EP3001833A1 - Purification de conjugués nanoparticules-anticorps - Google Patents
Purification de conjugués nanoparticules-anticorpsInfo
- Publication number
- EP3001833A1 EP3001833A1 EP15790812.0A EP15790812A EP3001833A1 EP 3001833 A1 EP3001833 A1 EP 3001833A1 EP 15790812 A EP15790812 A EP 15790812A EP 3001833 A1 EP3001833 A1 EP 3001833A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- antibody
- nanoparticle
- free
- mixture
- conjugate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
- A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
- A61K47/6927—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores
- A61K47/6929—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores the form being a nanoparticle, e.g. an immuno-nanoparticle
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
Definitions
- P-dot nanoparticles are highly fluorescent and are useful reporters when conjugated to antibodies or other proteins.
- the purification method can be applied to a wide range of antibody-nanparticle conjugates.
- the antibody is an IgA, IgD, IgE, IgG, and IgM antibody.
- the antibody is an antigen-binding antibody fragment such as, for example, a Fab, F(ab')2, or Fv, or a fusion protein comprising such fragments.
- the antibody is a single-chain antibody, e.g., a scFv, a fusion of the variable regions of the heavy (VH) and light chains (VL) of one or more antibodies.
- the antibody can be recombinant or naturally-occurring.
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Nanotechnology (AREA)
- Water Supply & Treatment (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
Abstract
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462016752P | 2014-06-25 | 2014-06-25 | |
PCT/US2015/037499 WO2015200526A1 (fr) | 2014-06-25 | 2015-06-24 | Purification de conjugués nanoparticules-anticorps |
Publications (3)
Publication Number | Publication Date |
---|---|
EP3001833A1 true EP3001833A1 (fr) | 2016-04-06 |
EP3001833A4 EP3001833A4 (fr) | 2017-03-29 |
EP3001833B1 EP3001833B1 (fr) | 2019-01-23 |
Family
ID=54930215
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP15790812.0A Active EP3001833B1 (fr) | 2014-06-25 | 2015-06-24 | Purification de conjugués nanoparticules-anticorps |
Country Status (4)
Country | Link |
---|---|
US (1) | US20150377869A1 (fr) |
EP (1) | EP3001833B1 (fr) |
CN (1) | CN105431378A (fr) |
WO (1) | WO2015200526A1 (fr) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11000603B2 (en) | 2015-04-14 | 2021-05-11 | Benhealth Biopharmaceutic (Shenzhen) Co., Ltd. | Multi-specific binding conjugate, related pharmaceutical compositions and use |
CN109526227A (zh) | 2016-01-25 | 2019-03-26 | 生物辐射欧洲有限公司 | 数字微生物学 |
WO2017219974A1 (fr) * | 2016-06-22 | 2017-12-28 | 本康生物制药(深圳)有限公司 | Anticorps bispécifique et conjugué d'anticorps pour thérapie tumorale et leurs utilisations |
WO2018063981A1 (fr) | 2016-09-29 | 2018-04-05 | Bio-Rad Laboratories, Inc. | Procédés de purification de conjugués protéine-nanoparticule |
EP3608665A4 (fr) * | 2017-04-07 | 2020-04-22 | Konica Minolta, Inc. | Procédé de fabrication d'un produit purifié de particule intégrée au phosphore modifié par une protéine, procédé de fabrication de liquide de coloration fluorescent, produit purifié de particule intégrée au phosphore modifié par une protéine, et filtre de purification d'un liquide de coloration fluorescent et d'une particule intégrée au phosphore modifiée par une protéine |
JP7431711B2 (ja) | 2020-09-30 | 2024-02-15 | 三洋化成工業株式会社 | 免疫測定方法及び免疫測定用キット |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SI21258A (sl) * | 2002-07-17 | 2004-02-29 | LEK farmacevtska dru�ba d.d. | Stabilni farmacevtski pripravek, ki vsebuje eritropoietin in poloksamerni poliol |
WO2004016160A2 (fr) * | 2002-08-19 | 2004-02-26 | Iowa State University Research Foundation, Inc. | Nanoparticules polymeres redox |
JP4824565B2 (ja) * | 2003-05-21 | 2011-11-30 | アレス トレーディング ソシエテ アノニム | タンパク質溶液のクロマトグラフィー分析の方法 |
AU2006239154A1 (en) * | 2005-04-28 | 2006-11-02 | Ventana Medical Systems, Inc | Nanoparticle conjugates |
EP2176292A1 (fr) * | 2007-07-27 | 2010-04-21 | Pfizer Limited | Procédé de purification d'anticorps |
CN102316858A (zh) * | 2008-02-26 | 2012-01-11 | 阿帕玛生物科技公司 | 修饰过的可调的纳米粒子用于传递治疗,诊断,实验化合物及相关成分用于治疗用药 |
US9040310B2 (en) * | 2010-04-27 | 2015-05-26 | Ventana Medical Systems, Inc. | Antibody-nanoparticle conjugates and methods for making and using such conjugates |
-
2015
- 2015-06-24 US US14/749,022 patent/US20150377869A1/en not_active Abandoned
- 2015-06-24 CN CN201580000855.7A patent/CN105431378A/zh not_active Withdrawn
- 2015-06-24 EP EP15790812.0A patent/EP3001833B1/fr active Active
- 2015-06-24 WO PCT/US2015/037499 patent/WO2015200526A1/fr active Application Filing
Non-Patent Citations (2)
Title |
---|
See also references of WO2015200526A1 * |
TERO SOUKKA ET AL: "Supersensitive Time-resolved Immunofluorometric Assay of Free Prostate-specific Antigen with Nanoparticle Label Technology", CLINICAL CHEMISTRY, AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY, WASHINGTON, DC, vol. 47, no. 7, 1 January 2001 (2001-01-01), pages 1269 - 1278, XP002294914, ISSN: 0009-9147 * |
Also Published As
Publication number | Publication date |
---|---|
EP3001833A4 (fr) | 2017-03-29 |
WO2015200526A1 (fr) | 2015-12-30 |
CN105431378A (zh) | 2016-03-23 |
EP3001833B1 (fr) | 2019-01-23 |
US20150377869A1 (en) | 2015-12-31 |
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