EP2464975A1 - Procédé pour séparer des particules et/ou des cellules présentant au moins deux spécificités de surface - Google Patents

Procédé pour séparer des particules et/ou des cellules présentant au moins deux spécificités de surface

Info

Publication number
EP2464975A1
EP2464975A1 EP10754252A EP10754252A EP2464975A1 EP 2464975 A1 EP2464975 A1 EP 2464975A1 EP 10754252 A EP10754252 A EP 10754252A EP 10754252 A EP10754252 A EP 10754252A EP 2464975 A1 EP2464975 A1 EP 2464975A1
Authority
EP
European Patent Office
Prior art keywords
particles
separation
magnetizable
cells
scavenger
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP10754252A
Other languages
German (de)
English (en)
Inventor
Hans-Werner Heinrich
Jan-Michael Heinrich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pluriselect GmbH
Original Assignee
Pluriselect GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pluriselect GmbH filed Critical Pluriselect GmbH
Publication of EP2464975A1 publication Critical patent/EP2464975A1/fr
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/72—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Definitions

  • the invention relates to a method for the separation of particles and / or cells with 2 and more surface specificities. Areas of application of the invention are medicine and pharmacy
  • Process step for the research, diagnosis and treatment of diseases For the separation of particles of different specific gravity (e.g.
  • lymphocytes e.g. are accompanied by the expression of typical structures on the outer cell membrane (such as the so-called Cluster of Differentiation - CDs).
  • Antibodies can be generated against these structures, which are the crucial tool for the more specific
  • FACS Fluorescence Activated Cell Sorting
  • the invention solves this problem by a novel combination of magnetic and scavenger-based separation process. It is realized according to claims 1-9.
  • cells with the characteristics A 1 B and C are to be isolated from blood.
  • the antibodies to A become particles with a
  • the blood sample is now pre-incubated with the detection systems A and C for 10 minutes. Thereafter, detection system B is added and incubated for an additional 10 minutes. The sample is then passed through a sieve cascade with 2 sieves
  • the screen 40 ⁇ m holds back: A, AB, AC, ABC
  • the 40 ⁇ m fraction is divided into: A, AB and AC, ABC
  • the 20 ⁇ m fraction is separated into the homogeneous fractions B and BC
  • Process step 3 The fractions A, AB and AC, ABC are prepared by known methods from
  • the method can be performed with large numbers of cells, little effort in a short time and low-stress for the cells.
  • One skilled in the art can make wide combinations through the appropriate composition of magnetic and size-defined ones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Electrochemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

La présente invention concerne un procédé simple permettant une séparation soigneuse de cellules et/ou de particules contenues dans des liquides, de préférence le sang. À cet effet, des étapes connues de procédés de séparation magnétique sont combinées au procédé de séparation par filtrage de particules de piégeage.
EP10754252A 2009-08-14 2010-07-16 Procédé pour séparer des particules et/ou des cellules présentant au moins deux spécificités de surface Pending EP2464975A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102009037331A DE102009037331A1 (de) 2009-08-14 2009-08-14 Verfahren zur Separierung von Partikeln und/oder Zellen mit 2 und mehr Oberflächenspezifitäten
PCT/DE2010/000830 WO2011018067A1 (fr) 2009-08-14 2010-07-16 Procédé pour séparer des particules et/ou des cellules présentant au moins deux spécificités de surface

Publications (1)

Publication Number Publication Date
EP2464975A1 true EP2464975A1 (fr) 2012-06-20

Family

ID=42985357

Family Applications (1)

Application Number Title Priority Date Filing Date
EP10754252A Pending EP2464975A1 (fr) 2009-08-14 2010-07-16 Procédé pour séparer des particules et/ou des cellules présentant au moins deux spécificités de surface

Country Status (10)

Country Link
US (1) US20120164634A1 (fr)
EP (1) EP2464975A1 (fr)
JP (1) JP2013501924A (fr)
KR (1) KR20120051738A (fr)
CN (1) CN102549431A (fr)
AU (1) AU2010281997A1 (fr)
CA (1) CA2771116A1 (fr)
DE (1) DE102009037331A1 (fr)
RU (1) RU2012109539A (fr)
WO (1) WO2011018067A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011118386A1 (de) 2011-11-14 2013-05-16 Pluriselect Gmbh Verfahren zur Isolierung von Zellen und Biopartikeln
JP2015533219A (ja) * 2012-10-11 2015-11-19 オルゲンテック ディアグノスティカ ゲゼルシャフト ミット ベシュレンクテル ハフツング 磁化可能なビーズを用いた検体検出及び検体濃度測定
WO2015176018A1 (fr) * 2014-05-15 2015-11-19 Cristian Ionescu-Zanetti Procédés et systèmes pour la séparation de cellules à l'aide d'une séparation magnétique et sur base de la taille
EP3263693A4 (fr) 2015-02-27 2018-02-21 Toppan Printing Co., Ltd. Procédé de séparation de cellules et dispositif associé
WO2023204043A1 (fr) * 2022-04-20 2023-10-26 ソニーグループ株式会社 Procédé et support composite pour isoler des particules biologiques

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5409813A (en) * 1993-09-30 1995-04-25 Systemix, Inc. Method for mammalian cell separation from a mixture of cell populations
US6994971B1 (en) * 1999-10-08 2006-02-07 University Of Utah Research Foundation Particle analysis assay for biomolecular quantification
GB0013658D0 (en) * 2000-06-05 2000-07-26 Dynal Asa Nucleic acid isolation
JP4407068B2 (ja) * 2001-03-26 2010-02-03 横河電機株式会社 磁気粒子泳動方法および装置
GB0110172D0 (en) * 2001-04-25 2001-06-20 Pa Consulting Services Improved analytical test approach for blood
US7166443B2 (en) * 2001-10-11 2007-01-23 Aviva Biosciences Corporation Methods, compositions, and automated systems for separating rare cells from fluid samples
WO2006012886A1 (fr) * 2004-07-30 2006-02-09 Hans-Werner Heinrich Procede et dispositif pour isoler des cellules, des bioparticules et/ou des molecules de liquides pour une utilisation pour les animaux, en biotechnologie (y compris la recherche biologique) et pour le diagnostic medical
US20070059680A1 (en) * 2005-09-15 2007-03-15 Ravi Kapur System for cell enrichment
JP2009511001A (ja) * 2005-09-15 2009-03-19 アルテミス ヘルス，インク． 細胞及びその他の粒子を磁気濃縮するためのデバイス並びに方法
FR2917174B1 (fr) * 2007-06-08 2021-02-12 Bio Rad Pasteur Analyse multiple d'echantillons sanguins

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2011018067A1 *

Also Published As

Publication number Publication date
JP2013501924A (ja) 2013-01-17
AU2010281997A1 (en) 2012-04-05
DE102009037331A1 (de) 2011-03-03
WO2011018067A1 (fr) 2011-02-17
KR20120051738A (ko) 2012-05-22
US20120164634A1 (en) 2012-06-28
CA2771116A1 (fr) 2011-02-17
CN102549431A (zh) 2012-07-04
RU2012109539A (ru) 2013-09-20

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