EP3501657B1 - Verfahren zur reinigung von testprobenvertiefungen und reinigungskopf zur durchführung dieses verfahrens - Google Patents

Verfahren zur reinigung von testprobenvertiefungen und reinigungskopf zur durchführung dieses verfahrens Download PDF

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Publication number
EP3501657B1
EP3501657B1 EP19157296.5A EP19157296A EP3501657B1 EP 3501657 B1 EP3501657 B1 EP 3501657B1 EP 19157296 A EP19157296 A EP 19157296A EP 3501657 B1 EP3501657 B1 EP 3501657B1
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EP
European Patent Office
Prior art keywords
cleaning
tube
free end
suction
test card
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EP19157296.5A
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English (en)
French (fr)
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EP3501657A1 (de
Inventor
Frédéric FOUCAULT
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Biomerieux SA
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Biomerieux SA
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Publication of EP3501657A1 publication Critical patent/EP3501657A1/de
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/04—Cleaning involving contact with liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B7/00—Cleaning by methods not provided for in a single other subclass or a single group in this subclass
    • B08B7/04—Cleaning by methods not provided for in a single other subclass or a single group in this subclass by a combination of operations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L13/00—Cleaning or rinsing apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L13/00—Cleaning or rinsing apparatus
    • B01L13/02—Cleaning or rinsing apparatus for receptacle or instruments
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B5/00—Cleaning by methods involving the use of air flow or gas flow
    • B08B5/04—Cleaning by suction, with or without auxiliary action
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1004—Cleaning sample transfer devices
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S134/00—Cleaning and liquid contact with solids
    • Y10S134/902—Semiconductor wafer

Definitions

  • the present invention relates to the technical field of microfluidic systems in the general sense and it relates more particularly to the field of test cards for analyzes in which are arranged fluid supply channels up to wells adapted to receive a reagent and a fluid or test sample containing analytes to be detected, representative of disease or the presence of microorganisms.
  • the object of the invention relates more precisely to the technical means suitable for cleaning the wells of such test cards.
  • the patent US 4,635,665 describes a cleaning device comprising at least one washing head with two coaxial tubes intended to be introduced inside the well to be cleaned in order to extend near the bottom of the well.
  • the cleaning head comprises a cleaning tube delivering at its free end a cleaning fluid and a suction tube mounted concentrically inside the cleaning tube and adapted to suck the soiled cleaning fluid.
  • the adjacent ends of the suction tube and the cleaning tube are located in the same plane to ensure efficient suction.
  • the injection of the cleaning liquid in the center of the head and the suction of the contaminated liquid at the periphery of the head do not make it possible to guarantee total recovery of the contaminated liquid.
  • Fluid is introduced into and drawn from the substantially closed space such that the object is movable in the fluid between the end portion and the receptacle.
  • heads and material removal devices for non-invasively removing material from the wells of multi-well plates.
  • the patent US 5,882,597 describes a cleaning device comprising a series of cleaning heads each comprising a cleaning tube communicating with a storage chamber for a cleaning fluid and delivering, at its free end, a cleaning fluid.
  • Each cleaning head also has a suction tube mounted inside each cleaning tube to suck the soiled cleaning fluid from its free end which protrudes from the free end of the cleaning tube.
  • the end part of the cleaning tube is open to ensure the exit of the liquid in an annular form in order to ensure tightness preventing air from rising into the storage chamber.
  • the cleaning head is moved in order to penetrate inside the well to be cleaned.
  • the present invention therefore aims to remedy the drawbacks of the state of the art by proposing a new technique for cleaning the wells of a test card for analyzes, suitable for completely and reproducibly cleaning the test sample wells of a test card for analysis.
  • the object of the invention is to provide a method as defined in one of claims 1 to 7.
  • Another object of the invention is to provide a cleaning apparatus as defined in one of claims 8 to 13.
  • the Fig. 1 illustrates by way of example a cleaning apparatus 1 for at least one test sample well 2 arranged in a test card for analyzes 3.
  • the apparatus 1 according to the invention comprises at least one cleaning head 5 suitable for cleaning a 2 in test sample wells one or more positions depending on the shape of the well, for example in the case of an oblong-shaped well.
  • the apparatus 1 comprises a single cleaning head 5.
  • the cleaning apparatus 1 can comprise a plurality of cleaning heads 5 arranged for example side by side in rows and columns, in number and in distribution which may or may not be identical to the test sample wells 2 to be cleaned, arranged on the test card for analyzes 3.
  • Each cleaning head 5 comprises a cleaning tube 7 having, for example, a circular cross section.
  • the cleaning tube 7 comprises an outer tubular wall 8 and an inner tubular wall 9.
  • the cleaning tube 7 has a free end 71 delimited by a transverse face 10 of annular shape providing the junction between the outer wall 8 and the inner wall 9.
  • the external diameter of the cleaning tube 7 is between 0.5 mm and 25 mm and preferably between 0.5 mm and 5 mm.
  • the free end 71 of the cleaning tube 7 can have different shapes.
  • the inner wall 9 of the cleaning tube 7 has a constant section over its entire length while the outer wall 8 of the cleaning tube 7 has a constant section except for its free end 71 which has a decreasing section up to the transverse face 10.
  • the Fig. 3 illustrates another variant embodiment according to which the section of the inner wall 9 of the cleaning tube 7 is constant except for its free end which decreases to the transverse face 10.
  • the cleaning tube 7 has an end 72 opposite the free end 71 and communicating with a source of cleaning fluid 11.
  • the cleaning fluid used is water with or without adjuvants, an aqueous solvent. and / or organic, a solvent containing proteins, a washing buffer which may contain HEPES, sodium chloride, BSA (bovine serum albumin), casein, PEG (Polyethylene glycol), sodium azide, surfactants like Tween® 20, sugar, etc.
  • the cleaning fluid can also include fillers such as talc, particles, corundum, alumina, glass, in order to add mechanical action to the cleaning.
  • the cleaning head 5 also includes a suction tube 14 mounted inside the cleaning tube 7 and having a preferably circular cross section.
  • the external diameter of the suction tube 14 is between 0.2 mm and 20 mm and preferably between 0.2 mm and 4 mm.
  • the suction tube 14 comprises an outer tubular wall 15 and an inner tubular wall 16.
  • the suction tube 14 defines between its outer wall 15 and the inner wall 9 of the cleaning tube 7, a tubular duct 18 for conveying the fluid. cleaning fluid up to the free end 71 of the cleaning tube 7.
  • the suction 14 and cleaning 7 tubes are mounted coaxially.
  • the suction tube 14 has a free end 141 extending near the free end 71 of the cleaning tube 7.
  • the free end 141 of the suction tube 14 is delimited by a transverse face 19 connecting between the inner wall 16 and the outer wall 15 of the suction tube 14.
  • the suction tube 14 thus defines by its inner wall 16 a duct 20 communicating from the end 142 opposite the free end 141, with a source suction 21 of the contaminated cleaning fluid, sucked from its free end 141.
  • the suction source 21 is produced by a vacuum pump, a venturi or the network vacuum.
  • the flow rate of the suction source 21 is greater than the flow rate of the source of cleaning fluid 11.
  • the differential between the flow rates of the source of cleaning fluid 11 and of the suction source 21 depends on in particular of the operating conditions and the geometry of the cleaning head 5 and of the test sample wells 2 to be cleaned.
  • the ratio of the flow rate of the suction source to the flow rate of the source of cleaning fluid 11 is between 300 and 3000.
  • the cleaning apparatus 1 also comprises a system 25 for relative movement between the cleaning head 5 and the test card for analyzes 3 so that the cleaning head 5 occupies at least a first so-called rest position in which the cleaning head is moved away from the test card for analyzes 3 and a cleaning position for which the cleaning tube 7 ensures the supply of the cleaning fluid inside the well and the suction tube 14 aspirates the contaminated cleaning fluid.
  • the displacement system 25, which can be of any type known per se, is not described more precisely because it is well known to those skilled in the art and does not specifically form part of the invention.
  • the displacement system 25 is controlled so as to ensure, for the cleaning position, the support of the cleaning tube 7 by its transverse face 10 on the test card for analyzes 3 while the free end of the aspiration tube 14 is located above the bottom 21 of the test sample well 2. In this cleaning position, the cleaning tube partially or completely overhangs the test sample well 2. In this cleaning position, the free end and more precisely the transverse face 19 of the suction tube 14 is located at a determined distance from the bottom of the well to be cleaned 2 in order to define with the latter, a suction zone 27.
  • the transverse face 19 of the suction tube 14 is located at a distance from the bottom of the well to be cleaned 2 which can range up to 2 mm for microwells or even up to 10 mm for microplates.
  • the free end 141 of the suction tube 14 is set back relative to the free end 71 of the cleaning tube 7.
  • the transverse face 19 of the suction tube 14 is set back relative to the transverse face 10 of the cleaning tube 7.
  • the transverse face 19 of the suction tube 14 is set back relative to the transverse face 10 of the cleaning tube by a value between 10 ⁇ m and 3 mm and preferably for a microplate washing application, for example, between 1 mm and 3 mm and more preferably, for a microwell washing application between 10 ⁇ m and 300 ⁇ m.
  • the cleaning tube 7 can be provided with an air inlet 31 between the outer wall 8 and the inner wall 9.
  • the air inlet 31 comprises a series of channels 32 arranged on the transverse face 10 delimiting the free end of the cleaning tube 7. These channels 32 extend radially between the outer wall 8 and the inner wall 9 of the tube cleaning by opening onto the outer 8 and inner walls 9.
  • the channels 32 open out or open onto the tubular duct 18 for conveying the cleaning fluid, which is delimited by the inner wall 9 of the cleaning tube 7.
  • the channels 32 are distributed evenly on the transverse face 10 of the cleaning tube 7.
  • the cleaning tube 7 has a rough transverse face 10 delimiting the air inlet 31 between the outer wall 8 and the inner wall 9 of the cleaning tube 7.
  • the roughness of the transverse face 10 is such that the latter has projecting parts between them defining passages together forming the air inlet 31 between the outer 8 and inner 9 walls.
  • the air inlet 31 can also be produced by holes arranged radially at the free end 71 of the cleaning tube 7 near the transverse face 10 and opening out. on the outer wall 8 and the inner wall 9.
  • the method for cleaning a test sample well 2 therefore consists in ensuring the relative displacement between the cleaning head 5 and the test card for analyzes 3 so as to place the cleaning head 5 in its cleaning position.
  • the cleaning tube 7 In this position, shown in Fig. 1 , the cleaning tube 7 is supported by its free end 71 on the test card. In this position, the transverse face 10 of the cleaning tube 7 rests on at least part of the periphery of the test sample well 2. In this position, the cleaning tube 7 is able to inject the cleaning fluid. inside the well 2 while the suction tube 14 is overhanging the well 2 to suck the soiled cleaning fluid.
  • the injection of the cleaning fluid inside the tube 7 leads to its exit through the free end 71 of the cleaning tube 7. Simultaneously, the suction source 21 sucks from the free end 141 of the tube d. suction 14 the contaminated liquid. Due to the air inlet 31 made between the outside and the inside of the cleaning tube 7, there occurs inside the test sample well 2, a vortex of the cleaning fluid allowing efficient cleaning. and complete with the test sample well 2. Since the suction tube 14 is located inside the cleaning tube 7, there is good recovery of the contaminated cleaning fluid.
  • the positioning of the free end 141 of the suction tube 14 relative to the free end 71 of the cleaning tube 7 depends in particular on the depth of the test sample well 2 to be cleaned. As indicated above, the free end 141 of the suction tube 14 is advantageously placed back relative to the free end 71 of the cleaning tube 7.
  • the transverse face 19 of the suction tube 14 is located at a distance from the bottom of the well to be cleaned 2 which can range up to 2 mm for microwells or even up to 10 mm for microwells. microplates.
  • the air inlet 31 between the outside and the inside of the free end 71 of the cleaning tube 7 is produced by the geometry of the cleaning tube 7.
  • the Fig. 6 illustrates another exemplary embodiment in which the air inlet 31 is produced by the geometry of the test card for analyzes 3.
  • the test sample well 2 communicates with at least one and in the example illustrated in Fig. 6 , two conduits 34 arranged in the test card for analyzes 3 and forming the air inlet 31.
  • These conduits 34 are arranged so that in the position of support of the free end 71 of the cleaning tube 7 on the card test for analyzes 3, the conduits 34 extend on either side of the inner 9 and outer 8 walls to allow the entry of air inside the cleaning tube 7. In this bearing position, the cleaning tube. cleaning 7 completely surrounds or closes well 2, with the exception of conduits 34.
  • Cleaning tube 7 seals between cleaning head 5 and test card for analyzes 3.
  • the free end 71 of the cleaning tube 7 is suitable for sealing with the test card for analyzes 3.
  • the cleaning tube 7 is provided at its free end 71, a flexible tip ensuring tightness with the test card. Such a flexible tip also makes it possible to obtain a soft contact between the cleaning tube 7 and the test card for analyzes 3, and to compensate for the tolerances of the displacement system 25 to ensure the positioning of the cleaning head 5 in its position of. cleaning.
  • Such a flexible tip can be added to the free end 71 of the cleaning tube or be overmolded or co-injected at the level of the free end 71 of the cleaning tube 7.
  • the flexible tip can have a hardness included. between 20 and 80 Shore A.
  • Such a tip can be made of silicone, elastomers such as EPDM (Ethylene Propylene Diene Monomer), SBR (Styrene Butadiene Rubber) ... or others or thermoplastic elastomers such as Arnitel®, Pebax® ... or others.
  • the suction tube 14 internally comprises a duct 20 opening at the free end 141, by a single suction section 20a, corresponding to the complete cross section of the duct 20.
  • the Fig. 7 and 8 illustrate another variant embodiment of the suction tube 14 making it possible to suck the contaminated cleaning fluid from the free end 141, in several suction sections 20a separated from each other by partitions 38. These sections d
  • the suction 20a are arranged at the free end 141 of the suction tube 14 so that in the cleaning position, these suction sections 20a are located in line with spots placed in the test sample well 2 Such an arrangement contributes to reinforcing the cleaning of the well 2 and more particularly in the areas where one or more products of interest are deposited, called spots.
  • the duct 20 of the suction tube 14 is provided at the free end 141 of the suction tube 14, with partitions 38 which are arranged in any suitable manner to form a specific geometry or pattern. These partitions 38 define the suction sections 20a which in the example illustrated in Fig. 7 are of different shapes.
  • the Fig. 8 illustrates another variant embodiment in which the suction tube 14 comprises suction sections 20a of identical shapes, namely circular for example.
  • the duct 20 of the tube suction 14 opens at the free end 141, for example, by four suction sections 20a separated from each other by the partitions 38 formed by a transverse face closing the free end 141 of the suction tube 14.
  • each suction section 20a is provided with an inlet cone 39 converging towards the conduit 20 of the suction tube 14 to facilitate the selective delivery of the soiled cleaning fluid through the suction sections 20a.
  • inlet cone 39 converging towards the conduit 20 of the suction tube 14 to facilitate the selective delivery of the soiled cleaning fluid through the suction sections 20a.
  • a relative movement is made between the cleaning head 5 and the test card for analyzes 3 in order to place the cleaning head 5 in a cleaning position. It should be noted that provision may be made to ensure the relative movement between the test card and the cleaning head so as to move the suction zone laterally to a determined height between the free end of the suction tube and the bottom of the well when the suction zone does not allow the bottom of the well to be cleaned in a single cleaning position.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Cleaning In General (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (13)

  1. Verfahren zum Reinigen mindestens einer Testprobenvertiefung (2), die in einer Testkarte für Analysen (3) eingerichtet ist, mit Hilfe mindestens eines Reinigungskopfs (5), der mindestens ein Reinigungsrohr (7), das eine Außenwand (8) und eine Innenwand (9) hat und an seinem freien Ende (71) eine Reinigungsflüssigkeit liefert, und ein Ansaugrohr (14) aufweist, das im Inneren des Reinigungsrohrs (7) montiert ist, um die verschmutzte Reinigungsflüssigkeit ausgehend von seinem freien Ende (141) anzusaugen, das sich in der Nähe des freien Endes (71) des Reinigungsrohrs befindet, wobei das Verfahren darin besteht, eine relative Verschiebung zwischen der Testkarte (3) und dem Reinigungskopf (5) zu gewährleisten, so dass die Testkarte eine Reinigungsposition einnimmt, in der das Reinigungsrohr (7) an seinem freien Ende die Reinigungsflüssigkeit ins Innere der zu reinigenden Vertiefung (2) liefert, und das Ansaugrohr (14) die verschmutzte Reinigungsflüssigkeit ansaugt, dadurch gekennzeichnet, dass es in der Reinigungsposition darin besteht, die relative Verschiebung zwischen der Testkarte (3) und dem Reinigungskopf (5) zu gewährleisten, um die Auflage des freien Endes (71) des Reinigungsrohrs (7) auf der Testkarte (3) zu gewährleisten, um einen Lufteingang (31) zwischen dem Äußeren und dem Inneren des freien Endes (71) des Reinigungsrohrs (7) zu erzeugen, um einen Wirbel der Reinigungsflüssigkeit im Inneren der zu reinigenden Vertiefung (2) zu erzeugen, und dass es darin besteht, die Testkarte (3) so einzurichten, dass die Testkarte den Lufteingang (31) erzeugt, wenn das freie Ende (71) des Reinigungsrohrs (7) auf der Testkarte in Auflage ist, und dass der Lufteingang (31) durch die Geometrie der Testkarte für Analysen (3) hergestellt wird.
  2. Verfahren nach Anspruch 1, dadurch gekennzeichnet, dass die Testprobenvertiefung (2) mit mindestens einem Kanal (34) in Verbindung steht, der in der Testkarte für Analysen (3) eingerichtet ist und den Lufteingang (31) formt, wobei dieser Kanal (34) so eingerichtet ist, dass der Kanal (34) sich in der Auflageposition des freien Endes (71) des Reinigungsrohrs (7) auf der Testkarte für Analysen (3) zu beiden Seiten der Innen- (9) und Außenwände (8) des Reinigungsrohrs (7) erstreckt, um den Lufteingang ins Innere des Reinigungsrohrs (7) zu erlauben, und dass in dieser Auflageposition des freien Endes (71) des Reinigungsrohrs (7) auf der Testkarte für Analysen (3) das Reinigungsrohr (7) die Vertiefung (2) mit Ausnahme der Kanäle (34) umgibt oder vollständig verschließt.
  3. Verfahren nach Anspruch 1 oder 2, dadurch gekennzeichnet, dass es darin besteht, das freie Ende (71) des Reinigungsrohrs (7) mit Hilfe eines biegsamen Endstücks auszustatten, das die Dichtheit mit der Testkarte gewährleistet, wenn das Endstück auf der Testkarte in Auflage ist.
  4. Verfahren nach Anspruch 1 oder 2, dadurch gekennzeichnet, dass die Auflage des freien Endes (71) des Reinigungsrohrs (7) auf der Testkarte (3) eine Ansaugzone (27) bestimmter Höhe zwischen dem freien Ende (141) des Ansaugrohrs (14) und dem Boden (21) der Vertiefung (2) erzeugt.
  5. Verfahren nach Anspruch 4, dadurch gekennzeichnet, dass es darin besteht, die Ansaugzone (27) bestimmter Höhe zwischen dem freien Ende (141) des Ansaugrohrs (14) und dem Boden (21) der Vertiefung (2) zu erzeugen, wobei das freie Ende (141) des Ansaugrohrs (14) bezüglich des freien Endes (71) des Reinigungsrohrs (7) übersteht.
  6. Verfahren nach Anspruch 4, dadurch gekennzeichnet, dass es darin besteht, die Ansaugzone (27) bestimmter Höhe zwischen dem freien Ende (141) des Ansaugrohrs (14) und dem Boden (21) der Vertiefung (2) zu erzeugen, wobei das freie Ende (141) des Ansaugrohrs (14) sich bezüglich des freien Endes (71) des Reinigungsrohr (7) zurückgezogen erstreckt.
  7. Verfahren nach einem der Ansprüche 1 bis 6, dadurch gekennzeichnet, dass es darin besteht, die verschmutzte Reinigungsflüssigkeit ausgehend vom freien Ende (141) des Ansaugrohrs (14) gemäß mehreren getrennten Ansaugquerschnitten (20a) anzusaugen, die sich in der Reinigungsposition lotrecht zu Punkten befinden, die in der Testprobenvertiefung (2) platziert sind.
  8. Reinigungsgerät, das eine Quelle von Reinigungsflüssigkeit (11), eine Ansaugquelle (21) für die verschmutzte Reinigungsflüssigkeit, mindestens einen Reinigungskopf (5) und ein System zur relativen Verschiebung (25) zwischen dem Reinigungskopf (5) und einer Testkarte für Analysen (3) aufweist, in der mindestens eine Testprobenvertiefung (2) eingerichtet ist, wobei das Verschiebesystem (25) gesteuert wird, um mindestens eine Reinigungsposition der Vertiefung einzunehmen, wobei der Reinigungskopf mindestens ein Reinigungsrohr (7), das mit der Quelle von Reinigungsflüssigkeit (11) verbunden ist und an seinem freien Ende (71) die Reinigungsflüssigkeit liefert, und ein Ansaugrohr (14) aufweist, das im Inneren des Reinigungsrohrs (7) montiert und mit der Ansaugquelle (21) verbunden ist und an seinem freien Ende (14 1), das sich in der Nähe des freien Endes (71) des Reinigungsrohrs (7) befindet, die verschmutzte Reinigungsflüssigkeit ansaugt, dadurch gekennzeichnet, dass die Testkarte (3) so eingerichtet ist, dass die Testkarte einen Lufteingang (31) erzeugt, wenn das freie Ende (71) des Reinigungsrohrs (7) auf der Testkarte in Auflage ist, und dass der Lufteingang (31) durch die Geometrie der Testkarte für Analysen (3) hergestellt wird.
  9. Reinigungsgerät nach Anspruch 8, dadurch gekennzeichnet, dass die Testprobenvertiefung (2) mit mindestens einem Kanal (34) in Verbindung steht, der in der Testkarte für Analysen (3) eingerichtet ist und den Lufteingang (31) formt, wobei dieser Kanal (34) so eingerichtet ist, dass der Kanal (34) sich in der Auflageposition des freien Endes (71) des Reinigungsrohrs (7) auf der Testkarte für Analysen (3) zu beiden Seiten der Innen- (9) und Außenwände (8) des Reinigungsrohrs (7) erstreckt, um den Lufteingang ins Innere des Reinigungsrohrs (7) zu erlauben, und dass in dieser Auflageposition des freien Endes (71) des Reinigungsrohrs (7) auf der Testkarte für Analysen (3) das Reinigungsrohr (7) die Vertiefung (2) mit Ausnahme der Kanäle (34) umgibt oder vollständig verschließt.
  10. Reinigungsgerät nach einem der Ansprüche 8 oder 9, dadurch gekennzeichnet, dass das freie Ende (141) des Ansaugrohrs (14) sich bezüglich des freien Endes (71) des Reinigungsrohrs (7) zurückversetzt befindet.
  11. Reinigungsgerät nach Anspruch 10, dadurch gekennzeichnet, dass das freie Ende (141) des Ansaugrohrs (14) sich bezüglich des freien Endes (71) des Reinigungsrohrs (7) gemäß einem Wert zwischen 10 µm und 300 µm zurückversetzt befindet.
  12. Reinigungsgerät nach einem der Ansprüche 8 bis 11, dadurch gekennzeichnet, dass das Reinigungsrohr (7) mit einem biegsamen Endstück versehen ist, das das freie Ende (71) des Reinigungsrohrs (7) formt.
  13. Reinigungsgerät nach einem der Ansprüche 8 bis 12, dadurch gekennzeichnet, dass das Ansaugrohr (14) innen einen Kanal (20) aufweist, der mit der Ansaugquelle (21) in Verbindung steht, und am freien Ende (141) mit Trennwänden (38) ausgestattet ist, die getrennte Ansaugquerschnitte (20a) begrenzen.
EP19157296.5A 2012-04-19 2013-04-17 Verfahren zur reinigung von testprobenvertiefungen und reinigungskopf zur durchführung dieses verfahrens Active EP3501657B1 (de)

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FR1253607A FR2989602B1 (fr) 2012-04-19 2012-04-19 Procede pour nettoyer des puits a echantillons de test et tete de nettoyage en faisant application
PCT/FR2013/050847 WO2013156736A1 (fr) 2012-04-19 2013-04-17 Procede pour nettoyer des puits a echantillons de test et tete de nettoyage en faisant application
EP13722495.2A EP2852463B1 (de) 2012-04-19 2013-04-17 Verfahren zur reinigung von testprobenvertiefungen und reinigungskopf zur durchführung dieses verfahrens

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US10954783B2 (en) * 2015-10-22 2021-03-23 Halliburton Energy Services, Inc. Extraction cleaner and gas system check
CN112292206A (zh) * 2018-02-05 2021-01-29 生物辐射实验室股份有限公司 具有抽吸柱的微流体探针头
FR3095139B1 (fr) * 2019-04-19 2021-10-08 Auum Dispositif de nettoyage d’un objet

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US10144039B2 (en) 2018-12-04
EP2852463B1 (de) 2019-03-13
KR102135280B1 (ko) 2020-07-17
JP6211590B2 (ja) 2017-10-11
EP3501657A1 (de) 2019-06-26
US20190076887A1 (en) 2019-03-14
FR2989602A1 (fr) 2013-10-25
CN103372547A (zh) 2013-10-30
US11020775B2 (en) 2021-06-01
EP2852463A1 (de) 2015-04-01
US20150075565A1 (en) 2015-03-19
CN203281537U (zh) 2013-11-13
WO2013156736A1 (fr) 2013-10-24
KR20150004363A (ko) 2015-01-12
JP2015514990A (ja) 2015-05-21
CN103372547B (zh) 2017-04-26
FR2989602B1 (fr) 2014-05-09

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