EP3442689A1 - Thick wall hollow fiber tangential flow filter - Google Patents

Thick wall hollow fiber tangential flow filter

Info

Publication number
EP3442689A1
EP3442689A1 EP17724140.3A EP17724140A EP3442689A1 EP 3442689 A1 EP3442689 A1 EP 3442689A1 EP 17724140 A EP17724140 A EP 17724140A EP 3442689 A1 EP3442689 A1 EP 3442689A1
Authority
EP
European Patent Office
Prior art keywords
thick wall
wall hollow
twhftff
hollow fiber
hollow fibers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP17724140.3A
Other languages
German (de)
French (fr)
Inventor
David Serway
Michael BRANSBY
Steve LE
Bao Le
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Spectrum Inc
Original Assignee
Spectrum Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US15/095,435 external-priority patent/US10711238B2/en
Application filed by Spectrum Inc filed Critical Spectrum Inc
Publication of EP3442689A1 publication Critical patent/EP3442689A1/en
Pending legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/08—Hollow fibre membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
    • B01D29/88—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor having feed or discharge devices
    • B01D29/90—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor having feed or discharge devices for feeding
    • B01D29/908—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor having feed or discharge devices for feeding provoking a tangential stream
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D39/00—Filtering material for liquid or gaseous fluids
    • B01D39/14—Other self-supporting filtering material ; Other filtering material
    • B01D39/16—Other self-supporting filtering material ; Other filtering material of organic material, e.g. synthetic fibres
    • B01D39/1692—Other shaped material, e.g. perforated or porous sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
    • B01D67/0081—After-treatment of organic or inorganic membranes
    • B01D67/0088—Physical treatment with compounds, e.g. swelling, coating or impregnation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/04—Tubular membranes
    • B01D69/043—Tubular membranes characterised by the tube diameter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/26—Polyalkenes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/26—Polyalkenes
    • B01D71/261—Polyethylene
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2325/00—Details relating to properties of membranes
    • B01D2325/04—Characteristic thickness
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/30—Polyalkenyl halides
    • B01D71/32—Polyalkenyl halides containing fluorine atoms
    • B01D71/34—Polyvinylidene fluoride

Definitions

  • the present invention relates to filters and in particular to a thick wall tangential flow filter.
  • the present invention addresses the above and other needs by providing a Thick Wall Hollow Fiber Tangential Flow Filter (TWHFTFF) for perfusion and harvest of mammalian cell culture or cultures.
  • TWHFTFF Thick Wall Hollow Fiber Tangential Flow Filter
  • the TWHFTFF includes at least one thick wall hollow fiber reaching from an inlet to an outlet.
  • the thick wall hollow fiber including thick walls having tortuous paths between an interior surface and an exterior surface of the thick wall hollow fiber.
  • the tortuous paths include settling zones which capture small particles and narrowed zones which capture larger particles.
  • the walls may be made of HDPE or plastic materials including PES, PVDF and may be coated with PVDF.
  • a tangential flow filter including thick wall hollow fibers.
  • the thick wall hollow fibers include tortuous paths which selectively capture particles stripped from a retentate flow through the filter and a permeate from the filter.
  • FIG. 1 shows a cross-sectional view of a hollow fiber tangential flow filter according to the present invention.
  • FIG. 2 shows a cross-sectional view of a wall of a thick wall hollow fiber tangential flow filter according to the present invention.
  • FIG. 1 A cross-sectional view of the hollow fiber tangential flow filter 30 is shown in FIG. 1 .
  • the hollow fiber tangential flow filter 30 includes parallel thick wall hollow fibers 60 residing between an inlet chamber 30a and an outlet cham ber 30b.
  • An inlet port 32a provides a flow 12 to the inlet chamber 30a and an outlet port 32d receives a retentate flow 16 from the outlet chamber 30b.
  • the thick wall hollow fibers 60 receive the flow 12 through the inlet chamber 30a.
  • the flow 12 advanced through a hollow fiber interior 60a of the thick wall hollow fibers 60 and releases a permeate flow (for example, protein or waste material) 24 through thick walls 70 into a permeate section 61 of the filter housing 31 .
  • a permeate flow for example, protein or waste material
  • the permeate flow 24 travels to permeate ports 32b and 32c and is released or may be drawn from the filter housing 31 by a pump.
  • the filtered retentate flow 16 is released from the thick wall hollow fibers 60 into the outlet chamber 30b and is released from the filter 30 through retentate port 32d.
  • FIG. 2 A cross-sectional view of a thick wall 70 of the thick wall hollow fibers 60 of the thick wall hollow fiber tangential flow filter 30 is shown in FIG. 2.
  • Cell or microcarrier material 74 passes thorough the thick wall hollow fibers 60 and is released in the retentate flow 16.
  • the wall 70 includes tortuous paths 71 to capture certain elements of the flow 12 through the thick wall hollow fiber tangential flow filter 30 while allowing other particles to pass through into the permeate flow 24.
  • Settling zones 73 and narrowing channels 75 capture larger cell debris particles 72b which enter the tortuous paths 71 , while allowing smaller particles (for example proteins or viruses) 72a to pass through.
  • the settling zones 73 and the narrowing channels 75 of the tortuous paths 71 thus cause a separation of the depth strained particles 72b from the permeate flow 24 unlike filtering obtained by the skin or surface of common thin wall tangential flow filter membranes.
  • the thick wall hollow fibers 60 are preferably made from sintered Polyethylene (PE) which has a molecular structure of repeating -CH2 - CH2 units and may be coated with PVDF.
  • the hollow fibers preferably have an inside diameter (or lumen) between 1 .0mm and 7.0mm and more preferably about 3.15mm, a wall thickness preferably between 0.15cm and 0.40cm and more preferably between 0.265cm and 0.33cm, and the tortuous paths 71 preferably between ⁇ . ⁇ and 30 ⁇ pore size, for use in perfusion and harvest of mammalian cell culture or cultures with microcarriers either in Tangential Flow Filtration (TFF) or Alternating Tangential Flow (ATF) methodologies, providing the depth filtration of the material passing thru the wall 70.
  • the assembled hollow fiber tangential flow filter 30 is both gamma stable and autoclave able.
  • the present invention finds industrial applicability in the field of filtering biological material Scope of the Invention.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Manufacturing & Machinery (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Filtering Materials (AREA)
  • Filtration Of Liquid (AREA)

Abstract

A Thick Wall Hollow Fiber Tangential Flow Filter (TWHFTFF) (10) for perfusion and harvest of mammalian cell culture or cultures (74). The TWHFTFF (10) includes at least one thick wall hollow fiber (60) reaching from an inlet (32a) to an outlet (32b). The thick wall hollow fiber (60) including thick walls (70) having tortuous paths (71 ) between an interior surface and an exterior surface of the thick wall hollow fiber (60). The tortuous paths (71 ) include settling zones (73) and narrowed zones (75) which capture larger particles (72b). The walls may (70) be made of HDPE or plastic materials including PES, PVDF and may be coated with PVDF.

Description

S P E C I F I C A T I O N
THICK WALL HOLLOW FIBER TANGENTIAL FLOW FILTER
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] The present application is a Continuation In Part of US Patent Application Serial No. 15/095,435 filed April 1 1 , 2016, which application incorporated in its entirety herein by reference.
Technical field
[0002] The present invention relates to filters and in particular to a thick wall tangential flow filter.
Background Art
[0003] Known skin or surface of common thin wall tangential flow filter membranes fail to capture some elements of a flow through the filter. There is a need for a tangential flow filter capable of capturing specific elements of a permeate flow
Disclosure of the Invention
[0004] The present invention addresses the above and other needs by providing a Thick Wall Hollow Fiber Tangential Flow Filter (TWHFTFF) for perfusion and harvest of mammalian cell culture or cultures. The TWHFTFF includes at least one thick wall hollow fiber reaching from an inlet to an outlet. The thick wall hollow fiber including thick walls having tortuous paths between an interior surface and an exterior surface of the thick wall hollow fiber. The tortuous paths include settling zones which capture small particles and narrowed zones which capture larger particles. The walls may be made of HDPE or plastic materials including PES, PVDF and may be coated with PVDF.
[0005] In accordance with one aspect of the invention, there is provided a tangential flow filter including thick wall hollow fibers. The thick wall hollow fibers include tortuous paths which selectively capture particles stripped from a retentate flow through the filter and a permeate from the filter.
Brief Description of the Drawing
[0006] The above and other aspects, features and advantages of the present invention will be more apparent from the following more particular description thereof, presented in conjunction with the following drawings wherein:
[0007] FIG. 1 shows a cross-sectional view of a hollow fiber tangential flow filter according to the present invention.
[0008] FIG. 2 shows a cross-sectional view of a wall of a thick wall hollow fiber tangential flow filter according to the present invention.
[0009] Corresponding reference characters indicate corresponding components throughout the several views of the drawings.
Best Mode for Carrying out the Invention
[0010] The following description is of the best mode presently contemplated for carrying out the invention. This description is not to be taken in a lim iting sense, but is made merely for the purpose of describing one or more preferred embodiments of the invention. The scope of the invention should be determined with reference to the claims.
[0011] Where the term "generally" is associated with an element of the invention, it is intended to describe a feature's appearance to the human perception, and not a precise measurement.
[0012] A cross-sectional view of the hollow fiber tangential flow filter 30 is shown in FIG. 1 . The hollow fiber tangential flow filter 30 includes parallel thick wall hollow fibers 60 residing between an inlet chamber 30a and an outlet cham ber 30b. An inlet port 32a provides a flow 12 to the inlet chamber 30a and an outlet port 32d receives a retentate flow 16 from the outlet chamber 30b. The thick wall hollow fibers 60 receive the flow 12 through the inlet chamber 30a. The flow 12 advanced through a hollow fiber interior 60a of the thick wall hollow fibers 60 and releases a permeate flow (for example, protein or waste material) 24 through thick walls 70 into a permeate section 61 of the filter housing 31 . The permeate flow 24 travels to permeate ports 32b and 32c and is released or may be drawn from the filter housing 31 by a pump. The filtered retentate flow 16 is released from the thick wall hollow fibers 60 into the outlet chamber 30b and is released from the filter 30 through retentate port 32d.
[0013] A cross-sectional view of a thick wall 70 of the thick wall hollow fibers 60 of the thick wall hollow fiber tangential flow filter 30 is shown in FIG. 2. Cell or microcarrier material 74 passes thorough the thick wall hollow fibers 60 and is released in the retentate flow 16. [0014] The wall 70 includes tortuous paths 71 to capture certain elements of the flow 12 through the thick wall hollow fiber tangential flow filter 30 while allowing other particles to pass through into the permeate flow 24. Settling zones 73 and narrowing channels 75 capture larger cell debris particles 72b which enter the tortuous paths 71 , while allowing smaller particles (for example proteins or viruses) 72a to pass through. The settling zones 73 and the narrowing channels 75 of the tortuous paths 71 thus cause a separation of the depth strained particles 72b from the permeate flow 24 unlike filtering obtained by the skin or surface of common thin wall tangential flow filter membranes.
[0015] In one embodiment the thick wall hollow fibers 60 are preferably made from sintered Polyethylene (PE) which has a molecular structure of repeating -CH2 - CH2 units and may be coated with PVDF. The hollow fibers preferably have an inside diameter (or lumen) between 1 .0mm and 7.0mm and more preferably about 3.15mm, a wall thickness preferably between 0.15cm and 0.40cm and more preferably between 0.265cm and 0.33cm, and the tortuous paths 71 preferably between Ο.δμιη and 30μΐΎΐ pore size, for use in perfusion and harvest of mammalian cell culture or cultures with microcarriers either in Tangential Flow Filtration (TFF) or Alternating Tangential Flow (ATF) methodologies, providing the depth filtration of the material passing thru the wall 70. The assembled hollow fiber tangential flow filter 30 is both gamma stable and autoclave able.
[0016] Industrial Applicability
[0017] The present invention finds industrial applicability in the field of filtering biological material Scope of the Invention.
[0018] While the invention herein disclosed has been described by means of specific embodiments and applications thereof, numerous modifications and variations could be made thereto by those skilled in the art without departing from the scope of the invention set forth in the claims.

Claims

I CLAIM:
^ ^ A Thick Wall Hollow Fiber Tangential Flow Filter (TWHFTFF), comprising:
2 a housing;
3 a housing interior;
4 an inlet into the housing interior;
5 an inlet chamber in fluid communication with the inlet;
6 a retentate outlet from the housing interior;
7 an outlet chamber in fluid communication with the retentate outlet;
8 at least one permeate outlet from the housing interior
9 at least one thick wall hollow fiber residing in the housing interior and
0 extending between the inlet chamber and the outlet chamber, the inlet chamber and 1 outlet chamber in fluid communication with an interior of the thick wall hollow fiber; 2 walls of the thick wall hollow fibers, the walls comprising:
3 an interior surface;
4 an exterior surface;
5 a thickness between 0.265cm and 0.33cm ;
6 tortuous paths between the interior surface and the exterior surface,7 the tortuous paths including:
8 settling zones to capture small particles; and
9 narrowing channels to capture depth strained particles..
2. The TWHFTFF of Claim 1 , wherein the tortuous paths have between Ο.δμιη and 30μΐΎΐ pore size.
3. The TWHFTFF of Claim 1 , wherein the thick wall hollow fibers have an inside diameter between 1 mm and 7mm.
4. The TWHFTFF of Claim 3, wherein the thick wall hollow fibers have an inside diameter of about 3.15mm
5. The TWHFTFF of Claim 1 , wherein the thick wall hollow fibers have a wall thickness between 0.15cm and 0.40cm.
6. The TWHFTFF of Claim 5, wherein the thick wall hollow fibers have a wall thickness between 0.265cm and 0.33cm.
7. The TWHFTFF of Claim 1 , wherein the thick wall hollow fibers are made from sintered material.
8. The TWHFTFF of Claim 7, wherein the sintered material has a molecular structure of repeating -CH2 - CH2 units
9. The TWHFTFF of Claim 7, wherein the sintered material is coated with PVDF
EP17724140.3A 2016-04-11 2017-04-11 Thick wall hollow fiber tangential flow filter Pending EP3442689A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15/095,435 US10711238B2 (en) 2012-10-02 2016-04-11 Method for proliferation of cells within a bioreactor using a disposable pumphead and filter assembly
PCT/US2017/026926 WO2017180573A1 (en) 2016-04-11 2017-04-11 Thick wall hollow fiber tangential flow filter

Publications (1)

Publication Number Publication Date
EP3442689A1 true EP3442689A1 (en) 2019-02-20

Family

ID=58710050

Family Applications (1)

Application Number Title Priority Date Filing Date
EP17724140.3A Pending EP3442689A1 (en) 2016-04-11 2017-04-11 Thick wall hollow fiber tangential flow filter

Country Status (5)

Country Link
EP (1) EP3442689A1 (en)
JP (1) JP2019513555A (en)
KR (2) KR20180126077A (en)
CN (1) CN109414656A (en)
WO (1) WO2017180573A1 (en)

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CN111902197B (en) * 2018-03-08 2022-10-28 瑞普利金公司 Tangential flow depth filtration system and method for filtration using same
US11028124B2 (en) * 2018-05-07 2021-06-08 Repligen Corporation Methods, devices and systems for 3-stage filtration
JP2021523830A (en) * 2018-05-25 2021-09-09 レプリゲン・コーポレイションＲｅｐｌｉｇｅｎ Ｃｏｒｐｏｒａｔｉｏｎ Tangential flow filtration system and method
CN113226510A (en) * 2018-12-31 2021-08-06 瑞普利金公司 Hydrophobic hollow fiber filter for perfusion and clarification of mammalian cell culture
EP4025323A4 (en) * 2019-09-06 2023-03-01 Repligen Corporation Scale-down tangential flow depth filtration systems and methods of filtration using same
US20230235263A1 (en) 2022-01-21 2023-07-27 Repligen Corporation Systems and methods for filtration of cell cultures

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Also Published As

Publication number Publication date
KR20220019059A (en) 2022-02-15
CN109414656A (en) 2019-03-01
JP2019513555A (en) 2019-05-30
WO2017180573A1 (en) 2017-10-19
KR20180126077A (en) 2018-11-26

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