EP3765846C0 - Verfahren zur verbesserung der lebensfähigkeit von zellen in einem produktionsbioreaktor - Google Patents

Verfahren zur verbesserung der lebensfähigkeit von zellen in einem produktionsbioreaktor

Info

Publication number
EP3765846C0
EP3765846C0 EP19714056.9A EP19714056A EP3765846C0 EP 3765846 C0 EP3765846 C0 EP 3765846C0 EP 19714056 A EP19714056 A EP 19714056A EP 3765846 C0 EP3765846 C0 EP 3765846C0
Authority
EP
European Patent Office
Prior art keywords
cell viability
production bioreactor
improving cell
improving
bioreactor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP19714056.9A
Other languages
English (en)
French (fr)
Other versions
EP3765846B1 (de
EP3765846A1 (de
Inventor
Jonathan Wang
Neha Shah
Jason Walther
Jiuyi Lu
Timothy Johnson
Yukun Ren
Jean Mclarty
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genzyme Corp
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Priority to EP24209426.6A priority Critical patent/EP4512887A3/de
Publication of EP3765846A1 publication Critical patent/EP3765846A1/de
Application granted granted Critical
Publication of EP3765846C0 publication Critical patent/EP3765846C0/de
Publication of EP3765846B1 publication Critical patent/EP3765846B1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18—Flow directing inserts
    • C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP19714056.9A 2018-03-16 2019-03-15 Verfahren zur verbesserung der lebensfähigkeit von zellen in einem produktionsbioreaktor Active EP3765846B1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP24209426.6A EP4512887A3 (de) 2018-03-16 2019-03-15 Verfahren zur verbesserung der lebensfähigkeit von zellen in einem produktionsbioreaktor

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862644339P 2018-03-16 2018-03-16
US201862645755P 2018-03-20 2018-03-20
PCT/US2019/022530 WO2019178508A1 (en) 2018-03-16 2019-03-15 Methods for improving cell viability in a production bioreactor

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP24209426.6A Division EP4512887A3 (de) 2018-03-16 2019-03-15 Verfahren zur verbesserung der lebensfähigkeit von zellen in einem produktionsbioreaktor

Publications (3)

Publication Number Publication Date
EP3765846A1 EP3765846A1 (de) 2021-01-20
EP3765846C0 true EP3765846C0 (de) 2024-10-30
EP3765846B1 EP3765846B1 (de) 2024-10-30

Family

ID=65952204

Family Applications (2)

Application Number Title Priority Date Filing Date
EP19714056.9A Active EP3765846B1 (de) 2018-03-16 2019-03-15 Verfahren zur verbesserung der lebensfähigkeit von zellen in einem produktionsbioreaktor
EP24209426.6A Pending EP4512887A3 (de) 2018-03-16 2019-03-15 Verfahren zur verbesserung der lebensfähigkeit von zellen in einem produktionsbioreaktor

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP24209426.6A Pending EP4512887A3 (de) 2018-03-16 2019-03-15 Verfahren zur verbesserung der lebensfähigkeit von zellen in einem produktionsbioreaktor

Country Status (18)

Country Link
US (2) US11391725B2 (de)
EP (2) EP3765846B1 (de)
JP (2) JP2021515584A (de)
KR (2) KR20250016476A (de)
CN (2) CN112136045B (de)
AU (1) AU2019236246B2 (de)
BR (1) BR112020018823A2 (de)
CA (1) CA3094076A1 (de)
ES (1) ES3009097T3 (de)
HU (1) HUE069711T2 (de)
IL (2) IL277352B2 (de)
MA (1) MA52007A (de)
MX (2) MX2020009605A (de)
PL (1) PL3765846T3 (de)
SG (1) SG11202009047TA (de)
TW (2) TW202513790A (de)
WO (1) WO2019178508A1 (de)
ZA (2) ZA202005741B (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4410957A1 (de) 2021-09-30 2024-08-07 FUJIFILM Corporation Zellkulturverfahren und herstellungsverfahren für nützliche substanzen
US12157873B2 (en) 2022-09-28 2024-12-03 Genzyme Corporation Systems and devices for producing biological products and methods of using the same
US20240263122A1 (en) * 2023-02-08 2024-08-08 National Central University Bioreactor apparatus, ion-exchange substrate and method for regulation of ion concentration and cell culture thereof

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5563068A (en) * 1994-04-21 1996-10-08 Genetic Therapy, Inc. Bioreactor
US20080145364A1 (en) * 2005-02-15 2008-06-19 Yale University Method for High Throughput Screening for Anitbodies and Proteins Inducing Apoptosis
EP2500415A1 (de) * 2006-09-13 2012-09-19 Abbott Laboratories Verbesserungen an Zellkulturen
MX345141B (es) * 2006-09-13 2017-01-18 Abbvie Inc * Mejoras de cultivos celulares.
RU2468076C2 (ru) * 2007-05-07 2012-11-27 Проталикс Лтд. Одноразовое устройство для культивирования и сбора растительной ткани и/или клеток, способ и система культивирования и сбора растительной ткани и/или растительных клеток
US8728479B2 (en) 2009-03-31 2014-05-20 The Trustees Of The University Of Pennsylvania Antigen-binding proteins comprising recombinant protein scaffolds
US8313926B2 (en) * 2009-07-31 2012-11-20 Baxter International Inc. Methods for expressing ADAMTS proteins in cell culture medium supplemented with zinc
WO2014110433A1 (en) * 2013-01-10 2014-07-17 Biogen Idec Ma Inc. Medium supplements for improved process performance
MX367025B (es) * 2013-02-22 2019-08-02 Genzyme Corp Metodos de cultivo por perfusion con microportadores y usos de los mismos.
US20140271633A1 (en) * 2013-03-14 2014-09-18 Abbvie Inc. Mammalian cell culture performance through surfactant supplementation of feed media
SG11201506034TA (en) * 2013-03-15 2015-09-29 Alder Biopharmaceuticals Inc Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
EP3003325A4 (de) * 2013-05-29 2017-01-11 Biogen MA Inc. Verfahren zur bewertung von zellkultur-zusatzstoffen
CN106133146B (zh) * 2014-02-27 2021-05-04 豪夫迈·罗氏有限公司 重组糖蛋白生产中的细胞生长和糖基化的调节
KR102769649B1 (ko) 2014-12-22 2025-02-19 젠자임 코포레이션 포유류 세포의 배양 방법
TWI541353B (zh) * 2014-12-25 2016-07-11 財團法人工業技術研究院 產生嗜熱性鹼性蛋白酶生產菌屬（Ｔｅｐｉｄｉｍｏｎａｓ）之胞外蛋白的方法
MX2018002121A (es) * 2015-08-17 2018-06-18 Alexion Pharma Inc Elaboracion de fosfatasas alcalinas.

Also Published As

Publication number Publication date
CA3094076A1 (en) 2019-09-19
KR20250016476A (ko) 2025-02-03
MA52007A (fr) 2021-01-20
JP2024023489A (ja) 2024-02-21
TW202513790A (zh) 2025-04-01
SG11202009047TA (en) 2020-10-29
IL319295A (en) 2025-04-01
CN112136045B (zh) 2024-12-13
US11391725B2 (en) 2022-07-19
KR102757905B1 (ko) 2025-01-22
MX2024009056A (es) 2024-07-29
AU2019236246B2 (en) 2025-06-05
US20220308042A1 (en) 2022-09-29
PL3765846T3 (pl) 2025-03-31
ZA202005741B (en) 2023-04-26
AU2019236246A1 (en) 2020-11-12
ZA202208613B (en) 2025-01-29
EP4512887A3 (de) 2025-04-30
RU2020133815A (ru) 2022-04-19
WO2019178508A1 (en) 2019-09-19
BR112020018823A2 (pt) 2021-02-09
TWI835779B (zh) 2024-03-21
EP4512887A2 (de) 2025-02-26
ES3009097T3 (en) 2025-03-26
CN112136045A (zh) 2020-12-25
TW201947033A (zh) 2019-12-16
EP3765846B1 (de) 2024-10-30
CN119709931A (zh) 2025-03-28
IL277352B1 (en) 2025-04-01
HUE069711T2 (hu) 2025-04-28
IL277352A (en) 2020-11-30
KR20200132932A (ko) 2020-11-25
US20190285617A1 (en) 2019-09-19
JP2021515584A (ja) 2021-06-24
EP3765846A1 (de) 2021-01-20
MX2020009605A (es) 2021-01-08
IL277352B2 (en) 2025-08-01

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