EP2625290A4 - Détection par pcr en temps réel de polymorphismes nucléotidiques simples - Google Patents

Détection par pcr en temps réel de polymorphismes nucléotidiques simples

Info

Publication number
EP2625290A4
EP2625290A4 EP11830866.7A EP11830866A EP2625290A4 EP 2625290 A4 EP2625290 A4 EP 2625290A4 EP 11830866 A EP11830866 A EP 11830866A EP 2625290 A4 EP2625290 A4 EP 2625290A4
Authority
EP
European Patent Office
Prior art keywords
real time
time pcr
single nucleotide
nucleotide polymorphisms
pcr detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP11830866.7A
Other languages
German (de)
English (en)
Other versions
EP2625290A2 (fr
Inventor
Jason Opdyke
John Harvey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hanwha Techwin Co Ltd
Original Assignee
Samsung Techwin Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/158,593 external-priority patent/US20120088246A1/en
Application filed by Samsung Techwin Co Ltd filed Critical Samsung Techwin Co Ltd
Publication of EP2625290A2 publication Critical patent/EP2625290A2/fr
Publication of EP2625290A4 publication Critical patent/EP2625290A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/327—RNAse, e.g. RNAseH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP11830866.7A 2010-10-04 2011-10-04 Détection par pcr en temps réel de polymorphismes nucléotidiques simples Withdrawn EP2625290A4 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US38941210P 2010-10-04 2010-10-04
US39070110P 2010-10-07 2010-10-07
US13/158,593 US20120088246A1 (en) 2010-10-07 2011-06-13 Real time pcr detection of single nucleotide polymorphisms
PCT/KR2011/007294 WO2012046981A2 (fr) 2010-10-04 2011-10-04 Détection par pcr en temps réel de polymorphismes nucléotidiques simples

Publications (2)

Publication Number Publication Date
EP2625290A2 EP2625290A2 (fr) 2013-08-14
EP2625290A4 true EP2625290A4 (fr) 2014-04-02

Family

ID=45928196

Family Applications (1)

Application Number Title Priority Date Filing Date
EP11830866.7A Withdrawn EP2625290A4 (fr) 2010-10-04 2011-10-04 Détection par pcr en temps réel de polymorphismes nucléotidiques simples

Country Status (5)

Country Link
EP (1) EP2625290A4 (fr)
JP (1) JP2013545442A (fr)
KR (1) KR20120046018A (fr)
CN (1) CN103154271A (fr)
WO (1) WO2012046981A2 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117106868A (zh) * 2013-11-26 2023-11-24 杭州联川基因诊断技术有限公司 一种扩增靶核酸的试剂盒和方法
WO2015095825A1 (fr) 2013-12-20 2015-06-25 Biomed Valley Discoveries, Inc. Traitements de cancer utilisant des combinaisons d'inhibiteurs erk et mek de type 2
CA3240745A1 (fr) 2013-12-20 2015-06-25 Biomed Valley Discoveries, Inc. Traitement du cancer faisant appel a des associations d'inhibiteurs de l'erk et de la raf
EP3082423A4 (fr) 2013-12-20 2017-09-20 Biomed Valley Discoveries, Inc. Traitements anticancéreux utilisant des combinaisons d'inhibiteurs de cdk et d'erk
EP3082957B1 (fr) 2013-12-20 2022-04-06 Biomed Valley Discoveries, Inc. Traitements du cancer utilisant des combinaisons des inhibiteurs de la voie pi3k/akt et de l'erk
KR101598398B1 (ko) * 2014-01-08 2016-02-29 (주)제노텍 5''-플랩 엔도뉴클레이즈 활성의 억제를 이용하여 실시간 중합효소 연쇄반응으로 돌연변이 유전자를 검사하는 방법
CN104911265A (zh) * 2015-06-03 2015-09-16 辽宁省农业科学院蔬菜研究所 同时检测番茄抗病基因Ty-3a和Tm-2a的双重PCR方法
WO2018026039A1 (fr) * 2016-08-05 2018-02-08 주식회사 하임바이오텍 Kit et procédé de détection de polymorphisme nucléotidique simple
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
CN108588252A (zh) * 2018-03-29 2018-09-28 杭州泰熙生物技术有限公司 一种寄生虫检测试剂盒及其检测方法
KR102275038B1 (ko) * 2019-12-11 2021-07-08 (주) 제노텍 대립 유전자의 구분성을 높이는 pcr 방법 및 pcr 킷트
CN113215163B (zh) * 2021-05-12 2023-05-12 苏州海苗生物科技有限公司 一种特异性扩增目的基因的分子锁及应用
CN117089605B (zh) * 2023-05-08 2024-05-03 中国科学院苏州生物医学工程技术研究所 一种基于fq-rca的rna等温实时基因分型方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763181A (en) * 1994-12-30 1998-06-09 Georgetown University Continous fluorometric assay for detecting nucleic acid cleavage

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6558929B2 (en) * 2000-09-15 2003-05-06 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forshung E.V. PCR reaction mixture for fluorescence-based gene expression and gene mutation analyses
US7157228B2 (en) * 2002-09-09 2007-01-02 Bioarray Solutions Ltd. Genetic analysis and authentication
US20060121487A1 (en) * 2002-12-27 2006-06-08 David Alland Method for single nucleotide polymorphism detection
US7824861B2 (en) * 2008-07-10 2010-11-02 National Tsing Hua University Method for quantitative analysis of transcripts with nucleotide polymorphism at specific site

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763181A (en) * 1994-12-30 1998-06-09 Georgetown University Continous fluorometric assay for detecting nucleic acid cleavage

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
"CycleavePCR (TM) Core Kit", PRODUCT INFORMATION, 26 September 2008 (2008-09-26), XP055103372, Retrieved from the Internet <URL:http://old.takara.co.kr/pds/shop/product/manual/CY501_e.pdf> [retrieved on 20140220] *
HARVEY J J ET AL: "Characterization and applications of CataCleave probe in real-time detection assays", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS INC, NEW YORK, vol. 333, no. 2, 15 October 2004 (2004-10-15), pages 246 - 255, XP004573012, ISSN: 0003-2697, DOI: 10.1016/J.AB.2004.05.037 *
HIDETAKE ESAKI ET AL: "Rapid detection of quinolone-resistant Salmonella by real time SNP genotyping", JOURNAL OF MICROBIOLOGICAL METHODS, vol. 58, no. 1, 1 July 2004 (2004-07-01), pages 131 - 134, XP055103125, ISSN: 0167-7012, DOI: 10.1016/j.mimet.2004.03.010 *
JOHN J. HARVEY ET AL: "SNP analysis using catacleave probes", JOURNAL OF CLINICAL LABORATORY ANALYSIS, vol. 22, no. 3, 1 January 2008 (2008-01-01), pages 192 - 203, XP055098111, ISSN: 0887-8013, DOI: 10.1002/jcla.20240 *

Also Published As

Publication number Publication date
JP2013545442A (ja) 2013-12-26
KR20120046018A (ko) 2012-05-09
WO2012046981A3 (fr) 2012-07-19
WO2012046981A2 (fr) 2012-04-12
CN103154271A (zh) 2013-06-12
EP2625290A2 (fr) 2013-08-14

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