a pipetting device in a sufficiently firm and sealing manner with reduced attachment force and ejection force, has more favorable manufacturing properties with improved dimensional accuracy and strength and is better suited for identifying different types of pipette tips .
  • the plastic pipette tip according to the invention comprises an elongated, tubular body with a lower opening at the lower end for the passage of liquid and an upper opening at the upper end for clamping onto a neck of a pipetting device, in addition to the upper opening on the inner circumference of the tubular Body there is a seating area for the approach, characterized in that in addition to the upper opening on the outer circumference of the tubular body there is at least one flattening extending in the axial direction, the wall thickness of the tubular body in a cross section through the tubular body starting from one of the two areas of the tubular body adjoining the flattening gradually decreased in the flattening towards its central area and the flattening in the cross section through the tubular body is a straight line or less strongly curved than the areas adjoining the flattening P rofil has.
  • the pipette tip according to the invention has a smaller wall thickness in the area of the flattened area than in the areas of the tubular body adjoining the flattened area. This improves the deformability of the pipette tip when it is clamped onto an attachment of a pipetting device, so that reliable sealing of the pipette tip on the attachment can be achieved even with comparatively low push-on forces.
  • the pipette tip can be designed in such a way that it is exclusively elastically deformed when it is clamped with a certain force on a defined attachment of a pipetting device. However, it can also be designed in such a way that it is plastically deformed when it is clamped with a certain force on a defined attachment of a pipetting device.
  • the push-on force increases proportionally to the deformation.
  • the elastic deformation can be completely restored after the pipette tip has been released from the attachment.
  • plastic deformation occurs.
  • Plastic deformation is irreversible Deformation that does not resolve itself after the pipette tip is detached from the attachment.
  • the push-on force no longer increases or only increases slightly with the deformation.
  • the elastic or plastic deformation takes place preferably in the area of the flattened areas, since the pipette tip has the smallest wall thickness in addition to the upper opening in the area of the flattened area. As a result, the effort required to clamp the pipette tip onto the attachment in a sealing manner can be kept low.
  • the push-on force can be limited to a specified limit value.
  • the plastic compound can better fill the cavity in the injection molding tool in the area of the flattened area than in the area of the grooves in the conventional pipette tips due to the lower pressure losses at the flattened area.
  • weld lines can also be reduced and more dimensionally stable and stronger pipette tips can be achieved.
  • the increased strength can prevent the pipette tips from tearing open at the points with the smallest wall thickness due to the push-on forces.
  • the flattened area can be used to identify the pipette tips.
  • information about the pipette tip and / or its manufacture can be affixed to the flattened area during injection molding, for example about the pipette tip size, the material or the degree of purity of the pipette tip, the manufacturer, the brand and / or the production tool used for the manufacture.
  • the pipette tip size is the largest volume that can be dosed with the pipette tip.
  • Pipette tips which differ from one another by at least one of the aforementioned criteria are also referred to in this application as “pipette tips of different pipette tip types”.
  • the marking can be used at Injection molding in the form of raised or recessed letters, numbers, characters or symbols or printed later. Furthermore, identification by the user comes into consideration, for example by printing, writing using a writing tool or sticking a label. In addition, the flattening itself can be used as a distinguishing feature in order to distinguish different types of pipette tips from one another.
  • the flattened area can also serve as anti-roll protection in order to prevent a pipette tip placed on a work surface or other surface from rolling away.
  • the flattened area can have a straight profile and / or an outwardly (convex) and / or inwardly (concave) profile.
  • the profile of the flattening is largely or exclusively straight or largely or exclusively curved outward or largely or exclusively curved inward.
  • the radius of curvature is constant along the respective profile.
  • the radius of curvature varies along the respective profile or part thereof.
  • the profile of the flattened portion is rectilinear in sections and the same or differently curved in sections.
  • the profile of the flattening is straight on the two edges and curved inward between them, so that it is approximately V-shaped overall.
  • the profile of the flattening is curved differently in sections.
  • the different curvatures can be curvatures with different radii of curvature or outward and inward curvatures with the same or different radii of curvature. If the profile of the flat is curved, the curvature is less than the curvature of that on it adjacent areas of the tubular body.
  • the curvature is the reciprocal of the radius of curvature of the respective profile. In the case of an outwardly curved profile, the flattening results from the fact that its curvature is smaller than the curvature of the areas of the tubular body adjoining it.
  • the flattening also results from the fact that the curvature is less than the curvature of the region of the tubular body adjoining it.
  • the flattened area is particularly suitable for identifying pipette tips.
  • the tubular body has, in a cross section on the outer circumference, at least in the areas adjoining the flattened area, an arcuate profile and the flattened area has a straight or an arcuate profile with a greater radius of curvature than the areas adjoining the flattened area.
  • the areas adjoining the flattened area are overall in the shape of a circular arc.
  • the invention also includes embodiments in which the profile of the areas adjoining the flat area deviates from a circular arc shape, for example has an elliptical or otherwise curved course.
  • the profile of the flattening can deviate from a straight or circular arc-shaped curved course, for example have an elliptical or otherwise curved course.
  • the tubular body has a straight and / or a curved profile in all cross sections through the flattening in the flattening.
  • the tubular body has exclusively a straight cross-section in all cross-sections through the flattening in the flattening or in all cross-sections the flat only has a curved profile in the flat.
  • the tubular body has in all cross-sections through the flattening in the flattening exclusively a profile curved with the same radius of curvature or in different cross-sections a profile curved with a different radius of curvature, the curvature of the profile preferably changing gradually from cross-section to cross-section.
  • the flattening has an outwardly curved profile and an inwardly curved profile in different cross-sections, the curvature of the profile preferably changing gradually from cross-section to cross-section.
  • the flattened area has an inwardly curved profile.
  • this is a profile that is inwardly curved overall with the same or different curvatures or a profile that is curved inward in the central area and a straight profile on the two lateral edges that is approximately V-shaped overall.
  • the inwardly curved flattening is preferably in a region on the circumference of more than 10 °, preferably more than 30 °.
  • at least 3 flat areas with an inwardly curved contour are evenly distributed over the circumference and the flat areas with an inwardly curved contour are the same width or wider than the areas between the flat areas with an inwardly curved contour.
  • the at least one flat area extends upwards to a distance from the upper end of the tubular body.
  • an upper edge with a uniform wall thickness can be achieved, which is advantageous for ejecting a pipette tip from the attachment by means of an ejection device of the pipetting device.
  • the upper edge of the pipette tip is a circumferential flange. The flange can be used to hold a pipette tip in a hole of a holder for pipette tips ( rack ).
  • the flat extends to the upper end of the tubular body.
  • the extension of the flattened area up to the upper end is advantageous for the deformation of the pipette tip with reduced expenditure of force.
  • the tubular body has a shoulder on the outer circumference. With the shoulder, the pipette tip can be supported in a hole in a holder for pipette tips.
  • the flattening extends downwards at least up to the shoulder or beyond.
  • the tubular body has several, preferably three, flat areas on the circumference.
  • the force required to deform the pipette tips can be further reduced by the multiple flattened areas.
  • different types of pipette tips can be identified differently by pipette tips with different numbers and / or positions and / or dimensions of the flattened areas.
  • the flats are evenly distributed over the outer circumference of the tubular body.
  • a uniform expansion of the pipette tip can hereby be achieved.
  • the flattened areas can, however, also be distributed unevenly over the outer circumference of the tubular body, in particular in order to identify different types of pipette tips differently.
  • the flattened areas as a whole take up a larger part of the outer circumference of the tubular body than the regions of the tubular body adjoining them in the circumferential direction as a whole. As a result, the effort required to expand the pipette tip can be further reduced.
  • the seat area is conical and / or cylindrical.
  • the tubular body on the inner circumference of the seat area has at least one inwardly protruding, circumferential sealing structure and / or at least one inwardly protruding, circumferential or multiple spaced-apart guide structure.
  • the sealing structure is a sealing bead and / or the guide structure is a guide bead and / or a guide rib. The sealing and / or guide structures cause a ring support between the attachment of the pipetting device and the pipette tip and thus very low frictional forces when the pipette tip is attached to the attachment.
  • the tubular body has a plurality of sealing structures and / or guide structures on the inner circumference, which have a wave-shaped contour in a longitudinal section through the tubular body.
  • the tubular body has a widening at the upper opening and / or an insertion bevel on the inner circumference.
  • the widening and / or insertion bevel facilitates the insertion of a tip of a pipetting device into the pipette tip.
  • the pipette tip is made from at least one thermoplastic, preferably from at least one polyolefin, preferably from at least one polypropylene and / or polyethylene.
  • the invention also relates to a pipette tip system comprising several pipette tips according to one of claims 1 to 15 or one of the preceding embodiments, pipette tips of different pipette tip types having differently designed flats and / or different markings on the flats.
  • the invention also relates to a pipetting system with at least one pipette tip according to one of claims 1 to 15 or one of the preceding embodiments and a single-channel pipetting device with a single attachment for attaching a pipette tip and / or a multi-channel pipetting device with several attachments for simultaneously attaching several pipette tips.
  • each cross-section through the tubular body is a plane oriented perpendicular to the central axis of the tubular body.
  • Each longitudinal section through the tubular body is a plane in which the central axis of the tubular body extends.
  • the central area of the flat is a line or a strip-shaped zone which runs between the two side edges of the flat, wherein the line or zone can have the same distances from the two side edges of the flat or different distances from the two side edges of the May have flattening.
  • a pipette tip 1 has an elongated, tubular body 2 which has a lower opening 4 at the lower end 3 and an upper opening 6 at the upper end 5.
  • the lower opening 4 is smaller than the upper opening 6.
  • the inner and outer diameter of the tubular body 2 increases from the lower opening 4 to the upper opening 6.
  • the tubular body 2 has a conical starting section 7 at the bottom, above a middle section 8 with a smaller cone angle than the starting section 7 and above a cylindrical head section 9 with larger dimensions than the middle section 8 outer circumference of the tubular body 2 a downwardly directed outer shoulder 10 around.
  • Flattened areas 12 extending in the axial direction are provided on the outer circumference 11 of the head section.
  • the flats 12 begin at a small distance from the upper end 5 of the tubular body 2 and extend downward over the head section 9 to a little over the shoulder 10 and into the upper area of the central section 8.
  • the flats 12 are aligned parallel to the central axis of the tubular body 2.
  • the flats 12 are arranged distributed uniformly around the outer circumference 11 of the tubular body.
  • the flattened areas 12 are each approximately the same width as the areas 13 adjoining them.
  • the tubular body 2 has in each cross section through the flats 12 on the outer circumference 11 in the areas 13 adjoining the flats 12 an arcuate profile 14 and in the flats 12 a straight profile 15.
  • the arcuate profiles 14 extend over the entire distance range between two adjacent flattened areas 12, that is to say between the flattened areas 12, the profiles 14 are arc-shaped everywhere. This is especially true in Fig. 3 shown.
  • the tubular body 2 has a circumferential edge 16 with a uniform wall thickness.
  • the tubular body 2 has a widening 18 with an insertion bevel 19 on the inner circumference 17. This is especially true in Fig. 4 shown.
  • the tubular body 2 has on the inner circumference 17 a substantially conical or cylindrical seat area 20 for a conical or cylindrical extension 21 of a pipetting device.
  • the seat area 20 extends into the head section 9 and is conical with a cone angle of, for example, 2 ° to 6 °.
  • the tubular body 2 has on the inner circumference 17 in the seat area 20 a plurality of sealing structures 22 in the form of inwardly projecting, closed circumferential sealing beads 23 which are spaced apart from one another in the axial direction. Furthermore, the tubular body 2 has, at a smaller distance from the upper opening 6, inwardly projecting, closed circumferential guide structures 24 in the form of guide beads 25 which are spaced apart from one another. Overall, the tubular body 2 in the seat area 20 has wave-shaped sealing and guide structures 22, 24 in longitudinal section.
  • the tubular body 2 has a constant wall thickness everywhere in cross section in the adjoining areas 13 between the flattened areas 12. Furthermore, the wall thickness of the tubular body 2 in the flattened areas 12 decreases in each case starting from the areas 13 adjoining them towards the central area 27 of the flattened areas 12.
  • the flats 12 are provided with markings 28. These are inscriptions 29 in the form of raised or recessed numbers and letters that are produced during the injection molding of the pipette tip 1.
  • the inscriptions 29 indicate the size of the pipette tip and the nest of the injection molding tool in which the pipette tip 1 is injection molded.
  • one or more pipette tips 1 can be kept ready in holes of a holder for pipette tips, with their shoulder 10 being supported on the edge of the holes.
  • the extension 21 of a pipetting device can easily be introduced into the pipette tip 1 through the widening 18 with the insertion bevel 19 at the upper opening 6. In it, it is guided with little friction by the guide structures 24 and sealing structures 22.
  • the pipette tip 1 can be elastically and / or plastically deformed in the area of the flattened areas 12, whereby the attachment forces are reduced and a secure seal of the attachment 21 on the sealing structures 22 is achieved with relatively lower attachment forces.
  • the pipette tip 1 After the pipetting of liquid, the pipette tip 1 can easily be thrown off the attachment 21, since the ejection forces to be applied for ejection are also reduced.
  • a drop sleeve guided on the approach 21 is the The pipetting device is pressed against the circumferential edge 16 at the upper end 5 of the pipette tip 1 and the pipette tip is stripped off the attachment 21.
  • the pipette tip 1 of Figures 6 to 10 is preferably for pipetting smaller filling volumes than the pipette tip 1 of Figs. 1 to 5 designed.
  • the pipette tip 1 differs from the one described above in particular in that the elongated, tubular body 2 has a plurality of conical middle sections 8.1, 8.2, 8.3 above the conical starting section 7, a conical transition section 30 above and a conical head section 9 with a circumferential, radially following has externally projecting flange 31 at the upper end 5.
  • the aforementioned sections 7, 8.1, 8.2, 8.3, 40 and the flange 31 directly adjoin one another.
  • the outer diameter of the tubular body 2 increases gradually from the lower end 3 to the upper end of the head section 9.
  • the inner diameter of the tubular body 2 also increases gradually from the lower end 3 to the upper end 5 of the tubular body 2.
  • This pipette tip 1 has no shoulder between the central section and the head section.
  • the flange 31 has on the underside downwardly projecting ribs 32 which, starting from the head section 9, extend radially outward.
  • the flattened areas 12 extend from the underside of the flange 31 in the axial direction of the tubular body 2 to the upper edge area of the transition section 30.
  • This pipette tip 1 also has three flat flat areas 12.
  • the adjoining areas 13 of the head section 9 each have an arcuate profile in cross section, which extends over the entire area between two adjacent flattened areas 12.
  • One or more pipette tips 1 according to Figures 6 to 10 can be provided in a holder for pipette tips. They are inserted into holes in the holder and supported with the ribs 32 on the underside of the flange 31 on the edge of the holes. According to Fig. 10 the extension 21 is only partially pushed into the top guide bead 25 in the pipette tip 1. Further down, the pipette tip 1 has a further guide bead 25 and below it a sealing bead 23. The attachment is pushed forward up to the sealing bead 23 so that the pipette tip 1 is aligned and clamped onto the attachment 21 in a sealing manner. The guide beads 25 also contribute to the sealing of the pipette tip 1 on the attachment 21.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)
EP20167992.5A 2020-04-03 2020-04-03 Point de pipette Withdrawn EP3888791A1 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP20167992.5A EP3888791A1 (fr) 2020-04-03 2020-04-03 Point de pipette
CN202180025758.9A CN115397557A (zh) 2020-04-03 2021-03-26 移液器吸头
US17/912,958 US20230138840A1 (en) 2020-04-03 2021-03-26 Pipette Tip
PCT/EP2021/057967 WO2021198086A1 (fr) 2020-04-03 2021-03-26 Pointe de pipette
JP2022557723A JP2023520982A (ja) 2020-04-03 2021-03-26 ピペットチップ
EP21714190.2A EP4100164A1 (fr) 2020-04-03 2021-03-26 Pointe de pipette

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP20167992.5A EP3888791A1 (fr) 2020-04-03 2020-04-03 Point de pipette

Publications (1)

Publication Number Publication Date
EP3888791A1 true EP3888791A1 (fr) 2021-10-06

Family

ID=70189716

Family Applications (2)

Application Number Title Priority Date Filing Date
EP20167992.5A Withdrawn EP3888791A1 (fr) 2020-04-03 2020-04-03 Point de pipette
EP21714190.2A Pending EP4100164A1 (fr) 2020-04-03 2021-03-26 Pointe de pipette

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP21714190.2A Pending EP4100164A1 (fr) 2020-04-03 2021-03-26 Pointe de pipette

Country Status (5)

Country Link
US (1) US20230138840A1 (fr)
EP (2) EP3888791A1 (fr)
JP (1) JP2023520982A (fr)
CN (1) CN115397557A (fr)
WO (1) WO2021198086A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102022120212A1 (de) 2022-08-10 2024-02-15 Hamilton Bonaduz Ag Pipettierspitze mit sich gekrümmt verjüngendem Aufnahmeraum

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6197259B1 (en) 1998-11-06 2001-03-06 Rainin Instrument Co., Inc. Easy eject pipette tip
US6568288B2 (en) 2001-06-29 2003-05-27 Rainin Instrument, Llc Pipette with improved pipette tip and mounting shaft
US6967004B2 (en) 2001-10-30 2005-11-22 Rainin Instrument, Llc Pipette with improved pipette tip and mounting shaft
US7335337B1 (en) 2001-09-11 2008-02-26 Smith James C Ergonomic pipette tip and adapters
US20090317303A1 (en) * 2008-06-24 2009-12-24 Roche Molecular Systems, Inc. Flexible disposable tip interface
WO2011091308A2 (fr) 2010-01-22 2011-07-28 Biotix, Inc. Pointes de pipette
EP2606977A1 (fr) 2011-12-23 2013-06-26 Eppendorf AG Pointe de pipette
EP3115110A1 (fr) 2015-07-07 2017-01-11 Eppendorf Ag Pointe de pipette
WO2018213196A1 (fr) 2017-05-17 2018-11-22 Biotix, Inc. Embouts de pipette ergonomiques
US20190344258A1 (en) * 2018-05-11 2019-11-14 Biotix, Inc. Pipette tip

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6197259B1 (en) 1998-11-06 2001-03-06 Rainin Instrument Co., Inc. Easy eject pipette tip
US6568288B2 (en) 2001-06-29 2003-05-27 Rainin Instrument, Llc Pipette with improved pipette tip and mounting shaft
US7335337B1 (en) 2001-09-11 2008-02-26 Smith James C Ergonomic pipette tip and adapters
US6967004B2 (en) 2001-10-30 2005-11-22 Rainin Instrument, Llc Pipette with improved pipette tip and mounting shaft
US20090317303A1 (en) * 2008-06-24 2009-12-24 Roche Molecular Systems, Inc. Flexible disposable tip interface
EP2138234A1 (fr) 2008-06-24 2009-12-30 F. Hoffmann-Roche AG Interface souple de pointe jetable
WO2011091308A2 (fr) 2010-01-22 2011-07-28 Biotix, Inc. Pointes de pipette
EP2606977A1 (fr) 2011-12-23 2013-06-26 Eppendorf AG Pointe de pipette
JP2013136052A (ja) * 2011-12-23 2013-07-11 Eppendorf Ag ピペットチップ
EP3115110A1 (fr) 2015-07-07 2017-01-11 Eppendorf Ag Pointe de pipette
WO2018213196A1 (fr) 2017-05-17 2018-11-22 Biotix, Inc. Embouts de pipette ergonomiques
CN110944751A (zh) * 2017-05-17 2020-03-31 拜欧迪克斯公司 人体工学移液管吸头
US20190344258A1 (en) * 2018-05-11 2019-11-14 Biotix, Inc. Pipette tip

Also Published As

Publication number Publication date
WO2021198086A1 (fr) 2021-10-07
EP4100164A1 (fr) 2022-12-14
US20230138840A1 (en) 2023-05-04
CN115397557A (zh) 2022-11-25
JP2023520982A (ja) 2023-05-23

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