Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
  • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
  • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
  • C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
  • C07D213/78—Carbon atoms having three bonds to hetero atoms, with at the most one bond to halogen, e.g. ester or nitrile radicals
  • C07D213/79—Acids; Esters
  • C07D213/80—Acids; Esters in position 3
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
  • A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
  • A61K8/4926—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having six membered rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
  • A61P17/08—Antiseborrheics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
  • A61P17/10—Anti-acne agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
  • A61P17/14—Drugs for dermatological disorders for baldness or alopecia
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P5/00—Drugs for disorders of the endocrine system
  • A61P5/24—Drugs for disorders of the endocrine system of the sex hormones
  • A61P5/28—Antiandrogens
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q19/00—Preparations for care of the skin

Definitions

• the present invention relates to novel compounds which inhibit lipogenesis.
• Seborrhea is an excessive production of sebum by the sebaceous glands. Seborrhea can have different causes:
• the nervous system emotional stress, nervous tension exacerbating the sebaceous function
• the main cause is of the hormonal type: a hyper activation of the enzyme 5-a reductase causes seborrhea.
• the seat of the manifestations of seborrhea is the medio-facial region (forehead, nose, chin) where the sebaceous glands are the most numerous and the largest. Seborrhea is also present in the scalp where it predominates in the frontal, fronto-temporal and at the top of the skull.
• 5-a-reductase activity is more important in the sebaceous glands of acne-prone skin regions than in non-acne prone regions (Tiboutot et al., J. Invest Dermatol., 1995, 105: 209-14.
• the sebaceous glands are clumps of specialized cells in the skin called "sebocytes”.
• Sebaceous secretion results from a multistep process: the sebum production by the sebocytes during their maturation and their gradual loading into lipid droplets;
• Sebum consists mainly of triglycerides (55%), wax (25%), squalene (15%) and cholesterol esters (5%).
• seborrhea is often associated with androgenetic alopecia. Indeed, the excess of sebum could obstruct the pore of the bulb by which the hair grows and thus contribute to its smothering and, in a second time, to its atrophy.
• hyper-seborrhea promotes the onset of acne and seborrheic dermatitis.
• the excess of sebum in the hair infundibulum represents a favorable environment for the colonization of Propionibacterium acnes as well as that of Malassezia in the scalp.
• the appearance of lesions in acne or seborrheic dermatitis depends on a too intense pro ⁇ inflammatory response, via the innate immunity receptors, with regard to a too important density respectively in P. acnes and in Malassezia.
• an anti-seborrhoeic active agent with an anti-acne agent makes it possible to fight more effectively the occurrence of acne lesions.
• each of R 1, R 2 , R 3 , R 4 represents a hydrogen atom
• R 1 may also represent a halogen atom or an aryl, heteroaryl, alkenyl or acetylenyl radical.
• aryl radical in the sense of the present invention one or more aromatic rings each having from 5 to 8 carbon atoms, which may be contiguous or fused, substituted or not.
• the aryl groups may be phenyl or naphthyl groups and the substituents of halogen atoms, Ci groups -C 4 - alkoxy, Ci to C 4 alkyl groups or nitro.
• heteroaryl radical means an aryl radical as defined above in which one or more carbon atoms has been substituted by a heteroatom such as, for example, nitrogen, oxygen or sulfur, such as pyridine, pyrimidine, imidazole, indole, furan or thiophene.
• alkenyl radical is understood to mean a vinyl radical which may or may not be substituted by a Ci to C 4 -alkyl radical.
• acetylenyl radical is understood to mean an alkynyl radical which may or may not be substituted by a Ci to C 4 -alkyl radical.
• the compounds of the invention are the following:
• glyceryl laurate - nicotinic acid either 3- (dodecanoyloxy) -2-hydroxypropyl nicotinate
• glyceryl laurate - nipecotic acid either 3- (dodecanoyloxy) -2-hydroxypropyl piperidine-3-carboxylate
• the compounds of formula I reduce the biosynthesis of the major constituents of sebum, in particular the fatty acids constituting the triglycerides.
• a remarkable feature of the compounds of the present invention is the improved bioavailability of these compounds, i.e. their ability to penetrate through from the skin to reach the sebaceous gland.
• their bioavailability is improved in view of their lipophilic gain: the liposoluble ester form of the derivatives of general formula I has a better bioavailability compared with water-soluble glyceryl laurate.
• Tel-E6E7 (courtesy of Dr. Stephen Lyle, University of Massachusetts, USA) is a human epithelial line derived from stem cells derived from the bulb of hair follicles. This line, immortalized by the expression of the E6 / E7 genes of the HPV 16 virus, is a multipotent epithelial line capable of differentiating into sebocytes under particular culture conditions (Multi-potentiality of a new immortalized epithelial stem cell line derived from human hair follicles, Roh C, Roche M., Guo Z., Photopoulos C, Tao Q., Lyle S., In Vitro Cell Dev Biol Anim, July-August 2008, 44 (7): 236-44).
• the differentiation into sebocytes of the Tel-E6E7 line was carried out according to the conditions described by S. Lyle.
• the cells are seeded in 24-well plates with a density of 25,000 cells / cm 2 in DME medium supplemented with 46% HamF12 medium, 6% fetal calf serum (FCII, Hyclone), 2% human serum. and 10 nM EGF.
• FCII fetal calf serum
• FCII fetal calf serum
• the differentiation of the sebocytes is maintained for 7 days.
• the culture medium and the test derivatives are renewed every 2-3 days.
• the production of intracellular lipids is performed using the quantification of the Nil Red marker (Adipored kit, Lonza) and according to the instructions given by the supplier.
• the amount of fluorescence measured is proportional to the amount of accumulated intracellular neutral lipids. All conditions tested were duplicated and repeated three times.
• the inventors have studied the variations in lipid levels produced by the sebocytes after 7 days of differentiation in the presence of testosterone treated with the derivatives of the present invention.
• FIG. 1 represents the evaluation of the effect of the esters of general formula I with regard to the production of intracellular lipids by differentiated sebocytes (Tel-E6E7 line) in the presence of testosterone and of other supplements.
• the invention relates to the use of a compound of formula I as a medicament or as a cosmetic active ingredient.
• the medicament is intended for the treatment of acne, seborrheic dermatitis or androgenetic alopecia.
• the invention relates to the cosmetic use of a compound of formula I for the treatment of seborrhea.
• the invention relates to a composition
• a composition comprising as active principle an anti-seborrhoeic compound of formula I as defined above in association with at least one pharmaceutically or cosmetically acceptable excipient.
• the invention relates to a composition for its use as a medicament.
• the invention relates to a composition for use in the treatment of acne, seborrheic dermatitis, and androgenetic alopecia.
• the compounds of formula I as defined above will be associated in said composition with at least one other active agent promoting their action in the indications of androgenetic acne and alopecia.
• composition for the treatment of acne further comprises an active ingredient anti-acne.
• composition intended for the treatment of alopecia further comprises an anti-hair loss active agent.
• this relates to the cosmetic use of a composition for the treatment of seborrhea.
• the acyl chloride in hydrochloride form (1.5 eq) is suspended in dichloromethane (20 ml per 1 g of glyceryl laurate), and the pyridine is added. After stirring for 10 minutes, the glyceryl laurate (1 eq) is added to the reaction medium which has become homogeneous. The reaction medium is stirred at room temperature for 24 hours. The progress of the reaction is monitored by TLC (eluent: DCM / MeOH: 95/5, revealing with phosphomolydic acid).
• reaction medium is hydrolysed with water (10 ml per 1 g of glyceryl laurate) and the aqueous phase is extracted three times with dichloromethane (10 ml per 1 g of glyceryl laurate).
• dichloromethane 10 ml per 1 g of glyceryl laurate.
• the organic phases are combined, washed once with water, dried over magnesium sulfate, filtered and then concentrated under reduced pressure.
• esters are isolated after salification and recrystallizations. For some of them, it was necessary to carry out a purification by chromatography on silica gel.
• a / tri-ester B / glyceryl laurate is solubilized in ethyl ether (10 V).
• the solution is cooled to 0 ° C using an ice bath to pour a solution of 2N hydrochloric acid in ethyl ether (3 eq). A non-filterable precipitate is formed. After stirring for 30 minutes, the reaction medium is concentrated under reduced pressure to provide a white wax.
• the white wax is recrystallized from acetonitrile (10 V).
• the white solid formed is filtered and dried under vacuum for a few hours.
• the hydrochloride formed is analyzed by LCUV for purity.
• the 1-nicotinoyloxy derivative of glyceryl laurate A will be recrystallized until a purity greater than 99% is obtained.
• the sequence of recrystallizations makes it possible to reach a UV purity greater than 99%.
• Anal. ELEM. % (C 2 iH 3 2BrNO 5 ): Theorem. C, 55, 02, H, 7.04,, 3.06; exp. C, 55.05, H, 7.04, N, 3.06.
• Step 1
• the nipecotinic acid (3.7 g) is dissolved in a 3N sodium hydroxide solution (30 ml). The solution is cooled to 0 ° C in an ice bath. Benzyl chloroformate (5.8 ml) is added at 0 ° C in portions, alternating with 3N sodium hydroxide solution (9.3 ml) over a period of 45 minutes. At the end of the casting, the reaction medium is pale yellow and the temperature is 3 ° C. The ice bath is removed and the medium The reaction mixture is stirred at room temperature overnight.
• the aqueous phase is then extracted once with ethyl ether and then acidified to pH 1 with a 3N hydrochloric acid solution.
• the acidified aqueous phase is extracted three times with ethyl ether.
• the ethereal phase is washed successively with 1N hydrochloric acid solution and then twice with a saturated solution of sodium chloride before being dried over magnesium sulfate, filtered and then concentrated under reduced pressure.
• the yellow oil obtained (7.35 g, 98%) is used in the next step without purification step.
• step 1 In a three-necked flask of 1 L under nitrogen flow and with magnetic stirring, the acid obtained in step 1 (3.5 g) is dissolved in dichloromethane (440 ml). EDCI.HCl (2.81 g) and DMAP (0.81 g) are added. The colorless solution is stirred at room temperature for 15 minutes before adding the glyceryl laurate (10.95 g). The reaction medium is left stirring at ambient temperature for the night and then hydrolysed with water (200 ml). The organic phase is separated and washed twice with saturated sodium hydrogencarbonate solution, then dried over magnesium sulfate, filtered, and finally concentrated under reduced pressure to provide a white waxy solid (15.5 g).
• GOLD 120 g prepainted column chromatography (heptane / ethyl acetate gradient of 0 to 30% in 13 column volumes (CV), heptane / ethyl acetate plate of 70/30 in 5HP) makes it possible to separate the expected ester of excess glyceryl laurate and triester (3.5% detected in LCMS). This fraction (2.64 g) is used in the next step.

Landscapes

• Health & Medical Sciences (AREA)
• Organic Chemistry (AREA)
• Chemical & Material Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Veterinary Medicine (AREA)
• Public Health (AREA)
• General Health & Medical Sciences (AREA)
• Animal Behavior & Ethology (AREA)
• Medicinal Chemistry (AREA)
• Pharmacology & Pharmacy (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• Engineering & Computer Science (AREA)
• General Chemical & Material Sciences (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Dermatology (AREA)
• Endocrinology (AREA)
• Diabetes (AREA)
• Birds (AREA)
• Epidemiology (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
• Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
• Pyridine Compounds (AREA)
• Cosmetics (AREA)
• Hydrogenated Pyridines (AREA)

Applications Claiming Priority (2)

Publications (1)

Family

ID=47324157

Family Applications (1)

Country Status (13)

Families Citing this family (4)

Family Cites Families (12)

• 2011
  • 2011-12-08 FR FR1161341A patent/FR2983858B1/fr not_active Expired - Fee Related
• 2012
  • 2012-12-10 CA CA2857973A patent/CA2857973A1/fr not_active Abandoned
  • 2012-12-10 AU AU2012350282A patent/AU2012350282A1/en not_active Abandoned
  • 2012-12-10 US US14/363,289 patent/US9181189B2/en not_active Expired - Fee Related
  • 2012-12-10 KR KR1020147018421A patent/KR20140101834A/ko not_active Application Discontinuation
  • 2012-12-10 CN CN201280059828.3A patent/CN103974937A/zh active Pending
  • 2012-12-10 MX MX2014006699A patent/MX2014006699A/es unknown
  • 2012-12-10 EP EP12798298.1A patent/EP2788327A1/fr not_active Withdrawn
  • 2012-12-10 JP JP2014545301A patent/JP6113744B2/ja not_active Expired - Fee Related
  • 2012-12-10 WO PCT/EP2012/074891 patent/WO2013083825A1/fr active Application Filing
  • 2012-12-10 BR BR112014013719A patent/BR112014013719A8/pt not_active IP Right Cessation
  • 2012-12-10 RU RU2014126394A patent/RU2014126394A/ru not_active Application Discontinuation
• 2014
  • 2014-10-14 HK HK14110259A patent/HK1197057A1/xx unknown

Also Published As

Similar Documents

Legal Events