EP2412439B1 - Dispositif de pipetage doté d'un poste d'étranglement dans le canal de pipetage - Google Patents

Dispositif de pipetage doté d'un poste d'étranglement dans le canal de pipetage Download PDF

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Publication number
EP2412439B1
EP2412439B1 EP11172869.7A EP11172869A EP2412439B1 EP 2412439 B1 EP2412439 B1 EP 2412439B1 EP 11172869 A EP11172869 A EP 11172869A EP 2412439 B1 EP2412439 B1 EP 2412439B1
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EP
European Patent Office
Prior art keywords
pipetting
pressure
receiving space
working fluid
dosing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP11172869.7A
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German (de)
English (en)
Other versions
EP2412439A1 (fr
Inventor
Vinzenz Kirste
Helmut Knapp
Urs Lendenmann
Noa Schmid
Tobias Seiler
Fabian Winter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tecan Trading AG
Original Assignee
Tecan Schweiz AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tecan Schweiz AG filed Critical Tecan Schweiz AG
Priority to EP20152812.2A priority Critical patent/EP3663001B1/fr
Priority to JP2011161908A priority patent/JP2012068231A/ja
Priority to US13/190,686 priority patent/US8656792B2/en
Publication of EP2412439A1 publication Critical patent/EP2412439A1/fr
Priority to JP2017001365A priority patent/JP6463386B2/ja
Application granted granted Critical
Publication of EP2412439B1 publication Critical patent/EP2412439B1/fr
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/148—Specific details about calibrations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof

Definitions

  • the present invention relates to a pipetting device at least for dispensing dosing liquid by increasing the pressure of a working fluid, comprising a dosing liquid receiving space which is at least partially filled with working fluid and has a pipetting opening as a first flow cross-section constriction, through which dosing liquid depends on the pressure of the working fluid from the dosing liquid.
  • Receiving space is dispensable, and a pressure changing device which is designed to change the pressure of the working fluid in the metering liquid receiving space.
  • Such pipetting devices are well known in the prior art.
  • a dosing liquid present in a dosing liquid receiving space is ejected through a pipetting opening of the dosing liquid receiving space by increasing a pressure of a working fluid, which is also located in the dosing liquid receiving space.
  • the pipetting opening generally represents a narrowest flow cross-section when expelling the dosing liquid from the dosing liquid receiving space, the pipetting opening forms a first flow cross-sectional constriction of the pipetting device discussed here.
  • Pipetting devices of the type mentioned at the outset are used, for example, as washing heads in which the metering liquid receiving space is filled or at least partially filled by a metering liquid inlet and then expelled from the latter by the described dispensing by means of excess pressure of the working fluid based on the ambient pressure of the liquid receiving space.
  • the metering liquid is a washing liquid which is dispensed through the pipetting opening in order to use the washing liquid to clean an object, such as a container, provided underneath.
  • the pipetting opening in order to use the washing liquid to clean an object, such as a container, provided underneath.
  • washing heads in addition or as an alternative to the previously mentioned inlet, the metering liquid, for example washing liquid, by aspiration known per se, that is to say by means of a negative pressure of the Absorb working fluids in the dosing liquid receiving space through the pipetting opening into the dosing liquid receiving space.
  • a manual pipetting device which allows the automated dispensing of predetermined volumes of a liquid.
  • the hand-held pipetting device is also designed for manual dispensing and aspirating.
  • a throttle is provided in an air line between an overpressure outlet of a pressure source provided for this purpose and a pipette holder for the pipette.
  • this object is achieved by a pipetting device according to claim 1.
  • the ratio of the flow resistances mentioned which is decisive for the functioning of the solution presented here, means that the throttle point through which working fluid, usually a gas, flows has a significantly smaller cross-sectional opening than the pipetting opening.
  • a liquid is also used as the working fluid.
  • the respective dynamic viscosity can be used as the viscosity, which is generally designated in the literature with the formula symbol " ⁇ ".
  • the characteristic length of the assigned cross-sectional constriction can be the length of the cylindrical channel in the case of cylindrical flow cross-sectional constrictions, or can be the length of the channel section in which the flow cross-sectional area of the channel starts from the smallest flow cross-sectional area in the throttle point or in the case of channels tapering to the flow cross-sectional constriction doubled in the pipetting opening.
  • the entire length of the duct can be used as the characteristic length.
  • the diameter can be used for circular flow cross-sections, an edge length for square flow cross-sections, an arithmetic mean of long and short edge lengths for rectangular flow cross-sections, and an arithmetic mean of long and short axes, etc. for elliptical flow cross-sections. If the flow cross section changes over the length of the flow cross section constriction, the smallest flow cross section occurring in the flow cross section constriction should be used.
  • ⁇ Pof is the dynamic viscosity of a metering liquid flowing through the pipetting opening
  • ⁇ Dst is a dynamic viscosity of a working fluid flowing through the throttling point
  • I Pof is a characteristic length of the pipetting opening
  • I Dst is a characteristic length of the throttling point
  • d Pof is a characteristic dimension of the flow cross section of the pipetting opening
  • dost is a characteristic dimension of the flow cross section of the throttle point.
  • the present invention also relates to a washing head pipetting device already mentioned at the outset, which is designed to dispense washing liquid as metering liquid in precise dosages.
  • washing head pipetting devices are generally used to clean objects in sample containers, such as so-called "wells", by dispensing a precisely metered amount of washing liquid.
  • the exact dosage of the washing liquid is of great importance in order to bring about a predetermined cleaning state.
  • the general rule is that the volume flow of washing liquid is set so that the washing performance is as large as possible, but the duration of the washing process is as short as possible. In the event of incorrect pipetting of washing liquid, there can be a risk of a so-called “overwashing”, which can lead to undesired detachment of elements on the object to be washed and / or in the sample container.
  • washing head pipetting device aspirates the washing liquid as dosing liquid via the pipetting opening into the dosing liquid receiving space
  • washing liquid is supplied to the dosing liquid receiving space of a preferred washing head pipetting device through a separate washing liquid feed for reasons of simpler handling.
  • the pipetting device discussed here can have a washing liquid inlet, through which the dosing liquid receiving space can be at least partially filled with washing liquid.
  • the washing liquid inlet opens into the metering liquid receiving space.
  • the washing liquid inlet is generally provided as a channel formed separately from the metering liquid receiving space.
  • the pipetting device can have a washing liquid pump for operating the washing liquid supply, with which washing liquid can be conveyed along the washing liquid supply into the dosing liquid receiving space.
  • a valve can be provided on the washing liquid inlet, in particular in a region near the mouth, which can be opened and closed by a control device.
  • the washing liquid pump can also be operated with this or another control device.
  • the above-mentioned washing liquid pump does not have to be provided, however, since the washing liquid can be conveyed by gravity from a geodetically above the mouth of the washing liquid inlet into the metering liquid receiving space. Then the valve mentioned above is absolutely necessary.
  • This can have a plurality of pipetting channels, which are provided essentially parallel to one another, so that a plurality of objects corresponding to the plurality of pipetting channels can be subjected to cleaning by the pipetting device at the same time.
  • the present invention is particularly advantageous in the case of a multi-channel pipetting device, since the invention can ensure that each pipetting channel can dispense essentially the same amount of metering liquid with high accuracy, even though the individual pipetting channels, be it due to manufacturing tolerances of coupled pipetting tips, be it due to deposits of different thicknesses at the pipetting openings, or a combination of these or other causes, can have different geometric shapes, so that the individual pipetting channels of a multichannel pipetting head can be used without using the present invention the same operating parameters of the pipetting device would deliver different pipetting results.
  • the principle of throttling the working fluid flow between the metering liquid receiving space and the pressure change device can thus be successfully used not only in the dispensing of metering liquids, but also in their aspiration.
  • the dosing behavior may become insensitive to deposits and other changes in the flow cross section in the pipetting opening.
  • the present invention therefore relates to pipetting devices which, in addition to dispensing, are also used for Aspiration of dosing liquid, in this case by reducing the pressure of the working fluid in the dosing liquid receiving space, are formed.
  • dosing liquid When dosing liquid is aspirated, it can be aspirated into the dosing liquid receiving space depending on the pressure of the working fluid through the pipetting opening.
  • the throttle point in the pipetting channel that is recommended in the present case is fluid-mechanically suitable between the pressure changing device and the metering liquid receiving space within certain limits for evening out the metering behavior across differently viscous metering liquids.
  • the dosing behavior of these pipetting devices is within certain limits independent of the viscosity of the dosing liquid.
  • the throttle point can also be considered to equip the throttle point with an optionally adjustable flow cross-section, for example by changing the gap width of an annular gap or with a mechanism similar to that used to adjust the orifice on mechanical cameras.
  • the flow cross section of the throttle point can thus be adapted to the working fluid used in each case and / or the metering liquid to be metered in each case.
  • the pipetting channel is between a blocking position in which a working fluid flow in the pipetting channel is prevented, and an open position, in which a working fluid flow in the pipetting channel is permitted, has an adjustable valve.
  • the valve can initially be kept closed until the working fluid has been brought to a desired pressure in an area located at least near the pressure changing device.
  • the throttle point can be changed up to a cross-section of zero, so that the valve described here can be implemented using an advantageously small number of components by means of the throttle point described above with a variable flow cross-section.
  • a quantity of liquid can be metered with high precision in a manner known per se by intermittently opening and closing the valve.
  • At least one reservoir of working fluid under a system pressure can be provided as the pressure changing device. More precisely, in order to carry out both aspiration and dispensing processes on one and the same pipetting channel, a dispensing reservoir under a first system pressure and an aspiration reservoir under a second system pressure can be provided, which can optionally be connected to the pipetting channel in a pressure-transmitting manner and are separable therefrom, the first system pressure being greater than an ambient pressure of the pipetting device and the second system pressure being less than the ambient pressure.
  • the system pressure at least for dispensing processes, is particularly preferably overpressure of 1.5 bar, preferably 1.2 bar, compared to the ambient pressure of the metering liquid receiving space of 1.0 bar, does not exceed.
  • the system pressure at least for dispensing processes, is particularly preferably overpressure of 1.5 bar, preferably 1.2 bar, compared to the ambient pressure of the metering liquid receiving space of 1.0 bar, does not exceed.
  • the pressure change device has a discontinuously or continuously operating pump, possibly in combination with a valve arrangement, which can be arranged in the delivery path of the pump and optionally opened and closed.
  • a valve arrangement which can be arranged in the delivery path of the pump and optionally opened and closed.
  • the pressure changing device has a piston-cylinder device with a cylinder extending along a cylinder axis and with a piston movably received therein along the cylinder axis, the cylinder and piston limiting at least one working volume which can be changed by a relative movement of the piston relative to the cylinder and which is or can be brought into fluid transmission connection with the pipetting channel.
  • the piston-cylinder arrangement represents the most common configuration of the pressure changing device in pipetting devices. It also offers the possibility of very precise pressure control through the use of small piston areas and long piston strokes in relation to it.
  • the pipetting device can also be a pipetting device which can be operated manually as intended, in which case the piston can be moved relative to the cylinder by manual operation. This actuation can take place directly, that is to say by pulling out or pushing in the piston by hand, or indirectly, for example by tensioning a spring which drives a relative movement between the piston and cylinder after triggering.
  • the manually operable pipetting device preferably has only exactly one pipetting channel for the most accurate dosing possible.
  • FIG. 1 is a roughly illustrated embodiment of a pipetting device according to the invention, generally designated 10.
  • the pipetting device 10 comprises a pipetting channel 12, to which a pipetting tip 14 is detachably coupled in a manner known per se.
  • the pipetting channel 12 has a cylinder section 16 in which a piston 18 can be adjusted relative to the cylinder section 16 along a longitudinal axis L of the pipetting device 10 coinciding with the cylinder axis Z via a piston rod 20.
  • the motor 22 is controlled by a control unit 24, for example in dependence on the detection signal of a pressure sensor 26, which detects the pressure of a working fluid, such as air, in a working chamber 28 that changes in volume due to the movement of the piston 18.
  • a control unit 24 for example in dependence on the detection signal of a pressure sensor 26, which detects the pressure of a working fluid, such as air, in a working chamber 28 that changes in volume due to the movement of the piston 18.
  • the assembly of cylinder 16 and piston 18 forms a pressure change device 40 for changing the pressure of working fluid in the metering liquid receiving space 38.
  • the throttle point 42 is essentially formed by a cylindrical channel, so that the length of the channel is the characteristic length IDst of the throttle point 42.
  • the throttle point 42 can also have a valve 44, with which the throttle point 42 can be completely closed, in order to interrupt a pressure spread of the working fluid pressure from the working space 28 into the metering liquid receiving space 38.
  • the valve 44 is preferably also operable by the control device 24.
  • the characteristic length I is the length which the outlet end of the pipette tip 14 has from the pipette opening 36 to the point at which the flow cross section of the pipette tip 14 has twice the area as the pipette opening 36.
  • the flow cross section in a circular shape is proportional to the square of the Radius or the diameter can be a characteristic length of a tapered or otherwise as to the respective flow cross-section constriction with the narrowest flow cross-section tapering outlet area, the length is assumed to exist between the respective flow cross-section constriction and a flow cross-section, the diameter of which ⁇ 2 times the diameter of the flow cross-section constriction.
  • the ratio of the two flow resistances at the throttling point 42 and the pipetting opening 36 does not exceed a ratio of 0.5, preferably 0.3, particularly preferably 0.225, this is Dispensing behavior of the pipette tip 14, which can also be rigidly connected to the pipette channel 12, largely independently of changes in the flow cross section, for example due to deposits of dried or / and crystallized metering liquid.
  • the metering behavior changes with increasing narrowing of the pipetting opening 36 from falling below a critical narrowing degree, even in spite of the provision of the throttle point 42 in the pipetting channel 12, between the pressure changing device 40 and the metering liquid receiving space 38.
  • the limit beyond which influences of such deposits can occur of the pipetting opening 36 or in a region close to the pipetting opening 36 during dispensing are pushed out further in the direction of a cross-sectional reduction of the pipetting opening 36.
  • the ratio of the flow cross sections R 2 to R 1 is less than 0.001, preferably less than 0.00075 and particularly preferably less than 0.0005, the aspiration and dispensing behavior of the pipetting device can even be within certain limits independent of the viscosity of the Dosing liquid used are made so that different viscous dosing liquids can be dosed the same with one and the same pipetting device 10 and one and the same operating parameters. This considerably simplifies the operation of pipetting devices.
  • the present invention is also applicable for dosing tasks which a pipetting device 10 has to fulfill as a so-called “washing head pipetting device” if this washing liquid is to be taken up and dispensed in precise doses, for example as a metering liquid.
  • Such pipetting devices can be used to clean objects 37 in sample containers 39 or sample containers 39 alone, which are usually located under the pipetting opening 36, by dispensing a measured amount of washing liquid as the metering liquid.
  • a pipetting device 10 can also have a washing liquid inlet 50, which, starting from a washing liquid supply 52, can open into the metering liquid receiving space 38 at an opening 54.
  • the dosing liquid receiving space can advantageously be filled with washing liquid through the washing liquid inlet 50, so that the washing liquid need not be aspirated through the pipetting opening 36 in this case.
  • the washing liquid in the washing liquid supply 52 can be conveyed into the metering liquid receiving space 38 by a pump 56, which can also be controlled by the control / regulating device 24, via the washing liquid inlet 50.
  • a valve 58 can also be provided on the washing liquid inlet 50, which valve 58 can be opened and closed by the control device 24.
  • a predetermined pressure can be built up in the washing liquid inlet 50 when the valve 58 is closed by operating the pump 56, whereupon the valve 58 is opened for a predetermined time and then closed again.
  • the valve 58 is preferably at the mouth 54 or in relation to the total length of the washing liquid inlet close to the mouth 54 arranged.
  • the distance of the valve 58 from the mouth 54 should preferably not exceed 5% of the total length of the washing liquid inlet 58.
  • the pipetting device 10 can have, in addition to the pipetting channel 12 shown, further pipetting channels which are essentially identical to the pipetting channel 12 shown, so that the pipetting device 12 shown in FIG Fig. 1
  • the washing liquid inlets 50 to each pipetting channel 12 can be connected to a common washing liquid supply 52 via a common pump 56.
  • all piston rods 20 of the individual pipetting channels 12 can be adjusted by a common motor 22.
  • each pipetting channel has its own motor 22, its own pump 56 and / or its own washing liquid supply 52.
  • the fixing means 60 is intended to indicate that the pipette tip 34 can be permanently coupled to the pipette channel 12 as a washing tube and cannot be detached.
  • the washing tube can also be formed in one piece with a tube of the pipetting channel, for example with the cylinder section 16.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Claims (14)

  1. Dispositif de pipetage (10) pour distribuer et aspirer du liquide de dosage en augmentant ou en diminuant la pression d'un fluide de travail, comprenant :
    - un espace de réception (38) pour le liquide de dosage à remplir au moins partiellement avec le fluide de travail, comprenant une ouverture de pipetage (36) comme premier rétrécissement de section d'écoulement à travers laquelle le liquide de dosage peut être distribué et aspiré depuis l'espace de réception de liquide de dosage (38) en fonction de la pression du fluide de travail, et
    - un dispositif de changement de pression (40) qui est conçu pour modifier la pression du fluide de travail dans l'espace de réception de liquide de dosage (38),
    - dans un canal de pipetage (12) rempli fonctionnellement de fluide de travail, en communication fluidique entre l'espace de réception de liquide de dosage (38) et le dispositif de modification de pression (40), un point d'étranglement (42) comme autre rétrécissement de section d'écoulement, le rapport entre la résistance d'écoulement (R1) de l'ouverture de pipetage (36) en ce qui concerne un liquide de dosage dont la viscosité ne dépasse pas la valeur de 0,004 Nsm-2 et la résistance d'écoulement (R2) du point d'étranglement (42) en ce qui concerne un fluide de travail dont la viscosité ne dépasse pas la valeur de 0,00003 Nsm-2, est inférieur à 0,5,
    caractérisé en ce que
    - le canal de pipetage (12) est le lien d'aspiration et de distribution entre l'espace de réception de liquide de dosage (38) et le dispositif de changement de pression (40).
  2. Dispositif de pipetage selon la revendication 1, caractérisé en ce que le rapport est inférieur à 0,3, de préférence inférieur à 0,225.
  3. Dispositif de pipetage (10) selon la revendication 1 ou 2, caractérisé en ce qu'il s'agit d'un dispositif de pipetage à tête de lavage (10) qui est conçu pour distribuer un liquide de lavage et qui présente une alimentation de liquide de lavage (50) qui débouche de préférence dans l'espace de réception de liquide de dosage (38), de sorte que l'espace de réception de liquide de dosage (38) peut être rempli par l'alimentation de liquide de lavage (50) au moins partiellement avec du liquide de lavage.
  4. Dispositif de pipetage (10) selon l'une des revendications 1 à 3, caractérisé en ce qu'il présente une pluralité de canaux de pipetage (12) essentiellement parallèles, chaque canal de pipetage (12) étant pourvu d'un point d'étranglement (42) comme autre rétrécissement de section d'écoulement.
  5. Dispositif de pipetage (10) selon l'une des revendications précédentes, caractérisé en ce que le rapport est inférieur à 0,00075, de préférence inférieur à 0,0005.
  6. Dispositif de pipetage (10) selon l'une des revendications précédentes, caractérisé en ce que le point d'étranglement (42) présente une section d'écoulement pouvant être modifiée de façon sélective.
  7. Dispositif de pipetage (10) selon l'une des revendications précédentes, caractérisé en ce que le canal de pipetage (12) présente une soupape (44) pouvant être réglée entre une position fermée, dans laquelle on empêche le flux du fluide de travail dans le canal de pipetage (12), et une position ouverte, dans laquelle on autorise le flux du fluide de travail dans le canal de pipetage (12).
  8. Dispositif de pipetage (10) selon la revendication 7, caractérisé en ce que la soupape (44) est prévue au niveau du point d'étranglement (42) ou bien en communication fluidique entre le dispositif de changement de pression (40) et le point d'étranglement (42).
  9. Dispositif de pipetage (10) selon l'une des revendications précédentes, caractérisé en ce qu'il présente comme dispositif de changement de pression (40) au moins un réservoir de fluide de travail sous pression.
  10. Dispositif de pipetage (10) selon la revendication 9, caractérisé en ce qu'il est prévu un réservoir de distribution se trouvant sous une première pression et un réservoir d'aspiration se trouvant sous une deuxième pression, lesquels peuvent être au choix raccordés par transmission de pression au canal de pipetage (12) ou en être détachés, la première pression étant supérieure à une pression ambiante du dispositif de pipetage (10) et la deuxième pression étant inférieure à la pression ambiante.
  11. Dispositif de pipetage (10) selon la revendication 9 ou 10, caractérisé en ce que la pression pour les processus de distribution par rapport à la pression ambiante de l'espace de réception de liquide de dosage (38) n'excède pas une surpression de 1,5 bar, de préférence de 1,2 bar, de façon particulièrement préférée de 1,0 bar.
  12. Dispositif de pipetage (10) selon l'une des revendications précédentes, caractérisé en ce que l'espace de réception de liquide de dosage (38) et l'ouverture de pipetage (36) sont réalisés sur un embout de pipetage (14) réalisé séparé du canal de pipetage (12) présentant le point d'étranglement (42), au choix de façon à pouvoir être raccordé ou séparé de ce dernier.
  13. Dispositif de pipetage (10) selon l'une des revendications précédentes avec un liquide de dosage dont la viscosité ne dépasse pas la valeur de 0,004 Nsm-2, de préférence la valeur de 0,0035 Nsm-2, de façon particulièrement préférée la valeur de 0,0031 Nsm-2.
  14. Dispositif de pipetage (10) selon l'une des revendications précédentes avec un fluide de travail dont la viscosité ne dépasse pas la valeur de 0,00003 Nsm-2, de préférence la valeur de 0,00002 Nsm-2, de façon particulièrement préférée la valeur de 0,0000175 Nsm-2.
EP11172869.7A 2010-07-26 2011-07-06 Dispositif de pipetage doté d'un poste d'étranglement dans le canal de pipetage Active EP2412439B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP20152812.2A EP3663001B1 (fr) 2010-07-26 2011-07-06 Methode de pipetage pourvu de point d'étranglement dans le canal de pipetage
JP2011161908A JP2012068231A (ja) 2010-07-26 2011-07-25 ピペット管内の絞り点を有するピペット装置
US13/190,686 US8656792B2 (en) 2010-07-26 2011-07-26 Pipette device having a throttle point in the pipette duct
JP2017001365A JP6463386B2 (ja) 2010-07-26 2017-01-06 ピペット管内の絞り点を有するピペット装置

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102010038414A DE102010038414A1 (de) 2010-07-26 2010-07-26 Pipettiervorrichtung mit Drosselstelle im Pipettierkanal

Related Child Applications (2)

Application Number Title Priority Date Filing Date
EP20152812.2A Division-Into EP3663001B1 (fr) 2010-07-26 2011-07-06 Methode de pipetage pourvu de point d'étranglement dans le canal de pipetage
EP20152812.2A Division EP3663001B1 (fr) 2010-07-26 2011-07-06 Methode de pipetage pourvu de point d'étranglement dans le canal de pipetage

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Publication Number Publication Date
EP2412439A1 EP2412439A1 (fr) 2012-02-01
EP2412439B1 true EP2412439B1 (fr) 2020-03-04

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EP20152812.2A Active EP3663001B1 (fr) 2010-07-26 2011-07-06 Methode de pipetage pourvu de point d'étranglement dans le canal de pipetage
EP11172869.7A Active EP2412439B1 (fr) 2010-07-26 2011-07-06 Dispositif de pipetage doté d'un poste d'étranglement dans le canal de pipetage

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EP20152812.2A Active EP3663001B1 (fr) 2010-07-26 2011-07-06 Methode de pipetage pourvu de point d'étranglement dans le canal de pipetage

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US (1) US8656792B2 (fr)
EP (2) EP3663001B1 (fr)
JP (2) JP2012068231A (fr)
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Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010084208A2 (fr) 2010-05-14 2010-07-29 Sias Ag Agencement de pipetage et procédé de commande d'un agencement de pipetage ou de production de doses de produits liquides i
US9555407B2 (en) * 2013-03-14 2017-01-31 Protedyne Corporation Laser triangulation for pipette tip position
US9519002B2 (en) * 2013-04-11 2016-12-13 Rarecyte, Inc. Device, system, and method for selecting a target analyte
US9915674B2 (en) 2013-10-17 2018-03-13 Siemens Healthcare Diagnostics Inc. Methods and apparatus for measuring aspiration pressure
DE102015104791B4 (de) * 2015-03-27 2020-01-30 Marco Systemanalyse Und Entwicklung Gmbh Dosieraggregat
WO2018091075A1 (fr) * 2016-11-15 2018-05-24 Tecan Schweiz Ag Procédé et dispositif de pipetage
WO2019096993A1 (fr) * 2017-11-17 2019-05-23 Eppendorf Ag Dispositif de microdosage pour le dosage de micro-échantillons de fluide
CN110404597A (zh) * 2019-08-08 2019-11-05 天津药明康德新药开发有限公司 一种多功能自清洁移液装置及其使用方法
EP3838411A1 (fr) 2019-12-18 2021-06-23 TECAN Trading AG Dispositif et procédé de pipetage
WO2021163437A1 (fr) 2020-02-14 2021-08-19 DeNovix, Inc. Pipette à plage volumétrique étendue dynamique
US20230101709A1 (en) * 2021-09-30 2023-03-30 Artel, Inc. Pipettable replacement fluid for mimicking pipettability of target fluid and related method

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2523809A (en) * 1949-05-26 1950-09-26 Ritter Co Inc Syringe
US3290946A (en) * 1964-05-25 1966-12-13 Dow Chemical Co Pipetting device
FI46688C (fi) 1971-06-23 1973-06-11 Suovaniemi Pipetti
GB1470515A (en) 1973-03-30 1977-04-14 Flow Labor Ltd Micro pipetting apparatus
FI58875C (fi) * 1979-08-31 1981-05-11 Suovaniemi Finnpipette Pipett
JP2513359B2 (ja) * 1990-11-30 1996-07-03 株式会社島津製作所 マイクロピペット装置
FI86812C (fi) * 1991-01-07 1992-10-26 Labsystems Oy Foertraengningspipett
FI96666C (fi) * 1992-05-04 1996-08-12 Wallac Oy Menetelmä ja ilmamäntäpipetti nesteen annostelemiseksi
FI101864B1 (fi) * 1995-07-07 1998-09-15 Biohit Oy Menetelmä nesteannosteluvirheen korjaamiseksi, ja nesteannostelulaite
JPH09127134A (ja) * 1995-11-06 1997-05-16 Sanyo Electric Co Ltd 分注装置の分注用ヘッド
US6370942B1 (en) * 2000-05-15 2002-04-16 Dade Behring Inc. Method for verifying the integrity of a fluid transfer
DE10049507B4 (de) * 2000-10-06 2009-08-20 Vitlab Gmbh Pipette
JP2002181839A (ja) * 2000-12-12 2002-06-26 Olympus Optical Co Ltd 液体分注装置およびマイクロアレイ製造装置
DE10118875C1 (de) * 2001-04-18 2002-09-12 Eppendorf Ag Verfahren zum kontrollierten Dosieren von Flüssigkeiten unter Verdrängung eines Gaspolsters
JP4036053B2 (ja) * 2002-07-31 2008-01-23 日本パルスモーター株式会社 分注装置等におけるシリンダユニット、およびその吸入・吐出量調整システム
US7155990B2 (en) * 2004-12-27 2007-01-02 Halliburton Energy Services, Inc. Method and apparatus for determining a downhole fluid sample volume
CH712572B1 (de) * 2007-11-28 2017-12-15 Integra Biosciences Ag Handpipettiergerät.
DE102008058213A1 (de) * 2008-11-19 2010-05-20 B. Braun Melsungen Ag Anästhesiespritze mit einem längsverschieblichen Vorschubkolben sowie Rückschlagventil mit Durchlass und Sperrrichtung und Verwendung eines O-Rings als Rückschlagventil
WO2010060448A1 (fr) * 2008-11-28 2010-06-03 Hamilton Bonaduz Ag Dispositif et procédé de dosage appropriés pour le dosage de très petits volumes de dosage
US8715574B2 (en) * 2009-06-19 2014-05-06 Abbott Laboratories System for managing inventory of bulk liquids

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
None *

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US20120017704A1 (en) 2012-01-26
US8656792B2 (en) 2014-02-25
EP2412439A1 (fr) 2012-02-01
JP2017070950A (ja) 2017-04-13
JP6463386B2 (ja) 2019-01-30
EP3663001A1 (fr) 2020-06-10
JP2012068231A (ja) 2012-04-05
DE102010038414A1 (de) 2012-01-26
EP3663001B1 (fr) 2022-10-19

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