EP3663001B1 - Methode de pipetage pourvu de point d'étranglement dans le canal de pipetage - Google Patents

Methode de pipetage pourvu de point d'étranglement dans le canal de pipetage Download PDF

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Publication number
EP3663001B1
EP3663001B1 EP20152812.2A EP20152812A EP3663001B1 EP 3663001 B1 EP3663001 B1 EP 3663001B1 EP 20152812 A EP20152812 A EP 20152812A EP 3663001 B1 EP3663001 B1 EP 3663001B1
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EP
European Patent Office
Prior art keywords
pipetting
dosing liquid
dosing
working fluid
pressure
Prior art date
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EP20152812.2A
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German (de)
English (en)
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EP3663001A1 (fr
Inventor
Vinzenz Kirste
Helmut Knapp
Urs Lendenmann
Noa Schmid
Tobias Seiler
Fabian Winter
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Tecan Trading AG
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Tecan Trading AG
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Publication of EP3663001A1 publication Critical patent/EP3663001A1/fr
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/148—Specific details about calibrations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof

Definitions

  • the present invention relates to a pipetting device at least for dispensing dosing liquid by increasing the pressure of a working fluid, comprising a dosing liquid receiving space which is at least partially filled with working fluid and has a pipetting opening as a first flow cross-section constriction, through which dosing liquid flows out of the dosing liquid chamber as a function of the pressure of the working fluid.
  • Receiving space can also be dispensed, and a pressure changing device which is designed to change the pressure of the working fluid in the dosing liquid receiving space.
  • the pipetting opening generally represents a narrowest flow cross-section when the dosing liquid is expelled from the dosing-liquid receiving space, the pipetting opening forms a first flow cross-section constriction of the pipetting device discussed here.
  • Pipetting devices of the type mentioned at the outset are used, for example, as washing heads, in which the dosing liquid receiving space is filled or at least partially filled by a dosing liquid feed and then expelled from the latter by the described dispensing by means of excess pressure of the working fluid relative to the ambient pressure of the liquid receiving space.
  • the dosing liquid is a washing liquid which is dispensed through the pipetting opening in order to clean an object provided underneath, for example a container, with the washing liquid.
  • the washing liquid is a washing liquid which is dispensed through the pipetting opening in order to clean an object provided underneath, for example a container, with the washing liquid.
  • washing heads in addition to or as an alternative to the aforementioned inflow, by aspiration known per se, i.e. by means of a negative pressure of the working fluid in the dosing liquid receiving space, through the pipetting opening and into the dosing liquid receiving space record, tape.
  • aspiration known per se i.e. by means of a negative pressure of the working fluid in the dosing liquid receiving space, through the pipetting opening and into the dosing liquid receiving space record, tape.
  • this object is achieved by a pipetting method in which the pipetting device has a throttle point as a further flow cross-section constriction in a pipetting channel that is operationally filled with working fluid, fluid-mechanically between the dosing liquid receiving space and the pressure-changing device, which is dimensioned in such a way that a ratio of a flow resistance ( R 1 ) of the pipetting opening for dispensed dosing liquid to a flow resistance (R 2 ) of the throttle point for working fluid, which flows through the throttle point when dispensing the dosing fluid, is less than 0.5, preferably less than 0.3, particularly preferably less than 0.225, the flow resistances of the respective flow cross-section constriction being calculated taking into account the product of the viscosity of the medium of working fluid and dosing liquid associated with the respective flow cross-section constriction t and the characteristic length of the associated flow area restriction divided by the fourth power of the characteristic dimension of the flow area of the associated flow area restriction.
  • the ratio of the flow resistances mentioned which is decisive for the functioning of the solution presented here, means that the throttle point through which the working fluid, usually a gas, flows has a significantly smaller cross-sectional opening than the pipetting opening.
  • a liquid is also used as the working fluid.
  • the viscosity to be used is the respective dynamic viscosity, which is generally denoted in the literature by the symbol “ ⁇ ”.
  • the designated characteristic length of the associated cross-sectional constriction can be the length of the cylindrical duct or, in the case of ducts tapering conically towards the flow cross-sectional constriction, it can be the length of the duct section in which the flow cross-sectional area of the duct, starting from the smallest flow cross-sectional area in the throttle point or in the Pipette opening doubled. If the flow cross-sectional area does not double over the maximum ascertainable length of the channel, the entire length of the channel can be used as the characteristic length.
  • the characteristic dimension of the flow cross-section can be the diameter for circular flow cross-sections, an edge length for square flow cross-sections, an arithmetic mean of long and short edge length for rectangular flow cross-sections, an arithmetic mean of long and short axis for elliptical flow cross-sections, etc. If the flow cross section changes over the length of the flow cross section constriction, the smallest flow cross section occurring in the flow cross section constriction should be used.
  • the present invention preferably relates to a washing head pipetting device already mentioned at the outset, which is designed to deliver washing liquid as dosing liquid in precise doses.
  • washing-head pipetting devices are generally used to clean objects held in sample containers, such as so-called "wells", by dispensing a precisely metered quantity of washing liquid.
  • the precise dosing of the washing liquid is of great importance in order to bring about a predetermined cleaning state.
  • the volume flow of washing liquid is set in such a way that the washing performance is as great as possible, but the duration of the washing process is as short as possible. If washing liquid is pipetted incorrectly, there may be a risk of so-called “overwashing”, which can lead to elements being undesirably dissolved on the object to be washed and/or in the sample container.
  • washing head pipetting device aspirates the washing liquid as dosing liquid via the pipetting opening into the dosing liquid receiving space, for reasons of simpler handling the dosing liquid receiving space of a preferred washing head pipetting device is supplied with washing liquid as dosing liquid through a dosing liquid inlet.
  • the washing head pipetting device discussed here can therefore have a dosing liquid inlet through which the dosing liquid receiving space can be at least partially filled with dosing liquid, ie with washing liquid in the present application.
  • the dosing liquid feed opens into the dosing liquid receiving space.
  • the dosing liquid inlet is generally provided as a channel formed separately from the dosing liquid receiving space.
  • the washing head pipetting device can have a dosing liquid pump in a further advantageous embodiment of the present invention, with which dosing liquid, in particular washing liquid as dosing liquid, can be conveyed along the dosing liquid feed into the dosing liquid receiving space.
  • a valve can be provided on the dosing liquid feed, in particular in an area close to the mouth, which can be opened and closed by a control device.
  • the dosing liquid pump can also be operated with this or another control device.
  • the dosing liquid pump mentioned above does not have to be provided, since the dosing liquid, in particular as washing liquid, is pumped from a geodetic over the mouth of the dosing liquid inlet into the dosing liquid receiving space gravity can be promoted. Then, however, the valve mentioned above is absolutely necessary.
  • a washing head pipetting device In order to increase the washing efficiency of a washing head pipetting device, it can have a plurality of pipetting channels which are provided essentially parallel to one another, so that a plurality of objects corresponding to the plurality of pipetting channels can be subjected to cleaning by the washing head pipetting device at the same time.
  • the present invention is particularly advantageous in the case of a multi-channel pipetting device, since the invention can ensure that each pipetting channel can deliver essentially the same amount of dosing liquid with a high level of accuracy, although the individual pipetting channels, be it due to manufacturing tolerances coupled to pipetting tips it can have different geometric shapes due to different levels of deposits on the pipetting openings, be it a combination of these or other causes, so that without the present invention being used, the individual pipetting channels of a multi-channel pipetting head would deliver different pipetting results with the same operating parameters of the pipetting device.
  • the principle of throttling the working fluid flow between the dosing liquid receiving space and the pressure-changing device can be used successfully not only in the dispensing of dosing liquids, but also in their aspiration.
  • the dosing behavior can be insensitive to deposits and other flow cross-section changes in the pipette opening.
  • the present invention relates in particular to those pipetting devices which, in addition to the above-mentioned dispensing, are also used to aspirate metering liquid, in this case by reducing the Pressure of the working fluid in the dosing liquid receiving space are formed.
  • dosing liquid when dosing liquid is aspirated, it can be aspirated through the pipetting opening into the dosing liquid receiving space as a function of the pressure of the working fluid.
  • the throttle point recommended here in the pipetting channel is fluid-mechanically between the pressure-changing device and the dosing liquid receiving space, within certain limits, also suitable for equalizing the dosing behavior across dosing liquids with different viscosities.
  • the dosing behavior of these pipetting devices is independent of the viscosity of the dosing liquid within certain limits.
  • Working fluids can be used successfully here whose dynamic viscosity does not exceed the value of 0.00003 Nsm -2 , preferably 0.00002 Nsm -2 , particularly preferably 0.0000175 Nsm -2 .
  • the dynamic viscosity is meant.
  • the throttle point can also be considered to equip the throttle point with a flow cross section that can be changed as desired, for example by changing the gap width of an annular gap or with a mechanism similar to that used to adjust the aperture on mechanical cameras.
  • the flow cross section of the throttle point can thus be adapted to the working fluid used in each case and/or the dosing liquid to be metered in each case.
  • the pipetting channel can be adjusted between a blocked position, in which a working fluid flow in the pipetting channel is prevented, and an open position, in which a working fluid flow in the pipetting channel is permitted valve.
  • the valve can initially be closed be maintained until the working fluid has been brought to a desired pressure in an area at least close to the pressure-changing device.
  • the throttle point can be variable down to a cross section of zero, so that the valve described here can be implemented using an advantageously small number of components through the throttle point described above with a variable flow cross section.
  • a quantity of liquid can be metered with high precision in a manner known per se by intermittently opening and closing the valve.
  • At least one reservoir of working fluid that is under a system pressure can be provided as the pressure-changing device. More precisely, in order to carry out both aspiration and dispensing processes on one and the same pipetting channel, a dispensing reservoir under a first system pressure and an aspiration reservoir under a second system pressure can be provided, which can optionally be connected to the pipetting channel in a pressure-transmitting manner and are separable therefrom, wherein the first system pressure is greater than an ambient pressure of the pipetting device and the second system pressure is smaller than the ambient pressure.
  • the ambient pressure of the dosing liquid receiving space does not exceed an overpressure of 1.5 bar, preferably 1.2 bar, particularly preferably 1.0 bar.
  • an overpressure of 1.5 bar preferably 1.2 bar, particularly preferably 1.0 bar.
  • Higher pressure differences between the system pressure and the ambient pressure can lead to highly turbulent flows of the working fluid through the throttling point, which under certain circumstances can impair the effect of the present invention.
  • the pressure-changing device has a discontinuously or continuously operating pump, optionally in interaction with a valve arrangement that is arranged in the conveying path of the pump and can be selectively opened and closed.
  • a valve arrangement that is arranged in the conveying path of the pump and can be selectively opened and closed.
  • the pressure changing device has a piston-cylinder device with a cylinder extending along a cylinder axis and with a piston accommodated therein so that it can move along the cylinder axis, with the cylinder and piston delimiting at least one working volume , which can be changed relative to the cylinder by a relative movement of the piston and which is or can be brought into fluid communication with the pipetting channel.
  • the piston-cylinder arrangement represents the most common design of the pressure-changing device in pipetting devices. It also offers the possibility of very precise pressure control through the use of small piston areas and relatively long piston strokes.
  • the pipetting device can also be a manually actuated pipetting device as intended, in which case the piston in particular can then be moved relative to the cylinder by manual actuation.
  • This actuation can be immediate, ie by pulling it out or pressing in the piston by hand, or can take place indirectly, for example by tensioning a spring which drives a relative movement between the piston and the cylinder after it has been triggered.
  • the manually operable pipetting device preferably has only exactly one pipetting channel for the most precise possible dosing.
  • Manually operable as intended is not intended to cover cases that are generally operated by a motor or otherwise automatically and can only continue to be operated by manual emergency actuation in the event of a power supply failure.
  • the dosing liquid receiving space and the pipette opening are formed on a pipette tip that is separate from the pipette channel that has the throttle point and can be optionally connected to and/or separated from this pipette tip are.
  • outlets provided rigidly with the pipetting device so-called “washing tubes”, are preferred for the aforementioned washing head pipetting device.
  • FIG 1 an embodiment of a pipetting device according to the invention shown in a roughly schematic manner is generally denoted by 10 .
  • the pipetting device 10 comprises a pipetting channel 12 to which a pipetting tip 14 is detachably coupled in a manner known per se.
  • the pipetting channel 12 has a cylinder section 16 in which a piston 18 can be moved along a longitudinal axis which coincides with the cylinder axis Z L of the pipetting device 10 can be adjusted relative to the cylinder section 16 via a piston rod 20 by a motor 22 .
  • the motor 22 is controlled by a control/regulating unit 24, for example as a function of the detection signal from a pressure sensor 26, which detects the pressure of a working fluid, such as air, in a working chamber 28 whose volume is variable as a result of the movement of the piston 18.
  • a control/regulating unit 24 for example as a function of the detection signal from a pressure sensor 26, which detects the pressure of a working fluid, such as air, in a working chamber 28 whose volume is variable as a result of the movement of the piston 18.
  • the pipette tip 14 which can be detached from the pipette channel 12 in a manner known per se by means of an ejector 30 that is movable along the longitudinal axis L of the pipette device 10 relative to the cylinder section 16, has a coupling region 32 designed for coupling to the pipette channel 12, a figure 1 example shown, has a conically extending wall area 34 and a pipetting opening 36, through which a dosing liquid, depending on the pressure of a working fluid with which a dosing liquid receiving space 38 surrounded by the wall area 34 and optionally also by the coupling area 32 is at least partially filled, into the dosing liquid -Receiving space can be aspirated and dispensed from this.
  • the assembly of cylinder 16 and piston 18 forms a pressure changing device 40 for changing the pressure of the working fluid in the dosing liquid receiving space 38.
  • the throttle point 42 can also have a valve 44 with which the throttle point 42 can be completely closed in order to interrupt a pressure propagation of the working fluid pressure from the working chamber 28 into the dosing liquid receiving chamber 38 .
  • the valve 44 can preferably also be actuated by the control/regulating device 24 .
  • ⁇ Pof is the dynamic viscosity of the medium flowing through the pipetting opening 36, i.e. the dosing liquid
  • I Pof is a characteristic length of an outlet end of the pipetting tip 14 leading to the pipetting opening 36
  • d Pof is a characteristic dimension of the flow cross section of the pipetting opening 36, in the present standard case a circular pipetting opening is the diameter of the pipetting opening 36 .
  • the characteristic length I is the length that the exit end of the pipette tip 14 has, starting from the pipette opening 36 to the point at which the flow cross section of the pipette tip 14 has twice the surface area of the pipette opening 36.
  • the characteristic length of an outlet area that tapers conically or in some other way towards the respective flow cross-section constriction with the narrowest flow cross-section can be assumed to be the length that exists between the respective flow cross-section constriction and a flow cross-section whose diameter is ⁇ 2 times the diameter of the flow cross-section constriction.
  • the ratio of the two flow resistances at the throttle point 42 and the pipette opening 36 is a ratio of 0.5, preferably does not exceed 0.3, particularly preferably 0.225
  • the dispensing behavior of the pipette tip 14, which can also be rigidly connected to the pipette channel 12 is largely independent of changes in the flow cross section, for example due to deposits of dried and/or crystallized dosing liquid.
  • the dosing behavior changes with increasing narrowing of the pipetting opening 36 from below a critical degree of narrowing, even despite the provision of the throttle point 42 in the pipetting channel 12 fluid-mechanically between the pressure varying device 40 and the dosing liquid receiving space 38.
  • the limit from which the influences of such deposits can of the pipetting opening 36 or in a region close to the pipetting opening 36 during dispensing, can be pushed further in the direction of a reduction in the cross-section of the pipetting opening 36 .
  • the aspiration and dispensing behavior of the pipetting device can even be independent of the viscosity within certain limits used dosing liquid are made, so that with one and the same pipetting device 10 and one and the same operating parameters different viscous dosing liquids can be dosed the same. This considerably simplifies the operation of pipetting devices.
  • the present invention can be used in particular for dosing tasks which a pipetting device 10 has to fulfill as a so-called “washing-head pipetting device” if this washing liquid is to be dispensed in precise doses as dosing liquid.
  • washing head pipetting devices can be used to clean objects 37 in sample containers 39 or sample containers 39 alone, which are usually located below the pipetting opening 36, in a defined manner by dispensing a measured amount of washing liquid as dosing liquid.
  • such a washing head pipetting device 10 can have a dosing liquid inlet 50 which, starting from a dosing liquid reservoir 52 , can open out at an orifice 54 into the dosing liquid receiving space 38 .
  • the dosing liquid receiving space can advantageously be filled with dosing liquid (then in the form of washing liquid) through the dosing liquid inlet 50 so that the dosing liquid does not have to be aspirated through the pipetting opening 36 in this case.
  • the dosing liquid in the dosing liquid reservoir 52 can be conveyed by a pump 56, which can also be controlled by the control/regulating device 24, via the dosing liquid inlet 50 into the dosing liquid receiving space 38.
  • a valve 58 can also be provided on the dosing liquid inlet 50 which can be opened and closed by the control/regulating device 24 .
  • a suitable program in the control/regulating device can initially build up a predetermined pressure in the dosing liquid inlet 50 by operating the pump 56 with the valve 58 closed, whereupon the valve 58 is opened for a predetermined time and then closed again.
  • the valve 58 is preferably arranged at the orifice 54 or, in relation to the total length of the dosing liquid feed, close to the orifice 54.
  • the distance of the valve 58 from the orifice 54 should preferably not exceed 5% of the total length of the dosing liquid feed 58 .
  • the pipetting device 50 in particular as a washing-head pipetting device 10, can have further pipetting channels in addition to the pipetting channel 12 shown, which are configured essentially identically to the pipetting channel 12 shown, so that the pipetting channel shown in 1 illustrated pipetting channel 12 is described as an example for all pipetting channels of a multi-channel pipetting device.
  • the dosing liquid feeds 50 to each pipetting channel 12 can then be connected to a common dosing liquid reservoir 52 via a common pump 56 .
  • all piston rods 20 of the individual pipetting channels 12 can be adjusted by a common motor 22 .
  • each pipetting channel has its own motor 22, its own pump 56 and/or its own supply of dosing liquid 52.
  • the fixing means 60 is intended to indicate that the pipette tip 34 can be coupled to the pipette channel 12 as a washing tube permanently and not detachably.
  • the washing tube can also be designed in one piece with a tube of the pipetting channel, for example with the cylinder section 16.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Claims (14)

  1. Procédé de pipetage comprenant les étapes consistant à :
    - fournir un espace de réception de liquide de dosage (38) présentant une ouverture de pipetage (36) comme premier rétrécissement de la section transversale d'écoulement, l'espace de réception de liquide de dosage fourni étant rempli au moins en partie avec un fluide de travail, et
    - aspirer le liquide de dosage dans l'espace de réception de liquide de dosage (38) à travers l'ouverture de pipetage (36) en abaissant la pression du fluide de travail à l'aide d'un dispositif de variation de pression (40) qui est conçu pour modifier la pression du fluide de travail dans l'espace de réception de liquide de dosage (38), un point d'étranglement (42) étant disposé dans un canal de pipetage (12) agencé dans un mécanisme de fluide entre l'espace de réception de liquide de dosage (38) et le dispositif de variation de pression (40),
    dans lequel le fluide de travail, lorsqu'il est aspiré, s'écoule à travers le point d'étranglement (42) comme autre rétrécissement de la section transversale d'écoulement,
    caractérisé en ce que le canal de pipetage (12) présente une soupape pouvant être réglée entre une position fermée, dans laquelle l'écoulement du fluide de travail dans le canal de pipetage est empêché, et une position ouverte, dans laquelle l'écoulement du fluide de travail dans le canal de pipetage est autorisé, en ce qu'il est déterminé une quantité du liquide de dosage sur un laps de temps au cours duquel la soupape est ouverte, et en ce que le rapport entre la résistance à l'écoulement (R1) de l'ouverture de pipetage (36) concernant le liquide de dosage et la résistance à l'écoulement (R2) du point d'étranglement (42) concernant le fluide de travail est inférieur à 0,5, en particulier inférieur à 0,3, en particulier inférieur à 0,225.
  2. Procédé de pipetage selon la revendication 1, consistant en outre à distribuer au moins une partie du liquide de dosage aspiré à travers l'ouverture de pipetage (36) depuis l'espace de réception de liquide de dosage (38) en augmentant la pression du fluide de travail à l'aide du dispositif de variation de pression (40), le fluide de travail s'écoulant à travers le point d'étranglement (42) comme autre rétrécissement de la section transversale d'écoulement aussi bien à l'aspiration qu'à la distribution.
  3. Procédé de pipetage selon l'une des revendications 1 ou 2, dans lequel la viscosité du liquide de dosage ne dépasse pas la valeur de 0,004 Nsm-2, de préférence la valeur de 0,0035 Nsm-2, de façon particulièrement préférée ne dépasse pas la valeur de 0,0031 Nsm-2.
  4. Procédé de pipetage selon l'une des revendications 1 à 3, dans lequel la viscosité du fluide de travail ne dépasse pas la valeur de 0,00003 Nsm-2, de préférence la valeur de 0,00002 Nsm-2, de façon particulièrement préférée ne dépasse pas la valeur de 0,0000175 Nsm-2.
  5. Procédé de pipetage selon l'une des revendications 1 à 4, dans lequel le fluide de travail est un gaz.
  6. Procédé de pipetage selon la revendication 5, dans lequel la quantité du liquide de dosage est dosée avec grande précision en ouvrant et en fermant la soupape de façon intermittente.
  7. Procédé de pipetage selon l'une des revendications 5 ou 6, dans lequel le dispositif de variation de pression (40) présente un fluide de travail se trouvant sous une pression de système.
  8. Procédé de pipetage selon la revendication 7, dans lequel il est prévu un réservoir de distribution sous une première pression de système et un réservoir d'aspiration sous une deuxième pression de système qui sont au choix reliés par transmission de pression au canal de pipetage (12) pour augmenter ou abaisser la pression du fluide de travail ou bien séparés de ce dernier, la première pression de système étant supérieure à la pression ambiante du dispositif de pipetage (10), et la deuxième pression de système étant inférieure à la pression ambiante.
  9. Procédé pour fabriquer une dose d'un liquide de dosage comprenant le procédé de pipetage selon l'une des revendications 2 à 8 et comprenant l'étape de distribution selon la revendication 2, dans lequel à l'étape de distribution la dose est distribuée.
  10. Procédé pour fabriquer une pluralité de doses d'un liquide de dosage, les doses comprenant sensiblement la même quantité de liquide de dosage, dans lequel chaque dose de la pluralité de doses est fabriquée selon le procédé de la revendication 9, et dans lequel la pluralité de doses comprend au moins une première dose distribuée à partir d'une première ouverture de pipetage et une deuxième dose distribuée à partir d'une deuxième ouverture de pipetage.
  11. Procédé selon la revendication 10, dans lequel la première et la deuxième ouverture de pipetage sont réalisées au niveau d'un premier et d'un deuxième embout de pipetage d'une tête de pipetage multicanaux, ou dans lequel la première et la deuxième ouverture de pipetage sont réalisées au niveau de deux dispositifs de pipetage différents, de construction sensiblement identique.
  12. Procédé selon l'une des revendications 10 ou 11, dans lequel les étapes du procédé de pipetage sont réalisées avec des paramètres de fonctionnement identiques pour chaque dose de la pluralité de doses.
  13. Procédé selon la revendication 9 pour fabriquer une première dose d'un premier liquide de dosage avec une première viscosité et une deuxième dose d'un deuxième liquide de dosage avec une deuxième viscosité, différente de la première viscosité, dans lequel la première dose est fabriquée au cours d'une première opération de dosage et la deuxième dose est fabriquée au cours d'une deuxième opération de dosage, dans lequel la première et la deuxième dose comprennent sensiblement la même quantité de liquide de dosage, et dans lequel dans la première et dans la deuxième opération de dosage, les étapes du procédé de pipetage selon l'une des revendications 1 à 8 sont réalisées avec des paramètres de fonctionnement identiques.
  14. Procédé selon la revendication 13, dans lequel un premier rapport entre la résistance à l'écoulement (R1) de l'ouverture de pipetage (36) concernant le premier liquide de dosage et la résistance à l'écoulement (R2) du point d'étranglement (42) concernant le fluide de travail, et un deuxième rapport entre la résistance à l'écoulement (R1) de l'ouverture de pipetage (36) concernant le deuxième liquide de dosage et la résistance à l'écoulement (R2) du point d'étranglement (42) concernant le fluide de travail est inférieur à 0,001, de préférence inférieur à 0,00075, et de façon davantage préférée inférieur à 0,0005.
EP20152812.2A 2010-07-26 2011-07-06 Methode de pipetage pourvu de point d'étranglement dans le canal de pipetage Active EP3663001B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102010038414A DE102010038414A1 (de) 2010-07-26 2010-07-26 Pipettiervorrichtung mit Drosselstelle im Pipettierkanal
EP11172869.7A EP2412439B1 (fr) 2010-07-26 2011-07-06 Dispositif de pipetage doté d'un poste d'étranglement dans le canal de pipetage

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EP11172869.7A Division EP2412439B1 (fr) 2010-07-26 2011-07-06 Dispositif de pipetage doté d'un poste d'étranglement dans le canal de pipetage

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Publication number Publication date
EP2412439B1 (fr) 2020-03-04
US20120017704A1 (en) 2012-01-26
US8656792B2 (en) 2014-02-25
EP2412439A1 (fr) 2012-02-01
JP2017070950A (ja) 2017-04-13
JP6463386B2 (ja) 2019-01-30
EP3663001A1 (fr) 2020-06-10
JP2012068231A (ja) 2012-04-05
DE102010038414A1 (de) 2012-01-26

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