TWM557639U - Thick wall hollow fiber tangential flow filter - Google Patents

Abstract

A Thick Wall Hollow Fiber Tangential Flow Filter (TWHFTFF) for perfusion and harvest of mammalian cell culture or cultures. The TWHFTFF includes at least one thick wall hollow fiber reaching from an inlet to an outlet. The thick wall hollow fiber including thick walls having tortuous paths between an interior surface and an exterior surface of the thick wall hollow fiber. The tortuous paths include settling zones and narrowed zones which capture larger particles. The walls may be made of HDPE or plastic materials including PES, PVDF and may be coated with PVDF.

Description

Tangential fluid filter material composed of thick-walled hollow fiber

This creation is about a filter material, especially a thick-walled tangential fluid filter.

Common thin-walled tangential fluid filter materials do not capture the elements of the fluid flow filter in the surface or surface. Therefore, a tangential fluid screening filter is required to capture specific elements in the fluid and then seep out the filter material.

In view of the foregoing problems, the present inventors have further developed a tangential fluid filter material composed of a thick-walled hollow fiber for perfusing and capturing mammalian cell culture, which mainly comprises a filter body including a filter body inside. The filter body has an input port for the fluid input filter body, an input area for communicating with the input port, an output port for discharging the retained fluid from the filter body, an output area for communicating with the output port, and at least one or more a bleed end outputted from the inside of the filter body, one or more thick-walled hollow fibers between the input zone and the output zone inside the filter body, wherein the thick-walled hollow fiber is provided with a hollow fiber inside between the input zone and the output zone, The thick-walled hollow fiber comprises an inner layer surface and an outer layer surface, and a tortuous path is provided between the inner layer surface and the outer layer surface, and the fixed area in the tortuous path is for capturing small molecules. The narrow channel is used to capture macromolecules. The thick wall can be coated with polyvinylidene fluoride from high molecular weight polyethylene or other plastic materials such as polyether or polyvinylidene fluoride.

The present invention provides a tangential fluid filter material composed of thick-walled hollow fibers, which allows the retained fluid to ooze out of the filter material by flowing through the thick-walled hollow fiber filter material to capture specific elements.

12‧‧‧ fluid

16‧‧‧Retained fluid

24‧‧‧Exuding fluid

30‧‧‧Fluid filter

30a‧‧‧Input area

30b‧‧‧Output area

31‧‧‧ Filter

32a‧‧ Input port

32b, 32c‧‧‧ oozing end

32d‧‧‧ outlet

60‧‧‧ Thick-walled hollow fiber

60a‧‧‧ hollow fiber interior

61‧‧‧Infiltration zone

70‧‧‧ thick wall

71‧‧‧ Tortuous path

72a‧‧‧Small particles

72b‧‧‧ Large particles

73‧‧‧Setting area

74‧‧‧Microcarriers

75‧‧‧Narrow channel

The first picture is a cross-sectional view of the creation.

The second figure is a partial enlarged cross-sectional view of the present creation.

The following description is the best mode currently contemplated for implementing this creation. The description is not to be considered as limiting, but only for the purpose of describing one or more preferred embodiments of the present invention. The scope of the present invention should be determined by reference to the scope of the patent.

Often the relevance of the various elements in the present teachings is intended to be through the technical features that are generally known, rather than the precise description.

The cross-sectional view shown in the first figure is a tangential fluid filter 30 composed of hollow fibers of the present invention. The tangential fluid filter 30 includes thick-walled hollow fibers 60 arranged in parallel between the input zone 30a and the output zone 30b. The port 32a receives fluid 12 into the input zone 30a and is received by the outlet 32d to filter the retentate fluid 16 produced in the output zone 30b. The thick-walled hollow fiber 60 receives the fluid 12 via the input zone 30a, and the fluid 12 passes through the thick-walled hollow fiber 6 first. After the hollow fiber interior 60a of 0, fluid 24 (e.g., protein or waste) is oozing out through the permeate zone 61 of the thick wall 70 in the filter body 31, and the fluid 24 flows to the bleed end 32b or 32c or by the pump from the filter body 31. The discharged, retentate fluid 16 is released from the thick-walled hollow fiber 60 into the output zone 30b, and is then output from the output port 32d of the fluid filter material 30.

The partially enlarged cross-sectional view shown in the second figure is the thick wall 70 of the thick-walled hollow fiber 60 of the tangential fluid filter material 30 composed of the hollow fiber of the present invention. After the cell or microcarrier 74 flows through the thick-walled hollow fiber 60, it will be filtered and retained. Fluid 16.

The fluid 12 flows through the tortuous path 71 of the thick wall 70 in the fluid filter material 30 to permeate the exudate fluid 24, and the fixed area 73 and the narrow channel 75 in the tortuous path 71 capture the cell debris 72b, allowing the small molecule 72a (for example The protein or virus passes, so that the fixed region 73 and the narrow channel 75 in the tortuous channel 71 can separate the macromolecule 72b from the effluent fluid 24, which is an element of the thin-walled tangential fluid filter material whose surface layer or surface cannot be captured.

In a preferred embodiment, the thick-walled hollow fiber 60 is made of high-molecular polyethylene (PE) and coated on the surface thereof with polyvinylidene fluoride (PVDF). The inner diameter of the hollow fiber is preferably 1.0 to 7.0. Between PCT, the optimum is about 3.15 mm, the wall thickness is preferably between 0.15 and 0.40 cm, and the best is between 0.265 and 0.33 cm. The capillary channel 71 has a capillary diameter of 0.5 to 30 nm. In the tangential fluid filtration (TFF) or alternating tangential fluid (ATF) method, it is suitable for microcarrier perfusion and capture of mammalian cell cultures, providing a mechanism for allowing fluid to pass through and deep filtration. The thick wall 70, the fluid filter material 30 can withstand gamma stabilization treatment and retort treatment.

To be clear, the above is the specific embodiment of the creation and the technical principles applied. If the changes made according to the concept of this creation, the functional role produced by it does not exceed the spirit of the manual and the drawings. At the time, it should be combined with Chen Ming within the scope of this creation.

Claims (9)

A tangential fluid filter material composed of thick-walled hollow fibers, comprising: a filter body; a filter body; an input port for supplying fluid into the filter body; an input region connected to the input port; and a retaining fluid An output port outputted from the inside of the filter body; an output area communicating with the output port; at least one exudate end outputted from the inside of the filter body; at least one thick-walled hollow fiber between the input zone and the output zone inside the filter body The thick-walled hollow fiber is provided with a hollow fiber inside between the input zone and the output zone; the thick-walled hollow fiber comprises an inner layer surface and an outer layer surface, and the thick wall thickness is between 0.265 and 0.33 cm. There is a tortuous path between the surface of the layer and the surface of the outer layer. The fixed area in the tortuous channel is for capturing small molecules, and the narrow channel is for capturing large molecules. The tangential fluid filter material of the thick-walled hollow fiber according to claim 1, wherein the meandering channel has an inner diameter of between 0.5 and 30 nm. The tangential fluid filter material of the thick-walled hollow fiber according to claim 1, wherein the thick-walled hollow fiber has an inner diameter of between 1 and 7 cm. Such as the thick-walled hollow fiber described in the third paragraph of the patent application scope To the fluid filter material, wherein the thick-walled hollow fiber has an inner diameter of about 3.15 cm. The tangential fluid filter material of the thick-walled hollow fiber according to claim 1, wherein the thick-walled hollow fiber has a wall thickness of between 0.15 and 0.40 cm. A tangential fluid filter material comprising thick-walled hollow fibers according to claim 5, wherein the thick-walled hollow fiber has a wall thickness of between 0.265 and 0.33 cm. A tangential fluid filter material comprising thick-walled hollow fibers according to claim 1, wherein the thick-walled hollow fiber is made of a sintered material. A tangential fluid filter material comprising thick-walled hollow fibers according to claim 7, wherein the sintered material is polyethylene. A tangential fluid filter material comprising thick-walled hollow fibers according to claim 7, wherein the sintered material can be coated with polyvinylidene fluoride.