EP3417943B1 - Rotor de centrifugeuse doté de joint d'étanchéité - Google Patents

Rotor de centrifugeuse doté de joint d'étanchéité Download PDF

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Publication number
EP3417943B1
EP3417943B1 EP17177239.5A EP17177239A EP3417943B1 EP 3417943 B1 EP3417943 B1 EP 3417943B1 EP 17177239 A EP17177239 A EP 17177239A EP 3417943 B1 EP3417943 B1 EP 3417943B1
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EP
European Patent Office
Prior art keywords
centrifuge rotor
cover
groove
centrifuge
elements
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP17177239.5A
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German (de)
English (en)
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EP3417943A1 (fr
Inventor
Steffen Kühnert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
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Eppendorf SE
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Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Priority to EP17177239.5A priority Critical patent/EP3417943B1/fr
Priority to CN201880049122.6A priority patent/CN110997153B/zh
Priority to US16/625,634 priority patent/US11471897B2/en
Priority to JP2019570892A priority patent/JP6967616B2/ja
Priority to PCT/EP2018/066307 priority patent/WO2018234334A1/fr
Publication of EP3417943A1 publication Critical patent/EP3417943A1/fr
Application granted granted Critical
Publication of EP3417943B1 publication Critical patent/EP3417943B1/fr
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B5/00Other centrifuges
    • B04B5/04Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B7/00Elements of centrifuges
    • B04B7/08Rotary bowls
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B7/00Elements of centrifuges
    • B04B7/02Casings; Lids
    • B04B2007/025Lids for laboratory centrifuge rotors

Definitions

  • the present invention relates to a centrifuge rotor according to the preamble of claim 1.
  • a centrifuge rotor according to the preamble of claim 1 is from GB 2 233 584 A1 known.
  • Centrifuge rotors are used in centrifuges, in particular laboratory centrifuges, in order to separate the components from samples centrifuged therein, using the inertia. In order to achieve high segregation rates, ever higher rotation speeds are used.
  • Laboratory centrifuges are centrifuges whose rotors operate at preferably at least 3,000, preferably at least 10,000, in particular at least 15,000 revolutions per minute and are usually placed on tables. In order to be able to place them on a work table, they have in particular a form factor of less than 1 m x 1 m x 1 m, so their installation space is limited.
  • the device depth is max. Limited to 70 cm.
  • samples are centrifuged at certain temperatures. For example, samples containing proteins and the like. Organic substances must not be overheated, so that the upper limit for the temperature control of such samples is in the range of + 40 ° C as standard. On the other hand, certain samples are cooled by default in the range of + 4 ° C (the anomaly of the water starts at + 3.98 ° C).
  • Active and passive systems can be used for temperature control.
  • Active cooling systems have a refrigerant circuit that tempers the centrifuge bowl, which indirectly cools the centrifuge rotor and the sample containers it contains.
  • Passive systems are based on exhaust air-assisted cooling or ventilation. This air is led directly past the centrifuge rotor, which means that the temperature is controlled. The air is sucked through openings in the centrifuge bowl, the suction taking place automatically by the rotation of the centrifuge rotor.
  • the samples to be centrifuged are stored in sample containers and these sample containers are driven in rotation by means of a centrifuge rotor.
  • a centrifuge rotor There are various centrifuge rotors that are used depending on the application.
  • the sample containers can contain the samples directly, or separate sample containers containing the sample are used in the sample containers, so that a large number of samples can be centrifuged simultaneously in one sample container.
  • centrifuge rotors have a lower part and a lid, with an inner space being formed between the lower part and the lid when the lid is closed, in which the sample vessels can be arranged in order to centrifuge the samples in a suitable centrifuge. If the sample vessels are arranged in the centrifuge rotor at a predetermined angle, then it is a so-called fixed-angle rotor.
  • the lower part For connection to the centrifuge, the lower part is usually provided with a hub which can be coupled to the motor-driven drive shaft of the centrifuge.
  • the cover in turn is usually screwed to the lower part.
  • a fluid-tight seal usually takes place between the lid and the lower part, for example that of the fixed-angle rotor FA-45-48-11 from Eppendorf®, which can be used, for example, in the laboratory centrifuge 5430 R from Eppendorf®, has a disc-like lid, in which is arranged a radially open groove, the groove containing an O-ring as a sealant.
  • the cover When closing, the cover is inserted into a corresponding approximately vertical recess of the lower part and clamped downwards, the O-ring being clamped between the groove and the side wall of the lower part, in order to thereby effect the seal.
  • the seal should be more effective and long-lasting.
  • the opening and closing process should preferably be facilitated.
  • this object can be achieved in a surprisingly simple manner in that the groove for holding the sealing means is arranged in such a way that it is axially aligned, namely axially from one of the two cover elements and the lower part to the other of the two cover elements and lower part open. Then the sealant cannot twist or open as much when opening and closing. In addition, centrifugation prevents the sealant from being ejected from the groove.
  • the centrifuge rotor according to the invention thus has a lower part and a lid, wherein sample vessels can be arranged in the centrifuge rotor, which are secured against removal in the closed state of the centrifuge rotor, an interior being formed in the closed state of the centrifuge rotor between the lower part and the lid, between the lower part and cover is a seal that the Seals the interior in a fluid-tight manner from the surroundings of the centrifuge rotor, the seal having a sealant which is arranged in a first groove, the first groove being arranged on one of the elements of the cover and lower part, the first groove with respect to the axis of rotation (R) of the centrifuge rotor is designed to be open axially towards the other of the cover and lower part, the other of the cover and lower part having a first section which extends axially towards one of the cover and lower part and which, when closed, extends into the first groove) , and is characterized in that the sealing means has a radially extending base and
  • the sealing means has a radially extending base and an axially extending leg arranged thereon.
  • the axial leg provides a particularly effective seal, for which only very low contact pressures are sufficient.
  • the leg becomes thicker towards the base and is preferably conical at least on one side, the conicity preferably being in the range 2 ° -10 °, preferably 4 ° -8 ° and in particular 6 °. This means that the seal is particularly even, even with tolerances.
  • the other of the elements cover and lower part in the closed state is at least on the leg. This makes sealing particularly effective.
  • the other of the cover and lower part has a first section which extends axially towards one of the cover and lower part and which, when closed, extends into the first groove. This enables very high contact pressures to be achieved and held securely. In addition, the groove overlaps the first section, making the seal very secure and protected.
  • the lower part has a section below the sealing means, which extends radially outward, in particular inclined, in the direction away from the cover. As a result, any fluids that may occur are directed away from the seal.
  • This inclined section preferably adjoins the first section when it is arranged on the lower part.
  • fluids mean both gases and liquids.
  • the lower part has a groove below the sealing means, which is preferably located radially further out than the sealing means.
  • the other of the cover and lower part has a second groove which opens axially towards one of the cover and lower part and which interacts with the first groove in the closed state. This creates a particularly secure seal. In addition, the seal is centered and the placement of the lid on the lower part is facilitated.
  • the first section delimits the second groove radially inwards. Then there is a particularly secure seal because there is a meandering engagement between the two grooves, any fluid that may occur in the operating state being rejected by the seal.
  • the first groove has a radially inner first and a radially outer second boundary, which are preferably designed as projections.
  • the lid is particularly lightweight, which simplifies centrifugation.
  • the first boundary extends axially lower in the direction of the lower part than the second boundary. Then the seal is particularly effective and protected.
  • the first section of the lower part is covered by the first boundary in the closed state. Then the seal is particularly effective and protected.
  • the centrifuge rotor is a bowl-shaped centrifuge rotor, which is designed in particular as a fixed-angle rotor.
  • the first groove is preferably formed on the cover and is open axially towards the lower part. Then the first section is arranged on the lower part and preferably delimits a second groove which interacts with the first groove.
  • first groove is formed on the lower part and is open axially towards the cover.
  • the first section is then arranged on the cover and preferably delimits a second groove which interacts with the first groove.
  • the centrifuge rotor 10 has a lower part 12 and a cover 14.
  • the centrifuge rotor 10 basically consists of a metal, preferably an aluminum-containing metal.
  • the lower part 12 there are bores 16 for receiving sample vessels (not shown).
  • the lower part 12 has a shaft holder 18 for receiving one Drive shaft of a suitable laboratory centrifuge 100 (for example the laboratory centrifuge 5430 R from Eppendorf®, not shown) on (cf. Fig. 4 ).
  • the lower part 12 has first closure means 20 known to the person skilled in the art, for example from the fixed-angle rotor FA-45-48-11 from Eppendorf®, which also comprise a rotor nut 22 with which the centrifuge rotor 10 is fastened to the drive shaft.
  • the cover 14 in turn has, for example, second closure means 24 known to the person skilled in the art from the fixed-angle rotor FA-45-48-11 from the Eppendorf® company with an actuating element 26 with which a user (not shown) places the cover 14 on the lower part 12 and can lock the second 24 with the first closure means 20.
  • the rotor nut 22 can also be rotated on the lower part 12 with the aid of the actuating element in the closed state of the cover 14, as a result of which the centrifuge rotor 10 can also be attached or detached to the drive shaft in the closed state and thus inserted into the centrifuge or removed from the centrifuge ,
  • the second closure element 24 with the actuating element 26 is connected in a sealed manner to the actual cover body 28, so that at this point no fluid can escape from an interior 30 which is formed between the cover 14 and the lower part 12 when the centrifuge rotor is closed.
  • a groove 32 is arranged in the lower part 12, namely below and in relation to an axis of rotation R of the centrifuge rotor 10 radially further outward than the seal 34 between the lower part 12 and the cover 14 such fluid is always diverted away from the seal 34 into the channel 32.
  • Fig. 2 is the seal 34 in an enlarged detail view of the area Z from Fig. 1 shown.
  • the cover 14 has a radially extending wall region 40, from which a first 42 and a second projection 44 extend axially downward towards the lower part 12.
  • the two projections 42, 44 are the lateral boundaries 42, 44 of a first groove 46 that opens between them axially downward toward the lower part 12.
  • the lower part 12 has a vertical, i.e. has axially extending wall area 48, from which a hook-like projection 50 extends radially inward into the interior 30.
  • a second groove 54 is formed by the upper wall section 52 of the wall region 48 and the projection 50, which opens axially upwards towards the cover 14.
  • the length of the upper wall section 52 corresponds to the length of the second projection 44 and that the length of the first projection 42 is formed such that the hook-like projection 50 in the closed state of the cover 14 on the lower part 12 through the first projection 42 is covered.
  • the hook-like projection 50 is connected to the wall area 48 by means of a deflector 56 which runs inclined outwards and downwards. As a result, any resulting fluid is drained away from the seal 34 into the channel 32.
  • the transition from the axial wall region 40 to the first projection 42 could also be made inclined (not shown) in order to improve fluid drainage.
  • the sealing element 60 which consists of a rubber material, is pressed into the first groove 46. It is particularly related to Fig. 3 It can be seen that the sealing element 60 has a radially extending base 62 and an axially extending leg 64 arranged thereon. For easy pressing into the first groove 46, the sealing element 60 has two chamfers 66 on the base 62.
  • the thickness of the leg 64 tapers away from the base 62.
  • the base has a thickness such that the hook-like projection 50 bears against the base 62 before the second projection 44 bears against the second groove 54.
  • the taper of the leg 62 provides a taper of the sealing element 60, through which the hook-like projection 50 is pressed against the leg 62 of the sealing element 60 the more the lid 14 is pressed onto the lower part 12.
  • the taper is preferably in the range 2 ° -10 ° and is in particular 6 °.
  • the cover 14 can be placed very easily on the lower part 12.
  • the seal 34 is always and permanently fluid-tight, because the taper of the leg 62 ensures secure contact of the hook-like projection 50 on the leg 62 even with dimensional tolerances.
  • first groove 46 opens axially downward prevents the sealant 60 from escaping from the first groove 46 due to centrifugation. In addition, the centrifugation only increases the sealing effect between leg 62 and hook-like projection 50.
  • Fig. 4 the centrifuge 100 according to the invention is shown, which has the centrifuge rotor 10 according to the invention. It can be seen that the laboratory centrifuge 100 has a housing 102 with a closable cover 104 in the usual manner, with corresponding drive means in the form of an electric motor, control means and cooling means (not shown) being used in the interior.
  • FIG. 5 shows a second preferred embodiment of the centrifuge rotor 200 according to the invention, specifically only the detailed view of the seal 202 being shown here. All other elements are essentially the same as the first preferred embodiment of the centrifuge rotor 10 according to FIGS 1 to 4 educated.
  • the cover 204 with a somewhat larger radius is designed such that the cover 204 clasps the lower part 206, while in FIG Fig. 2 It can be seen that the lower part 12 clasps the lid 14 there.
  • the first section 208 is arranged on the cover 204 and this first section 208 engages in the first groove 210, which is arranged with the sealing means 212 on the lower part 206.
  • the first groove 210 is thus designed to open axially towards the cover 204.
  • the second groove 211 is formed on the cover 204 and the first section 208 delimits the second groove 211 radially inwardly, while the second groove 211 is delimited on the outside by the circumferential collar 213.
  • the seal 202 is also very secure in this embodiment, but the first preferred embodiment is according to FIGS 1 to 4 even more advantageous, since the variant after Fig. 5 fluid that may occur may lie on the sealing means 212 between the first section 208 and the inner boundary 214 of the first groove 210, so that after opening the cover 204 the first groove 210 should be cleaned with the sealing means 212, which is the case with the first preferred embodiment 10 would not be necessary since fluid occurring there cannot get into the second groove 54.
  • sealant 212 is identical to the sealant 60 in FIG Fig. 2 is formed, wherein it is simply rotated by 180 ° with respect to the centrifuge rotor 10.
  • FIG. 6 A third preferred embodiment of the centrifuge rotor 300 according to the invention is shown, wherein here likewise only the detailed view of the seal 302 is shown concretely. All other elements are essentially the same as the first preferred embodiment of the centrifuge rotor 10 according to FIGS 1 to 4 educated.
  • the centrifuge rotor after Fig. 6 only differs from the design in that Fig. 5 that no outer circumferential collar (213 in Fig. 5 ) is provided, instead the cover 304 is delimited by the first section 306, which in turn engages in the first groove 308 on the lower part 310 and acts against the sealing means 312.
  • seal 34, 202 between the lower part 12, 206 and the lid 14, 204 of the centrifuge rotor 10, 200 is substantially improved with the present invention.
  • the seal 34, 202 used according to the invention is more effective and long-lasting than seals used previously. It also makes opening and closing easier.

Claims (10)

  1. Rotor de centrifugeuse (10 ; 200 ; 300) comprenant une partie inférieure (12 ; 206 ; 310) et un couvercle (14 ; 204 ; 304), des récipients à échantillon pouvant être disposés dans le rotor de centrifugeuse (10 ; 200 ; 300), lesquels sont protégés contre un retrait dans l'état fermé du rotor de centrifugeuse (10 ; 200 ; 300), un espace intérieur (30) se formant entre la partie inférieure (12 ; 206 ; 310) et le couvercle (14 ; 204 ; 304) dans l'état fermé du rotor de centrifugeuse (10 ; 200 ; 300), un joint d'étanchéité (34 ; 202 ; 302) se trouvant entre la partie inférieure (12 ; 206 ; 310) et le couvercle (14 ; 204 ; 304), ledit joint d'étanchéité fermant l'espace intérieur (30) de manière étanche aux fluides par rapport à l'environnement du rotor de centrifugeuse (10 ; 200 ; 300), le joint d'étanchéité (34 ; 202 ; 302) comportant un moyen d'étanchéité (60 ; 212 ; 312), qui est disposé dans une première rainure (46 ; 210 ; 308), la première rainure (46 ; 210 ; 308) étant disposée sur l'un des éléments couvercle (14 ; 204 ; 304) et partie inférieure (12 ; 206 ; 310), la première rainure (46 ; 210 ; 308) étant réalisée ouverte vers l'autre des éléments couvercle (14 ; 204 ; 304) et partie inférieure (12 ; 206 ; 310) dans le sens axial par rapport à l'axe de rotation (R) du rotor de centrifugeuse (10 ; 200 ; 300), l'autre des éléments couvercle (14 ; 204 ; 304) et partie inférieure (12 ; 206 ; 310) comportant une première section (50 ; 208 ; 306) s'étendant dans le sens axial vers l'un des éléments couvercle (14 ; 204 ; 304) et partie inférieure (12 ; 206 ; 310), ladite première section s'étendant à l'intérieur de la première rainure (46 ; 210 ; 308) dans l'état fermé, caractérisé en ce que le moyen d'étanchéité (60 ; 212 ; 312) comporte une base (62) s'étendant dans le sens radial et un côté (64) s'étendant dans le sens axial disposé sur celle-ci, l'autre des éléments couvercle (14 ; 204 ; 304) et partie inférieure (12 ; 206 ; 310) étant en appui au moins contre le côté (64) dans l'état fermé.
  2. Rotor de centrifugeuse (10 ; 200 ; 300) selon la revendication 1, caractérisé en ce que le côté (64) devient plus épais en direction de la base (62) et est réalisé conique de préférence au moins d'un côté, la conicité étant de préférence comprise dans l'intervalle de 2° à 10°, de préférence 4° à 8° et étant en particulier de 6°.
  3. Rotor de centrifugeuse (10 ; 200 ; 300) selon l'une de revendications précédentes, caractérisé en ce que la partie inférieure (12 ; 206 ; 310) comporte, sous le moyen d'étanchéité (60 ; 212 ; 312) une section (56), qui s'étend radialement vers l'extérieur, en particulier de manière inclinée, dans la direction s'éloignant du couvercle (14 ; 204 ; 304).
  4. Rotor de centrifugeuse (10 ; 200 ; 300) selon l'une de revendications précédentes, caractérisé en ce que la partie inférieure (12 ; 206 ; 310) comporte, sous le moyen d'étanchéité (60 ; 212 ; 312), une gorge (32), qui est disposée de préférence, vue dans le sens radial, plus à l'extérieur que le moyen d'étanchéité (60).
  5. Rotor de centrifugeuse (10 ; 200) selon l'une des revendications précédentes, caractérisé en ce que l'autre des éléments couvercle (14 ; 204 ; 304) et partie inférieure (12 ; 206 ; 310) comporte une seconde rainure (54 ; 211), qui s'ouvre axialement vers un des éléments couvercle (14) et partie inférieure (12), et qui interagit avec la première rainure (46 ; 210) dans l'état fermé.
  6. Rotor de centrifugeuse (10 ; 200) selon la revendication 5, caractérisé en ce que la première section (50 ; 208) limite la seconde rainure (54 ; 211) radialement vers l'intérieur.
  7. Rotor de centrifugeuse (10 ; 200 ; 300) selon l'une des revendications précédentes, caractérisé en ce que la première rainure (46 ; 210 ; 308) comporte une première limite (42 ; 208) située radialement à l'intérieur et une seconde limite (44) située radialement à l'extérieur, qui sont réalisées de préférence sous la forme de saillies.
  8. Rotor de centrifugeuse (10) selon la revendication 7, caractérisé en ce que la première limite (42) s'étend axialement plus profondément dans la direction de la partie inférieure (12) que la seconde limite (44).
  9. Rotor de centrifugeuse (10) selon la revendication 7 ou 8, caractérisé en ce que la première section (50) de la partie inférieure (12) est recouverte par la première limite (42) dans l'état fermé.
  10. Rotor de centrifugeuse (10 ; 200 ; 300) selon l'une des revendications précédentes, caractérisé en ce que le rotor de centrifugeuse est un rotor de centrifugeuse en forme de cuvette, qui est réalisé en particulier sous la forme d'un rotor à angle fixe (10 ; 200 ; 300).
EP17177239.5A 2017-06-21 2017-06-21 Rotor de centrifugeuse doté de joint d'étanchéité Active EP3417943B1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP17177239.5A EP3417943B1 (fr) 2017-06-21 2017-06-21 Rotor de centrifugeuse doté de joint d'étanchéité
CN201880049122.6A CN110997153B (zh) 2017-06-21 2018-06-19 带密封件的离心机转子
US16/625,634 US11471897B2 (en) 2017-06-21 2018-06-19 Centrifuge rotor having seal
JP2019570892A JP6967616B2 (ja) 2017-06-21 2018-06-19 シールを有する遠心分離機ロータ
PCT/EP2018/066307 WO2018234334A1 (fr) 2017-06-21 2018-06-19 Rotor de centrifugation à étanchéité

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP17177239.5A EP3417943B1 (fr) 2017-06-21 2017-06-21 Rotor de centrifugeuse doté de joint d'étanchéité

Publications (2)

Publication Number Publication Date
EP3417943A1 EP3417943A1 (fr) 2018-12-26
EP3417943B1 true EP3417943B1 (fr) 2020-02-12

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EP17177239.5A Active EP3417943B1 (fr) 2017-06-21 2017-06-21 Rotor de centrifugeuse doté de joint d'étanchéité

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US (1) US11471897B2 (fr)
EP (1) EP3417943B1 (fr)
JP (1) JP6967616B2 (fr)
CN (1) CN110997153B (fr)
WO (1) WO2018234334A1 (fr)

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BR112020008652A2 (pt) 2017-11-03 2020-11-10 Takeda Vaccines, Inc. vacinas e composições imunogênicas contra zika e métodos de uso das mesmas
AU2018375173B2 (en) * 2017-11-30 2022-08-25 Takeda Vaccines, Inc. Zika vaccines and immunogenic compositions, and methods of using the same
DE102017130787A1 (de) * 2017-12-20 2019-06-27 Eppendorf Ag Zentrifugenrotor
CN112973975B (zh) * 2021-02-24 2022-08-02 安徽中科中佳科学仪器有限公司 一种自洁净过滤分离的实验室用高速离心机
EP4180132A1 (fr) 2021-11-11 2023-05-17 Eppendorf SE Rotor centrifuge, couvercle de rotor et partie inférieure de rotor
EP4180131A1 (fr) 2021-11-11 2023-05-17 Eppendorf SE Rotor centrifuge, couvercle de rotor et partie inférieure de rotor

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Publication number Publication date
JP6967616B2 (ja) 2021-11-17
EP3417943A1 (fr) 2018-12-26
JP2020524598A (ja) 2020-08-20
WO2018234334A1 (fr) 2018-12-27
CN110997153A (zh) 2020-04-10
US11471897B2 (en) 2022-10-18
US20210187518A1 (en) 2021-06-24
CN110997153B (zh) 2022-04-12

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