EP3442689A1 - Tangentialflussfilter aus dickwandigen hohlfasern - Google Patents

Tangentialflussfilter aus dickwandigen hohlfasern

Info

Publication number
EP3442689A1
EP3442689A1 EP17724140.3A EP17724140A EP3442689A1 EP 3442689 A1 EP3442689 A1 EP 3442689A1 EP 17724140 A EP17724140 A EP 17724140A EP 3442689 A1 EP3442689 A1 EP 3442689A1
Authority
EP
European Patent Office
Prior art keywords
thick wall
wall hollow
twhftff
hollow fiber
hollow fibers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP17724140.3A
Other languages
English (en)
French (fr)
Inventor
David Serway
Michael BRANSBY
Steve LE
Bao Le
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Spectrum Inc
Original Assignee
Spectrum Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US15/095,435 external-priority patent/US10711238B2/en
Application filed by Spectrum Inc filed Critical Spectrum Inc
Publication of EP3442689A1 publication Critical patent/EP3442689A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/08—Hollow fibre membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
    • B01D29/88—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor having feed or discharge devices
    • B01D29/90—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor having feed or discharge devices for feeding
    • B01D29/908—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor having feed or discharge devices for feeding provoking a tangential stream
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D39/00—Filtering material for liquid or gaseous fluids
    • B01D39/14—Other self-supporting filtering material ; Other filtering material
    • B01D39/16—Other self-supporting filtering material ; Other filtering material of organic material, e.g. synthetic fibres
    • B01D39/1692—Other shaped material, e.g. perforated or porous sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
    • B01D67/0081—After-treatment of organic or inorganic membranes
    • B01D67/0088—Physical treatment with compounds, e.g. swelling, coating or impregnation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/04—Tubular membranes
    • B01D69/043—Tubular membranes characterised by the tube diameter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/26—Polyalkenes
    • B01D71/261—Polyethylene
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2325/00—Details relating to properties of membranes
    • B01D2325/04—Characteristic thickness
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/30—Polyalkenyl halides
    • B01D71/32—Polyalkenyl halides containing fluorine atoms
    • B01D71/34—Polyvinylidene fluoride

Definitions

  • the present invention relates to filters and in particular to a thick wall tangential flow filter.
  • the present invention addresses the above and other needs by providing a Thick Wall Hollow Fiber Tangential Flow Filter (TWHFTFF) for perfusion and harvest of mammalian cell culture or cultures.
  • TWHFTFF Thick Wall Hollow Fiber Tangential Flow Filter
  • the TWHFTFF includes at least one thick wall hollow fiber reaching from an inlet to an outlet.
  • the thick wall hollow fiber including thick walls having tortuous paths between an interior surface and an exterior surface of the thick wall hollow fiber.
  • the tortuous paths include settling zones which capture small particles and narrowed zones which capture larger particles.
  • the walls may be made of HDPE or plastic materials including PES, PVDF and may be coated with PVDF.
  • a tangential flow filter including thick wall hollow fibers.
  • the thick wall hollow fibers include tortuous paths which selectively capture particles stripped from a retentate flow through the filter and a permeate from the filter.
  • FIG. 1 shows a cross-sectional view of a hollow fiber tangential flow filter according to the present invention.
  • FIG. 2 shows a cross-sectional view of a wall of a thick wall hollow fiber tangential flow filter according to the present invention.
  • FIG. 1 A cross-sectional view of the hollow fiber tangential flow filter 30 is shown in FIG. 1 .
  • the hollow fiber tangential flow filter 30 includes parallel thick wall hollow fibers 60 residing between an inlet chamber 30a and an outlet cham ber 30b.
  • An inlet port 32a provides a flow 12 to the inlet chamber 30a and an outlet port 32d receives a retentate flow 16 from the outlet chamber 30b.
  • the thick wall hollow fibers 60 receive the flow 12 through the inlet chamber 30a.
  • the flow 12 advanced through a hollow fiber interior 60a of the thick wall hollow fibers 60 and releases a permeate flow (for example, protein or waste material) 24 through thick walls 70 into a permeate section 61 of the filter housing 31 .
  • a permeate flow for example, protein or waste material
  • the permeate flow 24 travels to permeate ports 32b and 32c and is released or may be drawn from the filter housing 31 by a pump.
  • the filtered retentate flow 16 is released from the thick wall hollow fibers 60 into the outlet chamber 30b and is released from the filter 30 through retentate port 32d.
  • FIG. 2 A cross-sectional view of a thick wall 70 of the thick wall hollow fibers 60 of the thick wall hollow fiber tangential flow filter 30 is shown in FIG. 2.
  • Cell or microcarrier material 74 passes thorough the thick wall hollow fibers 60 and is released in the retentate flow 16.
  • the wall 70 includes tortuous paths 71 to capture certain elements of the flow 12 through the thick wall hollow fiber tangential flow filter 30 while allowing other particles to pass through into the permeate flow 24.
  • Settling zones 73 and narrowing channels 75 capture larger cell debris particles 72b which enter the tortuous paths 71 , while allowing smaller particles (for example proteins or viruses) 72a to pass through.
  • the settling zones 73 and the narrowing channels 75 of the tortuous paths 71 thus cause a separation of the depth strained particles 72b from the permeate flow 24 unlike filtering obtained by the skin or surface of common thin wall tangential flow filter membranes.
  • the thick wall hollow fibers 60 are preferably made from sintered Polyethylene (PE) which has a molecular structure of repeating -CH2 - CH2 units and may be coated with PVDF.
  • the hollow fibers preferably have an inside diameter (or lumen) between 1 .0mm and 7.0mm and more preferably about 3.15mm, a wall thickness preferably between 0.15cm and 0.40cm and more preferably between 0.265cm and 0.33cm, and the tortuous paths 71 preferably between ⁇ . ⁇ and 30 ⁇ pore size, for use in perfusion and harvest of mammalian cell culture or cultures with microcarriers either in Tangential Flow Filtration (TFF) or Alternating Tangential Flow (ATF) methodologies, providing the depth filtration of the material passing thru the wall 70.
  • the assembled hollow fiber tangential flow filter 30 is both gamma stable and autoclave able.
  • the present invention finds industrial applicability in the field of filtering biological material Scope of the Invention.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Manufacturing & Machinery (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Filtering Materials (AREA)
  • Filtration Of Liquid (AREA)
EP17724140.3A 2016-04-11 2017-04-11 Tangentialflussfilter aus dickwandigen hohlfasern Withdrawn EP3442689A1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15/095,435 US10711238B2 (en) 2012-10-02 2016-04-11 Method for proliferation of cells within a bioreactor using a disposable pumphead and filter assembly
PCT/US2017/026926 WO2017180573A1 (en) 2016-04-11 2017-04-11 Thick wall hollow fiber tangential flow filter

Publications (1)

Publication Number Publication Date
EP3442689A1 true EP3442689A1 (de) 2019-02-20

Family

ID=58710050

Family Applications (1)

Application Number Title Priority Date Filing Date
EP17724140.3A Withdrawn EP3442689A1 (de) 2016-04-11 2017-04-11 Tangentialflussfilter aus dickwandigen hohlfasern

Country Status (5)

Country Link
EP (1) EP3442689A1 (de)
JP (1) JP2019513555A (de)
KR (2) KR20180126077A (de)
CN (1) CN109414656A (de)
WO (1) WO2017180573A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10358626B2 (en) 2016-04-12 2019-07-23 Artemis Biosystems, Inc. Perfusion filtration systems
US10538727B2 (en) * 2018-03-08 2020-01-21 Repligen Corporation Tangential flow depth filtration systems and methods of filtration using same
US11028124B2 (en) 2018-05-07 2021-06-08 Repligen Corporation Methods, devices and systems for 3-stage filtration
KR20230079245A (ko) * 2018-05-25 2023-06-05 리플리겐 코포레이션 접선 유동 여과 시스템 및 방법
EP3906107A4 (de) * 2018-12-31 2023-02-22 Repligen Corporation Filter für säugerzellkulturperfusion und klärung mit hydrophober hohlfaser
WO2020163454A1 (en) 2019-02-08 2020-08-13 Lonza Walkersville, Inc. Cell concentration methods and devices for use in automated bioreactors
AU2020343312B2 (en) * 2019-09-06 2023-07-13 Repligen Corporation Scale-down tangential flow depth filtration systems and methods of filtration using same
WO2023141196A1 (en) 2022-01-21 2023-07-27 Repligen Corporation Systems and methods for filtration of cell cultures

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102008899B (zh) * 2010-11-30 2012-02-22 东莞市威迪膜科技有限公司 一种固液分离管式微滤膜的制备方法

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FR2600264B1 (fr) * 1986-06-20 1990-06-08 Commissariat Energie Atomique Element d'ultrafiltration, d'hyperfiltration ou de demineralisation son procede de fabrication et son utilisation pour le traitement d'effluents liquides radioactifs
DE3900020A1 (de) * 1989-01-02 1990-07-05 Akzo Gmbh Verfahren zur stoffuebertragung
FR2668077B1 (fr) * 1990-10-22 1992-12-04 Commissariat Energie Atomique Membrane d'osmose inverse ou de nanofiltration et son procede de fabrication.
US5721031A (en) * 1993-07-21 1998-02-24 Unitika Ltd. Fiber-reinforced porous plastic tube
JP3668283B2 (ja) * 1995-05-16 2005-07-06 三菱樹脂株式会社 多孔質複層プラスチックフィルタ及びその製造方法
JP3681575B2 (ja) * 1999-05-25 2005-08-10 三菱樹脂株式会社 多孔質プラスチックフィルタ
JP2002066280A (ja) * 2000-08-30 2002-03-05 Kyocera Corp ガス分離フィルタおよびその製造方法
WO2005047857A2 (en) * 2003-11-04 2005-05-26 Porex Corporation Composite porous materials and methods of making and using the same
KR101257959B1 (ko) * 2003-12-22 2013-04-24 엔테그리스, 아이엔씨. 포팅된 중공 도관을 구비한 교환 장치
NZ579779A (en) * 2007-04-04 2012-10-26 Siemens Industry Inc Filtration system including fine apertured screen
CN201263972Y (zh) * 2008-08-06 2009-07-01 孟广祯 曝气内压膜过滤器
US20100108599A1 (en) * 2008-11-03 2010-05-06 Kristof Vizvardi Filtration membrane with tubular support
JP2013150961A (ja) * 2012-01-26 2013-08-08 Sekisui Chem Co Ltd 高分子水処理膜の製造方法
CN102698611A (zh) * 2012-06-27 2012-10-03 上海大学 湿法纺丝法制备聚砜酰胺中空纤维分离膜的方法
DE102013010724A1 (de) * 2013-06-27 2014-12-31 Mann+Hummel Gmbh Polymeres Vollbluthohlfasermembranfiltermedium und Verwendung davon zum Abtrennen von Blutplasma/-serum von Vollblut
CN103752187B (zh) * 2014-01-09 2016-04-13 天津工业大学 一种具有不对称孔结构玻璃中空纤维纳滤膜的制备方法
DE102014108230A1 (de) * 2014-06-12 2015-12-17 B. Braun Avitum Ag Dialysator mit einem Bündel an Hohlfasern sowie Verfahren zum Herstellen einer solchen Hohlfaser

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102008899B (zh) * 2010-11-30 2012-02-22 东莞市威迪膜科技有限公司 一种固液分离管式微滤膜的制备方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BROADHEAD K W ET AL: "Effects of fabrication conditions on the structure and function of membranes formed from poly(acrylonitrile-vinylchloride)", JOURNAL OF MEMBRANE SCIENCE, ELSEVIER BV, NL, vol. 147, no. 2, 2 September 1998 (1998-09-02), pages 235 - 245, XP004147086, ISSN: 0376-7388, DOI: 10.1016/S0376-7388(98)00125-2 *
See also references of WO2017180573A1 *

Also Published As

Publication number Publication date
CN109414656A (zh) 2019-03-01
JP2019513555A (ja) 2019-05-30
KR20180126077A (ko) 2018-11-26
WO2017180573A1 (en) 2017-10-19
KR20220019059A (ko) 2022-02-15

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