EP2567092A1 - Elément microfluidique, en particulier micropompe péristaltique, et son procédé de réalisation - Google Patents
Elément microfluidique, en particulier micropompe péristaltique, et son procédé de réalisationInfo
- Publication number
- EP2567092A1 EP2567092A1 EP11709089A EP11709089A EP2567092A1 EP 2567092 A1 EP2567092 A1 EP 2567092A1 EP 11709089 A EP11709089 A EP 11709089A EP 11709089 A EP11709089 A EP 11709089A EP 2567092 A1 EP2567092 A1 EP 2567092A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- substrate
- elastic membrane
- microfluidic component
- fluidic
- microfluidic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000002572 peristaltic effect Effects 0.000 title claims abstract description 22
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 11
- 239000000758 substrate Substances 0.000 claims abstract description 97
- 239000012528 membrane Substances 0.000 claims abstract description 60
- 239000012530 fluid Substances 0.000 claims abstract description 47
- 239000003153 chemical reaction reagent Substances 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 7
- 238000003860 storage Methods 0.000 claims description 7
- 239000004020 conductor Substances 0.000 claims description 5
- 238000003466 welding Methods 0.000 claims description 5
- 229920000089 Cyclic olefin copolymer Polymers 0.000 claims description 4
- 239000004793 Polystyrene Substances 0.000 claims description 4
- 150000001925 cycloalkenes Chemical class 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 4
- 229920000515 polycarbonate Polymers 0.000 claims description 4
- 239000004417 polycarbonate Substances 0.000 claims description 4
- 229920002223 polystyrene Polymers 0.000 claims description 4
- 239000010703 silicon Substances 0.000 claims description 4
- 229910052710 silicon Inorganic materials 0.000 claims description 4
- 229920002725 thermoplastic elastomer Polymers 0.000 claims description 3
- 239000002131 composite material Substances 0.000 claims 1
- 238000005086 pumping Methods 0.000 description 48
- 239000007788 liquid Substances 0.000 description 17
- 229920000642 polymer Polymers 0.000 description 5
- 230000006870 function Effects 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000004205 dimethyl polysiloxane Substances 0.000 description 3
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 3
- 229920001971 elastomer Polymers 0.000 description 3
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 3
- 229920000307 polymer substrate Polymers 0.000 description 3
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 2
- 229920006362 Teflon® Polymers 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000005530 etching Methods 0.000 description 2
- 238000001746 injection moulding Methods 0.000 description 2
- 230000003446 memory effect Effects 0.000 description 2
- 230000005499 meniscus Effects 0.000 description 2
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 2
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 2
- 229920000728 polyester Polymers 0.000 description 2
- 239000005297 pyrex Substances 0.000 description 2
- 239000005060 rubber Substances 0.000 description 2
- 239000005062 Polybutadiene Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000000806 elastomer Substances 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000004049 embossing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003891 environmental analysis Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000003475 lamination Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000000206 photolithography Methods 0.000 description 1
- 229920005559 polyacrylic rubber Polymers 0.000 description 1
- 229920002857 polybutadiene Polymers 0.000 description 1
- -1 polydimethylsiloxane Polymers 0.000 description 1
- 229920006254 polymer film Polymers 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000000930 thermomechanical effect Effects 0.000 description 1
- 229920001169 thermoplastic Polymers 0.000 description 1
- 239000004416 thermosoftening plastic Substances 0.000 description 1
Classifications
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B43/00—Machines, pumps, or pumping installations having flexible working members
- F04B43/02—Machines, pumps, or pumping installations having flexible working members having plate-like flexible members, e.g. diaphragms
- F04B43/04—Pumps having electric drive
- F04B43/043—Micropumps
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B43/00—Machines, pumps, or pumping installations having flexible working members
- F04B43/02—Machines, pumps, or pumping installations having flexible working members having plate-like flexible members, e.g. diaphragms
- F04B43/06—Pumps having fluid drive
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B43/00—Machines, pumps, or pumping installations having flexible working members
- F04B43/12—Machines, pumps, or pumping installations having flexible working members having peristaltic action
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B9/00—Piston machines or pumps characterised by the driving or driven means to or from their working members
- F04B9/08—Piston machines or pumps characterised by the driving or driven means to or from their working members the means being fluid
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B43/00—Machines, pumps, or pumping installations having flexible working members
- F04B43/12—Machines, pumps, or pumping installations having flexible working members having peristaltic action
- F04B43/14—Machines, pumps, or pumping installations having flexible working members having peristaltic action having plate-like flexible members
Definitions
- Microfluidic component in particular peristaltic micropump, and method for its production
- the present invention relates to a microfluidic component, in particular a peristaltic micropump, and a method for its production.
- Macroscopic peristaltic pumps in radial and linear design are known from medical technology and chemical analysis.
- a flexible hose is compressed from the outside so that it comes to a directed displacement of the liquid contained therein.
- peristaltic micropumps offer the advantage of a greatly simplified production because there are no check valves
- Nguyen et al. describe that the peristaltic micropumps require at least three pumping chambers connected by channels, each pumping chamber being bounded on one side by a pumping membrane
- a wave-like actuation of the pumping chambers can be used to pump a fluid in a desired direction
- micropumps can be used for the transport of fluids in microfluidic systems.
- Microfluidic systems are used, for example, in the form of miniaturized analysis systems, so-called ⁇ (Miniaturized Total Analysis System) or lab-on-chip systems or as microreactors. They find particular applications in molecular diagnostics, biotechnology, analytical, pharmaceutical and clinical chemistry, environmental analysis and food chemistry.
- the subject matter of the present invention is a microfluidic component, in particular a peristaltic micropump, comprising at least one layer structure comprising a fluidic substrate, a drive substrate and an elastic membrane arranged therebetween, wherein the fluidic substrate has a fluid chamber on its side adjacent to the elastic membrane, the drive substrate , At least on its side adjacent to the elastic membrane side, at least two independent Aktuleiterskomponenten each having at least partially disposed opposite the fluid chamber and can act on the elastic membrane and the Aktuiansskomponenten, optionally by one or more actuators, are independently actuated.
- the at least three pumping chambers of the known peristaltic micropumps are replaced by a single main chamber. This advantageously results in a minimal fluidic resistance as well as an increased pumping rate. At the same time, the dead volume otherwise given by the connection channels between the pumping chambers can be eliminated.
- the fluidic substrate is essentially plate-shaped, for example designed in the form of a layer.
- the fluid chamber is formed as a recess in the fluidic substrate, which is closed by the elastic membrane on the side adjacent to this membrane.
- the fluidic substrate is connected to the elastic membrane.
- the fluid chamber according to the invention is also referred to as a pumping chamber or reservoir.
- the drive substrate Opposite the fluidic substrate and separated therefrom, at least in the region of the pumping chamber, by an elastic membrane is the drive substrate, which is likewise connected to the elastic membrane. This results in a sandwich-like structure of the microfluidic component.
- the drive substrate may also be substantially plate-shaped.
- the at least two actuation components may comprise in the drive substrate, for example, actuation chambers, which may be formed as recesses, which are closed by the elastic membrane on the side adjacent to this membrane.
- the Aktuleitershuntn may for example be separated by webs. The webs are expediently designed as narrow as possible in relation to the Aktu michshuntn.
- the drive substrate can also be referred to as cover or cap of the microfluidic component.
- the Aktuiansskomponenten can according to the invention act directly or indirectly on the elastic membrane.
- the elastic membrane according to the invention can be realized in particular as a continuous film-shaped film.
- the simplified elastic membrane results in a simplified production, improved mechanical strength and a lower risk of leaks.
- the elastic membrane is also referred to as a pumping membrane.
- microfluidic component according to the invention in particular a microfluidic pump, are a higher possible pumping rate relative to the size and a minimized dead volume.
- the minimized dead volume can in particular also ensure the pumpability of gases and keep the required amounts of possibly expensive samples and reagents low.
- the actuation of the actuation components used in the microfluidic component can be based, for example, on electromagnetic, piezoelectric based on electrical, hydraulic or pneumatic action principles.
- the corresponding actuation components and / or actuators used for this purpose usually have the advantage that they can be easily implemented and offer a strong drive.
- Other possible actuations according to the invention can also be based, for example, on electrostatic, electromagnetic, thermopneumatic or thermomechanical principles of action.
- the actuation components can comprise actuation chambers which can be subjected to a pneumatic or hydraulic pressure, for example via supply ducts.
- thermopneumatically a pressure can be generated in the Aktuleitershuntn, for example by heating a gas contained therein or evaporation of a liquid. If the pressure in the respective actuation chamber exceeds the pressure in the fluidic plane, the elastic membrane is deflected into the volume of the fluid chamber and the respective fluid, for example a liquid reagent, displaced. For example, by a Lauflichtianos Aktutechniksmuster a directed fluid flow can be achieved.
- the component according to the invention can, in particular for controlling the actuation, furthermore a control device.
- the drive substrate may comprise integrated electromagnetic, electrostatic, piezoelectric and / or shape memory effect actuation components which may be independently actuated.
- Such actuators can act directly on the membrane and can optionally be produced simultaneously with the microfluidic component and advantageously allow a simplified miniaturization.
- the actuation substrate instead of the actuation chambers, has an opening through which external electromagnetic, electrostatic, piezoelectric or shape memory effect actuators, if appropriate, can act on the membrane by means of a plunger.
- the fluidic substrate and / or the drive substrate may be structured Polymer substrate, in particular of polycarbonate, polystyrene, polydimethylsiloxane or a cyclo-olefin copolymer, or designed as a glass or silicon substrate.
- Polymer substrate in particular of polycarbonate, polystyrene, polydimethylsiloxane or a cyclo-olefin copolymer, or designed as a glass or silicon substrate.
- made of polymers can be advantageously resorted to suitable and established methods such as hot stamping or injection molding.
- the preparation of polymers is usually also inexpensive.
- the recesses can also be subsequently formed for example by photolithography and etching or by milling in the substrates.
- the fluidic substrate and the drive substrate can independently of one another have a layer thickness of 300 ⁇ m to 10 mm and / or a base area of 5 mm ⁇ 5 mm to 200 mm ⁇ 200 mm, for example of 15 mm ⁇ 30 mm.
- the fluidic substrate and drive substrate may have the same footprint and terminate flush with each other.
- the fluid chamber according to the invention for example, a length L1 of
- the height of the fluid chamber T2 can be, in particular,> 100 ⁇ m to ⁇ 1000 ⁇ m, for example 250 ⁇ m or 500 ⁇ m.
- the Aktuiansshuntn can, for example, a length L2 of> 3 mm to
- ⁇ 1000 ⁇ for example, 250 ⁇ or 500 ⁇ amount.
- the elastic membrane can be designed as a polymer film, in particular with a thickness of> 0.01 ⁇ m to 200 ⁇ m, for example with a thickness of 100 ⁇ m.
- the elastic membrane may also be made thicker or thinner.
- the elastic membrane may be formed from a thermoplastic, in particular weldable, polymer, in particular from a thermoplastic elastomer.
- a weldable polymer as an elastic membrane, this can advantageously, in addition to the pumping and displacement function for a fluid, simultaneously serve as connection and sealing of the layer structure according to the invention, for example three-layer structure.
- the use of a weldable polymer further allows easy connection of the substrates to the elastic membrane, for example by laser welding, ultrasonic welding or thermo-compression welding.
- the elastic membrane is formed from a non-thermoplastic elastomer.
- suitable elastomers are silicone elastomers, polyurethanes, rubbers such as polyacrylic rubber, styrene rubber, butadiene rubber or mixtures thereof.
- the connection to the fluidic substrate and the drive substrate in this case can be done, for example, by an adhesive technique, for example, a lamination.
- the fluid chamber may have rounded side walls.
- the elastic membrane deflected downwards into the fluid chamber for example the pumping membrane, can thereby better fit against the fluid chamber and the fluidic resistance in the actuated state is increased.
- a reflux of a fluid can advantageously be prevented and the pumping rate can be increased.
- the dead volume is reduced by the rounding.
- the fluid chamber may have phase conductors (phaseguides) arranged on its bottom.
- Phase conductors are capillary pressure barriers, for example, edges or other structures that act perpendicular to the direction of movement of a gas, such as air, liquid meniscus. The meniscus then aligns itself with the phase conductor and eventually overcomes the barrier given, for example, by edges. In this way, a controlled, bubble-free filling and emptying with liquids can be achieved.
- the microfluidic component according to the invention as a reservoir for the pre-storage of liquid reagents, for example in a lab-on-chip system.
- the phase conductors can allow even more accurate dimensioning and pre-storage of a defined amount of liquid, as well as the substantially residue-free controlled emptying of the reservoir.
- the chamber in the fluidic substrate for example, completely filled with a liquid reagent.
- a liquid reagent for example, a liquid reagent.
- the emptying of the reservoir can take place, for example, by first opening the valve at the outlet of the main chamber and then actuating the actuation chambers sequentially.
- the fluidic substrate and / or the drive substrate may have further microfluidic structures and / or microfluidic components, for example chambers, mixers, valves.
- the pumping or reservoir function of the microfluidic component can be further supported and / or the microfluidic component can simultaneously take on additional functions associated with the additional components.
- these additional structures and / or components can be made at least partially simultaneously with the pumping chamber or the actuation chambers.
- the invention further relates to a method for producing a microfluidic component, in particular a microfluidic peristaltic pump.
- the fluidic substrate and the drive substrate can in particular be produced by microtechnological methods and provided for the further production of the microfluidic component.
- a plate-shaped or sheet-shaped fluidic substrate and / or as a driving substrate a glass substrate, a silicon substrate, a printed circuit board substrate or a
- Polymer substrate in particular a pyrex substrate, a Teflon substrate, a polystyrene substrate, a polycarbonate substrate, a substrate of a cyclo-olefin copolymer, a polyester substrate or a PDMS substrate, or by injection molding or deep etching or embossing, in particular hot stamping, structured substrate, for example a structured glass substrate,
- Silicon substrate or polymer substrate in particular a pyrex substrate, a teflon substrate, a polystyrene substrate, a polycarbonate substrate, a substrate of a cyclo-olefin copolymer, a polyester substrate or a PDMS substrate.
- the invention also relates to the use of a microfluidic component in a lab-on-chip system.
- the microfluidic component can be used as a peristaltic micropump.
- a microfluidic component according to the invention can be used in a lab-on-chip system as a reservoir for pre-storage, in particular of liquid reagents. The volume of the fluid chamber then forms the fluid reservoir.
- the microfluidic component according to the invention can therefore be used for the pre-storage and metering and / or for the transport of fluids, for example liquid reagents.
- FIG. 1 is a schematic oblique plan view of an inventive microfluidic component, in particular a peristaltic pump
- FIG. 2 shows a schematic plan view of the embodiment of the microfluidic component shown in FIG. 1, FIG.
- FIG. 3 shows a schematic cross-section along the line A-A 'through FIG. 1
- FIG. 4 shows a simplified schematic plan view of a microfluidic component according to the invention
- FIG. 5 shows a schematic cross section along the line B-B 'through FIG. 4
- FIG. 6 shows a schematic plan view of an embodiment of a microfluidic reservoir according to the invention
- FIG. 7 shows a schematic cross section along the line C-C through FIG. 6 embodiment of a microfluidic reservoir according to the invention
- FIG. 8 shows a simplified schematic plan view of a microfluidic component according to the invention with three actuation components
- Fig. 9 is a schematic cross-section along the line DD ' through the embodiment shown in Fig. 7.
- FIG. 1 shows a microfluidic component 1 according to the invention which has a fluidic substrate 2, a drive substrate 4 and an elastic membrane 3 sandwiched between these substrates.
- the fluidic substrate 2 adjoins the elastic membrane 3 and is connected to this area.
- the elastic membrane 3 is also referred to as pumping membrane according to the invention.
- the fluidic substrate 2 has on the side adjacent to the elastic membrane 3 side a recess 5, which is also referred to as a pumping chamber.
- the at least two pumping chambers are combined to form a single main pumping chamber 5.
- the dead volume can be minimized by the construction according to the invention with only one pumping chamber 5, in particular by the associated elimination of connecting channels between the pumping chambers.
- the driving substrate 4 also abuts and is connected to the elastic membrane 3.
- the drive substrate 4 has on its side adjacent to the elastic membrane 3 three recesses 6a, 6b, 6c, which are also referred to as Aktu réelleshuntn.
- the actuation chambers 6a, 6b, 6c which are arranged one behind the other in the flow direction are provided by webs
- actuation chambers are connected to supply channels 8a, 8b, 8c and can be acted on and actuated via inlet openings 9a, 9b, 9c in the drive substrate 4, for example with a hydraulic or pneumatic pressure by an external pressure device (not shown). Exceeds the pressure in the respective
- Actuating chamber 6a, 6b, 6c the pressure in the fluidic plane 2, the pumping membrane 3 is deflected into the volume of the pumping chamber 5 into and displaces the respective fluid.
- a directed fluid flow can be achieved.
- a fluid can be fed through an inlet 10a via a supply channel 1 1 a in the pumping chamber 5 and led out through an outlet 10b via a feed line 1 1 b from the pumping chamber, in particular be pumped out by the previously described actuation of Aktuiansshuntn 6a, 6b, 6c , Further particularly suitable Aktutechniksmuster for a microfluidic peristaltic pump according to the invention with a fluid chamber 5 and three
- Actuation chambers 6a, 6b, 6c are reproduced below in Examples 1 to 6.
- FIG. 2 shows the microfluidic component 1 according to the invention shown in FIG. 1 in a schematic plan view of the drive substrate 4 with its
- FIG. 3 shows a schematic cross section along the line AA 'through the microfluidic component 1 according to the invention shown in FIG. 2.
- the height of the Aktu réelleshuntn 6 T1 according to the invention in particular> 100 ⁇ to ⁇ 1000 ⁇ , for example, 250 ⁇ or 500 ⁇ , amount.
- the fluid chamber 5 according to the invention a height T2 of> 100 ⁇ to ⁇ 1000 ⁇ , for example, 250 ⁇ or 500 ⁇ have.
- the pumping chamber 5 has in this embodiment rounded side walls, whereby advantageously, deflected into the volume of the fluid chamber, elastic membrane, such as pumping membrane, can create better to the fluid chamber walls. This allows the fluidic Resistance can be increased in the actuated state and advantageously prevents backflow of a fluid and the pumping rate can be increased. In addition, the dead volume is reduced by the rounding of the side walls.
- FIG. 4 shows a simplified schematic plan view of a microfluidic component 1, in particular a peristaltic pump.
- the fluidic substrate 2, the elastic membrane 3 and the drive substrate 4 are not shown in this illustration for the sake of clarity.
- FIG. 3 shows once again that according to the invention only one pumping chamber 5 is provided.
- the pumping chamber 5 can be filled and emptied through the supply channels 1 1 a and 1 1 b.
- a fluid can be fed, for example through the channel 1 1 a in the pumping chamber 5, flow through them and leave through the channel 1 1 b the pumping chamber 5 again.
- the generation of a directed fluid flow can be effected by the controlled sequential actuation of the actuation chambers 6a, 6b, 6c.
- the actuation chambers 6a, 6b, 6c can be acted on and actuated via the supply channels 8a, 8b, 8c, for example, with a pneumatic or hydraulic pressure.
- the Aktuiansshuntn 6a, 6b, 6c extend beyond the pumping chamber 5 in this embodiment laterally, perpendicular to the flow direction, and do not close, as shown in Figure 1, with the pumping chamber 5 flush.
- Fig. 5 shows a schematic cross section along the line B-B 'of Fig. 3.
- the fluidic substrate 2, the elastic membrane 3 and the driving substrate 4 are shown as a three-layer structure.
- the figure illustrates that only one pumping chamber 5 is arranged essential to the invention in the fluidic substrate, whereby a minimized dead volume is advantageously provided in comparison with the prior art.
- the controlled sequential actuation of the Aktu réelleshuntn 6a, 6b, 6c for example, pneumatic or hydraulic pressure.
- FIG. 6 shows a schematic plan view of an embodiment of a microfluidic component 1 according to the invention as a reservoir, as can be used, for example, for the preliminary storage of liquid reagents in a microfluidic lab-on-chip system.
- the fluid chamber 5 is used as storage volume, ie as a reservoir.
- the microfluidic component according to the invention may for example have a length L1 of> 15 mm to ⁇ 30 mm, for example 20 mm, and / or a width W of> 5 mm to ⁇ 15 mm, for example 10 mm.
- the height of the fluid chamber T2 according to the invention can be in particular> 100 ⁇ to ⁇ 1000 ⁇ .
- the microfluidic component 1 can furthermore have valves 14, 15 and 16. Through the inlet 10a liquid can be pumped through the reservoir 5 to a vent passage 17 and the reservoir 5 are thereby filled.
- valve 15 is closed and the valves 14 and 16 are opened and the Aktu réelleshuntn 6a, 6b and 6c not actuated.
- the valves 14 and 16 can then be closed and the liquid in the reservoir is now "on chip.”
- the valve 15 can be opened while the valves 14 and 16 remain closed
- the actuation chamber 6b, and finally the actuation chamber 6c can be evacuated and the reservoir 5 can be basically and not only limited to this embodiment also two or more than three actuation chambers 6 in FIG microfluidic component 1.
- an amount of liquid precisely defined in the volume can be measured and stored in advance
- the quantity of liquid provided can then be controlled, if necessary also in several defined S are discharged from the reservoir 5, without an external pump is needed.
- FIG. 7 shows a schematic cross section along the line CC through FIG. 6 through the actuation chamber 6b and its supply channel 8b.
- the height of the Aktu istshuntn 6 T1 according to the invention in particular> 100 ⁇ to ⁇ 1000 ⁇ , for example, 250 ⁇ or 500 ⁇ , amount.
- the fluid chamber 5 can also have a height T2 of> 100 ⁇ m to ⁇ 1000 ⁇ m, for example 250 ⁇ m or 500 ⁇ m.
- FIG. 8 shows a simplified schematic plan view of a microfluidic component 1, in particular a peristaltic pump, in which the
- Actuating chambers are replaced by alternative Aktu réelleskomponenten 18a, 18b, 18c.
- the actuation components 18a, 18b, 18c may, for example, comprise actuators which can act on the elastic membrane directly or via plungers.
- the actuators may be piezoelectric, electromagnetic, electrostatic or shape memory actuators in this embodiment, for example.
- the fluidic substrate 2, the elastic membrane 3 and the drive substrate 4 are not shown in this illustration for the sake of clarity. According to the invention, only one pumping chamber 5 is advantageously provided, which not only simplifies the production of the fluidic substrate, but also a minimal fluidic resistance and a pumping rate which is increased in relation to the size can be achieved.
- the pumping chamber 5 can through the channels 1 1 a and 1 1 b, or through the inlet 10 a, filled and emptied through the outlet 10b.
- a fluid can be fed, for example through the channel 1 1 a in the pumping chamber 5, flow through them and leave through the channel 1 1 b the pumping chamber 5 again. Directed fluid flow may be accomplished by the controlled sequential actuation of the actuation components 18a, 18b, 18c.
- Fig. 9 shows a schematic cross section along the line D-D 'through Fig. 8.
- the fluidic substrate 2 the elastic membrane 3 and the
- the Aktu istskomponenten are designed as a plunger, which in turn act directly on the elastic membrane 3.
- the external actuators can be, for example, piezoelectric, electromagnetic, electrostatic or shape memory actuators.
- Examples 1 to 6 give in tabular form particularly suitable Aktuleitersmuster a peristaltic pump according to the invention, as shown for example in Figure 1 again.
- the Aktutechniksmuster are independent of the type of Aktutechnik and can for example be applied to an embodiment of the invention, as shown in Figures 8 and 9.
- the invention provides a microfluidic component, in particular a peristaltic micropump, with an optimized design. Due to the design according to the invention, a minimum dead volume and, furthermore, a higher pumping rate relative to the size can be achieved. In particular, by virtue of the inventively minimized dead volume, by the provision of only one fluid chamber, in particular pumping chamber, the pumpability of gases can be ensured and the required amounts of sample material and reagents can be kept low.
Landscapes
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Reciprocating Pumps (AREA)
Abstract
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102010028524A DE102010028524A1 (de) | 2010-05-04 | 2010-05-04 | Mikrofluidisches Bauteil, insbesondere peristaltische Mikropumpe, und Verfahren zu dessen Herstellung |
PCT/EP2011/053535 WO2011138070A1 (fr) | 2010-05-04 | 2011-03-09 | Elément microfluidique, en particulier micropompe péristaltique, et son procédé de réalisation |
Publications (2)
Publication Number | Publication Date |
---|---|
EP2567092A1 true EP2567092A1 (fr) | 2013-03-13 |
EP2567092B1 EP2567092B1 (fr) | 2017-05-10 |
Family
ID=43920153
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP11709089.4A Active EP2567092B1 (fr) | 2010-05-04 | 2011-03-09 | Elément microfluidique, en particulier micropompe péristaltique, et son procédé de réalisation |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP2567092B1 (fr) |
DE (1) | DE102010028524A1 (fr) |
WO (1) | WO2011138070A1 (fr) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102012212650A1 (de) | 2012-07-19 | 2014-01-23 | Robert Bosch Gmbh | Mikrofluidische Lagerungsvorrichtung zum Vorlagern eines Fluids, Verfahren zu dessen Herstellung und eine Verwendung derselben |
DE102013220445B4 (de) * | 2013-10-10 | 2016-04-07 | Robert Bosch Gmbh | Auslaufschutzeinheit für eine mikrofluidische Vorrichtung, mikrofluidische Vorrichtung, Verfahren zum Betreiben einer solchen Auslaufschutzeinheit und Verfahren zum Herstellen einer solchen Auslaufschutzeinheit |
WO2023170153A1 (fr) * | 2022-03-09 | 2023-09-14 | Hnp Mikrosysteme Gmbh | Système fluidique et procédé de fonctionnement d'un système fluidique |
DE102022211171A1 (de) | 2022-10-21 | 2024-05-02 | Robert Bosch Gesellschaft mit beschränkter Haftung | Versiegelung für eine mikrofluidische Kammer |
DE102022211170A1 (de) | 2022-10-21 | 2024-05-02 | Robert Bosch Gesellschaft mit beschränkter Haftung | Kompartiment mit Versiegelung |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100421359B1 (ko) * | 2001-07-24 | 2004-03-06 | 엘지전자 주식회사 | 탄성 재질의 기판 내에서 유체를 이송하는 방법 및 이를위한 장치 |
DE10238600A1 (de) * | 2002-08-22 | 2004-03-04 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Peristaltische Mikropumpe |
WO2009065427A1 (fr) * | 2007-11-23 | 2009-05-28 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Ensemble pompe avec soupape de sécurité |
US8080220B2 (en) * | 2008-06-20 | 2011-12-20 | Silverbrook Research Pty Ltd | Thermal bend actuated microfluidic peristaltic pump |
-
2010
- 2010-05-04 DE DE102010028524A patent/DE102010028524A1/de not_active Withdrawn
-
2011
- 2011-03-09 EP EP11709089.4A patent/EP2567092B1/fr active Active
- 2011-03-09 WO PCT/EP2011/053535 patent/WO2011138070A1/fr active Application Filing
Non-Patent Citations (1)
Title |
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See references of WO2011138070A1 * |
Also Published As
Publication number | Publication date |
---|---|
EP2567092B1 (fr) | 2017-05-10 |
DE102010028524A1 (de) | 2011-11-10 |
WO2011138070A1 (fr) | 2011-11-10 |
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