EP3744401B1 - Pflanzenextraktionsverfahren enthaltend einen fermentationsschritt mit saccharomyces cerevisiae - Google Patents

Pflanzenextraktionsverfahren enthaltend einen fermentationsschritt mit saccharomyces cerevisiae Download PDF

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EP3744401B1
EP3744401B1 EP19305673.6A EP19305673A EP3744401B1 EP 3744401 B1 EP3744401 B1 EP 3744401B1 EP 19305673 A EP19305673 A EP 19305673A EP 3744401 B1 EP3744401 B1 EP 3744401B1
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Prior art keywords
extract
yeast
saccharomyces cerevisiae
bayanus
fermentation
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English (en)
French (fr)
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EP3744401A1 (de
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Alix Toribio
David Legangneux
Vincent Cocandeau
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Chanel Parfums Beaute SAS
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Chanel Parfums Beaute SAS
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Priority to EP19305673.6A priority Critical patent/EP3744401B1/de
Priority to JP2020088876A priority patent/JP2020193197A/ja
Priority to US16/883,058 priority patent/US11304891B2/en
Priority to CN202010456812.2A priority patent/CN112006928A/zh
Priority to KR1020200063898A priority patent/KR102611827B1/ko
Publication of EP3744401A1 publication Critical patent/EP3744401A1/de
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Publication of EP3744401B1 publication Critical patent/EP3744401B1/de
Priority to JP2023144985A priority patent/JP2023175749A/ja
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8967—Lilium, e.g. tiger lily or Easter lily
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34—Alcohols
    • A61K8/347—Phenols
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9794—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/10—General cosmetic use
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae

Definitions

  • the subject of the present invention is a process for the preparation of a plant extract enriched in polyphenols and/or substantially devoid of simple sugars.
  • the skin is mainly made up of three layers, namely, starting from the most superficial, the epidermis, the dermis and the hypodermis.
  • the epidermis is in particular made up of keratinocytes (mainly), melanocytes (intervening in the pigmentation of the skin) and Langerhans cells. Its function is to protect the body from the external environment and to ensure its integrity, and in particular to slow down the penetration of micro-organisms or chemical substances, and to prevent the evaporation of the water contained in the skin.
  • the keratinocytes undergo a continuous oriented maturation process during which the keratinocytes located in the basal layer of the epidermis form, at the terminal stage of their differentiation, corneocytes which are completely keratinized dead cells in the form of envelopes.
  • corneas made up of proteins and lipids such as ceramides.
  • intercorneocyte epidermal lipids are further formed and then organized as bilayers (sheets) in the stratum corneum. They participate, with the aforementioned horny envelopes, to the barrier function of the epidermis.
  • the barrier function of the epidermis can however be disturbed in certain climatic conditions (under the effect of cold and/or wind, for example), or even under the effect of stress or fatigue, in particular, thus favoring the penetration of allergens, irritants or micro-organisms which thus cause skin dryness likely to generate feelings of discomfort such as tightness or redness, and also to alter the radiance of the complexion and suppleness skin.
  • compositions containing hygroscopic agents such as sugars or polyols
  • these compositions also include active agents acting on one or more biological targets involved, for example, in the processes of skin aging.
  • Plant extracts have the advantage of containing many polyols and particularly polyphenols, whose antioxidant effects are now widely recognized.
  • plant extracts also contain many simple sugars (representing 25 to 75% by weight of conventional extracts) such as glucose, fructose and galactose, which have the effect of impairing the biological activity of plant actives.
  • plant extracts are generally treated using purification techniques such as the use of resins, activated carbon, cellulose or by distillation. These treatments aim to eliminate the polar matrix containing the simple sugars.
  • the applicant has succeeded in developing a process for the preparation of a plant extract enriched in polyphenols and substantially free of simple sugars.
  • the applicant has in fact demonstrated that it was possible to eliminate simple sugars and to increase the polyphenol content of a plant extract by carrying out a stage of fermentation of said extract with a yeast of the Saccharomyces cerevisiae var type. bayanus.
  • the present invention therefore relates to a process for the preparation of a plant extract enriched in polyphenols and/or substantially devoid of simple sugars, comprising a stage of fermentation of a plant extract by a yeast belonging to the variety Saccharomyces cerevisiae var. bayanus.
  • a plant extract capable of being obtained according to the method described above has the particularity of being substantially devoid of simple sugars but also of containing high levels of polyphenols such as flavonoids.
  • the present invention relates to a process for the preparation of a plant extract enriched in polyphenols and/or substantially devoid of simple sugars, said process comprising a stage of fermentation of a plant extract by a yeast belonging to the variety Saccharomyces cerevisiae var. bayanus.
  • Polyphenols are molecules comprising at least two phenolic groups, that is to say comprising at least two benzene rings carrying hydroxyl functions.
  • polyphenols mention may in particular be made of hydroxybenzoic acids, hydroxycinnamic and coumarin acids, naphthoquinones, stilbenoids or else flavonoids.
  • Flavonoids are the most represented polyphenols in the plant kingdom, they include in particular flavones, flavonols and dihydroflavonols, flavanones, aurones, chalcones and dihydrochalcones, flavanols, flavanediols, anthocyanidins, and their glycosides.
  • Polyphenols are recognized for their antioxidant effects, which make them particularly interesting anti-aging active ingredients.
  • the presence of many hydroxyl groups in polyphenols makes it possible to capture the water present in the skin and thus slow down its evaporation. Polyphenols therefore also have very interesting moisturizing effects.
  • an extract “enriched in polyphenols” is meant here an extract comprising from 5.00 to 100% of polyphenols by weight, particularly from 5.00 to 85.00% of polyphenols by weight, more particularly from 55.00 to 85.00% of polyphenols by weight.
  • the “simple sugars” are glucose, fructose, galactose and mannose, but also dimers of glucose, fructose, galactose and mannose.
  • an extract “substantially free of simple sugars” is a extract comprising less than 20.00% by weight, particularly less than 10.00% by weight, preferably less than 5.00% by weight, even more preferably less than 1.00% by weight of simple sugars.
  • the extract obtained is completely devoid of simple sugars, that is to say it comprises 0% by weight of simple sugars.
  • the present process comprises a stage of fermentation of a plant extract by a yeast belonging to the variety Saccharomyces cerevisiae var. bayanus. This fermentation step makes it possible to reduce or even eliminate the simple sugars contained in a plant extract, but also to enrich the content of the plant extract in polyphenols.
  • Saccharomyces cerevisiae var. bayanus makes it possible to obtain optimal fermentation kinetics for plant extracts, and above all much better than that obtained by using other varieties of yeasts.
  • Saccharomyces cerevisiae var. bayanus particularly make it possible to reduce the fermentation time of plant extracts, that is to say the time necessary for the consumption of the sugars in the extract, to less than 24 hours. These yeasts therefore make it possible to quickly and efficiently consume the simple sugars contained in the plant extracts.
  • yeast Saccharomyces cerevisiae var. bayanus are known and particularly used in the field of oenology. A person skilled in the art knows how to obtain such yeasts to implement the present invention.
  • the yeast Saccharomyces cerevisiae var. bayanus is chosen from yeast strains marketed under the references IOC Fizz+, IOC Meaning, IOC 18-2007 or a mixture thereof. All these strains are referenced by the French Institute of Vine and Wine and easily accessible for those skilled in the art.
  • the IOC Fizz+ yeast strain is a mixture of two yeasts deposited at the Pasteur Institute under the references LYCC 6022: LA CLAIRE CGC62 and LYCC 6039: LA CLAIRE SP665.
  • the IOC Tooc strain corresponds to the strain deposited at the Institut Pasteur under the reference LYCC 7000, and the strain 18-2007 to that deposited at the Institut Pasteur under the reference CNCM I-5320.
  • the yeast strains according to the present invention can be in dry form, in liquid form or in the form of yeast cream.
  • the strain of Saccharomyces cerevisiae var. bayanus is in dry form.
  • the fermentation step is done using the strain of Saccharomyces cerevisiae var. bayanus at between 1 and 5% by mass compared to the solution to be fermented, particularly at between 2 and 3% by mass relative to the solution to be fermented, and more particularly at 2% by mass relative to the solution to be fermented.
  • the duration of the fermentation step must be sufficient to make it possible to achieve a level of simple sugars of less than 20% by weight, particularly 10% by weight, preferably less than 5% by weight, even more preferably less than 1 % by weight in the plant extract.
  • the fermentation step is carried out over a period of 1 to 72 hours, particularly 12 to 60 hours, preferably 18 to 48 hours.
  • the fermentation step can be carried out using a sourdough.
  • This leaven is prepared from the fermentation of a concentrated plant extract (said plant extract being the same as that used in the process of the invention) with the strain of Saccharomyces cerevisiae var. bayanus.
  • the plant extract used for the sourdough is typically concentrated until a dry matter content of around 5 to 30% is obtained.
  • the fermentation step of the process according to the invention is carried out by adding this leaven to the plant extract.
  • the use of a leaven makes it possible to initiate the fermentation and thus to improve the kinetics of the fermentation step of the process according to the invention.
  • sourdough is prepared by fermenting for about 2 hours at room temperature (or alternatively at 37°C) a vegetable extract concentrated at between 5 and 50%, particularly at between 15 and 30% dry matter in water with 10 % by weight of dry yeast Saccharomyces cerevisiae var. bayanus. 1 to 5% of this leaven by weight, preferably 2% leaven by weight, are added to the plant extract for the fermentation step according to the process of the invention.
  • the plant extract used according to the method of the invention can be obtained according to any extraction method known to those skilled in the art.
  • the “plant” used in step a) can be any living being belonging to the plant kingdom.
  • the plant is a woody land plant or herbaceous plant.
  • the plant is a phanerogam or “flowering plant”. It can, for example, be chosen from lily ( Lilium candidum ), forget-me-not ( Myosotis sylvatica ), elderberry ( Sambucus sp. ), camelia ( Camellia sp. ), primrose ( Primula sp.
  • tuberose Polianthes tuberosa
  • jasmine Jasminum grandiflorum
  • plantain Plantaino lanceolata
  • quince Cydonia oblonga
  • apricot Prunus armeniaca
  • frangipani Plumeria sp.
  • the part of the plant extracted in step a) is the flower.
  • the plant extract according to the present invention is therefore a flower extract.
  • the plant used in step a) can be in "fresh" form, that is to say be used within 48 hours, particularly the 24 hours, even more particularly the 12 hours following the harvest.
  • the plant used in step a) can also be in dry form.
  • the fresh plant is dehydrated in mild conditions, either at room temperature away from light or in a ventilated dryer at a temperature below 35°C.
  • the plant is preferably dried until a dry matter content greater than 80% and preferably greater than 85% is obtained.
  • Step a) can also comprise the grinding of the flowers in order to obtain particles with a size of less than 5 cm, preferably less than 2 cm.
  • the extraction step is carried out with at least one extraction solvent comprising an alcoholic solvent and/or water.
  • the extraction solvent depends on the chosen plant. Plant/solvent ratio typically 1:10 (weight/weight).
  • the extraction solvent is water and/or an alcoholic solvent.
  • the alcoholic solvent is an alcohol such as ethanol, particularly 96° ethanol.
  • the extraction solvent can contain from 0 to 100% by volume of water and from 0 to 100% by volume of alcoholic solvent.
  • the extraction solvent comprises 100% water, in another embodiment, it comprises 100% ethanol.
  • the extraction solvent can also comprise 50% by volume of water and 50% by volume of ethanol.
  • the water can be deionized water.
  • the extraction step lasts at least 1 hour, preferably at least 2 hours, particularly at least 3 hours, and can be repeated once or twice.
  • the extraction process may comprise a step in which the mixture resulting from step b) is decolorized.
  • This step aims to eliminate the pigments present in the extract such as chlorophylls and xanthophylls.
  • the bleaching can, for example, be carried out by bringing the mixture into contact with activated carbon. Once the bleaching has been carried out, the mixture is filtered in order to eliminate the carbon residues.
  • step d) comprises concentrating the extract until a dry matter content in water of around 5 to 30%, preferably 15 to 30%, is obtained.
  • the plant extract from step d), that is to say the concentrated plant extract, can be used to prepare the sourdough mentioned above.
  • the process described above may also comprise a step d') between steps d) and e), in which the mixture containing the concentrated extract is finely filtered (typically at 2 ⁇ m) in order to remove fine particles and residual bacteria.
  • the method may also comprise a step f), in which the fermented extract is filtered and then diluted in order to obtain an extract containing 1 to 10% of dry matter by weight.
  • This extract may be in the form of a clear and stable aqueous solution.
  • the plant extract is used for cosmetic purposes, to moisturize human skin or protect it from drying out. Thanks to the antioxidant effects polyphenols, the extract can also be used to combat the signs of aging such as fine lines and wrinkles, loss of firmness and elasticity due to tissue loss in the epidermis and/or dermis; loss of radiance due to the reduction of microcirculation and a slowdown in cell renewal in the epidermis, the appearance of pigment spots associated with a dysfunction in melanin synthesis, or skin dryness resulting from a decrease in the barrier function of the stratum corneum and a slowdown in epidermal renewal.
  • signs of aging such as fine lines and wrinkles, loss of firmness and elasticity due to tissue loss in the epidermis and/or dermis; loss of radiance due to the reduction of microcirculation and a slowdown in cell renewal in the epidermis, the appearance of pigment spots associated with a dysfunction in melanin synthesis, or skin dryness resulting from a decrease in the barrier function of the stratum corne
  • Antioxidants are indeed known for their ability to trap free radicals which are one of the major factors in the acceleration of skin aging.
  • a cosmetic composition comprising a plant extract as previously described is disclosed here, but is not covered by the text of the claims.
  • said extract is present in the cosmetic or dermatological composition at a rate of 0.001 to 90% by total weight of the composition, in particular at a rate of 0.01 to 10%, preferably from 0.1 to 10% by weight composition total.
  • Said cosmetic or dermatological composition may in particular be suitable for topical application.
  • said cosmetic or dermatological composition may be in the form of a powder, an emulsion, a microemulsion, a nanoemulsion, suspension, solution, lotion, cream, aqueous or hydroalcoholic gel, foam, serum, solution or dispersion for aerosol , or a dispersion of lipid vesicles.
  • an emulsion it may be a water-in-oil or oil-in-water emulsion.
  • the cosmetic or dermatological composition may also comprise a solvent chosen according to the various ingredients and the form of administration.
  • water preferably demineralised water
  • an alcohol such as ethanol
  • a diethylene glycol ether such as ethoxydiglycol or diethylene glycol monomethyl ether.
  • Said cosmetic composition may also comprise, in addition to the extract, at least one additive customary in the field, such as for example at least one compound chosen from an emollient or humectant agent, a gelling and/or thickening agent, a surfactant , an oil, an active agent, a colorant, a preservative, an antioxidant agent, an active agent, an organic or inorganic powder, a sunscreen and a perfume.
  • at least one additive customary in the field such as for example at least one compound chosen from an emollient or humectant agent, a gelling and/or thickening agent, a surfactant , an oil, an active agent, a colorant, a preservative, an antioxidant agent, an active agent, an organic or inorganic powder, a sunscreen and a perfume.
  • additives usually used in cosmetics may also be present in the composition, in particular preservatives, antioxidants or perfumes well known in the technical field.
  • Figure 1 Linear regression of the total sugar content consumed during fermentation for each of the strains studied (reference strain Saccharomyces cerevisiae not belonging to the variety bayanus [ref], and the strains Saccharomyces cerevisiae var. bayanus 18-2007, Fizz+ and Too )
  • Table 1 Composition of a Lys extract according to the invention Lily flowers (Lilium candidum) Molecular family Molecules Conventional dry extract Conventional extract diluted to 1% Dry fermented extract Fermented extract diluted to 1% Saccharides Fructose, Glucose, Sucrose 58.00% 0.58% 0.00% 0.00% Nitrogen and others Lilidine glucoside, Glucopyranosyl glycerol, Hopantenic acid 15.30% 0.15% 48.60% 0.48% Flavonoids and other polyphenols Kaempferol di and triglucosides, Quercetin diglucosides, Helionosides A, Regaloside A 3.50% 0.03% 9.00% 0.09% Lipids Fatty acids C16:0, C18:0, C18:1, C18:2, C18:3 3.00% 0.03% 0.00% 0.00% Others Others 19.70% 0.20% 42.40% 0.42%
  • the process according to the present invention makes it possible to completely eliminate simple sugars from an extract of lily flowers.
  • the process according to the invention also makes it possible to greatly increase the polyphenol content of the extract.
  • EXAMPLE 2 The kinetics of fermentation of dry yeast from Saccharomyces cerevisiae var. bayanus Fizz+, Meaning and 18-2007 allow optimal fermentation of flower extracts.
  • the reference strain which does not belong to the variety Saccharomyces cerevisiae var. bayanus, consumes total sugars two to three times slower than the strains Fizz+, Meaning and 18-2007 which belong to the variety Saccharomyces cerevisiae var. bayanus.
  • Saccharomyces cerevisiae var. bayanus make it possible to obtain optimal kinetics of fermentation of plant extracts.
  • composition can be prepared in a conventional manner for those skilled in the art.
  • quantities indicated below are expressed in percentages by weight.
  • Ingredients in capital letters are identified according to the INCI denomination.

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Claims (8)

  1. Verfahren zur Herstellung eines Blütenextrakts, der mit Polyphenolen angereichert und/oder im Wesentlichen frei von einfachen Zuckern ist, umfassend einen Schritt der Fermentation eines Blütenextrakts mit einer Hefe, die zur Varietät Saccharomyces cerevisiae var. bayanus gehört.
  2. Verfahren nach Anspruch 1, das vor dem Fermentationsschritt die Herstellung eines Blütenextrakts mittels der folgenden Schritte umfasst:
    a) Extraktion von wenigstens einer Blüte mit wenigstens einem alkoholischen Lösungsmittel und/oder Wasser;
    b) Filtrieren der in a) erhaltenen Mischung, um pflanzliche Rückstände zu entfernen;
    c) gegebenenfalls Entfärben der in Schritt b) erhaltenen Mischung; und
    d) Entfernen des Lösungsmittels und Konzentrieren des erhaltenen Extrakts.
  3. Verfahren nach einem der Ansprüche 1 oder 2, wobei die Hefevarietät Saccharomyces cerevisiae var. bayanus ausgewählt ist aus der Gruppe, die aus Hefen der Stämme IOC Fizz+, IOC Divine, IOC 18-2007 und Mischungen davon gebildet ist.
  4. Verfahren nach Anspruch 3, wobei die Hefevarietät Saccharomyces cerevisiae var. bayanus eine Hefe des Stamms IOC Fizz+ ist.
  5. Verfahren nach Anspruch 3, wobei die Hefevarietät Saccharomyces cerevisiae var. bayanus eine Hefe des Stamms IOC Divine ist.
  6. Verfahren nach Anspruch 3, wobei die Hefevarietät Saccharomyces cerevisiae var. bayanus eine Hefe des Stamms IOC 18-2007 ist.
  7. Verfahren nach einem der Ansprüche 1 bis 6, wobei die Blüte eine Blüte aus Lilium candidum, Myosotis sylvatica, Sambucus sp., Camellia sp., Primula sp., Polianthes tuberosa, Jasminum grandiflorum, Plantago lanceolata, Cydonia oblonga, Prunus armeniaca oder Plumeria sp. ist.
  8. Verfahren nach einem der Ansprüche 1 bis 7, wobei der Fermentationsschritt mit Hilfe eines Sauerteigs durchgeführt wird, der aus der Fermentation eines konzentrierten Pflanzenextrakts mit dem Stamm Saccharomyces cerevisiae var. bayanus hergestellt wurde.
EP19305673.6A 2019-05-28 2019-05-28 Pflanzenextraktionsverfahren enthaltend einen fermentationsschritt mit saccharomyces cerevisiae Active EP3744401B1 (de)

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EP19305673.6A EP3744401B1 (de) 2019-05-28 2019-05-28 Pflanzenextraktionsverfahren enthaltend einen fermentationsschritt mit saccharomyces cerevisiae
JP2020088876A JP2020193197A (ja) 2019-05-28 2020-05-21 植物体抽出方法
US16/883,058 US11304891B2 (en) 2019-05-28 2020-05-26 Process for extracting plants
CN202010456812.2A CN112006928A (zh) 2019-05-28 2020-05-26 提取植物的方法
KR1020200063898A KR102611827B1 (ko) 2019-05-28 2020-05-27 식물 추출 방법
JP2023144985A JP2023175749A (ja) 2019-05-28 2023-09-07 植物体抽出方法

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CN114272176A (zh) * 2021-12-29 2022-04-05 重庆佰东生物科技有限公司 植物提取物及其提取工艺和应用

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KR102611827B1 (ko) 2023-12-07
US11304891B2 (en) 2022-04-19
JP2020193197A (ja) 2020-12-03
JP2023175749A (ja) 2023-12-12
US20200375879A1 (en) 2020-12-03
EP3744401A1 (de) 2020-12-02
CN112006928A (zh) 2020-12-01

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