EP2464975A1 - Verfahren zur separierung von partikeln und/oder zellen mit 2 und mehr oberflächenspezifitäten - Google Patents
Verfahren zur separierung von partikeln und/oder zellen mit 2 und mehr oberflächenspezifitätenInfo
- Publication number
- EP2464975A1 EP2464975A1 EP10754252A EP10754252A EP2464975A1 EP 2464975 A1 EP2464975 A1 EP 2464975A1 EP 10754252 A EP10754252 A EP 10754252A EP 10754252 A EP10754252 A EP 10754252A EP 2464975 A1 EP2464975 A1 EP 2464975A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- particles
- separation
- magnetizable
- cells
- scavenger
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
- G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/72—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
Definitions
- the invention relates to a method for the separation of particles and / or cells with 2 and more surface specificities. Areas of application of the invention are medicine and pharmacy
- Process step for the research, diagnosis and treatment of diseases For the separation of particles of different specific gravity (e.g.
- lymphocytes e.g. are accompanied by the expression of typical structures on the outer cell membrane (such as the so-called Cluster of Differentiation - CDs).
- Antibodies can be generated against these structures, which are the crucial tool for the more specific
- FACS Fluorescence Activated Cell Sorting
- the invention solves this problem by a novel combination of magnetic and scavenger-based separation process. It is realized according to claims 1-9.
- cells with the characteristics A 1 B and C are to be isolated from blood.
- the antibodies to A become particles with a
- the blood sample is now pre-incubated with the detection systems A and C for 10 minutes. Thereafter, detection system B is added and incubated for an additional 10 minutes. The sample is then passed through a sieve cascade with 2 sieves
- the screen 40 ⁇ m holds back: A, AB, AC, ABC
- the 40 ⁇ m fraction is divided into: A, AB and AC, ABC
- the 20 ⁇ m fraction is separated into the homogeneous fractions B and BC
- Process step 3 The fractions A, AB and AC, ABC are prepared by known methods from
- the method can be performed with large numbers of cells, little effort in a short time and low-stress for the cells.
- One skilled in the art can make wide combinations through the appropriate composition of magnetic and size-defined ones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102009037331A DE102009037331A1 (de) | 2009-08-14 | 2009-08-14 | Verfahren zur Separierung von Partikeln und/oder Zellen mit 2 und mehr Oberflächenspezifitäten |
PCT/DE2010/000830 WO2011018067A1 (de) | 2009-08-14 | 2010-07-16 | Verfahren zur separierung von partikeln und/oder zellen mit 2 und mehr oberflächenspezifitäten |
Publications (1)
Publication Number | Publication Date |
---|---|
EP2464975A1 true EP2464975A1 (de) | 2012-06-20 |
Family
ID=42985357
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP10754252A Pending EP2464975A1 (de) | 2009-08-14 | 2010-07-16 | Verfahren zur separierung von partikeln und/oder zellen mit 2 und mehr oberflächenspezifitäten |
Country Status (10)
Country | Link |
---|---|
US (1) | US20120164634A1 (de) |
EP (1) | EP2464975A1 (de) |
JP (1) | JP2013501924A (de) |
KR (1) | KR20120051738A (de) |
CN (1) | CN102549431A (de) |
AU (1) | AU2010281997A1 (de) |
CA (1) | CA2771116A1 (de) |
DE (1) | DE102009037331A1 (de) |
RU (1) | RU2012109539A (de) |
WO (1) | WO2011018067A1 (de) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102011118386A1 (de) | 2011-11-14 | 2013-05-16 | Pluriselect Gmbh | Verfahren zur Isolierung von Zellen und Biopartikeln |
EA201500377A1 (ru) * | 2012-10-11 | 2015-10-30 | Оргентек Диагностика Гмбх | Способ обнаружения анализируемого вещества и определение его концентрации с использованием намагничиваемых гранул |
CN107075556B (zh) * | 2014-05-15 | 2022-09-20 | 流动生物科学公司 | 使用基于磁性和尺寸的分离进行细胞分离的方法和系统 |
WO2016136273A1 (ja) | 2015-02-27 | 2016-09-01 | 凸版印刷株式会社 | 細胞を分離する方法、およびそのためのデバイス |
WO2023204043A1 (ja) * | 2022-04-20 | 2023-10-26 | ソニーグループ株式会社 | 生体粒子分離方法、及び生体粒子分離用複合担体 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5409813A (en) * | 1993-09-30 | 1995-04-25 | Systemix, Inc. | Method for mammalian cell separation from a mixture of cell populations |
US6994971B1 (en) * | 1999-10-08 | 2006-02-07 | University Of Utah Research Foundation | Particle analysis assay for biomolecular quantification |
GB0013658D0 (en) * | 2000-06-05 | 2000-07-26 | Dynal Asa | Nucleic acid isolation |
JP4407068B2 (ja) * | 2001-03-26 | 2010-02-03 | 横河電機株式会社 | 磁気粒子泳動方法および装置 |
GB0110172D0 (en) * | 2001-04-25 | 2001-06-20 | Pa Consulting Services | Improved analytical test approach for blood |
US7166443B2 (en) * | 2001-10-11 | 2007-01-23 | Aviva Biosciences Corporation | Methods, compositions, and automated systems for separating rare cells from fluid samples |
KR101290558B1 (ko) * | 2004-07-30 | 2013-07-31 | 퓨리셀렉트 게엠베하 | 생물학적 연구를 포함하는 생명공학 및 의약적 진단학에서,동물에의 적용을 목적으로, 체액으로부터 세포, 생체 입자및/또는 분자를 분리하는 장치 및 방법 |
US20070059680A1 (en) * | 2005-09-15 | 2007-03-15 | Ravi Kapur | System for cell enrichment |
JP2009511001A (ja) * | 2005-09-15 | 2009-03-19 | アルテミス ヘルス,インク. | 細胞及びその他の粒子を磁気濃縮するためのデバイス並びに方法 |
FR2917174B1 (fr) * | 2007-06-08 | 2021-02-12 | Bio Rad Pasteur | Analyse multiple d'echantillons sanguins |
-
2009
- 2009-08-14 DE DE102009037331A patent/DE102009037331A1/de not_active Ceased
-
2010
- 2010-07-16 EP EP10754252A patent/EP2464975A1/de active Pending
- 2010-07-16 AU AU2010281997A patent/AU2010281997A1/en not_active Abandoned
- 2010-07-16 US US13/390,408 patent/US20120164634A1/en not_active Abandoned
- 2010-07-16 WO PCT/DE2010/000830 patent/WO2011018067A1/de active Application Filing
- 2010-07-16 KR KR1020127006546A patent/KR20120051738A/ko not_active Application Discontinuation
- 2010-07-16 JP JP2012524109A patent/JP2013501924A/ja active Pending
- 2010-07-16 CN CN201080044036XA patent/CN102549431A/zh active Pending
- 2010-07-16 RU RU2012109539/15A patent/RU2012109539A/ru not_active Application Discontinuation
- 2010-07-16 CA CA2771116A patent/CA2771116A1/en not_active Abandoned
Non-Patent Citations (1)
Title |
---|
See references of WO2011018067A1 * |
Also Published As
Publication number | Publication date |
---|---|
US20120164634A1 (en) | 2012-06-28 |
AU2010281997A1 (en) | 2012-04-05 |
RU2012109539A (ru) | 2013-09-20 |
JP2013501924A (ja) | 2013-01-17 |
WO2011018067A1 (de) | 2011-02-17 |
DE102009037331A1 (de) | 2011-03-03 |
KR20120051738A (ko) | 2012-05-22 |
CN102549431A (zh) | 2012-07-04 |
CA2771116A1 (en) | 2011-02-17 |
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